LOCUS       NZ_CP066908            11971 bp    DNA     circular CON 30-JUL-2023
DEFINITION  Klebsiella pneumoniae strain XHKP502 plasmid pXHKP502-4, complete
            sequence.
ACCESSION   NZ_CP066908
VERSION     NZ_CP066908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17173807
            Assembly: GCF_024346815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Zhu,W.
  TITLE     Genome sequence of XHKP502 which was isolated at Aug 10, 2016
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   Zhu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-DEC-2020) Clinical Laboratory, Xinhua Hospital
            Affiliated to Shanghai Jiaotong University School of Medicine, 1665
            Kongjiang Road, Shanghai 200092, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 311.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 03:54:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,904
            CDSs (total)                      :: 5,781
            Genes (coding)                    :: 5,616
            CDSs (with protein)               :: 5,616
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 62 of 165
            Pseudo Genes (incomplete)         :: 108 of 165
            Pseudo Genes (internal stop)      :: 22 of 165
            Pseudo Genes (multiple problems)  :: 27 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066908.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="XHKP502"
                     /isolation_source="Tracheal intubation"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pXHKP502-4"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="31.21 N 121.45 E"
                     /collection_date="2016-08-10"
                     /collected_by="Xinhua Hospital"
     gene            1..1803
                     /locus_tag="JG791_RS29395"
     CDS             1..1803
                     /locus_tag="JG791_RS29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_255074406.1"
                     /translation="MRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARR
                     HVWNYAYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWC
                     AGGLVAGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEP
                     VPVNALADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATN
                     ALIRCVRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYAL
                     KNLLVAGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDV
                     KGLITKLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQI
                     IIARDEFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDL
                     PADFVKTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGD
                     SFIQKEARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTD
                     PLAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1803..2534
                     /gene="mobC"
                     /locus_tag="JG791_RS29400"
     CDS             1803..2534
                     /gene="mobC"
                     /locus_tag="JG791_RS29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2541..3071
                     /locus_tag="JG791_RS29405"
     CDS             2541..3071
                     /locus_tag="JG791_RS29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3098..3277)
                     /locus_tag="JG791_RS29410"
     CDS             complement(3098..3277)
                     /locus_tag="JG791_RS29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3303..3731)
                     /locus_tag="JG791_RS29415"
