LOCUS       NZ_CP066898            10060 bp    DNA     circular CON 31-JUL-2023
DEFINITION  Klebsiella pneumoniae strain XHKP75 plasmid pXHKP75-3, complete
            sequence.
ACCESSION   NZ_CP066898
VERSION     NZ_CP066898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17173614
            Assembly: GCF_024346735.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Zhu,W.
  TITLE     Genome sequence of XHKP75 which was isolated at July 29, 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Zhu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2020) Clinical Laboratory, Xinhua Hospital
            Affiliated to Shanghai Jiaotong University School of Medicine, 1665
            Kongjiang Road, Shanghai 200092, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 383.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/31/2023 10:58:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,878
            CDSs (total)                      :: 5,756
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 57 of 144
            Pseudo Genes (incomplete)         :: 91 of 144
            Pseudo Genes (internal stop)      :: 17 of 144
            Pseudo Genes (multiple problems)  :: 21 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066898.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="XHKP75"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pXHKP75-3"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="31.21 N 121.45 E"
                     /collection_date="2013-07-29"
                     /collected_by="Xinhua Hospital"
     gene            1..246
                     /locus_tag="JG785_RS29275"
     CDS             1..246
                     /locus_tag="JG785_RS29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            520..891
                     /gene="tolA"
                     /locus_tag="JG785_RS29280"
     CDS             520..891
                     /gene="tolA"
                     /locus_tag="JG785_RS29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            888..1253
                     /locus_tag="JG785_RS29285"
     CDS             888..1253
                     /locus_tag="JG785_RS29285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1428..1706
                     /locus_tag="JG785_RS29290"
     CDS             1428..1706
                     /locus_tag="JG785_RS29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1694..2008
                     /locus_tag="JG785_RS29295"
     CDS             1694..2008
                     /locus_tag="JG785_RS29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2172..2600
                     /locus_tag="JG785_RS29300"
     CDS             2172..2600
                     /locus_tag="JG785_RS29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2626..2805
                     /locus_tag="JG785_RS29305"
     CDS             2626..2805
                     /locus_tag="JG785_RS29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2832..3362)
                     /locus_tag="JG785_RS29310"
     CDS             complement(2832..3362)
                     /locus_tag="JG785_RS29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3369..4100)
                     /gene="mobC"
                     /locus_tag="JG785_RS29315"
     CDS             complement(3369..4100)
                     /gene="mobC"
                     /locus_tag="JG785_RS29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4100..6064)
