LOCUS       NZ_CP066752             6675 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain ECP19-2498 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP066752
VERSION     NZ_CP066752.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16946945
            Assembly: GCF_016458945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Hoffman,M., Luo,Y., Timme,R. and Baugher,J.
  TITLE     Whole Genome Sequencing of strains utilized in the 2020 Harmonized
            PulseNet GenomeTrakr Proficiency Testing Exercise
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Hoffman,M., Luo,Y., Timme,R. and Baugher,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2020) CFSAN-ORS-DM-MMSB, FDA Center for Food
            Safety and Applied Nutrition, 5100 Paint Branch Parkway, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: HGAP4 v. 0.4; Unicycler v. 0.4.7
            Assembly Name                     :: CFSAN091029
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 501x
            Sequencing Technology             :: PacBio Sequel; Illumin MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 17:18:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,793
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 308
            CDSs (without protein)            :: 308
            Pseudo Genes (ambiguous residues) :: 0 of 308
            Pseudo Genes (frameshifted)       :: 116 of 308
            Pseudo Genes (incomplete)         :: 169 of 308
            Pseudo Genes (internal stop)      :: 71 of 308
            Pseudo Genes (multiple problems)  :: 43 of 308
            Pseudo Genes (short protein)      :: 4 of 308
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066752.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="ECP19-2498"
                     /db_xref="taxon:83334"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2019"
                     /collected_by="CDC"
     gene            complement(join(6651..6675,1..167))
                     /locus_tag="JG532_RS28595"
                     /old_locus_tag="JG532_28595"
     CDS             complement(join(6651..6675,1..167))
                     /locus_tag="JG532_RS28595"
                     /old_locus_tag="JG532_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            235..558
                     /locus_tag="JG532_RS28600"
                     /old_locus_tag="JG532_28600"
     CDS             235..558
                     /locus_tag="JG532_RS28600"
                     /old_locus_tag="JG532_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            767..>1204
                     /locus_tag="JG532_RS29115"
                     /pseudo=""
     CDS             767..>1204
                     /locus_tag="JG532_RS29115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1254..1796
                     /locus_tag="JG532_RS28610"
                     /old_locus_tag="JG532_28610"
     CDS             1254..1796
                     /locus_tag="JG532_RS28610"
                     /old_locus_tag="JG532_28610"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000536290.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1803..2036
                     /locus_tag="JG532_RS28615"
                     /old_locus_tag="JG532_28615"
     CDS             1803..2036
                     /locus_tag="JG532_RS28615"
                     /old_locus_tag="JG532_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2526..2771)
                     /locus_tag="JG532_RS28620"
