LOCUS       NZ_CP067048             7418 bp    DNA     linear   CON 30-MAY-2024
DEFINITION  Klebsiella pneumoniae strain B6753 plasmid p_unnamed_1.
ACCESSION   NZ_CP067048
VERSION     NZ_CP067048.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16393502
            Assembly: GCF_015159775.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Hypervirulent Multidrug resistant Klebsiella pneumoniae VB6753
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 7418)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 81.6x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015159775.2-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:08:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,670
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,387
            CDSs (with protein)               :: 5,387
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 74 of 163
            Pseudo Genes (incomplete)         :: 96 of 163
            Pseudo Genes (internal stop)      :: 28 of 163
            Pseudo Genes (multiple problems)  :: 32 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067048.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B6753"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p_unnamed_1"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            822..1364
                     /locus_tag="IL988_RS28130"
                     /old_locus_tag="IL988_028020"
     CDS             822..1364
                     /locus_tag="IL988_RS28130"
                     /old_locus_tag="IL988_028020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001696621.1"
                     /translation="MNIDEVAKWRPFTDQDGNIWDLSFLDAHEVSYTHCSPGKPDIVYK
                     FTVSYSFHCFCKDYPEQTEEEKLSLMYDAGKDSRPFCKVRYALARDKLRAIIEGLGNAK
                     VIHAGYGSYAFVEITVDEGESEFYFVPFKVYRERKKLRLHVTSAYPVKERPSGKPVSFF
                     KIAHNLLRGKPLPKPHM"
                     /name="hypothetical protein"
     gene            complement(1910..1993)
                     /locus_tag="IL988_RS28400"
                     /old_locus_tag="IL988_028025"
     CDS             complement(1910..1993)
                     /locus_tag="IL988_RS28400"
                     /old_locus_tag="IL988_028025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058652899.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPDRHD"
                     /name="hypothetical protein"
     gene            complement(2614..3474)
                     /locus_tag="IL988_RS28135"
                     /old_locus_tag="IL988_028030"
     CDS             complement(2614..3474)
                     /locus_tag="IL988_RS28135"
                     /old_locus_tag="IL988_028030"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(3657..4214)
                     /locus_tag="IL988_RS28140"
                     /old_locus_tag="IL988_028035"
     CDS             complement(3657..4214)
                     /locus_tag="IL988_RS28140"
                     /old_locus_tag="IL988_028035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            4378..>7351
                     /locus_tag="IL988_RS28145"
                     /old_locus_tag="IL988_028040"
                     /pseudo=""
     CDS             4378..>7351
                     /locus_tag="IL988_RS28145"
                     /old_locus_tag="IL988_028040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     ARG             complement(2618..3474)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1885..2372)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.073"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 attacaaagg ttaaagcagc ggacaggaaa tgttttgtgc ccacagctat gctggcttat
       61 tcacaaaaca gcgggacaag atgtgttttt ggagtaccgc cggcccgcta aagactcctg
      121 gttagctccc gcatgtttgc tatctttttg ctatcttttt gctaccattt tgctactttt
      181 ttgctatctc cagagtcttc tggtctttcg gttgctatcc ttttgctact tttttgctat
      241 caaaatgcta cctctccctt cttgcaataa atgaccaagg cacgttagcg atgtatacat
      301 cgccgcgccg caagggtgcc ccttccgaat cccgttcatc actgcgtgct gattcatcca
