LOCUS       NZ_CP066677             7165 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 163_1 plasmid p4, complete
            sequence.
ACCESSION   NZ_CP066677
VERSION     NZ_CP066677.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038111
            Assembly: GCF_016415485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 137.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:34:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 2,989
            Genes (coding)                    :: 2,842
            CDSs (with protein)               :: 2,842
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 53 of 147
            Pseudo Genes (incomplete)         :: 94 of 147
            Pseudo Genes (internal stop)      :: 38 of 147
            Pseudo Genes (multiple problems)  :: 31 of 147
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066677.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 163_1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            join(6356..7165,1..291)
                     /locus_tag="JCP82_RS15155"
                     /old_locus_tag="JCP82_15155"
     CDS             join(6356..7165,1..291)
                     /locus_tag="JCP82_RS15155"
                     /old_locus_tag="JCP82_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="WP_224198038.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="cysteine protease staphopain"
     gene            264..707
                     /locus_tag="JCP82_RS15160"
                     /old_locus_tag="JCP82_15160"
     CDS             264..707
                     /locus_tag="JCP82_RS15160"
                     /old_locus_tag="JCP82_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            1011..1391
                     /locus_tag="JCP82_RS15165"
                     /old_locus_tag="JCP82_15165"
     CDS             1011..1391
                     /locus_tag="JCP82_RS15165"
                     /old_locus_tag="JCP82_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1373..2287
                     /locus_tag="JCP82_RS15170"
                     /old_locus_tag="JCP82_15170"
     CDS             1373..2287
                     /locus_tag="JCP82_RS15170"
                     /old_locus_tag="JCP82_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2247..2525
                     /locus_tag="JCP82_RS15645"
     CDS             2247..2525
                     /locus_tag="JCP82_RS15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            2527..3216
                     /locus_tag="JCP82_RS15175"
                     /old_locus_tag="JCP82_15175"
     CDS             2527..3216
                     /locus_tag="JCP82_RS15175"
                     /old_locus_tag="JCP82_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031592678.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     KEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3337..3423)
                     /locus_tag="JCP82_RS15180"
                     /old_locus_tag="JCP82_15180"
     CDS             complement(3337..3423)
                     /locus_tag="JCP82_RS15180"
                     /old_locus_tag="JCP82_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_109746925.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            4068..4805
                     /locus_tag="JCP82_RS15185"
                     /old_locus_tag="JCP82_15185"
     CDS             4068..4805
                     /locus_tag="JCP82_RS15185"
                     /old_locus_tag="JCP82_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            4834..5355
                     /locus_tag="JCP82_RS15190"
                     /old_locus_tag="JCP82_15190"
     CDS             4834..5355
                     /locus_tag="JCP82_RS15190"
                     /old_locus_tag="JCP82_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_031592680.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENNNAI
                     TTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            5439..5891
                     /locus_tag="JCP82_RS15195"
                     /old_locus_tag="JCP82_15195"
     CDS             5439..5891
                     /locus_tag="JCP82_RS15195"
                     /old_locus_tag="JCP82_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025089292.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKVMLKNHTKTLILLFHFVIFLSLVFTLIQSKFFYFSHS
                     IATWLLIFFLSLLLVINTLIIIFNKHKNLFLHSVFPIILLSFLYFV"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          4069..4805
