LOCUS       NZ_CP066671             6303 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 158 plasmid p4, complete
            sequence.
ACCESSION   NZ_CP066671
VERSION     NZ_CP066671.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038107
            Assembly: GCF_016415565.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 163.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 03:00:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,879
            Genes (coding)                    :: 2,773
            CDSs (with protein)               :: 2,773
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 37 of 106
            Pseudo Genes (incomplete)         :: 65 of 106
            Pseudo Genes (internal stop)      :: 24 of 106
            Pseudo Genes (multiple problems)  :: 17 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066671.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 158"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            445..531
                     /locus_tag="JCP74_RS14690"
                     /old_locus_tag="JCP74_14690"
     CDS             445..531
                     /locus_tag="JCP74_RS14690"
                     /old_locus_tag="JCP74_14690"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(652..1341)
                     /locus_tag="JCP74_RS14695"
                     /old_locus_tag="JCP74_14695"
     CDS             complement(652..1341)
                     /locus_tag="JCP74_RS14695"
                     /old_locus_tag="JCP74_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1586..2500)
                     /locus_tag="JCP74_RS14700"
                     /old_locus_tag="JCP74_14700"
     CDS             complement(1586..2500)
                     /locus_tag="JCP74_RS14700"
                     /old_locus_tag="JCP74_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2482..2862)
                     /locus_tag="JCP74_RS14705"
                     /old_locus_tag="JCP74_14705"
     CDS             complement(2482..2862)
                     /locus_tag="JCP74_RS14705"
                     /old_locus_tag="JCP74_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3184..3387)
                     /locus_tag="JCP74_RS14710"
                     /old_locus_tag="JCP74_14710"
     CDS             complement(3184..3387)
                     /locus_tag="JCP74_RS14710"
                     /old_locus_tag="JCP74_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(3399..3932)
                     /locus_tag="JCP74_RS14715"
                     /old_locus_tag="JCP74_14715"
     CDS             complement(3399..3932)
                     /locus_tag="JCP74_RS14715"
                     /old_locus_tag="JCP74_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(4382..4552)
                     /locus_tag="JCP74_RS14720"
                     /old_locus_tag="JCP74_14720"
     CDS             complement(4382..4552)
                     /locus_tag="JCP74_RS14720"
                     /old_locus_tag="JCP74_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(4568..5101)
                     /locus_tag="JCP74_RS14725"
                     /old_locus_tag="JCP74_14725"
     CDS             complement(4568..5101)
                     /locus_tag="JCP74_RS14725"
                     /old_locus_tag="JCP74_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(5122..6072)
                     /locus_tag="JCP74_RS14730"
                     /old_locus_tag="JCP74_14730"
     CDS             complement(5122..6072)
                     /locus_tag="JCP74_RS14730"
                     /old_locus_tag="JCP74_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     Typing          complement(5123..6071)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1590..2500)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtaaagagg tttaaaaaaa ggtttaaaaa gagattataa aagagggtga caaaatttcc
       61 cgcctaaaaa ccacttcttt tttctaccaa caggttcgcg catttttcaa aactgccaat
