LOCUS       NZ_CP066678             6311 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 163_1 plasmid p5, complete
            sequence.
ACCESSION   NZ_CP066678
VERSION     NZ_CP066678.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038111
            Assembly: GCF_016415485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 137.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:34:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 2,989
            Genes (coding)                    :: 2,842
            CDSs (with protein)               :: 2,842
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 53 of 147
            Pseudo Genes (incomplete)         :: 94 of 147
            Pseudo Genes (internal stop)      :: 38 of 147
            Pseudo Genes (multiple problems)  :: 31 of 147
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066678.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 163_1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p5"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            447..533
                     /locus_tag="JCP82_RS15200"
                     /old_locus_tag="JCP82_15200"
     CDS             447..533
                     /locus_tag="JCP82_RS15200"
                     /old_locus_tag="JCP82_15200"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(654..1343)
                     /locus_tag="JCP82_RS15205"
                     /old_locus_tag="JCP82_15205"
     CDS             complement(654..1343)
                     /locus_tag="JCP82_RS15205"
                     /old_locus_tag="JCP82_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1350..1628)
                     /locus_tag="JCP82_RS15210"
                     /old_locus_tag="JCP82_15210"
     CDS             complement(1350..1628)
                     /locus_tag="JCP82_RS15210"
                     /old_locus_tag="JCP82_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(1588..2502)
                     /locus_tag="JCP82_RS15215"
                     /old_locus_tag="JCP82_15215"
     CDS             complement(1588..2502)
                     /locus_tag="JCP82_RS15215"
                     /old_locus_tag="JCP82_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2484..2864)
                     /locus_tag="JCP82_RS15220"
                     /old_locus_tag="JCP82_15220"
     CDS             complement(2484..2864)
                     /locus_tag="JCP82_RS15220"
                     /old_locus_tag="JCP82_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3195..3398)
                     /locus_tag="JCP82_RS15225"
                     /old_locus_tag="JCP82_15225"
     CDS             complement(3195..3398)
                     /locus_tag="JCP82_RS15225"
                     /old_locus_tag="JCP82_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(3410..3943)
                     /locus_tag="JCP82_RS15230"
                     /old_locus_tag="JCP82_15230"
     CDS             complement(3410..3943)
                     /locus_tag="JCP82_RS15230"
                     /old_locus_tag="JCP82_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(4393..4563)
                     /locus_tag="JCP82_RS15235"
                     /old_locus_tag="JCP82_15235"
     CDS             complement(4393..4563)
                     /locus_tag="JCP82_RS15235"
                     /old_locus_tag="JCP82_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(4579..5112)
                     /locus_tag="JCP82_RS15240"
                     /old_locus_tag="JCP82_15240"
     CDS             complement(4579..5112)
                     /locus_tag="JCP82_RS15240"
                     /old_locus_tag="JCP82_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(5133..6083)
                     /locus_tag="JCP82_RS15245"
                     /old_locus_tag="JCP82_15245"
     CDS             complement(5133..6083)
                     /locus_tag="JCP82_RS15245"