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1374..2284
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtggtgttt atcctaaaaa agtagcacga atactaaacc tttcagaggt ccaaacacaa
       61 ctaagaaaag gaaatgccat ttatatggga actgctggtt atggaaatta tagtaacagc
      121 cgtcatgcac ttgcattatt tgggtgggtc aatcaaggtg gttatcaaac ttactatgtg
      181 tggaatcctt ggtataacta cccaactgta ataagtgcaa atgcaggacc agttacaatt
      241 cctgtacctg gaggtggttt tacttgggac aataccatta caaattggta aataaaccca
      301 ttgctttagt tatgggtcta gtgttagttc ttttcttaac tagttgttca aaaaaacaag
      361 aaatacaaaa gactgatctg attccttttc taggaacttg gcaaactaaa gatcattcag
      421 aacgtctagt aatcactaat ttaaatactt caaaaaatat agaacaatat accatcttag
      481 taaaagagaa caatcataaa attcaaatca ttgataaaaa tttaaatata aatgaacaag
      541 ttttcattta caaagcaaca aataatttac taacactgcg ctctaataaa aaacaattag
      601 attacacttt tagtttggat tctccaaaaa agctcgtatt tattttgaca acgaatgatg
      661 ggacagaagg gacagctaaa ccaattgaat tttttaaagc tgattgatga atagttgttt
      721 gatattacaa aacaataatt tcaccgaagg tgaaacaagc gaaactttac aaaaagtggc
      781 tagcatttgc tagccttttt tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg
      841 agcttgcgcc cctttgaaaa gcggttattt gctagccaaa tacgtaggta tttggctagc
      901 aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt tttaggtaca
      961 atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac
     1021 aaaatcaaaa tttggctagc gatcattcta aaaaatatac ctatcgatcc gagccaaaac
     1081 aaatcagttt tcgagtgagc gaatctgaat ttgcaaagct gaagcagtca gctgaagctt
     1141 tgcaaatgag tgtgccggct ttcgtaaaag ccaaggcaca aaacgcgcgt ctggtgacac
     1201 cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga
     1261 atatcaatca aattgctaag tggtgtaata cacaccaaca cgacgttgct cctggagacg
     1321 cccaacgctt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac
     1381 agttaaagta agccgaacca cttcttgtag tcgtgcaatt aattacgctg aaccacgagc
     1441 aaccattaaa acaggaataa actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat
     1501 tcgcatgctg tatggaaaag acgaccgtgt acaggcacac ttattgattc aatcttttcg
     1561 accaggagaa attaccgctg aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat
     1621 agcccccgat caccaaatag ctatttacac acatacagac aaagaccata ttcataatca
     1681 cattgtcatt aactccgtta atctggagac tggaaaaaaa tttcaagctc atggtcaagc
     1741 atttctaaac aaatgttatg acctaaatga cgagatttgt cttgcacata acttaccaat
     1801 cactgagcga ggaaagaaac cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa
     1861 aaatgaacct gtttggaaag acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa
     1921 aaaaaccaga acctatgacc aattttgtaa ttccctcaaa acattgggga ttcattgctt
     1981 caatcgcggg aaaaatttca cttacgaatt gatccacaaa aagaaaaaag tacgatccaa
     2041 cagactagga aaggactacg aaaaggagac gattttacat gagctggaca gacgagaacg
     2101 aacctataac caacagcgaa ccaacgagct acgaagagct cgagctagca ttcgcgaaac
     2161 tgcagaagaa gtacgaagag aacaacgaac tcttacgcct gaacaaccgg aaattagacc
     2221 ggctcgaaga aaccatgaac aacacgttga aagaggaaca gaccaccaac aagaattatc
     2281 gctctaaaac gttaaaagac atagaaaacg aaataaagaa acaacgtgct tttctcgttc
     2341 cttatacgca agaaaggata caaactacga ttgatttaca agttaaaaaa ctccatcaag
     2401 agctattaga ccagcgaaat aaggaacaga aaaaggtgct tattcaacga tcattggatt
     2461 ttcttgcccc cttatgttgc ttagtcctga cactgttcgc attttggtta gtatggggag
     2521 gctaaaatgg cattttattt accctatcta ctcatttttg ttagtatttc aggaagtatt
     2581 tggctcatct ataaaatttt tcaaacaaga tattcattaa aaggctctaa aattcgattt
     2641 aagcgatttt tctttcttgg ctgtatattt tccttgataa tcgtttcaag cggcttattc
     2701 ggcgttttag agggaaataa gcgcgtttct agaagtattt tattaggaaa tgtcactcaa
     2761 aagtatgaat cagcgcgtga taaaaagaaa aaagagcaag cattagcaca aaaaatggaa
     2821 gagtttacca cttgttataa ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg
     2881 acggataaaa acatggaaaa actcactcgt ctttatcaaa acttgccaga aaaacagcaa
     2941 aaagaggttc aagacaacta tgagcgaacc aaaaaagacg tgcaatatgt aaaagataca
     3001 aagatagaag aagcctgtag tgatctattt ggagacacaa acccttggtt tgccagtgaa
     3061 gaagaaaaaa aagaaaaaca acaatctgtc acatatgaac gctacgaaaa cctgtttcaa
     3121 caagcaacta acatgcaaaa tccgacaaaa aaagaaaccg ccttaaacta tctaaaaagt
     3181 gtcaaggaat ggttagatca gcaacagcaa aattgaattt gattgaaact catgttaaac
     3241 tgaatgtgca aatgatcaaa cattagatac acgaaagccc cagagaggtc ggattctctg
     3301 gggctttttt tcaggttgat gtcgccttta ctgattttac ttcttgtttc ggttattcaa
     3361 ccaataactg aataaagaga cacaaactcc tacaagaagg gggcaaatga actgttcaaa