                     /old_locus_tag="JG532_28620"
     CDS             complement(2526..2771)
                     /locus_tag="JG532_RS28620"
                     /old_locus_tag="JG532_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3035..5128
                     /gene="cda"
                     /locus_tag="JG532_RS28625"
                     /old_locus_tag="JG532_28625"
     CDS             3035..5128
                     /gene="cda"
                     /locus_tag="JG532_RS28625"
                     /old_locus_tag="JG532_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286659.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            5125..5388
                     /locus_tag="JG532_RS28630"
                     /old_locus_tag="JG532_28630"
     CDS             5125..5388
                     /locus_tag="JG532_RS28630"
                     /old_locus_tag="JG532_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            5458..5604
                     /locus_tag="JG532_RS28635"
                     /old_locus_tag="JG532_28635"
     CDS             5458..5604
                     /locus_tag="JG532_RS28635"
                     /old_locus_tag="JG532_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            6311..6499
                     /locus_tag="JG532_RS28640"
                     /old_locus_tag="JG532_28640"
     CDS             6311..6499
                     /locus_tag="JG532_RS28640"
                     /old_locus_tag="JG532_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024175185.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTYLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     BGC             1..6675
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6480..6566
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          5834..6018
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          549..2143
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcgtgcga ggcagctgcg gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg
       61 tcagcctggt cgtcggcgtc cagttcattc agtttttcca gcagggtcag cgtctggctt
      121 ctgatgaagc gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc
      181 catgtgtggg gagtctgtcg gaggttttta atgataccgg tgaaacgcga aaggatgctc
      241 acgatacggg tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa
      301 cttgcgtcat ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt
      361 cctacactgg caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag
      421 atagcccgga agataaacag tggacagtgg tcggggcatg accgggttca tgtggtggct
      481 gcactgatgg ccatcggacg tgaactgagt gagctgaggg atgaggtccg taaacaggga
      541 gaacgcgatg atagttaagt ttcatgccag gggaaaaggc ggtggcagtg gtccggttga
      601 ttacctgctg ggccgggagc gtaaccggga aggggcgaga gttctgcggg gtgctcccga
      661 agaggtgcgg gaactcatcg atgccacgcc gtttgcgaaa aaatacacct cgggggttct
      721 gtcttttgca gagcagacac tgccaccggg agagcgtgaa agggtgatgg agagctttga
      781 gcgggtactg atgcccggtc ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca
      841 ggacaagggg cgtcttgagc tgaattttgt catcccgaat atggagctgg cgagcggaaa
      901 acggctccag ccctattacg accgggcgga ccgtccgcga atcaatgcct ggcagacact
      961 ggtgaatcac cattacgggc tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt
     1021 caccccgaat aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact
     1081 ggaggctctt taccatgccg gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac
     1141 agcggcaggt tttgaggtgg tgaggacaac caggagcagt atcagtatcg ccgacccgga
