LOCUS       NZ_CP067239             3131 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain SBF22 plasmid pSBF22d, complete sequence.
ACCESSION   NZ_CP067239
VERSION     NZ_CP067239.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214847
            Assembly: GCF_017638985.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 03:44:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,137
            CDSs (total)                      :: 5,018
            Genes (coding)                    :: 4,837
            CDSs (with protein)               :: 4,837
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 64 of 181
            Pseudo Genes (incomplete)         :: 128 of 181
            Pseudo Genes (internal stop)      :: 28 of 181
            Pseudo Genes (multiple problems)  :: 34 of 181
            Pseudo Genes (short protein)      :: 4 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067239.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SBF22"
                     /isolation_source="surface water"
                     /db_xref="taxon:562"
                     /plasmid="pSBF22d"
                     /geo_loc_name="Switzerland: Dietlikon"
                     /collection_date="2017"
                     /collected_by="ILS, University of Zurich"
     gene            318..551
                     /locus_tag="JJB19_RS25305"
                     /old_locus_tag="JJB19_25300"
     CDS             318..551
                     /locus_tag="JJB19_RS25305"
                     /old_locus_tag="JJB19_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015675076.1"
                     /translation="MSVDFCDARQGGGAYGKTPAVLASSGALLFAHMFFPALSPDSVDN
                     RITAFECADTARRSRTTERSESVSEEAEERLM"
                     /name="hypothetical protein"
     gene            complement(601..795)
                     /locus_tag="JJB19_RS25310"
                     /old_locus_tag="JJB19_25305"
     CDS             complement(601..795)
                     /locus_tag="JJB19_RS25310"
                     /old_locus_tag="JJB19_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041644.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1068..1391
                     /gene="mobA"
                     /locus_tag="JJB19_RS25315"
                     /old_locus_tag="JJB19_25310"
     CDS             1068..1391
                     /gene="mobA"
                     /locus_tag="JJB19_RS25315"
                     /old_locus_tag="JJB19_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_208776487.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDLEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSKL
                     MAD"
                     /name="mobA"
     gene            1850..2602
                     /locus_tag="JJB19_RS25320"
                     /old_locus_tag="JJB19_25315"
     CDS             1850..2602
                     /locus_tag="JJB19_RS25320"
                     /old_locus_tag="JJB19_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_208776486.1"
                     /translation="MDTTSLISSISVPRLSFYETFLGCATDQEKVGAYVAYQDLSGDFF
                     CLVQMIEVALRNAINNTIKTNHGPAWYDSIPQTKKSQDLVLNAKQKALDECGVRYTDDD
                     VICRLTFGFWVYMLDAPYRDTQRPCYIWTPENKAKTFPHAKNPLGGGDMKVKALFDEFH
                     KVLLFRNRLFHHEPIWKKHHCKSHSQAINNMLKEFNFLMNALSIVSNEKKELIEFIGHD
                     RRFYERCTIEYVMGIIGRIKCRELKVAG"
                     /name="hypothetical protein"
     gene            complement(2627..2770)
                     /locus_tag="JJB19_RS25325"
                     /old_locus_tag="JJB19_25320"
     CDS             complement(2627..2770)
                     /locus_tag="JJB19_RS25325"
                     /old_locus_tag="JJB19_25320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152954547.1"
                     /translation="MNTKVLFVKKALKSITCATLSLLPALSRAVTELRRLSAARLKAGK
                     SF"
                     /name="hypothetical protein"
     Typing          833..1059
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.382"
                     /coverage="99.0"
                     /evalue="2.9500000000000003e-72"