                     /old_locus_tag="JCP82_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     Typing          complement(5134..6082)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1592..2502)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacggtaaag aggtttaaaa agaggtttaa aaagagatat aaaaagaggg gggctaattt
       61 tcccgcctaa aaaccacttc ttttttcttt aaacaggttc gcgcattttt caaaactgcc
      121 aatccaaaac cttgcttcct acggtcgcca aaaaggtgtg atcgctacgc tctcacattg
      181 tactgcgcag tcgcctaaag gctcctttgc taaaaacatg ttggtaaaga ggcctttgag
      241 cggttctgaa tgcttatcta agccgtttta agacagtctt ggataaaaat ccttatctag
      301 ctttaaagcg ccttacaggc ggtcagggca agccctaaca accctaaaat aagcgatact
      361 taaaaacacc acacaagttt aattttttaa gatataataa cttgtcgcct atccgacagt
      421 cttgcgactg gaaggaggtg tatctaatgt ttgaattgtt catttgcccc cttcttatag
      481 aagtttgtgt ctctttattc agttattggt tgaataaccg aaacaagaag taaaatcaat
      541 aaaggcgaca tcaacctgaa aaaaagcccc agagaatccg acctctctgg ggctttcgtg
      601 tatctaatgt ttgatcattt gcatgtttag tttaacatga ttgccgatca aattcaattt
      661 tgctgttgct gatctaacca ttccttgaca ctttttagat aatttaatgc cgtttctttt
      721 ttcgttggat tttgtatgtt cgttgcttgc tgaagcagtg ctttataacg ttcgtaggta
      781 actgattgtt gccgttcttt tcgttcctgt tcactagttg aaaaagggat tgtatcactg
      841 aataaattat aacaagcttc ttctgtctgc gtatctttaa aatattgcac gtcttttttg
      901 gttcgctcat agttgtcttg aacctctttt tgctgttttt ctggcaagtt ttgataaaga
      961 cgagtgagtt tttccatgtt tttatccgtc aaacgttttt cttgattcac aaaaatatcg
     1021 ttcatttcct cataacaagt ggtaaactct tccatttttt gtgttaacac ttgctccttt
     1081 ttctttttat cacatgctgc ttcatacttt tgagtgatat cccctaataa aatacttctt
     1141 gaaatacgtt tatttccctc taaaacgccg aataaaccac ttaaaacgat tgataatgta
     1201 aatataaccc ctggcaataa aaacctctta aatcgaattt tagagccttt taatgaatat
     1261 cttgtttgat aagccttata gctaagccaa ataattccta aaatactggc aaaaatgagt
     1321 agatagggta aatagaatgc catttctatt tattctcccc ataccagcca aaaggctaac
     1381 agtgtcatga ttaagcaaca taagggggca agaatatcca ttgatcgctg cataattact
     1441 tttttctgtt ctttatttcg ctggtttaat agcgcatggt ggaatttttt gacttgtgaa
     1501 tcaattgtag tttgtattct ttctttggta taaggtgcaa gaaaagcacg ttgtttcttt
     1561 atttcatttt ctatgtcttg taacgtttta gaacgataat tcttgttggt agtctgctcc
     1621 tctttcaacg tgttggtcat ggtttcttcg agtcggtcta atttccggtt gttcagtcgt
     1681 aagagttcgt tgttctcttc gtacttcttt tgcagtttcg cgaatgctag ctcgagctct
     1741 tcgtagctcg ttggttcgtt gttgattgta ggctcgttct cgtctgtcca gctcatgtaa
     1801 aatcgtctcc ttttcgtaat cctttcctag tttgttggat cgtacttttt tctttttgtg
     1861 gatcaattcg taagtaaaat ttttcccacg attcaagcaa tgaatgccga atgttttaag
     1921 agaatcacaa aattggttat acgttcgagt ttttttgttt ttcattgttt gatcaatcgc
     1981 aaaccgaatt tcgtctttcc aaacaggttc atttttttct tttaatttga tttcagacat
     2041 ggttcgtttt tcgggtttct tccctcgttc agtgattgtt aagtcatgtt caagacaaat
     2101 ctcgtcattt aggtcataac atttgtttaa aaatgcttga ccatgggctt gaaatttgtt
     2161 cccagtttcc agattgacgg agttaatgac aatgtggtta tgaatatggt ctttgtctgt
     2221 atgtgtgtaa atcgcgattt ggtggtcagg ggctattttt ttcgcatact ctgcccctag
     2281 ttgattggct ttttcagcgg taatttctcc tggtcgaaaa gactggatca ataagtgtgc
     2341 ttgtacacga tcgtcttttc catacagcat gcgaatttgt ttcatttcgc ttttggcata
     2401 atcaatgtca cagtttatcc ctgttttgac ggttgctcgt ggttcggcgt aattgatcgc
     2461 tcggctacaa gaagtggttc ggcttacttt aactgttgcc atatcttttg tagctccttt
     2521 tgcatgattt ttaggttttc ggataagcgt tgggcgtcgc caggatcaac gtcgtgttgg
     2581 tgtgtgttgc accacttggc aatttgattg atattcccgc cagctttggc taaatcgcgc
     2641 gcgattgcct gagcaatgtc tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc
     2701 ttggctttca cgaaagccgg cacactcatt tgcaaagctt cagctgactg cttcagcttt
     2761 gcaaattcgg attcgctcac tcgaaaactg atttgttttg gctcggatcg ataggtatat
     2821 tttttagaat gattgctagc caaatattga ttttgttcgc tcataagtcc caccctttca
     2881 gtacgtccta cggacgtatc gttcgcaata ccattgtacc taaaaaggta caataagtaa
     2941 agctcactaa agctcactaa agggcttggc aagctctata tttgctagcc aaatacctcc
     3001 gtatttggtg caaataaccg cttgtcaaag gagcgcaagc tcccccttga atccccctca
     3061 aaatccacgc tccacaatac aaaaaaaggc tagcatttgc tagccacttt ttgtaaagtt
