LOCUS       NZ_CP066663             7940 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 155 plasmid p4, complete
            sequence.
ACCESSION   NZ_CP066663
VERSION     NZ_CP066663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038106
            Assembly: GCF_016415505.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:49:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 77 of 138
            Pseudo Genes (internal stop)      :: 32 of 138
            Pseudo Genes (multiple problems)  :: 16 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066663.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 155"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            1..945
                     /locus_tag="JCP86_RS15680"
                     /old_locus_tag="JCP86_15680"
     CDS             1..945
                     /locus_tag="JCP86_RS15680"
                     /old_locus_tag="JCP86_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_199019029.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..1499
                     /locus_tag="JCP86_RS15685"
                     /old_locus_tag="JCP86_15685"
     CDS             966..1499
                     /locus_tag="JCP86_RS15685"
                     /old_locus_tag="JCP86_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_196921171.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1638..2888)
                     /locus_tag="JCP86_RS15690"
                     /old_locus_tag="JCP86_15690"
     CDS             complement(1638..2888)
                     /locus_tag="JCP86_RS15690"
                     /old_locus_tag="JCP86_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_002305888.1"
                     /translation="MKLFIGLDVSSQKLDTCFLTDSKEILFEGSLSNDLIGASEIKQRI
                     LEYQETFGFYQIVIGMESTSVYSFHPAMFFYLDEDLKLLPVEVTVENPYRIKQYSRMFD
                     QDKTDKIDAQIIADYLRVDLHTLSPIKEEVYVGLQRLTRSRYQLVTQMVEAKQHFLENL
                     TYKCNTLSKELANEDNKTSIFGASIMNLLTEGITLDEFSEMPLEAAVELLQKLGKGRFK
                     NPEQLVKSIQKAVRSSYRLGKVTKNSIDLVLGIIANEIKFLKKQIKEIEKAITALLETI
                     PEAQCLLSVPGLGAVYTAGLIAEIGQIERFDDQSKLAKYAGLTWTKNQSGNSQSENTPL
                     NRSGNRYFRYYLIEATNRVRSTLPEFKEYYQKKRNEVPKHKHKRALVLTGRKFVRLIDA
                     LLRNGQFYQPAKVVIEK"
                     /name="IS110 family transposase"
     gene            3281..3451
                     /locus_tag="JCP86_RS15695"
                     /old_locus_tag="JCP86_15695"
     CDS             3281..3451
                     /locus_tag="JCP86_RS15695"
                     /old_locus_tag="JCP86_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            3906..4130
                     /locus_tag="JCP86_RS15700"
                     /old_locus_tag="JCP86_15700"
     CDS             3906..4130
                     /locus_tag="JCP86_RS15700"
                     /old_locus_tag="JCP86_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            4150..4437
                     /locus_tag="JCP86_RS15705"
                     /old_locus_tag="JCP86_15705"
     CDS             4150..4437
                     /locus_tag="JCP86_RS15705"
                     /old_locus_tag="JCP86_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            4887..5267
                     /locus_tag="JCP86_RS15710"