     3421 cattagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt tattataccc
     3481 tacaaaatta aatctgtgtg gcgattttaa gtatacctta ttttcgggtt gttagggctt
     3541 gccctgaccg cctgtaaggc gcttctcgac tagattaagg gagttaatca agaattgtct
     3601 tagaacggct tagacaagct ctcagattct cccacctcct ctttgaacaa cggttttagc
     3661 aaaggagccg gaggcgactg cgcaggtaca atgtgagagc gtagcgatca caccttttta
     3721 gcgcccgtag ggagcaagtt ttctagttta gaaaaattcg cgaacctttt tggaggtaaa
     3781 agtaggagtt ttttaggcgg gaaattaaat gtgctcttaa gatatactgt tagatatact
     3841 gttagatata ctgttaataa taactgttag ggattagcaa ccttatggga gagtaagtat
     3901 tgacataggt agtgggggat aaattgtcac actatggggg ataaattgtc acactatggg
     3961 ggataaattg tcacgctatg ggggataaat cgtcacacta tgggggataa attgtatttt
     4021 acaccctctt atgtctatga taacataatt tattgaggtg atttttattg agtaacgata
     4081 tagttcgata taataatgga ttaaattctg tccctttaag aaaatttacc cctatagata
     4141 tggatttatt ttggggaata tgttctaaaa tgaaaagaaa aggtacaaaa gaagaaattt
     4201 ttacatttaa acaaataaaa gaattaattg attacaatat aaatgcacgt ggcgaccgtt
     4261 ttgctaatga tttaataaaa atgtcaaaaa aaatcattac tttgtctttt atttatgaag
     4321 acgaaaaaac atatgaacaa ttaaatctgt ttcaaaaatt taaaatcgat aaagtgaatg
     4381 aaacattatt aataaaggtt tctgaagaat ttgaatatat acttaattca atcggaacta
     4441 attttactcg tttcgaatta gaaaatatga caaacttaaa gagtagctac gttaaagagt
     4501 tatatcgcca acttatggct cataaagaca gaagatctaa aaaaggtcac tggtttgtaa
     4561 aaatagaaga ttttagagct ctattagcaa taccagaaag ttatcgcatg agagatattg
     4621 ataaacaaat ttttgcccaa gcgaaaaaag aatttttaaa cgttaattca actcaccctg
     4681 ctatttttaa aacttttgaa atcaaaaaaa ttaaagccta caaaggaaat aagataggat
     4741 ctctgcaaat ttattttgaa gaaaatatac cttcaattac cttagataat tggttagaga
     4801 aataataacc taacaattta ggaggcaatt atcatgcaac ccaaagaaac ctttacaata
     4861 aaggaattat ctgaattatt tgaaataagt aggcaagcaa tttctaaaca tgttgataaa
     4921 ttagacccgt ccttattagc taaaaatgag cgtggttata aggttgtatt acgttctgga
     4981 gtattgcaac ttgctagcaa cttaggtaac caaaggttac tagataggtt gcatgagtat
     5041 gaacaagaaa acgataaaaa cgtttatata tcaagaaaaa acgtggttgt aaacgagttg
     5101 ctagataagt tgcgtgaaga aaatgaattt ttacgagaac aactttctct caaagagcaa
     5161 aataattcta atatgcaaaa attactcgac cagcaacaac aactcacctt gcaggcaaat
     5221 caacagatcg aaaaactgca agaacagtta caattagctt atacggaaga acctgaagaa
     5281 gaggtattaa cttctgtcaa taaagaaaac aacaatgcaa taacaacaga aaaaaagtgg
     5341 tggaaatttt ggtaacaatt accaactttc cataaagaag aaatcaattt ctatagactt
     5401 tatctttcta aaaataaaaa atatgtttag gtgatcaaat gactataagt aaagtaatca
     5461 aacaaaaaag aatagaaaaa caactaactc aagaagattt agctgaaatg cttttagtat
     5521 ctaaaaaaac catttctaat tgggaaaatg gtagaacaat accagataca gaaaatttaa
     5581 ttcaatttgc taagttattt gatttatcac tagacaatat tttgttagga gaagaaaaag
     5641 ttatgttaaa aaatcataca aaaacactaa tactattatt tcattttgta atttttttaa
     5701 gtttagtttt taccttaatt cagtccaaat tcttttattt tagccattca attgccactt
     5761 ggttattaat attttttcta agtttgctat tagtaatcaa tactctgatt attattttta
     5821 ataaacataa aaatttattt cttcatagtg tttttccaat tattttgctg agttttttat
     5881 actttgtata aagtataaaa aaagactgct ctttcgagca gccagttaaa aaaataatga
     5941 gagtttattc ttgaaaaagc aacgcgctta taaagcgcta ccaaaatttt atcatatttt
     6001 ataaaaaata acaatccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     6061 gcaactgcag aaaatcaagc cttaaaaaaa gcttttgacc tagaccaatg aaaaatcaca
     6121 aagtgagcag ctatagccct tgatagaggg gctacttggc atagtcgaca ttatgttaac
     6181 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     6241 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     6301 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     6361 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     6421 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     6481 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     6541 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     6601 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     6661 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     6721 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     6781 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     6841 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     6901 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     6961 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     7021 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     7081 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     7141 tcaaataata aaatatgcaa attac
//
