LOCUS       NZ_CP067243             3902 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain P19_598 plasmid pP19_598b, complete
            sequence.
ACCESSION   NZ_CP067243
VERSION     NZ_CP067243.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214846
            Assembly: GCF_017639005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 03:32:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,016
            CDSs (total)                      :: 4,905
            Genes (coding)                    :: 4,753
            CDSs (with protein)               :: 4,753
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 60 of 152
            Pseudo Genes (incomplete)         :: 101 of 152
            Pseudo Genes (internal stop)      :: 29 of 152
            Pseudo Genes (multiple problems)  :: 33 of 152
            Pseudo Genes (short protein)      :: 4 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067243.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P19_598"
                     /isolation_source="surface water"
                     /db_xref="taxon:562"
                     /plasmid="pP19_598b"
                     /geo_loc_name="Switzerland: St. Gallen"
                     /collection_date="2019"
                     /collected_by="ILS, University of Zurich"
     gene            join(3592..3902,1..13)
                     /gene="mobA"
                     /locus_tag="JJB18_RS24770"
                     /old_locus_tag="JJB18_24765"
     CDS             join(3592..3902,1..13)
                     /gene="mobA"
                     /locus_tag="JJB18_RS24770"
                     /old_locus_tag="JJB18_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            361..1716
                     /locus_tag="JJB18_RS24775"
                     /old_locus_tag="JJB18_24770"
     CDS             361..1716
                     /locus_tag="JJB18_RS24775"
                     /old_locus_tag="JJB18_24770"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_000498067.1"
                     /translation="MFVRLTIENFRSVKENFTLDLSASGSNSHLVNHIYKNAEMSVGTL
                     MSAGIYGANASGKSNVLMAFEAIKYIATDSGDLKEGTRIPCYEPYALSLATNESPVRFV
                     ADFYSVEGNRFNYEVKYIKDRIIFESLDYYPSRVKANLFTRDEGDTWETIKFGGHYRGG
                     VKKIPFFPNNSYLAKAGNNAASPDIIKEAYDFFRKGIRHIGLNEKIRISSFGKREEVVS
                     ETAEVLCMVDTGVAGISVREVESDLPIRMDDDIPPEIKEMIEEDYKYRYLFSHATEEGG
                     SVSFPMSRESEGTQKLFEIIPLIRSAFNNSMVVIIDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLIFSTHNMQLMTPEKMRRDQIWFCEKNKGASALYSLDDFDKKKVKTTTPYASW
                     YDEGRFGGVPDINYLKVAKFLSGNAMTAIHDIDVDALAGDFFEEFNEDLSND"
                     /name="ATP-binding protein"
     gene            1709..2326
                     /locus_tag="JJB18_RS24780"
                     /old_locus_tag="JJB18_24775"
     CDS             1709..2326
                     /locus_tag="JJB18_RS24780"
                     /old_locus_tag="JJB18_24775"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_000166785.1"
                     /translation="MTKRKGGRNVPRVMYIYCEGEKTEPLYLESYISENSRRTLSVFKI
                     PKTRKNTPEQLVDEAIKKKNSSSTADGDEFWVVYDQEHLTTQSVLCHQRAWNKANRHGI
                     NIAISCVCFELWLLLHFGYTTRSFSSYENLMSDSPFKGLLPNYNKGSSSTYDVLKNRIG
                     KARKNAAQLEIFAKSDNENIHEPYKFPCYTSFHKLLDAIDNF"
                     /name="RloB family protein"
     gene            complement(3126..3320)
                     /locus_tag="JJB18_RS24785"
                     /old_locus_tag="JJB18_24780"
     CDS             complement(3126..3320)
                     /locus_tag="JJB18_RS24785"
                     /old_locus_tag="JJB18_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041643.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3358..3583
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="99.0"
                     /evalue="4.9e-75"
     Typing          complement(2568..3097)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.413"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          3593..3900
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.029"
                     /coverage="96.0"
                     /evalue="2.84e-61"
ORIGIN
        1 tatggcagat tagcttcccg gagagaaaac tgtcgaaaac agacggtatg aacaccgtaa
       61 gctcccaaag tgatcaccat tcgctttcat gcatagctat gcagcgagct gaaaacgatc
      121 ctgacgcatc cttcctgttt tcccggggta aaacatctct ttttgcggtg tctgcgtcag
      181 aatcgcgttc agcgcgtttc agtggtgcgt acaatttata ggttatgtta aatgttgtgt
