LOCUS       NZ_CP066761             3306 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain ECP19-198 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP066761
VERSION     NZ_CP066761.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16956340
            Assembly: GCF_016458885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Hoffman,M., Luo,Y., Timme,R. and Baugher,J.
  TITLE     Whole Genome Sequencing of strains utilized in the 2020 Harmonized
            PulseNet GenomeTrakr Proficiency Testing Exercise
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Hoffman,M., Luo,Y., Timme,R. and Baugher,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2020) CFSAN-ORS-DM-MMSB, FDA Center for Food
            Safety and Applied Nutrition, 5100 Paint Branch Parkway, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: HGAP4 v. 0.4
            Assembly Name                     :: CFSAN091030
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 776x
            Sequencing Technology             :: PacBio Sequel; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 17:22:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,584
            CDSs (total)                      :: 5,453
            Genes (coding)                    :: 5,156
            CDSs (with protein)               :: 5,156
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 106 of 297
            Pseudo Genes (incomplete)         :: 177 of 297
            Pseudo Genes (internal stop)      :: 67 of 297
            Pseudo Genes (multiple problems)  :: 46 of 297
            Pseudo Genes (short protein)      :: 4 of 297
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066761.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="ECP19-198"
                     /db_xref="taxon:83334"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA"
                     /collection_date="2019"
                     /collected_by="CDC"
     gene            1..1041
                     /gene="lsoA"
                     /locus_tag="JG533_RS27565"
                     /old_locus_tag="JG533_27565"
     CDS             1..1041
                     /gene="lsoA"
                     /locus_tag="JG533_RS27565"
                     /old_locus_tag="JG533_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="WP_000068433.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            1038..1418
                     /gene="lsoB"
                     /locus_tag="JG533_RS27570"
                     /old_locus_tag="JG533_27570"
     CDS             1038..1418
                     /gene="lsoB"
                     /locus_tag="JG533_RS27570"
                     /old_locus_tag="JG533_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(1653..1976)
                     /gene="mobA"
                     /locus_tag="JG533_RS27575"
                     /old_locus_tag="JG533_27575"
     CDS             complement(1653..1976)
                     /gene="mobA"
                     /locus_tag="JG533_RS27575"
                     /old_locus_tag="JG533_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2248..2442
                     /locus_tag="JG533_RS27580"
                     /old_locus_tag="JG533_27580"
     CDS             2248..2442
                     /locus_tag="JG533_RS27580"
                     /old_locus_tag="JG533_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041624.1"
                     /translation="MNKQQQTALNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2570..2818)
                     /locus_tag="JG533_RS27585"
                     /old_locus_tag="JG533_27585"
     CDS             complement(2570..2818)
                     /locus_tag="JG533_RS27585"
                     /old_locus_tag="JG533_27585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000919062.1"
                     /translation="MAGSEQESARGSFRGETPGIFIVLSGFATSGLSVDFCDARQGGGA
                     YGKTPAVLASSGALLFAHMFFPALSPDSVDNRITAFE"
                     /name="hypothetical protein"
     gene            2929..3042
                     /locus_tag="JG533_RS28055"
     CDS             2929..3042
                     /locus_tag="JG533_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     Typing          complement(1986..2211)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.583"
                     /coverage="99.0"
                     /evalue="1.05e-76"