      121 tcaaaacctt gctccctacg gtcgctaaaa aagtgtgatc gctatgctct cacattgtac
      181 tgcgcagtcg cctaaaggct cctttgctaa aaacaagtca gtaacggagg tgggggaagc
      241 ctctgatagc tcgtctaagt cgttttaaga cagtcttggg taaaaaacct tatcttgttc
      301 ttaaagcgcc ttacaggcgg tcagggcaag ccctaacaac ccgaaaataa gggatactta
      361 aaaacaccac acagatttta ttttgtaagg tataataact tgtcgcctat ccgacagtct
      421 tgcgactgga aggaggtgta tctaatgttt gaacggttca tttgccccct tcttgtagaa
      481 gtttgtgtct ctttattcag ttattggttg aataaccgaa acaagaagta aaatcaataa
      541 aggcgacatc aacctgaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgta
      601 tctaatgttt gatcatttgc aggttcagtt taacatgatt gctaatcaaa ttcaattttg
      661 ttgttgctga tccaaccatt ccttgacact ttctagataa tttaatgccg tttctttttt
      721 cgttggattt tgtatgtttg ttgcttgctg aagcagtgtt tcataacgtt cgtatgtgac
      781 cgattgttgc cgttcttttc gttcttgttc actggttgaa aaagggattg tatcgctaaa
      841 taagttacaa caagcttctt ctgtctgcgt atctttaaaa tattgcacgt ctttttttac
      901 ttgctcatat ttttcttgta cctctttttg ctgtttttct ggtaagtttt gataaaaacg
      961 agtgagtttt ttcatgtttt tatctgttaa acgtttctct tgatccacaa aaatattgtt
     1021 catgtcctca taacaagcgg taaattcctc cattttttgt gctaatgctt gctctttttt
     1081 ctttttatca catgctgctt catacttttg agtgatatcc cctaataaaa tgcttcttga
     1141 aacacgttta ttaccctcta aaacgccgaa taaaccactt aaaacgattg ataaggcaaa
     1201 tataaccccg accaataaaa atcgcttaaa tcgaatttta gagcctttta atgaatatct
     1261 tgtttgataa gccttatagc taagccaaat aattcctaaa atactggcag aaatgagtag
     1321 atagggtaaa tagaatgcca tgtctgttta ttctccccat accaaccaaa aggctaataa
     1381 tgttagtact aagcaacata agggggcaag aatatccatc gatcgttgaa ttaaagcctt
     1441 tttttgttct tggtttcgct ggtctaatag cgcttggtgg agttttttga cttgtgaatc
     1501 catcgtagtt tgtatccttt cttgggtata aggcgcaaga aaggcacgtt gtctctttat
     1561 ttcgttttcg atgtctttta acgttttaga gcgataattc ttgttggtgg tctgttcctc
     1621 gttcaacgtg ttgttcatgg tttcttcgag tcggtctaat ttccgattgt tcaggcgtaa
     1681 aagttcgttg ttctcttcgt acttcttctg cagtttcgcg aatgctagct cgagctcttc
     1741 gtagctcgtt ggttcgttgt tgattgtagg ttcgttctcg tctgtccagc tcatgtaaaa
     1801 tcgtctcctt ttcgtaatcc tttcctagtt tgttggatcg tacttttttc tttttgtgga
     1861 ccaattcgta agtaaaattt tttccgcgat tcaagcaatg aatgccgaat gttttaagtg
     1921 aatcacaaaa ttgggtatac gttcgagttt ttttgttttt cattgtttga tcaatcgcaa
     1981 accgaatttc gtctttccaa accggttcat ttttttcttt taatttaatt tcagacatgg
     2041 ttcgtttttc ggttttcttt cctcgttccg tgattgttaa gtcatgttca agacaaatct
     2101 cgtcatttag gtcataacat ttgtttaaaa atgcttgccc atgggcttga aattttttcc
     2161 cagtttcaag attaacggag ttaatgacaa tgtgattatg aatatggtct ttgtctgtat
     2221 gtgtgtaaat cgcgatttgg tggttagggg ctattttttt cgcatactct gcccctagtt
     2281 gattggcttt ttcagcggta atttctcctg gtcgaaagga ctgaatcaat aagtgtgctt
     2341 gtacacgatc gtcttttcca taaagcgtgc gtatttgttt catttcactt ttggcataat
     2401 caatgtcaca gtttattcct gttttgacgg ttgctcgtgg ttctgcgtaa ttgattgctc
     2461 ggctacatga agtggttcga cttactttaa ctgttgccat atcttttgta gctccttttg
     2521 catgattttt aggttttcgg ataaacgttg agcatcatca ggagcaacgt cgtgttggtg
     2581 tgtattgcac cacttggcaa tttgattgat attccctcca gctttggcta aatcgcgcgc
     2641 gattgcctgg gcaatgtctg gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt
     2701 ggctttcacg aaagccggca cactcatttg caaagcttca gctgactgct tcagctttgc
     2761 aaattcggat tcgctcactc gaaaactgat ttgttttggc tcggatcgat aggtatattt
     2821 tttagaatga tcgctagcca aattttgatg ttgttcgctc ataagtccca ccctttcagt
     2881 acgtcctacg gacgtatcgt tcgcaatacc attgtaccta aaaaggtaca atgagtaaag
     2941 ctcactaaaa gggtttggca agctctatat ttgctagcca aatacctccg tatttggtgc
     3001 aaataaccgc ttgtcaaagg agcgcaagct cccccttgaa tccccctcaa aatccacgct
     3061 ccacaataca aaaaaaggct agcatttgct agccactttt tgtaaagttt cgcttatttc
     3121 accgaaggtg aaattgttgc tttgtaatat tcaaacaacc ctattcttct aaaatgtatc