     3121 tcgcttattt caccgaaggt gaaattgttg ctttgtaata ttcaaacaac cctattcttc
     3181 taaaatgtat cgatttattt ttttttagac tttattatgt ctaagacaac taagattaac
     3241 actaaaaata aaccagattt gtataattgc cctccgctta gcatattaac aattatagca
     3301 acagctatag caaatataaa aatacgaata gtagctattt gcggattctt tttagtaaat
     3361 gtttttaatg attttaatga tttagatatc aaaaacatca ctccttaact tataacgttg
     3421 aatatctagt agctacaact cttccagctg catcatatcc cctttggtaa taaaatccaa
     3481 tccagccatt accatttttg tcccattctt ttgcaactgc attccctgga gaagcacctt
     3541 ttttagtcca acttgcacct gttaacaaag caccaattgc ccaacctggg ataccaaaca
     3601 tagaagtgct agctttccct cctgcccaaa aggcagcatt aatagcatta gcagctactc
     3661 cagtggttac agcaatattt gtatattcct ttttgcccca tcttgcaact gcagctcttg
     3721 tcatgatatc tgaagaattt gcaaaatctt ccgcactata accatcctca tatttagtta
     3781 cgaccatacc atcttcagtg tactcaataa ctacattttc agtagcattg ttttcgttta
     3841 atgataccac ttgggaattt tctgaagcat aaactatagt agttgacggt aaaacaactg
     3901 ccccaatagt taacaaagct acccctaaag taaatttttt catagaattt cctcctgata
     3961 cagaaaaata tatagaaaac gttttctttg tgaaaatcat aaaacaaaag gtataggctt
     4021 gtcaatattt tatttagtaa agataattta tatttttgat aatgtggtat agttagaatg
     4081 cattaggaaa atatgttata ttaaattctt gttgacataa tgtacactat gccaagtagc
     4141 ccttgtatca accgatacag aggctcactt tgtgattttc gattggtcta ggtcaaaagc
     4201 tctttttaag cttgattttc tgcggttgct tctttccgtt tctttgtgaa tttttaccag
     4261 tcatttttta ctgggttgtt atttttaata aaatatgata aatttttggt agcgcttcat
     4321 aagcgcgttg ctttttcaag aataaacact cattattttt taactggctg ctcgaaagag
     4381 cagtcttttt tattatttat ctgtttcttt tacaaacaat tcttcgttag gactgttgta
     4441 ataatttgga ttcaatttca attcaccatg tttattccct ttattgatca ctgacaataa
     4501 catcggactg aatcctagaa ttcctagtaa aatagaaccg aatagaagat tttttcttgt
     4561 catgaatgtt ccctccagtt atttccaaag ttgccaccat tttttttcag tatcaatttg
     4621 tttgtctatt gtttctgttg cttcagaaag agtcgttgag tcattctctg gagcttcttc
     4681 tgtataagtc aactgtagtt tttcttgcag tttttcgatt tgtttgttgg cttgtagggt
     4741 gagttgttgt tgctggtcga gtagtttttg tatatgagaa ttattttgct ctctgagggc
     4801 aagttgttct cgtaaaaatt cattttcttc acgcaactga tctagcaact tgtttgtaac
     4861 cacgtttttg cttggtatat taacattttt atcgttgtct tgttcgtact cgtgcaacct
     4921 atctagcaac atttggttgc ctaagttgcg agcaagttgc aatactccag aacgcaatac
     4981 aaccttataa ccacgctcat ttttagctat catggaagaa tctaattttt ggatatgttt
     5041 cgatattgct tgcctactca tcttaaaaag ttctgacaat tcctttattg taaaggtttc
     5101 ttgtggttgc atactggttg cctcctaggt tgctactgga ataaattatc taaatcagat
     5161 aataaacttt ttttctcttt tttcgaaagt gcggactctg tttgtttttc aagagcttct
     5221 ttctcttttt ctagttgttt gatttgttgg aggagttcaa gttctcgttt cttctgtttg
     5281 attttttcgt taattgttgt agcaggttct tcgtattctt tttgtttatc agctaaagcc
     5341 aatgctttat tttgttcttc tttggttggt gcatgtttat taaaatcatc tgcttttttg
     5401 atttctggtt taaacgtaaa ttgatagcca acgacaggtt ttcctcgccc ttttccgtat
     5461 ttctttttga ttgataatcc tttgaagatt gcggttaatt cctgtcgtat aggctttata
     5521 acacgattat caacgtttgc ttggttgttc cagtaacttt ttggaatatc taatagttca
     5581 tgaaaatcct ctttagaaaa ataagcaaaa cctttagttc gatattgttt tagtaatcga
     5641 aacatagtct ttgcatagct actttgtaat tcgttaaatt gttggagtga atagcgaacc
     5701 cactcatcta aattgtttag taaaggtaaa gctttttcgt aaacttgtat gtctacatat
     5761 ggatctttag cagttccacg tattttaaac tcactaaaca taacgaatct atctatatcg
     5821 agcccattag cacttctttt accaaaacgt aaatccatta atttatcata agtcatttct
     5881 aaatcattaa tgaaacgatt attagcagtt gctttataat tacttaaatc ttttagttgg
     5941 tcaaaagtaa gacggactgt tttatcgcct ttttcgcgca ttctagacac aatagagaaa
     6001 aatagattca tttcaactgg tgtaaatttc cgcaacggaa tggtatttaa ttcatgatgg
     6061 tatttcacga tttcatttgc caaagatatt cctccttact ccttcaatgg atacagacgt
     6121 tataaacaac gttttgtctg tgataatgca tattataaca tacgtgagtg caaaaaaaga
     6181 gtttaaaagc agacaaaatg tagtttaaaa gcagacaaaa tgtagtttaa aagcagacaa
     6241 aatgtagttt aaaagcagac aaaatgtagt ttataaaacg ctgtatccat tgaaggagta
     6301 aggacacagc t
//