                     /locus_tag="JG785_RS29320"
     CDS             complement(4100..6064)
                     /locus_tag="JG785_RS29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7548..7697)
                     /locus_tag="JG785_RS29325"
     CDS             complement(7548..7697)
                     /locus_tag="JG785_RS29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7782..8039)
                     /locus_tag="JG785_RS29330"
     CDS             complement(7782..8039)
                     /locus_tag="JG785_RS29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8049..9734)
                     /locus_tag="JG785_RS29335"
     CDS             complement(8049..9734)
                     /locus_tag="JG785_RS29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3049..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6867..7445
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3373..4100)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct
       61 caggcactgg ccatgggcct gaaagggaac aaaacgctgc caaaggcggt aaaacaacag
      121 gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa
      181 atgcgtcggg agaaatacga ggctacggaa accaccttca cctggcagcc acagcgccgc
      241 cggtaagggg tatttaccat aacgtcgcat tgcccgcacc gctcagacgg tctgagggcg
      301 tttccggcgg ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg
      361 cccggtaaat tgtaacccgc tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt
      421 gtaaaaacga tacgataagc cgtgtttatg taaatgaacg tggccgtaaa gagtgacaaa
      481 tatacatgta acatttttta ctgacaggtc atcaacccga tgcgtttaat accctttatc
      541 ctgctggcat tgtgcagccc cttactcctg acggcctgta agcattccac cacccagccg
      601 ccgaaaattt atactctgga taagtcatct aaaatcagta gctacgctgc ggccatcaag
      661 accgctgttg aaatgcagat gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg
      721 cggatatcca tacaacgtga tggcactctg aacagcgcca ttgctgaagg aggagatccg
      781 aaactctgta aagcagcaat atcagccgtt acccacgcca aaattccgcc ggcaccggat
      841 gaaaatacct ggcaaatatt caaaaacgcc cctctggatt tcagaccatg aaagctttta
      901 ctctgacagt gctggcggcc atgttcctca ctccggtaca tgcgatacca ctaaaacccg
      961 taaccacact gcagccaacc tacccggtac aggccgcagc aaaacgcatc gaaggtcagg
     1021 tgaccgccac gtttgatatt ctggacgatg gacgagtgga caacgtgtcg cttaccggct
     1081 gtcacctttt ttttcgggaa acgctgacgg caatgaggca ctggctatat cccgcagggc
     1141 acccggcaat gcgacaggtt ataaattttg aattcagtct gaaaaatgac ggcttacagg
     1201 atccgcgacc agtcaccacc caaaagctca atgatgatgg ttgccgccag taacaggtcc
     1261 tttaacccgc aggctgcaat agtcctggat gatgagtgag cctgtagcag ccctgattag
     1321 tggaagcgaa gctgaaaaat gggcagcaaa agccgtattt ctggcaaata gcgccgccac
     1381 ggattaaagt agtcatgtgt aatacatatg actacaaagg ggcttttatg tcatcagcaa
     1441 catccatcag gctcgatgaa gaacttaagg accggctgaa aacgctggcc gatgaccggc
     1501 accgctcagc gcacgcgcta atgctggagg caatcaccga atacatcgac cgggaagaga
     1561 agcgcagcca gtacctgcgg gacggacagg ccgcatggca gcactatcag gaaacgggtc
     1621 tgcaccttac tgcggaagaa gcggaagcct ggataagtac ctggggaact gagaacgagc
     1681 aggatgctcc gccatgccac aggtaacgat ttccgcgctg gcacagcgcg atttacaacg
     1741 ccttcaggac tttctcaaaa ccaaaaatcg gctggcagcc agaaaaggag gtgaggtgat
     1801 cgtccgggct atccagcaac tgaaaacatt gccagacatt ggccgcccgg tgccgtttct
     1861 gccgctggaa tataaggaac tggtgatcgg gtttggcgac agtggctatg tgatgctcta
     1921 ccgccacgac cgggaaatgg accagattgt gatcgtcacc gtcaggcatc agaaagaatc
     1981 cgggtatccg ggggcagaca gcctctaata accgatgacg ggggcgcaat tcattgcgtg