     CDS             complement(3303..3731)
                     /locus_tag="JG791_RS29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3895..4209)
                     /locus_tag="JG791_RS29420"
     CDS             complement(3895..4209)
                     /locus_tag="JG791_RS29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4197..4475)
                     /locus_tag="JG791_RS29425"
     CDS             complement(4197..4475)
                     /locus_tag="JG791_RS29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4650..5015)
                     /locus_tag="JG791_RS29430"
     CDS             complement(4650..5015)
                     /locus_tag="JG791_RS29430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5012..5383)
                     /gene="tolA"
                     /locus_tag="JG791_RS29435"
     CDS             complement(5012..5383)
                     /gene="tolA"
                     /locus_tag="JG791_RS29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5657..5902)
                     /locus_tag="JG791_RS29440"
     CDS             complement(5657..5902)
                     /locus_tag="JG791_RS29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6547..8034
                     /gene="ltrA"
                     /locus_tag="JG791_RS29445"
     CDS             6547..8034
                     /gene="ltrA"
                     /locus_tag="JG791_RS29445"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            8139..9824
                     /locus_tag="JG791_RS29450"
     CDS             8139..9824
                     /locus_tag="JG791_RS29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9834..10091
                     /locus_tag="JG791_RS29455"
     CDS             9834..10091
                     /locus_tag="JG791_RS29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            10176..10325
                     /locus_tag="JG791_RS29460"
     CDS             10176..10325
                     /locus_tag="JG791_RS29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            11809..11955
                     /locus_tag="JG791_RS29465"
     CDS             11809..11955
                     /locus_tag="JG791_RS29465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_255074402.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYQSGRFFSGYRPVF
                     RSG"
                     /name="hypothetical protein"
     BGC             3139..11971
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(10429..11007)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1804..2531
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgcgttttg tggggctgag ccataaggcg cggcgggagt atgccgatcg ctataagcgc
       61 gataataacc ggactgtgcc attttcggtg ctggcctcgc tggattcacc cgggcggatg
      121 gtcagggcac ggcgacatgt ctggaactac gcgtactggc tgaccggcgc tgccggtctg
      181 gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg
      241 cgcccggacc tctatgcgaa cgcagattat tacctgctgc agccggctaa cctgttcggg
      301 atagcctggt gtgccggagg gcttgtcgcc ggcgtccttc tggcgcggct gctggcgttc
      361 ctgctgcttg atggtcattt tgcggccgct gacgaggcgg tgaacgcgaa actgaatcag
      421 gagagccggc gcagcagcca gcggaccgga gaaatgacag acgtccgcca cctgcatttc
      481 ggggagccgg taccggttaa cgcgctggcc gatttttcca cggagcaggc ccggaaacag
      541 caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc tcgtgatacc
      601 tggcgcaaga ccaatattca gatcctcggc ctgcccggga gcggcaagag cgcgatggcc
      661 accaacgcgc tcattcgctg cgtacgcgat ttcggggacg ctgtggtgta cttcgacccc
