LOCUS       NZ_CP066650             6303 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 148 plasmid p148_6kb, complete
            sequence.
ACCESSION   NZ_CP066650
VERSION     NZ_CP066650.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038104
            Assembly: GCF_016415425.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 122.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:39:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,136
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 41 of 118
            Pseudo Genes (incomplete)         :: 71 of 118
            Pseudo Genes (internal stop)      :: 30 of 118
            Pseudo Genes (multiple problems)  :: 20 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066650.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 148"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p148_6kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(277..480)
                     /locus_tag="JCP76_RS15460"
                     /old_locus_tag="JCP76_15460"
     CDS             complement(277..480)
                     /locus_tag="JCP76_RS15460"
                     /old_locus_tag="JCP76_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(492..1025)
                     /locus_tag="JCP76_RS15465"
                     /old_locus_tag="JCP76_15465"
     CDS             complement(492..1025)
                     /locus_tag="JCP76_RS15465"
                     /old_locus_tag="JCP76_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(1475..1645)
                     /locus_tag="JCP76_RS15470"
                     /old_locus_tag="JCP76_15470"
     CDS             complement(1475..1645)
                     /locus_tag="JCP76_RS15470"
                     /old_locus_tag="JCP76_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1661..2194)
                     /locus_tag="JCP76_RS15475"
                     /old_locus_tag="JCP76_15475"
     CDS             complement(1661..2194)
                     /locus_tag="JCP76_RS15475"
                     /old_locus_tag="JCP76_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(2215..3165)
                     /locus_tag="JCP76_RS15480"
                     /old_locus_tag="JCP76_15480"
     CDS             complement(2215..3165)
                     /locus_tag="JCP76_RS15480"
                     /old_locus_tag="JCP76_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            3841..3927
                     /locus_tag="JCP76_RS15485"
                     /old_locus_tag="JCP76_15485"
     CDS             3841..3927
                     /locus_tag="JCP76_RS15485"
                     /old_locus_tag="JCP76_15485"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4048..4737)
                     /locus_tag="JCP76_RS15490"
                     /old_locus_tag="JCP76_15490"
     CDS             complement(4048..4737)
                     /locus_tag="JCP76_RS15490"
                     /old_locus_tag="JCP76_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4744..5022)
                     /locus_tag="JCP76_RS15495"
                     /old_locus_tag="JCP76_15495"
     CDS             complement(4744..5022)
                     /locus_tag="JCP76_RS15495"
                     /old_locus_tag="JCP76_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(4982..5896)
                     /locus_tag="JCP76_RS15500"
                     /old_locus_tag="JCP76_15500"
     CDS             complement(4982..5896)
                     /locus_tag="JCP76_RS15500"
                     /old_locus_tag="JCP76_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5878..6258)
                     /locus_tag="JCP76_RS15505"
                     /old_locus_tag="JCP76_15505"
     CDS             complement(5878..6258)
                     /locus_tag="JCP76_RS15505"
                     /old_locus_tag="JCP76_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2216..3164)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4986..5896)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accattgtac ctaaaaaggt acaatgagta aagctcacta aaagggtttg gcaagctcta
