LOCUS       NZ_CP067240             2295 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain SBF22 plasmid pSBF22e, complete sequence.
ACCESSION   NZ_CP067240
VERSION     NZ_CP067240.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214847
            Assembly: GCF_017638985.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 03:44:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,137
            CDSs (total)                      :: 5,018
            Genes (coding)                    :: 4,837
            CDSs (with protein)               :: 4,837
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 64 of 181
            Pseudo Genes (incomplete)         :: 128 of 181
            Pseudo Genes (internal stop)      :: 28 of 181
            Pseudo Genes (multiple problems)  :: 34 of 181
            Pseudo Genes (short protein)      :: 4 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067240.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SBF22"
                     /isolation_source="surface water"
                     /db_xref="taxon:562"
                     /plasmid="pSBF22e"
                     /geo_loc_name="Switzerland: Dietlikon"
                     /collection_date="2017"
                     /collected_by="ILS, University of Zurich"
     gene            complement(join(2245..2295,1..267))
                     /locus_tag="JJB19_RS25330"
                     /old_locus_tag="JJB19_25325"
     CDS             complement(join(2245..2295,1..267))
                     /locus_tag="JJB19_RS25330"
                     /old_locus_tag="JJB19_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023224510.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEATGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            1232..1423
                     /locus_tag="JJB19_RS25335"
                     /old_locus_tag="JJB19_25330"
     CDS             1232..1423
                     /locus_tag="JJB19_RS25335"
                     /old_locus_tag="JJB19_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(475..995)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.868"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gttagctgct tttttgcggg cttcgccttt tgccatgcca tttcctatcc caaaatcacc
       61 caggaaacct aatacggtgc gcccgtcaaa ttcgccggtt gcctcaattt cttgttgtac
      121 actgcgcgtt ttggcgattt ccagagctac cgctttgcaa tccattgcgg aagattctgg
      181 accggtgacg ttcgcggcct gtggatattg ctttgttgcg catcctgcca tgatcagcgc
      241 tgccgatagg atgaatattt ttttcaaaat tatccttatt tatttaggca tagttaagag
      301 atacccattt tatcatttag gcatagttaa gagataccca ttttatcggc aggaaatgag
      361 ggttttttag ttgaattttg ggaggggcaa ggttccatat tggtcgagga ttctggagct
      421 gaccggcaac ggccagcgcg gctatgggcc atgcggtggt tgagcataga aaggatcttc
      481 ttgagttcct ttttttctac gcgtaatctc ttgctctgaa aacgaaaaaa ccacctgggg
      541 aggtggtttt tcgaaggtta agtcagttgg ggaactgctt aacctggtaa ctggcttcag
      601 cagagcgcag ataccaaata ctgtccttcc agtgtagccg tagttaggcc accacttcaa
      661 gaactctata gcaccaccta aattaactcg ctctgctaat cctgttacca gtggctgctg
      721 ccagtggcgc tttgtcgtgt ctttccgggt tggactcaag acgatagtta ccggataagg
      781 cgcagcagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
      841 tcccggaact gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca
      901 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc
      961 tggtatcttt aagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
     1021 gcttgtcagg ggggcggagc ctatggaaaa acgccccgcc ggggcgtggc cttttcgccc
     1081 ttgtccttag ctttcgttat gcttaccgtt acgttatctg cttatcctcc ctccgctcgc
     1141 cgcagactca tgaccgaacg gagtgagcga atgagcgagg aagcggaaaa ccatccgaac
     1201 gtacagtaag cactttcgcg ccggggcttt catgaacaaa caacaaaaga ctgccgttaa
     1261 catggctaaa ttcatccagg atcagtccct gcttcttctg gacaggctta atgagctgga
     1321 tctggatcat gaggctgatt tctgcgagaa actccatgag gatgcggaac gcctttaccg
     1381 aagcctgtca gcgcgcctga atgagcagca ggacggtgcg taatggctcc gttctgggta
     1441 ttaccggagt aataccggca ttgccttaat tttcattgtg ttagcgctta acattattac
     1501 cccggtaata cccagaatcc ctgtattttt tcttttttat ttatttttca tgaagttgcg
     1561 atgaaaatcg cagctgcgtt aggtgtatga ccattttgag gttgaaaaat aatcaaccat
     1621 ggcgggtgat ttacgcaggt taaggggaga tcgtgacaaa aaaagcgttc ctcactgtat
     1681 cgttcatgcc tgcatgaacg ggttattctt cctgactaca gaattacatc gctccttcag
     1741 gccatacatc acttttacaa cacttttaca tcattccttc gctctgtaca tcacttttac
     1801 aacacttttg catcactttt acatcattcc ttcagctcat acgtcacttt tacgtcagtt
     1861 ccatcctcag tgccatttgt acatccgacc cccttcctga acgtgctgaa aacggttttt
     1921 ccggctgtgc cggcaacgcc tcacggccac gcaataaatt gcgtccccgt tcaccgtttt
     1981 cgggtaagaa gaacatcgga aacaggactt accccggctg aatggcgtga atttccgcgc
     2041 tatgcacttg cgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg
     2101 gttttcgggt ggtttgttgc ctgttttacc ggtttcccgt cagaaatgcc ctgagggcct
     2161 ctcagggggt gcgggcaaca gatgttatgg taaatccagc tgattggcag gtacaaaaaa
     2221 agcccggcag ggcttttttg taccttattg atctgcacac tttgcagcct tcagggcttc
     2281 cagctgattt agccg
//
