LOCUS       NZ_CP066633             6311 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 137 plasmid p4, complete
            sequence.
ACCESSION   NZ_CP066633
VERSION     NZ_CP066633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038102
            Assembly: GCF_016415445.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 258.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:51:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 40 of 113
            Pseudo Genes (incomplete)         :: 69 of 113
            Pseudo Genes (internal stop)      :: 24 of 113
            Pseudo Genes (multiple problems)  :: 17 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066633.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 137"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(122..292)
                     /locus_tag="JCP61_RS15210"
                     /old_locus_tag="JCP61_15210"
     CDS             complement(122..292)
                     /locus_tag="JCP61_RS15210"
                     /old_locus_tag="JCP61_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(308..841)
                     /locus_tag="JCP61_RS15215"
                     /old_locus_tag="JCP61_15215"
     CDS             complement(308..841)
                     /locus_tag="JCP61_RS15215"
                     /old_locus_tag="JCP61_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(862..1812)
                     /locus_tag="JCP61_RS15220"
                     /old_locus_tag="JCP61_15220"
     CDS             complement(862..1812)
                     /locus_tag="JCP61_RS15220"
                     /old_locus_tag="JCP61_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            2487..2573
                     /locus_tag="JCP61_RS15225"
                     /old_locus_tag="JCP61_15225"
     CDS             2487..2573
                     /locus_tag="JCP61_RS15225"
                     /old_locus_tag="JCP61_15225"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2694..3383)
                     /locus_tag="JCP61_RS15230"
                     /old_locus_tag="JCP61_15230"
     CDS             complement(2694..3383)
                     /locus_tag="JCP61_RS15230"
                     /old_locus_tag="JCP61_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3390..3668)
                     /locus_tag="JCP61_RS15450"
     CDS             complement(3390..3668)
                     /locus_tag="JCP61_RS15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(3628..4542)
                     /locus_tag="JCP61_RS15235"
                     /old_locus_tag="JCP61_15235"
     CDS             complement(3628..4542)
                     /locus_tag="JCP61_RS15235"
                     /old_locus_tag="JCP61_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4524..4904)
                     /locus_tag="JCP61_RS15240"
                     /old_locus_tag="JCP61_15240"
     CDS             complement(4524..4904)
                     /locus_tag="JCP61_RS15240"
                     /old_locus_tag="JCP61_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5235..5438)
                     /locus_tag="JCP61_RS15245"
                     /old_locus_tag="JCP61_15245"
     CDS             complement(5235..5438)
                     /locus_tag="JCP61_RS15245"
                     /old_locus_tag="JCP61_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(5450..5983)
                     /locus_tag="JCP61_RS15250"
                     /old_locus_tag="JCP61_15250"
     CDS             complement(5450..5983)
                     /locus_tag="JCP61_RS15250"
                     /old_locus_tag="JCP61_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     Typing          complement(863..1811)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3632..4542)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgggttgtta tttttaataa aatatgataa atttttggta gcgcttcata agcgcgttgc
       61 tttttcaaga ataaacactc attatttttt aactggctgc tcgaaagagc agtctttttt
      121 attatttatc tgtttctttt acaaacaatt cttcgttagg actgttgtaa taatttggat
      181 tcaatttcaa ttcaccatgt ttattccctt tattgatcac tgacaataac atcggactga
      241 atcctagaat tcctagtaaa atagaaccga atagaagatt ttttcttgtc atgaatgttc
      301 cctccagtta tttccaaagt tgccaccatt ttttttcagt atcaatttgt ttgtctattg
      361 tttctgttgc ttcagaaaga gtcgttgagt cattctctgg agcttcttct gtataagtca
      421 actgtagttt ttcttgcagt ttttcgattt gtttgttggc ttgtagggtg agttgttgtt