       61 tatttgctag ccaaatacct ccgtatttgg tgcaaataac cgcttgtcaa aggagcgcaa
      121 gctccccctt gaatccccct caaaatccac gctccacaat acaaaaaaag gctagcattt
      181 gctagccact ttttgtaaag tttcgcttat ttcaccgaag gtgaaattgt tgctttgtaa
      241 tattcaaaca accctattct tctaaaatgt atcgatttat ttttttttag actttattat
      301 gtctaagaca actaagatta acactaaaaa taaaccagat ttgtataatt gccctccgct
      361 tagcatatta acaattatag caacagctat agcaaatata aaaatacgaa tagtagctat
      421 ttgcggattc tttttagtaa atgtttttaa tgattttaat gatttagata tcaaaaacat
      481 cactccttaa cttataacgt tgaatatcta gtagctacaa ctcttccagc tgcatcatat
      541 cccctttggt aataaaatcc aatccagcca ttaccatttt tgtcccattc ttttgcaact
      601 gcattccctg gagaagcacc ttttttagtc caacttgcac ctgttaacaa agcaccaatt
      661 gcccaacctg ggataccaaa catagaagtg ctagctttcc ctcctgccca aaaggcagca
      721 ttaatagcat tagcagctac tccagtggtt acagcaatat ttgtatattc ctttttgccc
      781 catcttgcaa ctgcagctct tgtcatgata tctgaagaat ttgcaaaatc ttccgcacta
      841 taaccatcct catatttagt tacgaccata ccatcttcag tgtactcaat aactacattt
      901 tcagtagcat tgttttcgtt taatgatacc acttgggaat tttctgaagc ataaactata
      961 gtagttgacg gtaaaacaac tgccccaata gttaacaaag ctacccctaa agtaaatttt
     1021 ttcatagaat ttcctcctga tacagaaaaa tatatagaaa acgttttctt tgtgaaaatc
     1081 ataaaacaaa aggtataggc ttgtcaatat tttatttagt aaagataatt tatatttttg
     1141 ataatgtggt atagttagaa tgcattagga aaatatgtta tattaaattc ttgttgacat
     1201 aatgtacact atgccaagta gcccttgtat caaccgatac agaggctcac tttgtgattt
     1261 tcgattggtc taggtcaaaa gctcttttta agcttgattt tctgcggttg cttctttccg
     1321 tttctttgtg aatttttacc agtcattttt tactgggttg ttatttttaa taaaatatga
     1381 taaatttttg gtagcgcttc ataagcgcgt tgctttttca agaataaaca ctcattattt
     1441 tttaactggc tgctcgaaag agcagtcttt tttattattt atctgtttct tttacaaaca
     1501 attcttcgtt aggactgttg taataatttg gattcaattt caattcacca tgtttattcc
     1561 ctttattgat cactgacaat aacatcggac tgaatcctag aattcctagt aaaatagaac
     1621 cgaatagaag attttttctt gtcatgaatg ttccctccag ttatttccaa agttgccacc
     1681 attttttttc agtatcaatt tgtttgtcta ttgtttctgt tgcttcagaa agagtcgttg
     1741 agtcattctc tggagcttct tctgtataag tcaactgtag tttttcttgc agtttttcga
     1801 tttgtttgtt ggcttgtagg gtgagttgtt gttgctggtc gagtagtttt tgtatatgag
     1861 aattattttg ctctctgagg gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact
     1921 gatctagcaa cttgtttgta accacgtttt tgcttggtat attaacattt ttatcgttgt
     1981 cttgttcgta ctcgtgcaac ctatctagca acatttggtt gcctaagttg cgagcaagtt
     2041 gcaatactcc agaacgcaat acaaccttat aaccacgctc atttttagct atcatggaag
     2101 aatctaattt ttggatatgt ttcgatattg cttgcctact catcttaaaa agttctgaca
     2161 attcctttat tgtaaaggtt tcttgtggtt gcatactggt tgcctcctag gttgctactg
     2221 gaataaatta tctaaatcag ataataaact ttttttctct tttttcgaaa gtgcggactc
     2281 tgtttgtttt tcaagagctt ctttctcttt ttctagttgt ttgatttgtt ggaggagttc
     2341 aagttctcgt ttcttctgtt tgattttttc gttaattgtt gtagcaggtt cttcgtattc
     2401 tttttgttta tcagctaaag ccaatgcttt attttgttct tctttggttg gtgcatgttt
     2461 attaaaatca tctgcttttt tgatttctgg tttaaacgta aattgatagc caacgacagg
     2521 ttttcctcgc ccttttccgt atttcttttt gattgataat cctttgaaga ttgcggttaa
     2581 ttcctgtcgt ataggcttta taacacgatt atcaacgttt gcttggttgt tccagtaact
     2641 ttttggaata tctaatagtt catgaaaatc ctctttagaa aaataagcaa aacctttagt
     2701 tcgatattgt tttagtaatc gaaacatagt ctttgcatag ctactttgta attcgttaaa
     2761 ttgttggagt gaatagcgaa cccactcatc taaattgttt agtaaaggta aagctttttc
     2821 gtaaacttgt atgtctacat atggatcttt agcagttcca cgtattttaa actcactaaa
     2881 cataacgaat ctatctatat cgagcccatt agcacttctt ttaccaaaac gtaaatccat
     2941 taatttatca taagtcattt ctaaatcatt aatgaaacga ttattagcag ttgctttata
     3001 attacttaaa tcttttagtt ggtcaaaagt aagacggact gttttatcgc ctttttcgcg
     3061 cattctagac acaatagaga aaaatagatt catttcaact ggtgtaaatt tccgcaacgg
     3121 aatggtattt aattcatgat ggtatttcac gatttcattt gccaaagata ttcctcctta
