LOCUS       NZ_CP066903             5596 bp    DNA     circular CON 30-JUL-2023
DEFINITION  Klebsiella pneumoniae strain XHKP309 plasmid pXHKP309-3, complete
            sequence.
ACCESSION   NZ_CP066903
VERSION     NZ_CP066903.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17173631
            Assembly: GCF_024346775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhu,W.
  TITLE     Genome sequence of XHKP309 which was isolated at Dec 14, 2015
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2020) Clinical Laboratory, Xinhua Hospital
            Affiliated to Shanghai Jiaotong University School of Medicine, 1665
            Kongjiang Road, Shanghai 200092, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 285.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 04:02:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 50 of 126
            Pseudo Genes (incomplete)         :: 76 of 126
            Pseudo Genes (internal stop)      :: 16 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066903.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="XHKP309"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pXHKP309-3"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="31.21 N 121.45 E"
                     /collection_date="2015-12-14"
                     /collected_by="Xinhua Hospital"
     gene            1..177
                     /locus_tag="JG788_RS28015"
     CDS             1..177
                     /locus_tag="JG788_RS28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            167..511
                     /locus_tag="JG788_RS28020"
     CDS             167..511
                     /locus_tag="JG788_RS28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            720..>1154
                     /locus_tag="JG788_RS28025"
                     /pseudo=""
     CDS             720..>1154
                     /locus_tag="JG788_RS28025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1189..1698
                     /locus_tag="JG788_RS28030"
     CDS             1189..1698
                     /locus_tag="JG788_RS28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1705..1917
                     /locus_tag="JG788_RS28035"
     CDS             1705..1917
                     /locus_tag="JG788_RS28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2584..2808
                     /locus_tag="JG788_RS28040"
     CDS             2584..2808
                     /locus_tag="JG788_RS28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2792..3058
                     /locus_tag="JG788_RS28045"
     CDS             2792..3058
                     /locus_tag="JG788_RS28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3128..3478)
                     /locus_tag="JG788_RS28050"
     CDS             complement(3128..3478)
                     /locus_tag="JG788_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3544..4089)
                     /locus_tag="JG788_RS28055"
     CDS             complement(3544..4089)
                     /locus_tag="JG788_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4115..4441)
                     /locus_tag="JG788_RS28060"
     CDS             complement(4115..4441)
                     /locus_tag="JG788_RS28060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          5432..5511
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4849..5246)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          502..2033
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa gatcctggca tccggcagtg