      721 aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt caccctgctg
      781 gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga ccagtacgcc
      841 ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac
      901 taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagttccc gcagggggca
      961 aacattcagc aggtgctggc ggcggcgtac gcgttgccgg aggccctgaa aaaggacgtg
     1021 aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtcctgca gacggacagc
     1081 ggtatcgatg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg
     1141 gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc
     1201 attgcgcggg atgagttccg gaggtggccg cacgcgagca tcatgctcga tgagattaaa
     1261 tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagccg ggactgcaac
     1321 ctgctgctcg cgcaccagtc gctgggcgac ttcggtcagt gcgggcagga tctgcccgct
     1381 gattttgtga aaaccacggt actggataac acgccgatcc gctggttcta tcgcgcggcc
     1441 agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg agatccgggt ggacgtggag
     1501 cgccgccggg ccagccggga ggcggggaac gtggagcaca tcagcggcga cagcttcatc
     1561 cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacacctgcc tgacgggttc
     1621 gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca gcagcccgct gcgggttgag
     1681 cgccgggaaa tttcgctgaa aaccttcccg gtgctggcga aaacggaccc gctggcggag
     1741 tacctgcctc cggccgacgg cggcggacca aaagcggatg atgattttgc ggggctttac
     1801 tgatgacact ggagcagtac aacgtcagcc acgcgaagcg gcaggcgcgg aacgcggaga
     1861 aaacacggct gaccctgcgc tggctgcggg aagagctgtg ttccacggcg gagctggttg
     1921 cgcgacggct ggggattgcg gcggtacagc cggtttaccg cttcctggac agtctggtgg
     1981 cgaaagggct gctggtccgg gcgaaatacc cggttgacgg ccgtcaggtc agcgtgtggg
     2041 ggctgacgcc gcacggggtg gcgttctcgt tcgacgagga cgagccgctg acggacgtta
     2101 ttccgtttca gccgtcaagg gtgtcggcgg cgcagctgcc gcaccggctg gccgtgcagt
     2161 ccctgcgcct ctccatggag ggccgggggg ccaccggctg gcggtacctg caccgtatgg
     2221 ccctgaaagg gatgaaagtg ccggacgcgc tggcggagct ggacggcaga acggtggcat
     2281 ttgaggtaga gcgcaccgtc aagagccgca ggcggtatca ggaagtggtg tccgggtatc
     2341 tgttcaaccg ccgggcgaac ggcatcgatg aaatctggta catctgccct gaccggccga
     2401 cgcttgtccg ggtacagcgg gcgattctgg cggtggatga aatcgtgaat ccacagaccg
     2461 gcgaggcccg caaaacggtg gagctggacc gggagaggct gttcgcgtgc tttaaattta
     2521 tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaggac gtggaagaca
     2581 aagacggcga gcgctgcatt atgccaacca gcgagcatct ggaagacatg gtggtgctcg
     2641 cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga
     2701 tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc acgtactttt
     2761 tcaccggtga aaatccgcaa tgggaacggg acaaacccac aggaatgaag ctgtgtgatt
     2821 acctctatcg aaaggtggag cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg
     2881 ggaagtgctc accggagcag attaacgcga tgattgaggt ggtcagcgag cggcacgggg
     2941 aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac agccgctatt
     3001 ttaacgtgcc ggtgcctgaa ctgccggagg acaaacccgc ccgccggtgg tggccgttcg
     3061 gcaaaaaata acgccagcac cggcatggcc ggtgctgtta ttcttccccg agcttatccc
     3121 gcatcacctg ccagagtgtt tccgcatcct cgtgaagccg ttcgcacatg tcggccgggg
     3181 tatccatgtc cagctcgttc agcttttcca gcaacagcag cgactgatcc ttaatgaatt
     3241 tagccatatt gagggcagtt ttttcctgct tcgtcatgtt ctccattccc ttcggtaagt
     3301 tctcagctaa accagccgga ccagaagcgt tttttcttct gtgtcagttc tgttgtcaat
     3361 ctgtccactt cggcctggag tttccggcgc tcatcatcgt gctggcggcg ttcttcttcc
     3421 cgggatgttt tatcttccag catcagggtg acagcctgcc ggagatcggc cagttccttt
     3481 tgcattgcca gaagctgttc ctgtgggaaa tttttttgtg tattttcacg gtgtgaaatg