     2041 gccgtcgccg gtttgacaag cgcagcgcgt cagaaaacca catcctgaac ggaaaaacgg
     2101 gtaaaattca gcgatacatt taccgtgaga aattacacac ctttcacacc cgataaaatc
     2161 aggggtatac agtggcgaaa acattgctca acctttcaca ggcagcacaa gccgcgggca
     2221 ttaccagaag aacgctatac aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg
     2281 ggaaaaacaa ccctgtcgtg gatgtgtcag aactgatccg tgtatacgga aatgtgaata
     2341 tccctgagaa acaaattccc ggcatttcac accgtgaaaa tacacaaaaa aatttcccac
     2401 aggaacagct tctggcaatg caaaaggaac tggccgatct ccggcaggct gtcaccctga
     2461 tgctggaaga taaaacatcc cgggaagaag aacgccgcca gcacgatgat gagcgccgga
     2521 aactccaggc cgaagtggac agattgacaa cagaactgac acagaagaaa aaacgcttct
     2581 ggtccggctg gtttagctga gaacttaccg aagggaatgg agaacatgac gaagcaggaa
     2641 aaaactgccc tcaatatggc taaattcatt aaggatcagt cgctgctgtt gctggaaaag
     2701 ctgaacgagc tggacatgga taccccggcc gacatgtgcg aacggcttca cgaggatgcg
     2761 gaaacactct ggcaggtgat gcgggataag ctcggggaag aataacagca ccggccatgc
     2821 cggtgctggc gttatttttt gccgaacggc caccaccggc gggcgggttt gtcctccggc
     2881 agttcaggca ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag ctccgctttc
     2941 agcttctcgt tcaccgcgct ttccccgtgc cgctcgctga ccacctcaat catcgcgtta
     3001 atctgctccg gtgagcactt cccggcatca accacctgca caccgttctc gatggcgaac
     3061 cgctccacct ttcgatagag gtaatcacac agcttcattc ctgtgggttt gtcccgttcc
     3121 cattgcggat tttcaccggt gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg
     3181 tcatacatcc cgatggattt catcatcgcc agatgctggg cccggtcgac aaaacagaaa
     3241 acatacgccc cggtctgcca ggcgagcacc accatgtctt ccagatgctc gctggttggc
     3301 ataatgcagc gctcgccgtc tttgtcttcc acgtcctcaa taaataccat gtacagcgcc
     3361 ataatccctc actccgttgt cataaattta aagcacgcga acagcctctc ccggtccagc
     3421 tccaccgttt tgcgggcctc gccggtctgt ggattcacga tttcatccac cgccagaatc
     3481 gcccgctgta cccggacaag cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg
     3541 ccgttcgccc ggcggttgaa cagatacccg gacaccactt cctgataccg cctgcggctc
     3601 ttgacggtgc gctctacctc aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc
     3661 ggcactttca tccctttcag ggccatacgg tgcaggtacc gccagccggt ggccccccgg
     3721 ccctccatgg agaggcgcag ggactgcacg gccagccggt gcggcagctg cgccgccgac
     3781 acccttgacg gctgaaacgg aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac
     3841 gccaccccgt gcggcgtcag cccccacacg ctgacctgac ggccgtcaac cgggtatttc
     3901 gcccggacca gcagcccttt cgccaccaga ctgtccagga agcggtaaac cggctgtacc
     3961 gccgcaatcc ccagccgtcg cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc
     4021 cagcgcaggg tcagccgtgt tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg
     4081 ttgtactgct ccagtgtcat cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc
     4141 cgccgtcggc cggaggcagg tactccgcca gcgggtccgt tttcgccagc accgggaagg
     4201 ttttcagcga aatttcccgg cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca
     4261 cgcccagccc ggtcatcacc gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa
     4321 acagcggccg cgcctccttc tggatgaagc tgtcgccgct gatgtgctcc acgttccccg
     4381 cctcccggct ggcccggcgg cgctccacgt ccacccggat ctccccggtc tggcccgccg
     4441 cccactgcgc ggactcctgg ctggccgcgc gatagaacca gcggatcggc gtgttatcca
     4501 gtaccgtggt tttcacaaaa tcagcgggca gatcctgccc gcactgaccg aagtcgccca
     4561 gcgactggtg cgcgagcagc aggttgcagt cccggctgcg cagcgtcccc agcgcgttca