     1201 gggcgggcgt aatctcaggc tgaaaggggc gctgtatgag caatctttta caaatgggga
     1261 cagacttcga gaagaaactg aaagagcgtg ccgtctctac agagaacatg ctgaacagcg
     1321 agttcaggaa gctcgaagcg tctgtagaca gggaactgaa cttaaacggg cagaaaatca
     1381 gagacgctat cagcgcacac acagcagcgg tgaaagaaca actggagacg ctgaacagtg
     1441 ccgtgaacgc gcagttcagc gccacagaga acgaactggc ccggcggcag gaagagctgc
     1501 tctggaagct ggtcaaaggg cggatactgt acccgtcgct gacggcgctt tgcgtcacgg
     1561 gcgggatttt tctggcgtca tgggggctga ttcagtggca ggaaagccgt attgcggcga
     1621 acatcctggc tatcagggac caggaagaga cgctggcgaa actccgggaa aaaacatggg
     1681 gtgtgacgta tcacgagggc aggaacggga aatttctggt gttgccgtcc ggggtgaagg
     1741 gggaaaacaa ctggacggtg gagaagaaga acgcagtaag actggtcagg gagtaagtga
     1801 gtatgacaga gctggaaatg cacttactga acgccttaga gcaattgcag caggactata
     1861 cgcagcggct gaaagactgg gagagcgcct tcacggattt gcagcatatg ttcgagctga
     1921 cacgacagga gaacgggcaa ctgagggaac agtgcgacga cttgagtcag caggtgcaga
     1981 gattagccgg gcaggtggac cgcttgagtc ggttatttat cacgaacagc agataaggga
     2041 tgaacaggaa aggcaggcac gcctggaacg ggagcgtgcg cggtcctggc atgaagagca
     2101 ggaacgggaa gcacgggtat ggcgtggccc gtcaatgggg atgtgacggt gagttgttcc
     2161 gggaggttgc cagatggtac cggaacgcgt taaaaacgca tccgggcgcg attctggcgt
     2221 gtcgtttttg ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct cagcctcatc
     2281 ccgcgatgtc tgcgtatgtc atcaactgaa aagatgggaa aaaaacagtg tcatgaggtt
     2341 tacgcaggca aaaccagcgt tattcacatg gctgaatagc tatgcatgcg gttaaaattt
     2401 atcaggtgcg atcgcggcag cttttcgggt ggtttgttgc catttttacc tgtcagcagc
     2461 cgtgatcgcg ctgaacgcat ttcagcggtg cgtacaataa ggggttatgt taaataaccc
     2521 ttagcttagc ggcggcgcag tggttgccag gtgaaggtgt catccgggtc ctgtccggca
     2581 gtcagtgtgg cttctcgatt tttgctggcc gttcgttcat caagaatggc tttaagtatc
     2641 cgtgcccgga ggcgtcgctt tgtttcctgc ggcattttgt cctttttcgt cagccccatg
     2701 gcacgagctt cagcggcaat ggcttctgcc agtcggcgct cttcggcttc ggtcagtcta
     2761 tggcgtttca tggtggtctc cgggtgtttc tctggtgtca taacagttta actgttggct
     2821 ctctccttat gctaatcagc cttctttcat gcctgttgcc attgagttta agaatgattg
     2881 gttgtttttc ttgagcgttt tcacaaaaac gtaaatccag tgggttgaca ggatttacga
     2941 ggtgacgtaa ctttttactg tacataaaac cagtggtttt atgtacagtt tgttgttttt
     3001 aacgcattgt tttaaaagtc aaagaggtgt ttttatgagt gattacgaag gtagtggtcc
     3061 gacagaaggt attgattatg ggcactcgat ggtcgtgtgg ccgtcaacag gactgatttc
     3121 gggtggtgat gtgaaaccag gaggctcatc aggtatcgct ccatccatgc ctccgggatg
     3181 gggggattac agcccacagg gtatcgcact tgtacaaagt gttctttttc cgggaattat
     3241 tcgccggatt attctggata aggaacttga agagggagac tggtcgggat ggtctgtcag
     3301 tgtgcatagc ccttggggaa acgagaaagt ttccgctgca cgaacagttc ttgagaatgg
     3361 tttacgtggt ggtttgccag aaccgtctcg cccggctgct gtttcttttg cccgtctgga
     3421 gcctgcttcc ggaaatgagc aaaaaattat tcgtcttatg gttacacagc aactggaaca
     3481 ggtaacggat attccggcca gccagttacc agcagcgggt aataatgtac cggtgaaata
     3541 tcgtctgatg gaccttatgc agaacggtac gcaatatatg gctattatcg gaggtattcc
     3601 gatgacagtg ccggtagttg atgccgttcc agttccggac cggagtcgtc cgggaacaaa
     3661 tattaaagat gtttacagtg cccctgtatc accaaatcta ccggacctgg tattaagtgt
     3721 gggtcagatg aatactccgg ttctgtctaa tcccgaaatt caggaagaag gagttattgc
     3781 tgagacaggt aattatgttg aggctggtta tacgatgtcc agtaataatc atgatgtcat
     3841 tgttcgtttt cctgaaggca gtggtgtttc tccgttatat atttcaactg tagagattct
     3901 ggacagtaat ggtctgagtc agcgtcagga agccgaaaat aaagcaaagg atgatttcag