                     /old_locus_tag="JCP86_15710"
     CDS             4887..5267
                     /locus_tag="JCP86_RS15710"
                     /old_locus_tag="JCP86_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_197669149.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5249..6163
                     /locus_tag="JCP86_RS15715"
                     /old_locus_tag="JCP86_15715"
     CDS             5249..6163
                     /locus_tag="JCP86_RS15715"
                     /old_locus_tag="JCP86_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6123..6401
                     /locus_tag="JCP86_RS15990"
     CDS             6123..6401
                     /locus_tag="JCP86_RS15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            6408..7097
                     /locus_tag="JCP86_RS15720"
                     /old_locus_tag="JCP86_15720"
     CDS             6408..7097
                     /locus_tag="JCP86_RS15720"
                     /old_locus_tag="JCP86_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1..7940
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.788"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5250..6160
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaga agctcttgaa aaacaaacag agtccgcact ttcgaaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagaactt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctcgc aacttagaca
     1141 atcaaaggtt gctagataag ttgcataaac atgaacaaaa caacgataaa aatgcttata
     1201 taccaaacaa aaacgtggtt gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaacttgcc ctcaaagagc aaaataattc tcatatacaa aaactactcg
     1321 accagcaaca acaactcacc ctgcaagcca accaacaaat cgaaaaactt caggagcaac
     1381 tgcagttgac ttatacggaa gagtcttcag agaatagttc aacgactctt tctgaagcaa
     1441 cagaaacaat ggacgaacaa acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa
     1501 gcctaatggt aataagtcaa gatataaatt ttagaatatg agttagattt tgaaaaatca
     1561 gaccgcgctg aactagaccc gaccaaaatc aaattttttt cgggttgatc acgaagattt
     1621 tcgcaaataa ctttacgtta tttttctatc accaccttcg ctggctgata gaattgaccg
     1681 ttgcgtagta gcgcatcgat caagcgtaca aattttcttc cggttaagac gagcgctcgt
     1741 ttatgtttgt gtttaggtac ttcatttctc tttttttggt agtactcttt aaattctgga
     1801 agtgtgcttc ttactcgatt ggtggcttca attaagtagt aacgaaaata gcgatttcca
     1861 gaacgattta gtggcgtgtt ttcagattga gagtttcccg actgattttt agtccaagtt
     1921 agaccagcat acttcgctaa tttggattga tcatcaaatc gctcgatttg accaatttca
     1981 gcgattaagc ccgcagtata aacagctcct aatcctggga cacttaataa gcattgggct
     2041 tctggaattg tttcaagtaa agcagtaatg gctttttcaa tttctttaat ctgttttttt
     2101 agaaacttga tttcattggc gattatacct aatacaaggt caatggaatt cttggttact
     2161 ttacctaaac gataagaact acgtacagct ttttgaatac ttttgacaag ctgctctgga
     2221 tttttgaatc gaccttttcc tagcttttga agtaattcaa cggccgcttc cagaggcatt
     2281 tctgaaaatt catctaaggt aatgccttct gtaagtagat tcataatcga agctccgaat
     2341 atggaagttt tattatcttc attcgcaagt tcttttgata aagtattaca tttatacgtc
     2401 aaattttcta aaaaatgttg ttttgcttct accatttgtg tcactaattg ataacgagat
     2461 cttgttagtc gttgaagtcc aacataaact tcttctttaa taggagacaa tgtatggaga
     2521 tctactcgca gataatcagc tatgatctgt gcatcaattt tatctgtttt atcctgatcg
     2581 aacattcttg aatactgttt gattctataa ggattttcta cagtaacttc gacaggtaat
     2641 agcttcaaat cttcatctaa gtagaaaaac attgctgggt ggaaactata aacagaagtt