     3181 gatttatttt tttttagact ttattatgtc taagacaact aagattaaca ctaaaaataa
     3241 accagatttg tataattgcc ctccgcttag catattaaca attatagcaa cagctatagc
     3301 aaatataaaa atacgaatag tagctatttg cggattcttt ttagtaaatg tttttaatga
     3361 ttttaatgat ttagatatca aaaacatcac tccttaactt ataacgttga atatctagta
     3421 gctacaactc ttccagctgc atcatatccc ctttggtaat aaaatccaat ccagccatta
     3481 ccatttttgt cccattcttt tgcaactgca ttccctggag aagcaccttt tttagtccaa
     3541 cttgcacctg ttaacaaagc accaattgcc caacctggga taccaaacat agaagtgcta
     3601 gctttccctc ctgcccaaaa ggcagcatta atagcattag cagctactcc agtggttaca
     3661 gcaatatttg tatattcctt tttgccccat cttgcaactg cagctcttgt catgatatct
     3721 gaagaatttg caaaatcttc cgcactataa ccatcctcat atttagttac gaccatacca
     3781 tcttcagtgt actcaataac tacattttca gtagcattgt tttcgtttaa tgataccact
     3841 tgggaatttt ctgaagcata aactatagta gttgacggta aaacaactgc cccaatagtt
     3901 aacaaagcta cccctaaagt aaattttttc atagaatttc ctcctgatac agaaaaatat
     3961 atagaaaacg ttttctttgt gaaaatcata aaacaaaagg tataggcttg tcaatatttt
     4021 atttagtaaa gataatttat atttttgata atgtggtata gttagaatgc attaggaaaa
     4081 tatgttatat taaattcttg ttgacataat gtgtactatg ccaagtagcc cttgtatcaa
     4141 ccgatacaga ggctcacttt gtgattttcg attggtctag gtcaaaagct ctttttaagc
     4201 ttgattttct gcggttgctt ctttccgttt ctttgtgaat ttttaccagt cattttttac
     4261 tgggttgtta tttttaataa aatatgataa atttttggta gcgcttcata agcgcgttgc
     4321 tttttcaaga ataaacactc attatttttt aactggctgc tcgaaagagc agtctttttt
     4381 attatttatc tgtttctttt acaaacaatt cttcgttagg actgttgtaa taatttggat
     4441 tcaatttcaa ttcaccatgt ttattccctt tattgatcac tgacaataac atcggactga
     4501 atcctagaat tcctagtaaa atagaaccga atagaagatt ttttcttgtc atgaatgttc
     4561 cctccagtta tttccaaagt tgccaccatt ttttttcagt atcaatttgt ttgtctattg
     4621 tttctgttgc ttcagaaaga gtcgttgagt cattctctgg agcttcttct gtataagtca
     4681 actgtagttt ttcttgcagt ttttcgattt gtttgttggc ttgtagggtg agttgttgtt
     4741 gctggtcgag tagtttttgt atatgagaat tattttgctc tctgagggca agttgttctc
     4801 gtaaaaattc attttcttca cgcaactgat ctagcaactt gtttgtaacc acgtttttgc
     4861 ttggtatatt aacattttta tcgttgtctt gttcgtactc gtgcaaccta tctagcaaca
     4921 tttggttgcc taagttgcga gcaagttgca atactccaga acgcaataca accttataac
     4981 cacgctcatt tttagctatc atggaagaat ctaatttttg gatatgtttc gatattgctt
     5041 gcctactcat cttaaaaagt tctgacaatt cctttattgt aaaggtttct tgtggttgca
     5101 tactggttgc ctcctaggtt gctactggaa taaattatct aaatcagata ataaactttt
     5161 tttctctttt ttcgaaagtg cggactctgt ttgtttttca agagcttctt tctctttttc
     5221 tagttgtttg atttgttgga ggagttcaag ttctcgtttc ttctgtttga ttttttcgtt
     5281 aattgttgta gcaggttctt cgtattcttt ttgtttatca gctaaagcca atgctttatt
     5341 ttgttcttct ttggttggtg catgtttatt aaaatcatct gcttttttga tttctggttt
     5401 aaacgtaaat tgatagccaa cgacaggttt tcctcgccct tttccgtatt tctttttgat
     5461 tgataatcct ttgaagattg cggttaattc ctgtcgtata ggctttataa cacgattatc
     5521 aacgtttgct tggttgttcc agtaactttt tggaatatct aatagttcat gaaaatcctc
     5581 tttagaaaaa taagcaaaac ctttagttcg atattgtttt agtaatcgaa acatagtctt
     5641 tgcatagcta ctttgtaatt cgttaaattg ttggagtgaa tagcgaaccc actcatctaa
     5701 attgtttagt aaaggtaaag ctttttcgta aacttgtatg tctacatatg gatctttagc
     5761 agttccacgt attttaaact cactaaacat aacgaatcta tctatatcga gcccattagc
     5821 acttctttta ccaaaacgta aatccattaa tttatcataa gtcatttcta aatcattaat
     5881 gaaacgatta ttagcagttg ctttataatt acttaaatct tttagttggt caaaagtaag
     5941 acggactgtt ttatcgcctt tttcgcgcat tctagacaca atagagaaaa atagattcat
     6001 ttcaactggt gtaaatttcc gcaacggaat ggtatttaat tcatgatggt atttcacgat
     6061 ttcatttgcc aaagatattc ctccttactc cttcaatgga tacagacgtt ataaacaacg
     6121 ttttgtctgt gataatgcat attataacat acgtgagtgc aaaaaaagag tttaaaagca
     6181 gacaaaatgt agtttaaaag cagacaaaat gtagtttaaa agcagacaaa atgtagttta
     6241 aaagcagaca aaatgtagtt tataaaacgc tgtatccatt gaaggagtaa ggacacagcc
     6301 gac
//