     3961 agtcaagaaa gaagaagcgg tagcccgggc tgaagctgaa aaagcaaaag ccgagttatt
     4021 tagtaaagca ggtgtgaacc agcctcctgt atatacacag gaaatgatgg aaagggctaa
     4081 ttcagtaatg aatgagcagg gagctcttgt tctgaataac actgccagtt ctgtacaact
     4141 ggcgatgacc ggaactggtg tctggactgc tgctggtgat attgcaggca acataagcaa
     4201 gtttttcagt aatgctctgg agaaagtaac catacccgaa gtgagtcccc tgcttatgcg
     4261 gatttctctt ggtgctctgt ggtttcattc agaagaagct ggagcaggaa gtgatatcgt
     4321 gccggggcga aatctggagg cgatgttttc gctgagtgct cagatgttgg ctggacaggg
     4381 cgtggtcatt gaacctggtg cgacgagtgt aaatctgcct gttcgtggac aattgataaa
     4441 cagtaacggg caattagctc tggatttact gaaaacaggg aatgaaagta tcccagctgc
     4501 ggttcctgtt cttaatgctg tccgtgatac agcaacagga ctggataaaa tcacgttacc
     4561 agcagtagta ggcgcacctt cccggacgat tctggttaat ccggtaccac aaccttcggt
     4621 gccaacagat acaggtaatc atcaacccgt tccggttaca ccagtgcata caggaacgga
     4681 agtaaaaccg gtcgaaatgc cagtaacgac gattactcct gtttctgatg ttggtggact
     4741 acgagatttt atttactggc gtcctgatgc tgctgggact ggtgttgaag ctgtttatgt
     4801 gatgcttaat gatcctctgg attcagggcg attttcgcgt aaacaacttg ataaaaaata
     4861 taaacatgct ggtgattttg gtattagtga tacaaaaaag aatcgtgaaa ctcttactaa
     4921 attcagggat gctattgagg agcatttatc ggataaggat acagtagaga aaggaacata
     4981 ccgaagagaa aaaggttcaa aagtttattt taatcctaat acgatgaatg tggttataat
     5041 taagtcaaat ggtgagttct tatctgggtg gaaaataaat ccagatgcgg ataatggtcg
     5101 aatttattta gagacaggtg aactatgaac aagatggcaa tgatcgattt ggcgaaatta
     5161 tttttagctt cgaaaattac agcaattgag ttttcagagc gaatttgtgt tgaacggaga
     5221 agattgtatg gtgttaagga tttgtctccg aatatattaa attgtgggga agagttgttt
     5281 atggctgctg agcgatttga gcctgatgca gatagggctg attatgaaat tgatgataat
     5341 ggacttaagg ttgaggtccg atctatcttg gaaaaattta aattataatc atgagtgatt
     5401 ttaatatctg gcatttaagt ataaatgtca gatattcact catgacaagg agatgtcatg
     5461 aaaaaaaaga tagggggatc tatgattctc gttcttgctg ttttgtgtct gactgcttgc
     5521 caagctaact atgtaaggga tgttcagggt ggaacaattg ctccatcatc atcttcgaaa
     5581 ctaatcgggg tggcggttca gtgaaaaata ttaaaggatc ttcttgagat cctttttttc
     5641 tgcgcgtaat ctgctgcctg taaacgaaaa aacccgcctt gtggcgggtc cttccgtccg
     5701 atcagaagtc ttcgacctct tctaatcggc ggtggtaact ggcttggagg agcacagtca
     5761 ccaaaactgt cttttcagtt tagccttaac tggttcataa cttcaagagc aactcctcta
     5821 aatcagttac caatggctgc tgccagtggc gttaagtcgt gtcttttccg ggttggcccc
     5881 aagagaacag ttaccggaga aggtgcagca gtcgggctga acggggggtt cctgcataca
     5941 gcccagcccg gagcgaactg cctaagtcgc cccgagatac cagaagacgt gagctatgag
     6001 aaagcgccac gtgtcccgca agggagaaag gcggaaaagg tatccggtaa tcgatatggc
     6061 gggaacgtgg gggcgcagga gggagcgatt caccggaaac ggtgatgatc tttaagccac
     6121 gtcgggtttc gccaccgctg gcggatttca tggttgagcc tcacggctcc cacagatgca
     6181 ccgaaaaagt gtctgttttg tgaagtccgt caggggggcg gagcctatgg aaaaagcgcc
     6241 tccggcgcgg cctttttcgg ggttttcgtt tcttgctcac atgttctttc cggcgttatc
     6301 ccctgattct gtggataaca ctatcaccgc ttttgagtgg gcagataccg ctcgccgcag
     6361 ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc tggacgagca
     6421 ttttctcctt acgtatctgt gcggcatttc acacccggca tggtgcactt ttcataccat
     6481 cctcactgat gccacatgat taagccagta tacactccgc tatcgctacg tgactgggtc
     6541 atggctgcgc cccgacaccc gctaaaaccc gctgacgcgc cctgacgggc ttgtctgctc
     6601 ccggcatccg ctcacaggcc agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     6661 tcaccgtcat ccccg
//