     4621 gcacgtactt cgacagcaga tatttaatct catcgagcat gatgctcgcg tgcggccacc
     4681 tccggaactc atcccgcgca atgatgattt gcgcgcagcg ggcaaacagc atcttctgca
     4741 cccggatcac cgcctcatcg tccatcgagc cgatgacgta caggcagccg ccgccgttga
     4801 ttatccccgc cacatcgata ccgctgtccg tctgcaggac gctcaggtcg gccacgttct
     4861 ccagcttggt gatcagcccc ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg
     4921 ccgccagcac ctgctgaatg tttgccccct gcgggaactg ctccgcaatc agcttcgccg
     4981 ctttctgctc gctgatgcgg tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc
     5041 cggcaaccag cagattcttc agggcgtact ggtcgagatc gcggaacaga ttcagctgcg
     5101 ccggtttccc cggccgcaga tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt
     5161 gcggcgccca cgcgtcccct ttggggtcga agtacaccac agcgtccccg aaatcgcgta
     5221 cgcagcgaat gagcgcgttg gtggccatcg cgctcttgcc gctcccgggc aggccgagga
     5281 tctgaatatt ggtcttgcgc caggtatcac gagggaccag caccggctgg ccctgctcat
     5341 ccttacccag aaacaccgcc tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg
     5401 cgttaaccgg taccggctcc ccgaaatgca ggtggcggac gtctgtcatt tctccggtcc
     5461 gctggctgct gcgccggctc tcctgattca gtttcgcgtt caccgcctcg tcagcggccg
     5521 caaaatgacc atcaagcagc aggaacgcca gcagccgcgc cagaaggacg ccggcgacaa
     5581 gccctccggc acaccaggct atcccgaaca ggttagccgg ctgcagcagg taataatctg
     5641 cgttcgcata gaggtccggg cgcaggacgg tttctcccca gctctcgata aacacggcga
     5701 caaccagccc ccccagaagg gccagaccgg cagcgccggt cagccagtac gcgtagttcc
     5761 agacatgtcg ccgtgccctg accatccgcc cgggtgaatc cagcgaggcc agcaccgaaa
     5821 atggcacagt ccggttatta tcgcgcttat agcgatcggc atactcccgc cgcgccttat
     5881 ggctcagccc cacaaaacgc attcggccgg cccagagttc agccagaccg aaaaacaggc
     5941 cgataaccag aaaaaaaccg gccagactgt aatactcccg gtacagccag ctgacccacg
     6001 ggtaaatctg ccgggccagc tccgggatat gccactgcca ggcaagcgaa tccgtattaa
     6061 acatgcaatc tctctccttc agacgacggg tttaccggcg tgatgcgcag cccccgctgc
     6121 acaagccgcg gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt
     6181 cagctttcac cgcggaggcc agcctctttc cgccccatgc ggaaaaggcg cagccccgga
     6241 ggaacaacga gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt
     6301 ccggtgttcc ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca
     6361 ggagcagctt tgctgcttcg tcaggtgcgt ccagcaccgt ggatttttgt gggtggtcag
     6421 gcgtgagcct gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccggacagag
     6481 ccagaaagag gggaaaagac cgtgcgtcgg tggcgtccag ccagcgtaag caaggtcttt
     6541 tcccctgaag tcagccacgc cccagcgggg atgacttgca tttctccgca aggagaaaaa
     6601 agcaacagag ataggttaac gtaaaatgtt cagcgaaaaa ccgacatggt tcacctatcc
     6661 tgataattga tcgtcaggga atagaaagac ttaatcaggg gacaatatcc cacatcagcg
     6721 cgtccggtga ccgtcttgca ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct
     6781 acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt
     6841 ccgtggataa gcaggggact aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt
     6901 tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt tcggtgcatc
     6961 tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt
     7021 aaagatcccc accgtttccg gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc
     7081 cgtttaccgg atacctgtcc gccgttctcc cttgcgggaa gtgtggcgct ttctcatagc
     7141 tcacacactg gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag
     7201 aactccccgt tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc
     7261 cgggaaagca cgcaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga
