LOCUS       NZ_CP067051             3269 bp    DNA     linear   CON 30-MAY-2024
DEFINITION  Klebsiella pneumoniae strain B6753 plasmid p_unnamed_4.
ACCESSION   NZ_CP067051
VERSION     NZ_CP067051.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16393502
            Assembly: GCF_015159775.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Hypervirulent Multidrug resistant Klebsiella pneumoniae VB6753
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 3269)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 81.6x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015159775.2-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:08:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,670
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,387
            CDSs (with protein)               :: 5,387
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 74 of 163
            Pseudo Genes (incomplete)         :: 96 of 163
            Pseudo Genes (internal stop)      :: 28 of 163
            Pseudo Genes (multiple problems)  :: 32 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067051.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B6753"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p_unnamed_4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            639..965
                     /locus_tag="IL988_RS28240"
                     /old_locus_tag="IL988_028125"
     CDS             639..965
                     /locus_tag="IL988_RS28240"
                     /old_locus_tag="IL988_028125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_227654993.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLADLDYENYIQVAQIDIADAL
                     RMQKTNVSRAIKNLIEFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALSNGLSVIQ
                     GGRS"
                     /name="helix-turn-helix domain-containing protein"
     gene            981..1319
                     /locus_tag="IL988_RS28245"
                     /old_locus_tag="IL988_028130"
     CDS             981..1319
                     /locus_tag="IL988_RS28245"
                     /old_locus_tag="IL988_028130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_134901817.1"
                     /translation="MKYFLTATAIVLLSGCVNSSGVLPMGPDTYSISVHAAPVRGLAGA
                     RKAALTEANQYCQSQGKSILVKNMDNSTFSSLPGDDVSIIFQCLSAGDNGLQRPIYGRP
                     ADINVNVN"
                     /name="hypothetical protein"
     gene            complement(1459..1692)
                     /locus_tag="IL988_RS28250"
                     /old_locus_tag="IL988_028135"
     CDS             complement(1459..1692)
                     /locus_tag="IL988_RS28250"
                     /old_locus_tag="IL988_028135"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_049595772.1"
                     /translation="MTELETHLLNALKRMEQQFSEQLKASEKAQAALQSMFERTAEDNA
                     TLLKQVSFLDNRVTVLSEQLKQFGNLYRQNRR"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            complement(1696..2202)
                     /locus_tag="IL988_RS28255"
                     /old_locus_tag="IL988_028140"
     CDS             complement(1696..2202)
                     /locus_tag="IL988_RS28255"
                     /old_locus_tag="IL988_028140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_134901815.1"
                     /translation="MSVSLTLQERLKQQHLAESETIQSLTKQQLSSLRSELNGIFQQEL
                     NTMLEDMRRQLHDTTEDMRQSRQSMLLTVVKNRVIFPALSAVTILAVIFLSGWGLSTFQ
                     ESRIMANWQQINSQKATLATLESKTAGLNIVQDKNGTFVVLPKGAKVDDSGWTVGKQQA
                     LRIVR"
                     /name="mobilization protein"
     gene            2438..2848
                     /locus_tag="IL988_RS28260"
     CDS             2438..2848
                     /locus_tag="IL988_RS28260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_242462300.1"
                     /translation="MVRLSNLTCAPWIIRVSQAIPGIDFTAQVIERLFCSISGRANVQA
                     LPGAKLNAGRHKMQLVMPGMGMPDPENVVLIGFKPRERDLFKAIHELPLHFRRDLLLRG
                     ERQHAGSVLVPEIERVDEFAGSIRRSPQYLWR"
                     /name="hypothetical protein"
     gene            complement(2935..3234)
                     /locus_tag="IL988_RS28265"
                     /old_locus_tag="IL988_028150"
     CDS             complement(2935..3234)
                     /locus_tag="IL988_RS28265"
                     /old_locus_tag="IL988_028150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_219199838.1"