     3541 ccgggaattt gtttctcagg gatattcaca tttccgtata cacggatcag ttctgacaca
     3601 tccacgacag ggttgttttt cccgtcacgt gacacggtaa cctttccctg ttttacatga
     3661 ttgtatagcg ttcttctggt aatgcccgcg gcttgtgctg cctgtgaaag gttgagcaat
     3721 gttttcgcca ctgtataccc ctgattttat cgggtgtgaa aggtgtgtaa tttctcacgg
     3781 taaatgtatc gctgaatttt acccgttttt ccgttcagga tgtggttttc tgacgcgctg
     3841 cgcttgtcaa accggcgacg gccacgcaat gaattgcgcc cccgtcatcg gttattagag
     3901 gctgtctgcc cccggatacc cggattcttt ctgatgcctg acggtgacga tcacaatctg
     3961 gtccatttcc cggtcgtggc ggtagagcat cacatagcca ctgtcgccaa acccgatcac
     4021 cagttcctta tattccagcg gcagaaacgg caccgggcgg ccaatgtctg gcaatgtttt
     4081 cagttgctgg atagcccgga cgatcacctc acctcctttt ctggctgcca gccgattttt
     4141 ggttttgaga aagtcctgaa ggcgttgtaa atcgcgctgt gccagcgcgg aaatcgttac
     4201 ctgtggcatg gcggagcatc ctgctcgttc tcagttcccc aggtacttat ccaggcttcc
     4261 gcttcttccg cagtaaggtg cagacccgtt tcctgatagt gctgccatgc ggcctgtccg
     4321 tcccgcaggt actggctgcg cttctcttcc cggtcgatgt attcggtgat tgcctccagc
     4381 attagcgcgt gcgctgagcg gtgccggtca tcggccagcg ttttcagccg gtccttaagt
     4441 tcttcatcga gcctgatgga tgttgctgat gacataaaag cccctttgta gtcatatgta
     4501 ttacacatga ctactttaat ccgtggcggc gctatttgcc agaaatacgg cttttgctgc
     4561 ccatttttca gcttcgcttc cactaatcag ggctgctaca ggctcactca tcatccagga
     4621 ctattgcagc ctgcgggtta aaggacctgt tactggcggc aaccatcatc attgagcttt
     4681 tgggtggtga ctggtcgcgg atcctgtaag ccgtcatttt tcagactgaa ttcaaaattt
     4741 ataacctgtc gcattgccgg gtgccctgcg ggatatagcc agtgcctcat tgccgtcagc
     4801 gtttcccgaa aaaaaaggtg acagccggta agcgacacgt tgtccactcg tccatcgtcc
     4861 agaatatcaa acgtggcggt cacctgacct tcgatgcgtt ttgctgcggc ctgtaccggg
     4921 taggttggct gcagtgtggt tacgggtttt agtggtatcg catgtaccgg agtgaggaac
     4981 atggccgcca gcactgtcag agtaaaagct ttcatggtct gaaatccaga ggggcgtttt
     5041 tgaatatttg ccaggtattt tcatccggtg ccggcggaat tttggcgtgg gtaacggctg
     5101 atattgctgc tttacagagt ttcggatctc ctccttcagc aatggcgctg ttcagagtgc
     5161 catcacgttg tatggatatc cgcacggtgc acatttttcc cgcataggcc tctggttcaa
     5221 acatctgcat ttcaacagcg gtcttgatgg ccgcagcgta gctactgatt ttagatgact
     5281 tatccagagt ataaattttc ggcggctggg tggtggaatg cttacaggcc gtcaggagta
     5341 aggggctgca caatgccagc aggataaagg gtattaaacg catcgggttg atgacctgtc
     5401 agtaaaaaat gttacatgta tatttgtcac tctttacggc cacgttcatt tacataaaca
     5461 cggcttatcg tatcgttttt acaccaaacg gcccgccatg cagttgcgtg cataggcatg
     5521 cagcgggtta caatttaccg ggcgcgtttc cggcggtttt ccgggtggtt tgttgcctgt
     5581 tttatcccgt agccgccgga aacgccctca gaccgtctga gcggtgcggg caatgcgacg
     5641 ttatggtaaa taccccttac cggcggcgct gtggctgcca ggtgaaggtg gtttccgtag
     5701 cctcgtattt ctcccgacgc atttcctgcg cccgcctggc ttcacgttcg tcctgaatgg
     5761 cacgaagcac cctttcccgc acctgttgtt ttaccgcctt tggcagcgtt ttgttccctt
     5821 tcaggcccat ggccagtgcc tgagcgacaa aggcgtccgc caggcgcttt tcttcagcgg
     5881 gagtcagttt caggtgcgtc atagtggcct ccgggcgtct caggtttccc gaacagttta
     5941 accgcaggtc ctctcctcat gccagttagc tttcagatgt gtctatacca tctgaaatgt
     6001 aagtgtgatg aatgtcttaa ataataaaat ttgttgccat gagaaatttt cgggaaaaga
     6061 cgcgatctgc ttacaggtgg ctccgggcgc ttttttgtgc ctgaagatga gaactaatct
     6121 catatattga cacctgaaaa cgggaggagt aaggtaataa ttatactgtg tatatgtaca
     6181 gtgtgcgccg ggagaccggt tagagatcaa ggggtgaaag tccccgacca ttgaaggacc
     6241 agcaatccac aaggtccccg agtcatgcgt tgcataccgc gaggtatggg gcgaagcgtt
     6301 gacaggggtg ttgacaggcc agccattgag ccacgaaatg tatattaaat taccgggtgc