     7321 tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga
     7381 aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca
     7441 actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa
     7501 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgcg
     7561 atccccgtca gttcagagga ggacgatggt gccaccgttc cgccctgaac atcccggata
     7621 taatttgcct gacatgcggc cagaagaaag ccagcaacaa tcaatataaa aagaaaaata
     7681 gtctttacct ttttcatata ctttccctgt atttaaagac tccggcattc atccctgaac
     7741 accggaagtc agtatttcaa atcatccttg ataatttttt gttaccagtt accgtcccga
     7801 taatcaaaag ctatataaca attatccaca tcaggtttta tttgattttt aaaaaatggc
     7861 tgtagtaaag gaacccattc tttcttcaaa tcaaactcac cattattaat attatccttt
     7921 aaaggcattc ccaagctttc aatgacagaa ccatcgtcac caaagtcttt agaatactct
     7981 tcccctttaa actcttcagt tttcttatca aaccatgtca aacgtaattt aagccccata
     8041 tttacctctc aaagatattt tttaatgttc cgttttggat ctggcccttt aacctgctta
     8101 cccgttttgg gatcaaatgc gcctaggtgt tcgccatcac tggcacgata cccttcaagc
     8161 tcaccgtgct gggaatccca ttcataaatt ttacgttttt tatcgccata ccatcgggcc
     8221 cgcttaccac caccaccttg tttaggtgtt ttaggatcac ctcgctttaa ctctccaagt
     8281 ccctttatgt cttctgtttt aggtactgga tgataatcat ggccgtagtc tttagtcccc
     8341 ttgcgaggct tgttcttttc ctcatttaac ttattttctg catccttcgc ttttttctcc
     8401 ttttgtttcc ggctctccat cgccccggta agtgcagcct tatctctgga taaggattca
     8461 ttcattgcgt ttaaagatgc ctgagccgct tttaatttct cattctgatt ctgaatatcc
     8521 ttatcaagat agcgaacctg atcctgccac ccggagccat attcatgagg cgggttataa
     8581 gtcacaaaat catctttatt tgcctctgcc tcttttacct ttttatcggc ttcctgaaca
     8641 gcagcccttg caccggggag cgccttctct gtcgatgcaa tgcggctgtt taaggtcgca
     8701 atatttttat cttcggcatc cagctcagct ttcgctttat catagtctct tttcagcccc
     8761 tcttccggat gagcggcgtc ccatgcctgc tggcgacgct tttcttcctc ctgacgctgt
     8821 tttacctgtg ccggggtaag aacatctgtc accgacacat aaaccggctt ctgtccggtc
     8881 tctttcggga aacgaataac ggcctcacgg gagttaccac cggctgtaaa accagccgga
     8941 cgtgaatcac ctttttcagc aacaatgcct tttggcgggg ctttcgctgt cggaacacct
     9001 ttcggtacgc tcacctgcag agacgggaga cccggaatcg acacactgaa tacccccgga
     9061 cgttttgtcg gtttcgcatc caccacaggg acactcattg gccgacctgc gacaaccgca
     9121 atatgctgcc gctcatcctt caccacatcc acaacccgtt gtctgacacg aaccgtcgcc
     9181 tggtccagtg gtaaagtact tgccggagtc tccgtcaccg catcggccgg taatgacgtc
     9241 ataatttttg acatcatttt cggatcatct tttgcaatct cagaaggcag cacaccgtag
     9301 atcgcaatcc cccacagacc aaacttaaac ggccccttca gggcagccag cacatcagca
     9361 acggcggccg acaacgcatc gccggaaact gataaagcca gtccttcagc ccccggagta
     9421 gccagggcag gcagaccgaa ggccatcgca gtggccgaag actgtccgcc accagagcta
     9481 ccgctatgat tgccgctgtt accaccatta ccaccgggtt tactcccgcc gttaccaaac
     9541 tcccccggat tatagttatg aatatctcca ttcggcgtat gcgtcgtacc ccagcccggc
     9601 ccgctgtttg gatccgtacc accacctgat gaagggccac ctttaccaga cgtaccgttc
     9661 acattcccac tggcctgacc accattatgt cccagacctg aattacccgg acctcgaccg
     9721 tctccaccac tcataccgtt ttcctctttg acatttaaaa caataaatta aagccaacat
     9781 actgtacata tacacagtat aattattacc ttactcctcc cgttttcagg tgtcaatata
     9841 tgagattagt tctcatcttc aggcacaaaa aagcgcccgg agccacctgt aagcagatcg
     9901 cgtcttttcc cgaaaatttc tcatggcaac aaattttatt atttaagaca ttcatcacac
     9961 ttacatttca gatggtatag acacatctga aagctaactg gcatgaggag aggacctgcg
    10021 gttaaactgt tcgggaaacc tgagacgccc ggaggccact
//
