LOCUS       NZ_CP067254             2101 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain ESBL_DR28 plasmid pESBL_DR28d, complete
            sequence.
ACCESSION   NZ_CP067254
VERSION     NZ_CP067254.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214844
            Assembly: GCF_017639045.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 03:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,860
            CDSs (total)                      :: 4,741
            Genes (coding)                    :: 4,497
            CDSs (with protein)               :: 4,497
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 84 of 244
            Pseudo Genes (incomplete)         :: 158 of 244
            Pseudo Genes (internal stop)      :: 52 of 244
            Pseudo Genes (multiple problems)  :: 49 of 244
            Pseudo Genes (short protein)      :: 4 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ESBL_DR28"
                     /isolation_source="imported food: chilli pepper"
                     /db_xref="taxon:562"
                     /plasmid="pESBL_DR28d"
                     /geo_loc_name="Switzerland: Zurich"
                     /collection_date="2014"
                     /collected_by="ILS, University of Zurich"
     gene            399..1364
                     /locus_tag="JJB16_RS23945"
                     /old_locus_tag="JJB16_23940"
     CDS             399..1364
                     /locus_tag="JJB16_RS23945"
                     /old_locus_tag="JJB16_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1368..1634
                     /locus_tag="JJB16_RS23950"
                     /old_locus_tag="JJB16_23945"
     CDS             1368..1634
                     /locus_tag="JJB16_RS23950"
                     /old_locus_tag="JJB16_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          793..1024
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ggttttgttt ttattccctg ttttagtgat cgggtgtgtg gaaaaggttg gggtaagccg
       61 ttcgggggtg cttgttttgg ggggttaaaa ttgtggttat tttttgcgca attctcgcgc
      121 gtgatccttg tatttatact taagggataa atggcggata tgaaatagtg gtttagccca
      181 gtaatgacga ggctttgagt gggttttgac aggtcaaaga aaatggagca gaattgaggc
      241 gtttttaatc ggcgttgggg agtgcgtcaa cactccccaa catttcgaat gtgtcacctc
      301 agcggcaaac tctggtgaca tgtactggct cgcaatgcac aggtacgtga tgaatatacc
      361 acatcaaatc acagcctgcc cagatcggag caggcttaat gtcagaagat aaattccttt
      421 cggactacag cccccgtgat gcagtttggg atacccagcg cacgcttacc gattctgtcg
      481 ggggtatcta ccagactgct gctgaattcg agcgctatgc actccgtatg gcctcctgta
      541 gcggtttgtt acgttttggt tggtctacca tcatggaaac cggagaaacg cgcctacggc
      601 ttcgtagtgc gcaattttgc cgtgtccgtc attgccctgt ctgccagtgg agaagaaccc
      661 tcatgtggca agcccgtttt tatcaggctc taccgaaaat cgttgtggat tacccgtctt
      721 cccgatggtt gtttctgacg ttaactgtca ggaactgcga gataggtgaa cttggaacag
      781 tccttacagc aatgaatgcg gcgtttaagc gaatggaaaa gcgaaaggag ctatcacctg
      841 ttcaggggtg gatcagggct acggaggtga cgcgaggtaa ggatggcagc gcacatccgc
      901 attttcactg tctgctgatg gtgcaacctt cttggtttaa agggaagaac tacgttaagc
      961 acgaacgttg ggtagaactc tggcgcgatt gcttgcgggt gaactatgag ccgaatatcg
     1021 atattcgggc agtaaaaact aagacaggtg aggttgtggc caacgttgcc gagcaactgc
     1081 aaagcgcggt tgctgaaacg ctgaaatact ccgttaaacc ggaagatatg gcaaacgatc
     1141 ctgagtggtt tcttgagctg acgcggcagc ttcacaagcg ccgttttatc tcgaccggtg
     1201 gggcgctaaa aaacgtcctc cagttggatc gagaaaccaa tgaggatctt gtcattgccg
     1261 acgatgtagg ggatggcact gatgacggga agcggacggc gtttgtctgg gattcaggta
     1321 aacggcgtta caaacgcgcc cctgagaagg ataaatcgga ttaacgtatg aatattaata
     1381 ttgaatacct gaatggaaat aagactattg gtttattttt tttaagaagt gaagcggtga
     1441 ttcctgacag gtttaaaaac cttattttgc ttattgatgg attaagtttt ggcacatttg
     1501 gttttcatcc gcacgaaggt tttgaggatg aattaatttt atatattcag aaaacaaacg
     1561 agagggtaaa aactcttttt gtgaaaatag atttaaacgg tatcaagttt ggatttttaa
     1621 gaacgcattc ttagttctgg aaaagagcca gcggcaggct gaggtgatag gtacgagatt
     1681 gcatgcaatc tctagtgctc tgtctatcct gcattatcct cagcattatc ctcagcatta
     1741 tcctcagcct tgccaactcg acaccaatgc aggatagaca atccgatgtc aaatgttaac
     1801 actctgcgag tggtacattt tccccggatt atcgtcctga gcctgccgct ggctctcttt
     1861 ctaccgcctc gctttgctcg ttgctcaacg cctcacagac acggattaaa atccgcatcc
     1921 gttcaccgtt ttttaaagtc cgttaaaagc atgatgccat ctccgagagt taatctcgtc
     1981 aaatgctaaa tcgtgggggt cccctttggg gttccgattt agtgattgac gacaccaccg
     2041 attaaaaaac ttatgcgggg tggatggttt cacgaagtga ggccatccac ctgtaagaca
     2101 g
//