      241 ggtgaatggt cgtacaagat tctttatgat gatgttgttt caaatttaaa gcatgtgctg
      301 tatcttgttt attgcttttt tttcgctttt ttggcgaaaa aactcattta ggagttgcct
      361 atgtttgttc gtttgactat tgaaaatttt agatccgtaa aggaaaactt tacgcttgat
      421 ttaagtgcgt ctggctcaaa tagccattta gttaatcata tttataaaaa cgccgagatg
      481 tctgttggaa cacttatgag tgctgggatt tatggggcga atgcgtcagg taagtccaac
      541 gttctcatgg cttttgaggc tattaaatat attgctacag atagcgggga tctgaaggaa
      601 ggaactcgaa ttccatgcta cgaaccgtat gccctctcgt tggctacgaa tgagtcacca
      661 gttcggtttg tggctgattt ttactctgtt gaggggaatc gttttaatta tgaggttaaa
      721 tatattaaag atcgaattat attcgagtca ttggattact atccgtctag ggtaaaggct
      781 aatttgttta ctagggatga aggcgacaca tgggaaacaa ttaagtttgg tggtcattat
      841 agaggtgggg tgaaaaaaat accattcttc cctaataact catatttggc caaggccggt
      901 aataatgcgg catctccaga tataattaaa gaagcttatg acttttttag gaaagggata
      961 aggcatatag gtcttaatga aaaaataaga atctcatctt ttggtaaaag agaggaagta
     1021 gtaagtgaaa ccgctgaggt tctgtgcatg gttgatacag gtgttgctgg aatatccgtg
     1081 cgagaagtag aatctgattt accaataaga atggatgatg atattcctcc agaaataaaa
     1141 gaaatgatag aggaggatta taagtatcga tatttgtttt ctcatgcaac tgaggagggc
     1201 gggagtgttt cttttcctat gtccagagag tctgaaggca ctcagaagtt atttgaaata
     1261 atacctttaa ttagaagtgc atttaataac agcatggttg tgattatcga tgaactagat
     1321 aatagtttgc atccacatat tgcagatcta attgttaagc tatttaatga tcctgatgtt
     1381 aacaaaaagg gatctcagct aatattctct acgcataata tgcagttgat gactcctgaa
     1441 aaaatgagaa gggatcagat atggttttgc gaaaaaaaca aaggtgcttc ggcattatac
     1501 agtttagatg attttgataa gaaaaaagta aaaacaacaa caccttatgc tagttggtat
     1561 gatgagggcc ggtttggcgg cgttcctgat attaattatt tgaaagtggc taaattccta
     1621 tcaggaaatg ctatgactgc tattcatgat attgatgttg atgctcttgc tggtgatttt
     1681 ttcgaagagt ttaacgagga tctttctaat gactaaaagg aagggaggga gaaatgttcc
     1741 tcgagtgatg tatatatatt gtgaagggga gaagacggag ccgttgtatc ttgaatctta
     1801 tatatctgaa aattcaagaa gaacgttgag tgtgtttaaa atacctaaaa caaggaaaaa
     1861 tacaccagag cagctagtag atgaggcgat aaagaaaaag aattcatcat ccactgcgga
     1921 tggtgatgag ttttgggtcg tttatgatca agagcatttg actacgcagt ctgttttgtg
     1981 ccatcaaagg gcgtggaaca aagctaatag acacggtata aatatagcta tatcttgtgt
     2041 ttgttttgaa ctgtggttgt tgttgcactt tggttataca acaagatctt tttcatctta
     2101 tgaaaattta atgtctgact caccgtttaa gggtctgttg ccaaattata ataaaggtag
     2161 tagttcaaca tatgatgttt tgaagaatag gattgggaag gcgagaaaaa atgcggcgca
     2221 actagagatt ttcgctaaat cagataacga aaatatacat gagccatata aatttccatg
     2281 ttatacatca tttcataagt tacttgatgc gattgataac ttttgaatta atccattgac
     2341 catggcttat tgctggttgt aatctataga aaataaaaac caccgctacc aacggtggtt
     2401 tttagaaggt acatgagcta ccaacttatg taccgggtaa ctggcttaga ggagcgcagt
     2461 caccaaaaat tgtcctttta gtttagcctt aactggcgca tgacttcaag actaactcct
     2521 ctaaatcggt taccagtggc tgctgccagt ggcgttaagt cgtgtcttac cgggttggac
     2581 tcaagacgat agttaccgga taagacgcag cggtcgggct gaacgggggg ttcgtgcaca
     2641 cagcccagct tggagcgaac gacctacacc gggccgagat acctacagcg tgagctatga
     2701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2761 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2821 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2881 agcctatgga aaaacgcctg cggtgctggc ttcttccggt gctttgcttt ttgctcacat
     2941 gttctttccg gctttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3001 tgacaccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     3061 agagcgcctt atgtgacatt ttctccttac gctctgttgt gccgttcggc atcctgccct
     3121 gagcgttata tctctgtgct attttctact tcaaagcgtg tctgtatgct gttctggagt
     3181 tcttccgcga gttcgtgcag cttctcacac atggcggcct gttcgtcggc atcgagtgcg
     3241 tccagctttt cgagcagtgt taggctctgg ctttttatga atcccgccat gttgagtaca
     3301 gtttgctgct gcttgttcat ctttctgttt tctccgttct gtctgtcatc tgcgtcgtgt
     3361 gattatatcg cgcaccactt ttcgaccgtc ttaccgccgg tattctgccg acggatattt
     3421 cagtcagaca acactgtcac tgccaaaaaa cagcagtgct ttgttggtaa ttcgaacttg
     3481 cagacaggac aggatgtgca attgttatac cgcgcataca tgcacgctat tacaattacc
     3541 ctggtcaggg cttcgccccg acaccctatg tcagatacgg agccatgttt tatgacaaaa
     3601 cgaagtggaa gtaatacgcg caggcgggct atcagtcgcc ctgttcgtct gacggcagaa
     3661 gaagaccagg aaatcagaaa aagggctgct gaatgcggca agaccgtttc tggtttttta
     3721 cgggcggcag ctctcggtaa gaaagttaat tcactgactg atgaccgggt gctgaaagaa
     3781 gtcatgcgac tgggggcgtt gcagaaaaaa ctctttatcg acggcaagcg tgtcggggac
     3841 agggagtatg cggaggtgct gatcgctatt acggagtatc accgtgccct gttatccagg
     3901 ct
//