       61 atggcaaatc tgatgcgtca ggcacaggag gaagattacc gccgggagca gatggcagag
      121 cttgagcgtt cagcccggtc acattttgcg gatatcggag gagaggatgg cggttaagcg
      181 tacccgtttt ttaggtatcc gggtgaccga tggggagtac cagcagctgc tggagcgctg
      241 caatggaagg caactggcgg tgtggatgcg ggaaacctgt ctcgatacgc gcccggcgcg
      301 gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc cagcttgccg ggatggggaa
      361 caacctcaac cagatcgccc ggaaaattaa cggcggccag tggtcaggcg ctgaccgggt
      421 tcaggtggtg gccgcgctga tggccattga tgccgaactc gagcggctgc ggcatgccgt
      481 gctggaaaag ggggcagacg atgatcgtta agtttcatcc gcgagggcga ggcggcggtg
      541 ccgggccggt ggattatctg ctggggaaag accgccagcg cgacggggcc atcgtcctgc
      601 agggaaagcc ggaggaggtc cgggagctta tcgacgcctc gccctacgcc aaaaagtaca
      661 cctccggcgt cctgtctttt gctgaacagg atttaccgcc cggccagcgt gaaaagctga
      721 tggcgagctt cgagcgggtt ctgatgcccg gactcgataa agaccagtac agcgtgctgt
      781 gggttgaaca ccgggacaag gggcggctgg aactgaattt tctggtaccg aacacggaac
      841 tgctgaccgg caggcgtctc cagccgtact acgaccgggc agaccgtccg cgcatcgatg
      901 cgtggcagac cgtggtgaac gggcgactgg ggctgcacga cccgaacgca ccggagaacc
      961 ggcgcgcgct ggtgacgcca tcttcgttac ccaaaacgaa gcaggaagcc gcagaggcta
     1021 ttacgcgggg cttactggcc ctcgcctcgt caggggagct taaaacgcgt caggacgtca
     1081 ctgaggcgct ggaaagcgca ggttttgagg tcgtgcgcac cacaaagagc agcatcagca
     1141 ttgccgaccc ggacggaggg cgaaacatcc gacttaaggg agccatctat gaacagtctt
     1201 ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagag taccggcgaa
     1261 atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact gagcgaaagc
     1321 gggaagagaa tcagcgccgc catccgcgcc cacgagcagg gtatgagcgc ggccatgcag
     1381 tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat ggctgaccat cacgatggtg
     1441 tcaggactgc tgttcgccag cctgagcggg gtgttgtggt atcaggggag cttgatagcg
     1501 tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt cgaagctgaa cgcgaagacc
     1561 tggggcgtga cgtacctgga agacagcaac ggacgttttc tggtgctgcc gaaggggacg
     1621 gccgtagaca ggacgcagag ctggacggtc gggaacgggc ggtcgaagca gaacgcgctg
     1681 agactggtga aggagtaagt gccgatgacc gagctggaaa aacagttgct gagcgcatta
     1741 gagcagctac agcaggacta ctcgaaaagg ctggacgagt gggagagcgc cttcggggaa
     1801 tggcggaaaa tgtcagggct tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgacg
     1861 cgcttgagtc agcaggtgga acgcttgagc gggcaactgc agcgtttgag ccggtagtgc
     1921 agcgtcatga gatggccgtg gccgcagaac gggcgcacga gcaacggcag cacgaaaagg
     1981 aactgacgga agcgcgttcc cggcagcgaa gctatgacgg tccgtcgctg ggctgaaatg
     2041 caggaaaaca gggttctcat cgggcattta cgctgaaacc ggatgtcatt cacctggctg
     2101 catggctatg cagccaggta aaatttatct gtcgcgaacg gggcggttta tcgggtggtt
     2161 tgttgccggt tttacctgac tgccaccccg ttcgcggcga acccgtccgg ggcggtgcgg
     2221 gcaacgggtg ttatgtttaa tcagcgtgaa cggcagatac aaaaaagccc cggagggctt
     2281 ttaactactg acttattttt ttgaagattt gctgggtttc aatttgtcag tatttcccgg
     2341 cgttttttta ttgtcacgct ggcgtttatc aggttcccct gttgaatcgg caattcgttt
     2401 cggacccggc ttaagaccca taatcgttat cctttcaata gttaagagga aagccctcaa
     2461 gctagatata ttgaccgcgt tacggattat caagaggggc taacaagatt attttttgtt
     2521 cttttaggtt gattgtggta ctgttgatat acatgtatag caaatggtgt tcgggaggtt
     2581 tatatgcttg ctatccggtt atctgatgag attgagtccc gtctggactc gctggcgaag
     2641 caaaccggca gaacaaagac gttttatgcg cgggaagcaa tactggcgca tctggaagac
     2701 ctggaggatt attatctttc agcagaaact gctgcacgcg ttcgccgtgg tgatgaagca
     2761 gtgcattcgt ctgaagacgt gaggaagtca cttggtctgg acgattaact attccgatcg