     2701 gattccatac ctatgacaat ttggtagaaa ccaaaggttt cttggtattc gagaattctt
     2761 tgtttgattt cgctggctcc gattaaatcg ttgctaagtg aaccttcaaa caagatttct
     2821 ttggagtctg ttaaaaaaca agtatctaat ttttgagaac taacgtctaa accaataaat
     2881 aatttcatgg aaggtcctcc tttcataaat tttttggaat acttagctat tgaagtttat
     2941 ccctgaatct tattgaataa ccgcagtctc gcttaatagt actcaattgt ttacttctta
     3001 aaagccaccc tatttcctac tagaaatagt ttaagaagtg aacggtacag ctaatgagtt
     3061 tgaggcatga taatcaataa gtcgggatgc aggcttcctg ctgtagtcat agctacaaag
     3121 gaaattagca gtgttccgat agaccgtttc cataagtcta ttatccagga acaaaattca
     3181 atagccaagg tttaaaagtt atttaataag gaatagccct aaggggtgac tgaaacgatg
     3241 gaagtcgttt caaaatatat attacgagaa ggaaatactt atgacaaaaa aacacttgct
     3301 attcggttct atcctactag gaatattaag ctttagccca atgttattat cagtggccaa
     3361 caaaggaaat aaaaaagggg aactaaaact caatcctaat tactacaaca gtcaaaatga
     3421 ggaagtattt gtagaaaaac ttgataaata agaaaagact gctcgtacga gcagtctttc
     3481 tgaaagagat tatcctatta ttaaccttag aaaagaataa taaatgaata tgagatatgt
     3541 aaaaagcata acaaacacaa acaaaaataa caagtcttta aaaaatgacc gatcaatatt
     3601 cacaaagaat tagaaagaaa caaccgcaga aaaccaagcc ttaaaaagag cttttaacct
     3661 agaccaataa aaattcacaa aatcagcgcc tggatccctt ggtacaggcg ttacttggta
     3721 gtatacacat tatgtctact agctgtttaa ataactgatc ttataatttt atttaaacga
     3781 ttatgaattc aaaactactt tttatgacgt taatcattgt tatataataa aatgttatat
     3841 aataaaatta ctaagattat ttaatataac aagcaatttt aaaaaaacga aaaggaaaaa
     3901 caatcatgaa aaagaaagta ttaaaacatt gtgttattct aggaatatta ggaacttgtc
     3961 tagctggcat cggtacagga ataaaagttg atgcagctac ttactatgga aatggtcttt
     4021 attgtaacaa agaaaaatgt tgggtagatt ggaatcaagc taaaggagaa attggaaaaa
     4081 ttattgttaa tggttgggtt aatcatggtc catgggcacc tagaaggtag tggtagggag
     4141 gttattataa tggattttac taaagaagaa aaacttttaa atgcaattag taaagtatac
     4201 aatgaagcaa ctatagatga ctatcctgac ttaaaagaaa agctctttct ttattctaaa
     4261 gaaatcagtg agggaaaaag tgttggtgaa gttagtatga aattaagtag ttttcttgga
     4321 agatatattt taaaacataa atttggatta cctaaatctt taatagaatt acaagaaatt
     4381 gttagtaagg aatctcaagt atatagagga tgggcttcta ttggtatttg gagttaattc
     4441 tttggaataa aatgaaaatg ggactgaaga atcaattctg attcatcagt cccataaatt
     4501 atagagccga aaaattatca tatttataga tttattaata gtaaatggca taagaatctt
     4561 tgaacaacga aaaaaatgta gctctaccta attattgatt tgcaacaatt tcaccgaagg
     4621 tgaaacaagc gaaactttac aaaaagtggc tagcatttgc tagtcttttt tgtattgtgg
     4681 agagtggggg ggagggggat tcaaggggga gcttgcgccc ctttgacaag cggttatttg
     4741 caccaaatac gtaggtattt ggctagcaaa tatagagctt gccaagccct ttagtgagct
     4801 ttagtgagct ttacttattg taccttttta ggtacaatgg tattgcgaac gatacgtccg
     4861 taggacgtac tgaaagggtg ggacttatga gcgaacaaca tcaaaatttg gctagcgatc
     4921 attctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga gtgagcgaat
     4981 ccgaatttgc aaagctgaag cagtcagctg aagctttgca aatgagtgtg ccggctttcg
     5041 tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca gacattgctc
     5101 aggcaatcgc gcgcgattta gccaaagctg gcgggaatat caatcaaatt gccaagtggt
     5161 gcaacacaca ccaacacgac gttgatcctg gcgacgccca acgcttatcc gaaaacctaa
     5221 aaatcatgca aaaggagcta caaaagatat ggcaacagtt aaagtaagcc gaaccacttc