      361 cgttaaatcc gaccccctta gcaataaatc cagccatgcc tgacggcctg gccaacgcct
      421 cacggccgca cagtaaactg cgcacccgtg aggcgttatt cgtgaggttc agtggtgctt
      481 ttgggcgtcc agccggattt tagccgctaa aatcccggcc atgcatgtgt gtgcatagcc
      541 atgcagacgc gccagaatca tcaggcgcgt tttttcacga tataggcaca gggaagtgtc
      601 ggaagcaggc aggaagaccc agaaacgccc tgagagcctc tcaggcggtg cacgcaacgg
      661 atgttatggt aaataacact taatcattac caatgtatcc atgaaaggtt gtggcgaagt
      721 cttctgtaaa aggcctagct cttattttgc cacgctcctt ccgcttgttt cttctctggt
      781 agaatgtgcg gattctgcca tccggatatt gaggtttttt cgtgaacatt gatgaagttg
      841 ctaagtggcg tccttttacg gatcaggatg gcaatatatg ggatctgtca tttcttgatg
      901 cacatgaggt ttcttatact cattgttctc ctggtaaacc tgatattgtg tataagttca
      961 ctgtttctta ctcatttcat tgtttttgca aggattatcc agagcagact gaggaagaaa
     1021 aattatcgtt gatgtatgat gcaggcaaag actcgaggcc attttgtaag gtccgatatg
     1081 cccttgctcg agacaagctg agagccatca ttgaaggatt gggtaacgcg aaggttatcc
     1141 atgctggata tggtagttat gcttttgtag agattactgt agatgaaggt gaaagtgagt
     1201 tctactttgt gccttttaag gtatatcgtg agcgtaaaaa gctgcgcttg catgtaacaa
     1261 gtgcttatcc tgtcaaggag agaccgtcag gtaagccagt tagctttttc aagatagctc
     1321 acaatctcct gcgcgggaag ccattaccta aacctcatat gtaacaaagc ccgcttagtt
     1381 gcgggcttcg ccagaaattt cttctggtat tcagaacacc tttaccgggc ttggcctcga
     1441 ccaacattgt tattggtagt gggatgtctg gtgttaccag tctgctgcat ccttaaagca
     1501 attgtaggta gtgtgatgta gatttgcaac caacattttg gttgcaacct atatactaat
     1561 ttttctcgat aatgccggaa tccattgctg tgtctggctt tcttgaagct cctgactata
     1621 gataccctat atcctaacag ttattctgct gtggatatca gaaatgaaca cttgagatca
     1681 tcagtggctc gtcgtagtct cttgctcaga aacgaaaaaa ccaccctggc aggtggtttt
     1741 ttcgaaggtt agttaatcct ggcagattct ctaaccgtgg taacagtctt gtgcgagaca
     1801 tgtcaccaaa tactgtcctt tcagtgtagc ctcagttagg ccgccacttc aagaactctc
     1861 gttacatctc tcgcacatcc tgcttaccag tggccgttgc caatggcgat tagtcgtgtc
     1921 tatccgggtt ggactcaaga cgatagttac cggacaaggc gcagcggtcg gactgaacgg
     1981 ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc
     2041 gtggaatgag ataaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga
     2101 acaggagagc gcacgaggga gccaccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2161 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc
     2221 ctatggaaaa acggcagcgc cgcatccttt tcctgccctg cctgtcacgc cgtatgcctt
     2281 tgtccttatc cgtgtgcagg taacgctcgc cgcagccgaa cgaccgagcg gagcgagtca
     2341 gtgagcgagg aagcggaata tctgcgggct tctctttggc accgtacgcg ccatagctgc
     2401 attttttaat acgatgcagg atagggcgaa cgccgcaaag tgacgtcacc tgacgttcct
     2461 ggattagggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     2941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3061 tgtcagaagt aagttggcag cagtgttatc actcatggtt atggcagcac tgcataattc
     3121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     3241 taccgcacca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3421 gcaaaatgcc gcaaaaaagg gaataagggc gacacgaaaa tgttgaatac tcatactctt
     3481 cctttttcaa tattattgaa gcatttacca gggttattgt ctcatgagcg gatacatatt
     3541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3601 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3661 gaggcccttt cgtcttcaag aattttataa accgtggagc gggcaatact gagctgatga
     3721 gcaatttccg ttgcaccagt gcccttctga tgaagcgtca gcacgacgtt cctgtccacg
     3781 gtacgcctgc ggccaaattt gattcctttc agctttgctt cctgtcggcc ctcattcgtg
     3841 cgttctagga tcctccggcg ttcagcctgt gccacagccg acaggatggt gaccaccatt
     3901 tgccccatat caccgtcggt actgatcccg tcatcaatga accggactgc cacgccctga
     3961 gcgtcaaatt cctttatcag ttggatcata tcggcagtgt cgcggccaag acggtcgagc
     4021 ttcttaacca gaatgacatc accttcctcc accttcatcc tcagcaaatc cagcccttcc
     4081 cgatctgttg aactgccgga tgccttgtcg gtaaagatgc ggttagcttt tacccctgca
     4141 tctttgagcg ctctgatctg aatatcgagg gactgctggc tggttgagac ccgcgcataa
     4201 ccaaaaattc gcataaaaat gtaccttaaa tcgaatatca gacacgatgt gtctattatg