     Typing          2472..3001
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.413"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(1657..1976)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="4.0199999999999997e-60"
ORIGIN
        1 atggcacaga acccgtttaa agcactaaat atcaatattg acaagattga gtctgctctg
       61 acgcagaatg gcgtcacaaa ctattcctct aatgtaaaaa acgaaagaga aactcacata
      121 tctggcacat ataaaggaat agacttctta ataaaactaa tgccatcagg cggaaatacc
      181 actatcggaa gagcgtctgg gcaaaataac acttactttg atgaaatcgc cttgattata
      241 aaagaaaact gtttgtattc agacacaaag aactttgaat acaccattcc aaaattcagt
      301 gatgatgaca gggcaaatct atttgaattt ctttctgaag aggggataac aataacagaa
      361 gataataata acgatcctaa ttgtaaacac caatatatta tgaccaccag caatggtgac
      421 agggtcaggg caaaaattta caagcgcggc tctattcaat tccaaggaaa ataccttcaa
      481 atcgcgagtt tgattaacga tttcatgtgc tcaatactaa acatgaaaga gattgtcgaa
      541 caaaaaaata aagaatttaa tgttgacata aaaaaagaaa ctattgagtc cgagttgcat
      601 agcaaactac caaaaagcat cgataaaatc catgaagata tcaaaaaaca gctatcatgc
      661 tcgctaataa tgaaaaaaat agatgtcgaa atggaagatt actcaacata ctgcttctct
      721 gcattaagag ccatagaagg ctttatatat caaatactta atgatgtttg caatccatca
      781 tcatcaaaga accttggcga atacttcact gaaaacaaac ccaaatatat aatcagagaa
      841 atacaccaag aaactataaa tggtgaaata gcggaagttt tgtgtgaatg ctacacttac
      901 tggcatgaga acaggcatgg tttgtttcat atgaaaccag gaatagctga cacgaagaca
      961 attaacaaat tagaatcaat cgcaatcatc gataccgttt gccaattaat agatggtggc
     1021 gtagctaggt tgaaattatg aaaaaagata aaaaatatca aatagaagca ataaaaaata
     1081 aagataaaac tttatttatt gtctatgcta ctgatattta tagcccgagc gaatttttct
     1141 caaaaatcga atccgacttg aagaaaaaga aaagcaaggg tgatgttttt tttgatttaa
     1201 taattcctaa cggtggaaaa aaagatcgtt acgtctatac gtcatttaat ggcgagaagt
     1261 tttcaagtta cacattaaac aaagttacga aaactgatga atataatgat ttatctgagc
     1321 tctcggcttc gttctttaaa aaaaactttg ataagatcaa cgtaaacctt ctatccaaag
     1381 ccacatcatt tgctttgaaa aaaggcattc caatataata tcaagccaga agacatttac
     1441 cataacgtct cattacgcgc accgccaaaa cgcgctgaac gcgattctga cgcagacacc
     1501 gcaaaaagag atgttttccc cggggtaaac cggaaaaatg cgtcaggatc gctttcagct
     1561 cactgcatag ctatgcatga aagtgaatgg cgatcggttt gggggcttac ggcgttcata
     1621 ccgtctgttt tcgacagttt ctctccggga agctaatctg ccataagcct ggataacagg
     1681 gcacggtgat actccgtaat agcgatcagc acctccgcat actctctgtc cccgacacgc
     1741 ttgccgtcga taaagagttt tttctgcaac gcccccagtc gcataacttc tttcagcacc
     1801 cggtcatcag tcagtgagtt aactttctta ccgagagctg ccgcccgtaa aaaaccagaa
     1861 acggtcttgc cgcattcagc agcccttttt ctgatttcct ggtcttcttc tgccgtcaga
     1921 cgaacagggc gactgatagc ccgcctgcgc gtattacttc cacttcgttt tgtcataaaa
     1981 catggctccg tatctgacat ggggtgtcgg ggcgaagccc tgaccagggt aattgtaata
     2041 gcgtgcatgt atgcgcggta taacaattgc acatcctgtc ctgtctgcaa gttcgaatta
     2101 ccaacaaagc actgctgttt tttggcagtg acagtgttgt ctgactgaaa tgtccgtcgg
     2161 cagaataccg gcggtaagac ggtcgaaaag tggtgcgcga tataatcaca cgacgcagat
     2221 gacagacaga acggagaaaa cagaaagatg aacaagcagc agcaaactgc actcaacatg
     2281 gcgggattca taaaaagcca gagcctgacg ctgctcgaaa aactggacgc actcgatgct
     2341 gacgagcagg ccaccatgtg tgagaagctg cacgaactcg cagaagaact ccagaacagc
     2401 atacagacac gctttgaagt agaaaatagc acagagatat aacgctcagg gcaggatgcc
     2461 gaacggcaca acagagcgta aggagaaaat gtcacataag gcgctcttcc gcttcctcgc
     2521 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggtgtcagct cactcaaagg
     2581 cggtaatacg gttatccaca gaatcagggg ataaagccgg aaagaacatg tgagcaaaaa
     2641 gcaaagcacc ggaagaagcc agcaccgcag gcgtttttcc ataggctccg cccccctgac
     2701 gagcatcaca aaaatcgacg ctcaagccag aggtggcgaa acccgacagg actataaaga
     2761 taccaggcgt ttccccccgg aagctccctc gtgcgctctc ctgttccgac cctgccactt
     2821 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2881 tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2941 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3001 agacacgact tatcgccact ggcagcagcc attggtaact gatttagagg agtttgtctt
     3061 gaagttatgc acctgttaag gctaaactga aagaacagat tttggtgact gcgctcctcc
     3121 aagccagtta ccttggttca aagagttggt agctcagcga accttgagaa aaccaccgtt
     3181 ggtagcggtg gtttttcttt atttatgaga tgatgaatca atcggtctat caagtcaacg
     3241 aacagctatt ccgttgacaa aaaaacaaga tctgtacata ttcaaaaaaa acaacggatt
     3301 gaccca
//