     Typing          complement(43..572)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.041"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1069..1388
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="3.24e-61"
ORIGIN
        1 tcagttacca gtggctgctg ccaatggcga taagtcgtgc ctttccgggt tggactcaag
       61 acgatagtta ccggataagg cgcagcagtc gggctgaacg gggggttcgt gcacacagcc
      121 cagcttggag cgaacgacct acaccgggcc gagataccta cagcgtgagc tatgagaaag
      181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaaacggca gggtcggaac
      241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      301 ggttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      361 atggaaaaac gcctgcggtg ctggcttctt ccggtgcttt gctttttgct cacatgttct
      421 ttccggcttt atcccctgat tctgtggata accgtattac cgcctttgag tgcgctgaca
      481 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc
      541 gccttatgtg acattttctc cttacgctct gttgtgccgt tcggcatcct gccctgagcg
      601 ttatatctct gtgctatttt ctacttcaaa gcgtgtctgt atgctgttct ggagttcttc
      661 tgcgagttcg tgcagcttct cacacatggt ggcctgctcg tcagcatcga gtgcgtccag
      721 tttttcgagc agcgtcaggc tctggctttt tatgaatccc gccatgttga gtacagtttg
      781 ctgctgcttg ttcatctttc tgttttctcc gttctgtctg tcatctgcgt cgtgtgatta
      841 tatcgcgcac cacttttgga ccgtcttacc gccggtattc tgccgacgga catttcagtc
      901 agacaacact gtcactgcca aaaaaacagc agtgctttgt tggtaattcg aacttgcaga
      961 caggacagga tgtgcaattg ttataccgcg catacatgca cgctattaca attaccctag
     1021 tcagggcttc gccccgacac cccatgtcag atacggagcc atgttttatg acaaaacgaa
     1081 gtggaagtaa tacgcgcagg cgggctatca gtcgccctgt tcgtctgacg gcagaagaag
     1141 acctggaaat cagaaaaagg gctgctgaat gcggcaagac cgtttctggt tttttacggg
     1201 cggcagctct cggtaagaaa gttaactcac tgactgatga tcgggtactg aaagaagtta
     1261 tgagactggg ggcgttacag aaaaaactct ttatcgacgg caagcgtgtc ggggacagag
     1321 agtatgcgga ggtgctgatc gctattacgg agtatcaccg ggccctgtta tccaagctta
     1381 tggcagatta gcttcccgga gagaaactgt cgaaaacaga cggtatgaac gccgtaagcc
     1441 cccaaaccga tcgccattca ctttcatgca tagctatgca gtgagctgaa aacgatcctg
     1501 acgcattttt ccagttttca ctgggtaaaa catctctttt tgcggtgttt gcggcagaat
     1561 cgcgttcagc gcgttttagc ggtgcgcgta aggggagtta tggtaaattg gcagcaaagt
     1621 attttctgat gttatggatt gtgaagagta ttcaatcctt attatgaaac aataactata
     1681 actggttgtt tcatatagat aaatataaca aaaaagccca ctctgtagtg ggcttttttg
     1741 ttatatttta cgctctagag gtacacagaa aaacggtagt tcgcctgctc ctcgaatgtt
     1801 gtaatggtag tctgaaaaat attcatagta aagagataag tgagttttca tggatacaac
     1861 aagtttaatt agctcaattt ctgtaccaag attatctttc tatgaaacat ttcttgggtg
     1921 cgctactgat caagaaaagg ttggggcata cgttgcctac caagatctgt ctggcgattt
     1981 tttttgtctt gtccagatga ttgaggttgc tctgcgtaat gcgataaaca atacaatcaa
     2041 aactaatcat gggcctgctt ggtacgactc tatacctcaa acaaagaaat ctcaggatct
     2101 tgttttaaat gcgaaacaaa aagcactaga tgagtgtggc gtaaggtata cggatgatga
     2161 tgttatttgc cgtctaacct ttggcttttg ggtttatatg ttagatgccc catataggga
     2221 tacgcaaagg ccgtgctaca tctggactcc agagaacaag gcaaaaacat ttcctcatgc
     2281 gaagaatcct ttgggtgggg gggatatgaa ggttaaagcg ctgtttgacg agtttcataa
     2341 agttcttttg tttaggaatc gactttttca ccatgagcct atttggaaaa aacaccactg
     2401 caagtcgcat agtcaagcca taaataatat gttgaaagag ttcaattttt taatgaatgc
     2461 cttgagtatc gtctctaacg agaaaaagga acttattgag tttattgggc acgataggcg
     2521 cttttatgaa cgctgcacga tagagtatgt tatggggatt atagggagga ttaagtgcag
     2581 ggagcttaag gtagccggtt gacttttaag gaaaaatgtg atacaatcaa aagctcttac
     2641 cggctttaag tcgcgcagcc gaaaggcgac gtaactctgt tactgcgcga cttaaagccg
     2701 gtaagagcga gagagttgcg caagtaattg atttcaaagc ttttttcaca aaaagaactt
     2761 tagtattcat tttcataatc ctctatttct tatgccgttc taatggcagc gttgactttt
     2821 tattccatct atttgaatgg ttaattatac catatctaac atcaaatctt aaggataaat
     2881 agctgattca catcagtttt tccattaatt cacgaagttt tataatttaa ttacggattt
     2941 ccttgagttc atatcgcgcc catcgtaatc ttaaaaacga aaaaaccacc cggcaaggtg
     3001 gtttttcgaa ggttcagcaa ccggcaagtt tctgaaccgc ggtaactggc ttaagaggag
     3061 cgcagtcacc aaaacttgtt attccagttt agccttaact ggcacgtaac ttcaagacta
     3121 actcctctaa a
//