     3181 ctccttcaat ggatacagac gttataaaca acgttttgtc tgtgataatg catattataa
     3241 catacgtgag tgcaaaaaaa gagtttaaaa gcagacaaaa tgtagtttaa aagcagacaa
     3301 aatgtagttt aaaagcagac aaaatgtagt ttaaaagcag acaaaatgta gtttataaaa
     3361 cgctgtatcc attgaaggag taaggacaca gccgacggta aagaggttta aaaaaaggtt
     3421 taaaaagaga ttataaaaga gggtgacaaa atttcccgcc taaaaaccac ttcttttttc
     3481 taccaacagg ttcgcgcatt tttcaaaact gccaattcaa aaccttgctc cctacggtcg
     3541 ctaaaaaagt gtgatcgcta tgctctcaca ttgtactgcg cagtcgccta aaggctcctt
     3601 tgctaaaaac aagtcagtaa cggaggtggg ggaagcctct gatagctcgt ctaagtcgtt
     3661 ttaagacagt cttgggtaaa aaaccttatc ttgttcttaa agcgccttac aggcggtcag
     3721 ggcaagccct aacaacccga aaataaggga tacttaaaaa caccacacag attttatttt
     3781 gtaaggtata ataacttgtc gcctatccga cagtcttgcg actggaagga ggtgtatcta
     3841 atgtttgaac ggttcatttg cccccttctt gtagaagttt gtgtctcttt attcagttat
     3901 tggttgaata accgaaacaa gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag
     3961 ccccagagaa tccgacctct ctggggcttt cgtgtatcta atgtttgatc atttgcaggt
     4021 tcagtttaac atgattgcta atcaaattca attttgttgt tgctgatcca accattcctt
     4081 gacactttct agataattta atgccgtttc ttttttcgtt ggattttgta tgtttgttgc
     4141 ttgctgaagc agtgtttcat aacgttcgta tgtgaccgat tgttgccgtt cttttcgttc
     4201 ttgttcactg gttgaaaaag ggattgtatc gctaaataag ttacaacaag cttcttctgt
     4261 ctgcgtatct ttaaaatatt gcacgtcttt ttttacttgc tcatattttt cttgtacctc
     4321 tttttgctgt ttttctggta agttttgata aaaacgagtg agttttttca tgtttttatc
     4381 tgttaaacgt ttctcttgat ccacaaaaat attgttcatg tcctcataac aagcggtaaa
     4441 ttcctccatt ttttgtgcta atgcttgctc ttttttcttt ttatcacatg ctgcttcata
     4501 cttttgagtg atatccccta ataaaatgct tcttgaaaca cgtttattac cctctaaaac
     4561 gccgaataaa ccacttaaaa cgattgataa ggcaaatata accccgacca ataaaaatcg
     4621 cttaaatcga attttagagc cttttaatga atatcttgtt tgataagcct tatagctaag
     4681 ccaaataatt cctaaaatac tggcagaaat gagtagatag ggtaaataga atgccatgtc
     4741 tgtttattct ccccatacca accaaaaggc taataatgtt agtactaagc aacataaggg
     4801 ggcaagaata tccatcgatc gttgaattaa agcctttttt tgttcttggt ttcgctggtc
     4861 taatagcgct tggtggagtt ttttgacttg tgaatccatc gtagtttgta tcctttcttg
     4921 ggtataaggc gcaagaaagg cacgttgtct ctttatttcg ttttcgatgt cttttaacgt
     4981 tttagagcga taattcttgt tggtggtctg ttcctcgttc aacgtgttgt tcatggtttc
     5041 ttcgagtcgg tctaatttcc gattgttcag gcgtaaaagt tcgttgttct cttcgtactt
     5101 cttctgcagt ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt cgttgttgat
     5161 tgtaggttcg ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg taatcctttc
     5221 ctagtttgtt ggatcgtact tttttctttt tgtggaccaa ttcgtaagta aaattttttc
     5281 cgcgattcaa gcaatgaatg ccgaatgttt taagtgaatc acaaaattgg gtatacgttc
     5341 gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct ttccaaaccg
     5401 gttcattttt ttcttttaat ttaatttcag acatggttcg tttttcggtt ttctttcctc
     5461 gttccgtgat tgttaagtca tgttcaagac aaatctcgtc atttaggtca taacatttgt
     5521 ttaaaaatgc ttgcccatgg gcttgaaatt ttttcccagt ttcaagatta acggagttaa
     5581 tgacaatgtg attatgaata tggtctttgt ctgtatgtgt gtaaatcgcg atttggtggt
     5641 taggggctat ttttttcgca tactctgccc ctagttgatt ggctttttca gcggtaattt
     5701 ctcctggtcg aaaggactga atcaataagt gtgcttgtac acgatcgtct tttccataaa
     5761 gcgtgcgtat ttgtttcatt tcacttttgg cataatcaat gtcacagttt attcctgttt
     5821 tgacggttgc tcgtggttct gcgtaattga ttgctcggct acatgaagtg gttcgactta
     5881 ctttaactgt tgccatatct tttgtagctc cttttgcatg atttttaggt tttcggataa
     5941 acgttgagca tcatcaggag caacgtcgtg ttggtgtgta ttgcaccact tggcaatttg
     6001 attgatattc cctccagctt tggctaaatc gcgcgcgatt gcctgggcaa tgtctggcgc
     6061 cactttcggt gtcaccagac gcgcgttttg tgccttggct ttcacgaaag ccggcacact
     6121 catttgcaaa gcttcagctg actgcttcag ctttgcaaat tcggattcgc tcactcgaaa
     6181 actgatttgt tttggctcgg atcgataggt atatttttta gaatgatcgc tagccaaatt
     6241 ttgatgttgt tcgctcataa gtcccaccct ttcagtacgt cctacggacg tatcgttcgc
     6301 aat
//