     6361 cgacgttgta ctgttaacgg aaggcaacat catagggtgc gacactgcga gtgccacatg
     6421 gacccggcgg ggtctgagac cctggcatgt caatacgatc tctacgcggg aaccgggaga
     6481 tctcccctct gaccatctgc cagtgtcgga gatggcccgc accgggaaga cgaggagtca
     6541 tagccggtga tgtacggaga ggagaagtcg gactcgctca tagtagcggc gaagcaggcg
     6601 aacaacccga aaggagcgga gtcagtggag cgaaggagcg gggccaaggg gaacgcggaa
     6661 cagccacaca tgcgccggac acagagccgg gaaagcatgt cacagaggct gtcacgcgtg
     6721 cgggaagctg cgaagcagcg gaagaaagaa cggtttacag cattgttcca cctgctgaca
     6781 gtcgaagcac tggaagccgc attcctctcc ctgagcagga aagcggccgc cggagtggat
     6841 ggcatcaggt ggatggacta cgccggaaac atgaagaaca acataacaga tctgcaccgg
     6901 aggctacatc agggcagcta cagggcgcag cccggcaggc gtcactacat cccaaaagcg
     6961 gatggaaaac aacgcccgct cggcatcgcc tcgctggagg acaagatcgt ccagtatgcg
     7021 ctggtgaaaa tcctgaacgc agtctatgaa aacgacttta tggggttctc atacgggttc
     7081 agacccgggc gaagccagca cgatgcactg gacgcactgg ccacagggct ggtacgcact
     7141 aacgtaaact gggtactgga tgccgacatc agtcagttct tcgacagggt gagccacgaa
     7201 tggctgatca ggttcacaga gcatcggatc ggcgaccgga gggtaatcag gctcatacgt
     7261 aagtggctca cagccgggac gtcggaggag ggtcaatggc gagcaacgga ggaaggcacc
     7321 ccacagggtg cggtcatctc accgctgctg gcaaacatat acctccacta cgtcttcgat
     7381 ctgtgggcgc atcagtggcg acgtcgctat gccacaggca atgtggtaat ggtcagatac
     7441 gccgatgaca tcgtcatcgg gttcgacaaa cgatacgatg cccggcgctt ccgtatagcc
     7501 atgcagcgca gactgaggga gttcggactc acggttcacc cggagaaaac ccgtctgatg
     7561 gagttcggcc gcttcgctgc cgaaaaccgt gccatcaggg gaaaaggcaa accagaaacg
     7621 ttcaacttcc tcgggttcac gcacatcagc gggaaagatc gcaacggcag gttcatgctg
     7681 atacgaaaga cccgccggga tcggatgacg gcaactctga aagccatcaa agacggtctg
     7741 cgaaggcgct ggcattactc aatccccgaa cagggaaaat ggctcaggag agtggttcag
     7801 ggatacctga actatcactc ggtaccgggc aacttcccca ccatgcagaa gttcaggaca
     7861 cacgtaacaa acctctggcg ccgggcgctc aggcgcagga gccagaagga tgatacgacc
     7921 tggacgaaag caaacaaact ggcagccgca tggctaccaa gggttcgggt tcttcatcca
     7981 tggcctgtgg agcggttcac cgccagacac ccgaggcagg agcccggtgc gtaaatcgcg
     8041 cacgccggga tctgtgcggg gggtatccgg taacgggtat ccctaccgcg acatgttggc
     8101 tttaatttat tgttttaaat gtcaaagagg aaaacggtat gagtggtgga gacggtcgag
     8161 gtccgggtaa ttcaggtctg ggacataatg gtggtcaggc cagtgggaat gtgaacggta
     8221 cgtctggtaa aggtggccct tcatcaggtg gtggtacgga tccaaacagc gggccgggct
     8281 ggggtacgac gcatacgccg aatggagata ttcataacta taatccgggg gagtttggta
     8341 acggcgggag taaacccggt ggtaatggtg gtaacagcgg caatcatagc ggtagctctg
     8401 gtggcggaca gtcttcggcc actgcgatgg ccttcggtct gcctgccctg gctactccgg
     8461 gggctgaagg actggcttta tcagtttccg gcgatgcgtt gtcggccgcc gttgctgatg
     8521 tgctggctgc cctgaagggg ccgtttaagt ttggtctgtg ggggattgcg atctacggtg
     8581 tgctgccttc tgagattgca aaagatgatc cgaaaatgat gtcaaaaatt atgacgtcat
     8641 taccggccga tgcggtgacg gagactccgg caagtacttt accactggac caggcgacgg
     8701 ttcgtgtcag acaacgggtt gtggatgtgg tgaaggatga gcggcagcat attgcggttg
     8761 tcgcaggtcg gccaatgagt gtccctgtgg tggatgcgaa accgacaaaa cgtccggggg
     8821 tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt gagcgtaccg aaaggtgttc
     8881 cgacagcgaa agccccgcca aaaggcattg ttgctgaaaa aggtgattca cgtccggctg
     8941 gttttacagc cggtggtaac tcccgtgagg ccgttattcg tttcccgaaa gagaccggac
     9001 agaagccggt ttatgtgtcg gtgacagatg ttcttacccc ggcacaggta aaacagcgtc
     9061 aggaggaaga aaagcgtcgc cagcaggcat gggacgccgc tcatccggaa gaggggctga
     9121 aaagagacta tgataaagcg aaagctgagc tggatgccga agataaaaat attgcgacct