                     /translation="MEKRDNMLRVRFSDAEFEALKQLAEDAGCTMSELVRDHLGRVSVR
                     NKDVDRERIAMLNRINANLNMIARWVNTHKSAASSVEVVAHLMDIERHIRELSR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(1365..2938)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.81"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggctacccga tgttgctaca tttcgctacg ctcaatgctg cacaccgggc cttgcaatgg
       61 gcggcttcgc ctccccttga cccctccctt gcagcagtcg ctttgctcct tcgcccaggg
      121 caacggcgtc tgtcaggtgg atggcctcac tgcgtgacac cacccacccc tgacaagcgt
      181 gctttgtccg gagagtcgtc aagggtgaaa tacactcgct acgctcgccc ttgacgaaat
      241 caggcaatac cagagccttt tgaatgagcc agtaccgctc gccgcagttc cggctgaggc
      301 gtaccgcagc gcagcgagga acgaacagcc ggaacgagga agcggaatat catctgaacg
      361 ggcatagaaa tgcgttcgct cgggaggaca tacactgtaa tcagtgttga agaggtaaca
      421 agatacagaa ggggatgtct cgcaagccgc gccagcaggg gttttgtttt gacaatagtt
      481 atctctcaag ataacttagt tatctctcaa gataactatc aggaagaaag cagatgcgga
      541 gagtcacaca gatagatctc gaaacagggg aggatttagg gggatttgta gctgtagtcc
      601 ggcctaagca gaagtcatca tttcagaggc atttcactat gaatcaagcg gcactgatca
      661 ctatcgccaa tgaactaaac catgatcaaa tgcgtgtgtt aatggctctc ttagctgacc
      721 ttgattacga aaattatatt caggtggctc agatagatat tgctgacgct ttgcggatgc
      781 agaagaccaa tgtcagtcga gctatcaaga atctaattga gtttggaata atccttgaag
      841 ggcctaaaat cgggcgcagt aagacttacc gcctgaaccc tcaatttggc tggaaaggca
      901 ctgttagtaa ccataaaaaa gccctctcaa atgggctgag cgtgatccaa ggtggacgct
      961 cctaaaacat aggattgaga atgaagtatt ttttaactgc aacagcaatt gttctgctca
     1021 gtgggtgtgt aaattctagt ggtgttctcc ctatggggcc tgacacctat tcaatttcag
     1081 tacacgccgc cccggtgcga ggtttggctg gagccaggaa ggcagcattg accgaagcta
     1141 atcagtactg ccagtcacag gggaaaagta ttctggtgaa aaatatggat aactcgacgt
     1201 ttagttcact gcctggtgat gacgtcagca ttattttcca atgcctttcc gcaggtgata
     1261 atggcctgca acgcccaatc tatggccgtc ctgctgatat aaacgttaat gttaattagt
     1321 atttaaaaat caggtatata taaatttagt atatgcctaa ttacatgctt ggcccgtagt
     1381 acctgtctgg cttcggcgcc ggaggctcag gttcatgctg tttctgctgc aatgcttcct
     1441 gaaactccag ctgccttctc agcgcctgtt ctgcctgtag aggttcccga actgcttcag
     1501 ctgctccgac aatactgtca cccgattgtc taagaagctg acttgcttca acagcgtggc
     1561 gttgtcttcc gcagtacgct cgaacatgct ctgcaaggcg gcctgcgcct tctcggaagc
     1621 tttcagctgc tcgctgaatt gctgctccat acgcttcaag gcgttcaata agtgcgtttc
     1681 cagttccgtc atggtttacc tcacgatccg cagggcttgc tgcttcccga ctgtccagcc
     1741 gctgtcgtcc actttcgccc ctttgggcag cacgacgaac gtcccgttct tgtcctgaac
     1801 gatgttcagg cccgctgttt tgctctccag cgtcgccagc gtggctttct ggctgttgat
     1861 ctgctgccag ttggccatga tccggctttc ctgaaacgta ctcaggcccc agccgctcag
     1921 aaagataacg gccaagatcg taaccgcgct cagggccggg aatatcaccc ggtttttcac
     1981 gacggtcagc aacatgctct gccggctctg gcgcatatcc tctgtcgtgt cgtgcagctg
     2041 ccggcgcata tcttccagca tggtattcag ctcttgctga aatatgccgt tcaactcgct
     2101 tcgcaaactg ctcagctgct gcttcgtcag gctttgaata gtctcgctct ctgcgagatg
     2161 ctgctgcttc aatcgctcct gcagcgtcaa actcacgctc ataaagtccc cctttcatgc
     2221 gccaccgctg gccgctctgc ggatctgaca cggtgatata atttttacct tcgcgcgtta
     2281 cctgcattcc aaggtctgaa acctgctcga taacgtcgct acggctgcgt ataagcccct
     2341 gtgctgccct ctgagacaaa accgcgtcta tgagtagcct gacgtcttcc tgcggcttct
     2401 cgccccttct gagggcttct gcggcgattt tcagctcatg gtcaggctga gcaatctcac
     2461 gtgcgcgccg tggatcatcc gggtcagcca ggccatacct ggcattgatt tcactgcgca
     2521 ggtcatcgaa cgcctgtttt gcagcatctc cgggcgggcg aatgttcagg ctcttcccgg
     2581 tgccaagctc aacgcggggc gtcacaaaat gcagctcgtg atgcccggca tgggtatgcc
     2641 tgacccagag aatgttgtac tgatcggctt caagccccgc gaacgcgacc tgttcaaagc
     2701 gatccatgaa ctcccgctcc atttccggcg tgaccttctc ctccggggcg aaagacagca
     2761 cgccggaagt gtacttgtgc cggaaatcga gcgagtcgat gagtttgcgg gtagcatcag
     2821 gcgatcccct cagtacctct ggcggtgaat cttcccgacc gggccgcgtt ttatcgatga
     2881 catagccgac cgggccagcg ccgccgccag tgccgtgcct gctgaaacct acaatcatcg
     2941 cgacagctcc cgaatatggc gctctatatc catcaggtga gcgacgacct caaccgagga
     3001 agccgccgat ttatgcgtat taacccagcg ggcaatcatg ttcaggttgg cattgatgcg
     3061 gttaagcatg gcaatacgtt cccgatcgac gtctttgttg cgcacgctga ccctgccgag
     3121 atgatcgcga accagctcgg acatggtgca gcctgcatct tcggccagct gtttcagcgc
     3181 ctcaaattcc gcgtcagaaa acctgacccg cagcatgtta tcgcgctttt ccattccacc
     3241 tccgtcgtcg aatattgcac attaaacaa
//