     4261 ccaaaatgac gatttaatgg acactcaaac gaagccgttt tactatgtct gataatttat
     4321 aacatttcgt acggttggaa aaatgttact aaatgcccgt caggcaggga ggccgatatg
     4381 cccgttgact ttctgaccac tgagcagact gaaagctatg gcagattcac cggtgaaccg
     4441 gatgagcttc agctggcacg atattttcac cttgatgaag cagacaagga atttatcgga
     4501 aaaagcagag gtgatcacaa ccgtctgggc attgccctgc aaattggatg tgtccgtttt
     4561 ctgggcacct tcctcaccga tatgaatcat attccttccg gcgtccggca ttttaccgcc
     4621 agacagctcg ggattcgtga tatcaccgtt cttgcagaat acggtcagag ggaaaatacc
     4681 cgccgtgagc atgcagcgct gatacgtcag cactatcagt atcgtgaatt tgcctggccc
     4741 tggacatttc gccttacccg tcttttatat acccggagct ggataagcaa cgaacgtcct
     4801 ggcctgcttt tcgatctggc gacagggtgg cttatgcaac atcgtattat tctccccgga
     4861 gccactacgc tgacccggtt gatttcagag gtaagggaaa aggcgacgtt gcgcctgtgg
     4921 aacaaactgg cactgatacc gtcagccgaa cagcgttcac agctggagat gctgctgggg
     4981 ccaactgatt gcagccgcct gtctttactg gaatcactga aaaagggccc tgtgaccatc
     5041 agtggtccgg cgtttaatga agcaattgaa cgctggaaaa ctctgaacga ttttggcctg
     5101 catgctgaaa acctgagtac actcccggct gtgcgcctga aaaatctcgc acgttatgct
     5161 ggtatgactt cggtgttcaa tattgccagg atgtcaccgc agaaaaggat ggcggttctg
     5221 gttgcctttg tccttgcatg ggaaacgctg gcgctggatg atgcattgga cgttctggac
     5281 gccatgctgg ccgttatcat ccgtgacgcc agaaagattg ggcagaaaaa acggctccgc
     5341 tcgctgaagg atctggataa atctgcattg gcgctcgcca gcgcatgttc gtacctgctg
     5401 aaagaagaaa caccggacga atcgattcgt gctgaggtgt tcagctacat cccaaggcaa
     5461 aagctggctg aaatcatcac gcttgtccgt gaaattgccc ggccctcaga cgataatttt
     5521 catgaagaaa tggtggagca gtacgggcgc gttcgtcgtt tcctgcccca tctgctgaat
     5581 accgttaaat tttcatccgc acctgccggg gttaccactc tgaatgcctg tgactacctc
     5641 agccgggagt tcaagctcac ggcggcagtt ttttgacgac gcaccaacgg aaattatcag
     5701 tcggtcatgg aaacggctgg tgattaacaa ggaaaaacat atcacccgca ggggatacac
     5761 gctctgcttt ctcagtaaac tgcaggatag tctgaggcgg agggatgtct acgttaccgg
     5821 cagtaaccgg tggggagatc ctcgtgcaag attactacag ggtgctgact ggcaggcaaa
     5881 ccggattaag gtttatcgtt ctttggggca cccgacagac ccgcaggaag caataaaatc
     5941 tctgggtcat cagcttgata gtcgttacag acaggttgct gcacgtcttt gcgaaaatga
     6001 ggctgtcgaa ctcgatgttt ctggcccgaa gccccggttg acaatttctc ccctcgccag
     6061 tcttgatgag ccggacagtc tgaaacgact gagcaaaatg atcagtgatc tactccctcc
     6121 ggtggattta acggagttgc tgctcgaaat taacgcccat accggatttg ctgatgagtt
     6181 tttccatgct agtgaagcca gtgccagagt tgatgatctg cccgtcagca tcagcgccgt
     6241 gctgatggct gaagcctgca atatcggtct ggaaccactg atcagatcaa atgttcctgc
     6301 actgacccga caccggctga actggacaaa agcgaactat ctgcgggctg aaactatcac
     6361 cagcgctaat gccagactgg ttgattttca ggcaacgctg ccactggcac agatatgggg
     6421 tggaggagaa gtggcatctg cagatggaat gcgctttgtt acgccagtca gaacaatcaa
     6481 tgccggaccg aaccgcaaat actttggtaa taacagaggg atcacctggt acaactttgt
     6541 gtccgatcag tattccggct ttcatggcat cgttataccg gggacgctga gggactctat
     6601 ctttgtgctg gaaggccttc tggaacagga gaccgggctg aatccaaccg aaattatgac
     6661 cgatacggca ggtgccagcg atcttgtctt tggccttttc tggctgctgg gataccagtt
     6721 ttctccacgc ctggctgatg ccggtgcttc ggttttctgg cgaatggacc atgatgccga
     6781 ctatggcgtg ctgaatgata ttgccagagg gcaatcagat ccccgaaaaa tagtccttca
     6841 gtgggacgaa atgatccgga ccgcaggctc cctgaagctg ggcaaagtac aggcctcagt
     6901 gctggtccgt tcattgctga aaagtgaacg tccttccgga ctgactcagg caatcattga
     6961 agtggggcgc atcaacaaaa cgctgtatct gcttaattat attgatgatg aagattaccg
     7021 ccggcgcatt ctgacccagc ttaatcgggg agaaagccgt catgccgttg ccagagccat
     7081 ctgtcacggt caaaaaggtg agataagaaa acgatatacc gacggtcagg aagatcagtt
     7141 gggagctctg gggctggtca ctaacgccgt cgtgttatgg aacactattt atatgcaggc
     7201 agctctggat catctccggg cgcagggtga aacactgaat gatgaagata tcgcacgcct
     7261 ctccccgctt tgccacggac atatcaatat gctcggccat tattccttca cgctggcaga
     7321 actggtgacc aaaggacatc tgagaccatt aaaagaggcg tcagaggcag aaaacgttgc
     7381 ttaacgtgag ttttcgttcc actgagcgtc agaccccg
//