     9181 taaacagccg cattgcatcg acagagaagg cgctccccgg tgcaagggct gctgttcagg
     9241 aagccgataa aaaggtaaaa gaggcagagg caaataaaga tgattttgtg acttataacc
     9301 cgcctcatga atatggctcc gggtggcagg atcaggttcg ctatcttgat aaggatattc
     9361 agaatcagaa tgagaaatta aaagcggctc aggcatcttt aaacgcaatg aatgaatcct
     9421 tatccagaga taaggctgca cttaccgggg cgatggagag ccggaaacaa aaggagaaaa
     9481 aagcgaagga tgcagaaaat aagttaaatg aggaaaagaa caagcctcgc aaggggacta
     9541 aagactacgg ccatgattat catccagtac ctaaaacaga agacataaag ggacttggag
     9601 agttaaagcg aggtgatcct aaaacaccta aacaaggtgg tggtggtaag cgggcccgat
     9661 ggtatggcga taaaaaacgt aaaatttatg aatgggattc ccagcacggt gagcttgaag
     9721 ggtatcgtgc cagtgatggc gaacacctag gcgcatttga tcccaaaacg ggtaagcagg
     9781 ttaaagggcc agatccaaaa cggaacatta aaaaatatct ttgagaggta aatatggggc
     9841 ttaaattacg tttgacatgg tttgataaga aaactgaaga gtttaaaggg gaagagtatt
     9901 ctaaagactt tggtgacgat ggttctgtca ttgaaagctt gggaatgcct ttaaaggata
     9961 atattaataa tggtgagttt gatttgaaga aagaatgggt tcctttacta cagccatttt
    10021 ttaaaaatca aataaaacct gatgtggata attgttatat agcttttgat tatcgggacg
    10081 gtaactggta acaaaaaatt atcaaggatg atttgaaata ctgacttccg gtgttcaggg
    10141 atgaatgccg gagtctttaa atacagggaa agtatatgaa aaaggtaaag actatttttc
    10201 tttttatatt gattgttgct ggctttcttc tggccgcatg tcaggcaaat tatatccggg
    10261 atgttcaggg cggaacggtg gcaccatcgt cctcctctga actgacgggg atcgcggttc
    10321 agtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc
    10381 tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca gttggggaac
    10441 tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc cttccagtgt
    10501 agccggactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca aatcctctgc
    10561 gaactcccag ttaccaatgg ctgctgccag tggcgttttg cgtgctttcc cgggttggac
    10621 tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacggggag ttcttgctta
    10681 cagcccagct tggagcgaac gacctacacc gagccgagat accagtgtgt gagctatgag
    10741 aaagcgccac acttcccgca agggagaacg gcggacaggt atccggtaaa cggcagggtc
    10801 ggaacaggag agcgcaagag ggagcgacct accggaaacg gtggggatct ttaagtcctg
    10861 tcgggtttcg cccgtactgt cagattcatg gttgagcctc acggctccca cagatgcacc
    10921 gaaaaagcgt ctgtttatgt gaactctggc aggagggcgg agcctatgga aaaacgccac
    10981 cggcgcggcc ctgccgtttt gcctcacatg ttagtcccct gcttatccac ggaatctgtg
    11041 ggtaactttg tatgtgtccg cagcgcccgc cgcagtctca cgcccggagc gtagcgaccg
    11101 agtgagcgag gaggcgcaat ttccccggca cctgcaagac ggtcaccgga cgcgctgatg
    11161 tgggatattg tcccctgatt aagtctttct attccctgac gatcaattat caggataggt
    11221 gaaccatgtc ggtttttcgc tgaacatttt acgttaacct atctctgttg cttttttctc
    11281 cttgcggaga aatgcaagtc atccccgctg gggcgtggct gacttcaggg gaaaagacct
    11341 tgcttacgct ggctggacgc caccgacgca cggtcttttc ccctctttct ggctctgtcc
    11401 ggctggacgc cggccacgcc agaaattcac ccggcccccg tcaggctcac gcctgaccac
    11461 ccacaaaaat ccacggtgct ggacgcacct gacgaagcag caaagctgct cctgattttg
    11521 cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc ggaaaagacc
    11581 gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt cctccggggc
    11641 tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg cggtgaaagc tgaacgcaat
    11701 caccacggac gtgaccgccg ccgccatgcc ttggggcatg tccgcggctt gtgcagcggg
    11761 ggctgcgcat cacgccggta aacccgtcgt ctgaaggaga gagattgcat gtttaatacg
    11821 gattcgcttg cctggcagtg gcatatcccg gagctggccc ggcagattta cccgtgggtc
    11881 agctggctgt accgggagta ttaccagtct ggccggtttt tttctggtta tcggcctgtt
    11941 tttcggtctg gctgaactct gggccggccg a
//