     5281 ttgtagccga gcgatcaatt acgccgaacc acgagcaacc gtcaaaacag ggataaactg
     5341 tgacattgat tatgccaaaa gcgaaatgaa acaaattcgc atgctgtatg gaaaagacga
     5401 tcgtgtacaa gcacacttat tgatccagtc ttttcgacca ggagaaatta ccgctgaaaa
     5461 agccaatcaa ctaggggcag agtatgcgaa aaaaatagcc cctgaccacc aaatcgcgat
     5521 ttacacacat acagacaaag accatattca taaccacatt gtcattaact ccgtcaatct
     5581 ggaaactggg aacaaatttc aagcccatgg tcaagcattt ttaaacaaat gttatgacct
     5641 aaatgacgag atttgtcttg aacatgactt aacaatcact gaacgaggga agaaacccga
     5701 aaaacgaacc atgtctgaaa tcaaattaaa agaaaaaaat gaacctgttt ggaaagacga
     5761 aattcggttt gcgattgatc aaacaatgaa aaacaaaaaa actcgaacgt ataaccaatt
     5821 ttgtgattct cttaaaacat tcggcattca ttgcttgaat cgtgggaaaa attttactta
     5881 cgaattgatc cacaaaaaga aaaaagtacg atccaacaaa ctaggaaagg attacgaaaa
     5941 ggagacgatt ttacatgagc tggacagacg agaacgagcc tacaatcaac aacgaaccaa
     6001 cgagctacga agagctcgag ctagcattcg cgaaactgca aaagaagtac gaagagaaca
     6061 acgaactctt acgactgaac aaccggaaat tagaccgact cgaagaaacc atgaccaaca
     6121 cgttgaaaga ggagcagact accaacaaga attatcgttc taaaacgtta caagacatag
     6181 aaaatgaaat aaagaaacaa cgtgcttttc ttgcacctta taccaaagaa agaatacaaa
     6241 ctacaattga ttcacaagtc aaaaaattcc accatgcgct attaaaccag cgaaataaag
     6301 aacagaaaaa agtaattatg cagcgatcaa tggatattct tgccccctta tgttgcttaa
     6361 tcatgacact gttagccttt tggctggtat ggggagaata aatagaaatg gcattctatt
     6421 taccctatct actcattttt gccagtattt taggaattat ttggcttagc tataaggctt
     6481 atcaaacaag atattcatta aaaggctcta aaattcgatt taagaggttt ttattgccag
     6541 gggttatatt tacattatca atcgttttaa gtggtttatt cggcgtttta gagggaaata
     6601 aacgtatttc aagaagtatt ttattagggg atatcactca aaagtatgaa gcagcatgtg
     6661 ataaaaagaa aaaggagcaa gtgttaacac aaaaaatgga agagtttacc acttgttatg
     6721 aggaaatgaa cgatattttt gtgaatcaag aaaaacgttt gacggataaa aacatggaaa
     6781 aactcactcg tctttatcaa aacttgccag aaaaacagca aaaagaggtt caagacaact
     6841 atgagcgaac caaaaaagac gtgcaatatt ttaaagatac gcagacagaa gaagcttgtt
     6901 ataatttatt cagtgataca atcccttttt caactagtga acaggaacga aaagaacggc
     6961 aacaatcagt tacctacgaa cgttataaag cactgcttca gcaagcaacg aacatacaaa
     7021 atccaacgaa aaaagaaacg gcattaaatt atctaaaaag tgtcaaggaa tggttagatc
     7081 agcaacagca aaattgaatt tgatcggcaa tcatgttaaa ctaaacatgc aaatgatcaa
     7141 acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt ttcaggttga
     7201 tgtcgccttt attgatttta cttcttgttt cggttattca accaataact gaataaagag
     7261 acacaaactt ctataagaag ggggcaaatg aacaattcaa acattagata cacctccttc
     7321 cagtcgcaag actgtcggat aggcgacaag ttattatatc ttaaaaaatt aaacttgtgt
     7381 ggtgttttta agtatcgctt attttagggt tgttagggct tgccctgacc gcctgtaagg
     7441 cgctttaaag ctagataagg atttttatcc aagactgtct taaaacggct tagataagca
     7501 ttcagaaccg ctcaaaggcc tctttaccaa catgttttta gcaaaggagc ctttaggcga
     7561 ctgcgcagta caatgtgaga gcgtagcgat cacacctttt tggcgaccgt aggaagcaag
     7621 gttttggatt ggcagttttg aaaaatgcgc gaacctgttt aaagaaaaaa gaagtggttt
     7681 ttaggcggga aaattagccc ccctcttttt atatctcttt ttaaacctct ttttaaacct
     7741 ctttaccgta agctgtgtcc ttactctacc aatggataca gaatgttatg aaggacattt
     7801 tgtctgttta tgaaggacat tttgtctgtt tatgaaggac attttgtctg tttatgaagg
     7861 acattttgtc tgtttgaacg tcaaatatga atgtcttgac atttaaaaaa tgtcattcat
     7921 aattggattg aggtgattat
//