     2821 ggcgctcaaa tcgttacgca agatggacaa acagaacgca cgacggattg tggattttat
     2881 gagtttacgc attgcagttg ctgccgatcc tcgccagtca gggaagccgc tcaaaggtga
     2941 gctgggcgag ttctggcgct atcgggtggg agattatcgc gttctgtgtg agatccgaga
     3001 tgacgagctt gttatccttg ccgccacgat tggacatcgc cgcgaagttt acgactgaag
     3061 cccgctatgc gggctttttt gatgccgaca acaggtatta tggtaaatac tcgactaact
     3121 acgtgttcta ctgtttgtcg gttttagtta accagcaggt tgtcttaacg ataatgtagc
     3181 cgttattttt aactaataag gtcatattgt taacgtcagg cataataaaa gacattatgg
     3241 cctcagagtt atcatcagaa gcagctctct gcattactac ttcgattccc ttttctttga
     3301 atctataagt atccagttgc ctattaaact tagaaagacc tacaaccata cttagtttgt
     3361 tatctttaac agtaagagat gcttcctgag taccattaca tcggtagtaa gcggtgtaac
     3421 cgtcagcaaa agatcgaccg gggaatataa atgctaggaa cgcaattaac caatacatag
     3481 ttgagaatct tggtattaca catttcacta tcctctcttt catttttcgt tcaccatact
     3541 ttgttatctg atttttaatt tttctatttt ataaaccaag tctctaccaa aaaggcgaaa
     3601 attatcctca aataataata attgtttaag aacatccatg aaagcagctg cttcaccctc
     3661 agttatggga gctactctat ataacatata acctgagtta agggtttctt ttatactttc
     3721 ttcaatgctc attataaata ttagtggatt atcatttttc ttcccccatg ggtttctaat
     3781 taatccttcg atgtactcag gtcctttgcg tcctattgcc tgcgcaatat tttttatttc
     3841 atctgcgtga gggctttgtt gtacagcttt gtttatgcct gcttgagtga ttttcagaga
     3901 ctctacaccg tgtatgttgt atgagataaa atgtaataaa ttaagatgca atgcaattaa
     3961 tccatttttg tttacatctg agtatttgga taaaaaacgt aatgaagtgt taactctccc
     4021 ttgaagccta acaattggga gtgctgcctt gtatatttcc ttctccgtta aataccgttt
     4081 aaaaaacata attctccgcc tccgtatagc gtgcctattt tatttcgtcg taaaacttag
     4141 ctctgaaata ttcctttaga accatcatgt cattaataat tgaatctatc tcatccattt
     4201 tttcttggta ttcttttaat ggtgaaggga gcggtaactc aatatttgaa aagtgattgg
     4261 cgagataggg atcaaggttt ctcatccatt caggcttttc gccaatgcgt tcttcaactg
     4321 aaagtgaacc tgataatctc tggttaataa gatcacttag actcattttg gttttattcg
     4381 aaagccgttg taaggattgc tttaatccta tatctagcat gatatttgtt ctttctctca
     4441 ttaccccccc ttgctattgc gttttttgtt gcgcacaaag ctaccattgc gcattaagaa
     4501 acgcaataaa aaaggaggga gggagtggtt tgtacatgat gtgcagtgcg ctgagggcat
     4561 cagtggcggg tgtttttccg tgggatggat ggagctccgg ggataacaga actgacgatc
     4621 atttaaagga tcttcttgag atcctttttt tctgcgcgta atctcttgca ctgtaaacga
     4681 aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc agttggggaa ctgcttaacc
     4741 gggtaactgg gtatacgaga ccgcagatac caaaactgtt ctttcagtgt agccgcagtt
     4801 aggcacccac ttcaagacct ctcaatatct ctctcgtata tatccagtta ccaatggctg
     4861 ctgccagtgg cgtttttgcg tgtccgtccg ggttggactc aagatgatag ttaccggaaa
     4921 gggcgcagca gtcgggctga acggggggtt cgtgcataca gcccagcttg gagcgaacga
     4981 cctaaaccga gccgagatac cagcagcgtg agctatgaga aagcgccacg cttcccgaaa
     5041 gggaaaaagg cggaacaggt atccggtaaa cggcagggtc ggaacaggag agcgcacgag
     5101 ggagccgccg gagagaaatc accggcatct ttaagtcctg tcgggtttcg tcctcctctg
     5161 acttgagcgt ccatttctgt gatgctcgtc aggggaggcg gagcctatgg aaaaacggca
     5221 acgccgcatc cttttctccg gccctttctg atttgagcgc ctttgcttta tcccgttatc
     5281 cgtgagtcgg tcacgcccgc cgcagccgaa cggcaggagc gcagctaccg agtgagcgag
     5341 gaggcgtcat ttcatctgtc cgtgcatggt attgcggacg ctcctgagcc acatatcaca
     5401 cctgccacat gaggcacttt catgaatcgt gctctcatgc caacatagta agccagtata
     5461 cacagcgtaa acgctgtgac tggttcaggg ctgcgccccg aaacccgcta acaccactga
     5521 cgcgctggcg cttgtccaac gccggagcga cccggaaaac ttttccgtgc cgtccggcgt
     5581 ttgcgaagga gggata
//