      481 gctggtcgag tagtttttgt atatgagaat tattttgctc tctgagggca agttgttctc
      541 gtaaaaattc attttcttca cgcaactgat ctagcaactt gtttgtaacc acgtttttgc
      601 ttggtatatt aacattttta tcgttgtctt gttcgtactc gtgcaaccta tctagcaaca
      661 tttggttgcc taagttgcga gcaagttgca atactccaga acgcaataca accttataac
      721 cacgctcatt tttagctatc atggaagaat ctaatttttg gatatgtttc gatattgctt
      781 gcctactcat cttaaaaagt tctgacaatt cctttattgt aaaggtttct tgtggttgca
      841 tactggttgc ctcctaggtt gctactggaa taaattatct aaatcagata ataaactttt
      901 tttctctttt ttcgaaagtg cggactctgt ttgtttttca agagcttctt tctctttttc
      961 tagttgtttg atttgttgga ggagttcaag ttctcgtttc ttctgtttga ttttttcgtt
     1021 aattgttgta gcaggttctt cgtattcttt ttgtttatca gctaaagcca atgctttatt
     1081 ttgttcttct ttggttggtg catgtttatt aaaatcatct gcttttttga tttctggttt
     1141 aaacgtaaat tgatagccaa cgacaggttt tcctcgccct tttccgtatt tctttttgat
     1201 tgataatcct ttgaagattg cggttaattc ctgtcgtata ggctttataa cacgattatc
     1261 aacgtttgct tggttgttcc agtaactttt tggaatatct aatagttcat gaaaatcctc
     1321 tttagaaaaa taagcaaaac ctttagttcg atattgtttt agtaatcgaa acatagtctt
     1381 tgcatagcta ctttgtaatt cgttaaattg ttggagtgaa tagcgaaccc actcatctaa
     1441 attgtttagt aaaggtaaag ctttttcgta aacttgtatg tctacatatg gatctttagc
     1501 agttccacgt attttaaact cactaaacat aacgaatcta tctatatcga gcccattagc
     1561 acttctttta ccaaaacgta aatccattaa tttatcataa gtcatttcta aatcattaat
     1621 gaaacgatta ttagcagttg ctttataatt acttaaatct tttagttggt caaaagtaag
     1681 acggactgtt ttatcgcctt tttcgcgcat tctagacaca atagagaaaa atagattcat
     1741 ttcaactggt gtaaatttcc gcaacggaat ggtatttaat tcatgatggt atttcacgat
     1801 ttcatttgcc aaagatattc ctccttactc cttcaatgga tacagacgtt ataaacaacg
     1861 ttttgtctgt gataatgcat attataacat acgtgagtgc aaaaaaagag tttaaaagca
     1921 gacaaaatgt agtttaaaag cagacaaaat gtagtttaaa agcagacaaa atgtagttta
     1981 aaagcagaca aaatgtagtt tataaaacgc tgtatccatt gaaggagtaa ggacacagct
     2041 tacggtaaag aggtttaaaa agaggtttaa aaagagatat aaaaagaggg gggctaattt
     2101 tcccgcctaa aaaccacttc ttttttcttt aaacaggttc gcgcattttt caaaactgcc
     2161 aatccaaaac cttgcttcct acggtcgcca aaaaggtgtg atcgctacgc tctcacattg
     2221 tactgcgcag tcgcctaaag gctcctttgc taaaaacatg ttggtaaaga ggcctttgag
     2281 cggttctgaa tgcttatcta agccgtttta agacagtctt ggataaaaat ccttatctag
     2341 ctttaaagcg ccttacaggc ggtcagggca agccctaaca accctaaaat aagcgatact
     2401 taaaaacacc acacaagttt aattttttaa gatataataa cttgtcgcct atccgacagt
     2461 cttgcgactg gaaggaggtg tatctaatgt ttgaattgtt catttgcccc cttcttatag
     2521 aagtttgtgt ctctttattc agttattggt tgaataaccg aaacaagaag taaaatcaat
     2581 aaaggcgaca tcaacctgaa aaaaagcccc agagaatccg acctctctgg ggctttcgtg
     2641 tatctaatgt ttgatcattt gcatgtttag tttaacatga ttgccgatca aattcaattt
     2701 tgctgttgct gatctaacca ttccttgaca ctttttagat aatttaatgc cgtttctttt
     2761 ttcgttggat tttgtatgtt cgttgcttgc tgaagcagtg ctttataacg ttcgtaggta
     2821 actgattgtt gccgttcttt tcgttcctgt tcactagttg aaaaagggat tgtatcactg
     2881 aataaattat aacaagcttc ttctgtctgc gtatctttaa aatattgcac gtcttttttg
     2941 gttcgctcat agttgtcttg aacctctttt tgctgttttt ctggcaagtt ttgataaaga
     3001 cgagtgagtt tttccatgtt tttatccgtc aaacgttttt cttgattcac aaaaatatcg
     3061 ttcatttcct cataacaagt ggtaaactct tccatttttt gtgttaacac ttgctccttt
     3121 ttctttttat cacatgctgc ttcatacttt tgagtgatat cccctaataa aatacttctt
     3181 gaaatacgtt tatttccctc taaaacgccg aataaaccac ttaaaacgat tgataatgta
     3241 aatataaccc ctggcaataa aaacctctta aatcgaattt tagagccttt taatgaatat
     3301 cttgtttgat aagccttata gctaagccaa ataattccta aaatactggc aaaaatgagt
     3361 agatagggta aatagaatgc catttctatt tattctcccc ataccagcca aaaggctaac
     3421 agtgtcatga ttaagcaaca taagggggca agaatatcca ttgatcgctg cataattact
     3481 tttttctgtt ctttatttcg ctggtttaat agcgcatggt ggaatttttt gacttgtgaa
     3541 tcaattgtag tttgtattct ttctttggta taaggtgcaa gaaaagcacg ttgtttcttt
     3601 atttcatttt ctatgtcttg taacgtttta gaacgataat tcttgttggt agtctgctcc
     3661 tctttcaacg tgttggtcat ggtttcttcg agtcggtcta atttccggtt gttcagtcgt
     3721 aagagttcgt tgttctcttc gtacttcttt tgcagtttcg cgaatgctag ctcgagctct
     3781 tcgtagctcg ttggttcgtt gttgattgta ggctcgttct cgtctgtcca gctcatgtaa
     3841 aatcgtctcc ttttcgtaat cctttcctag tttgttggat cgtacttttt tctttttgtg
     3901 gatcaattcg taagtaaaat ttttcccacg attcaagcaa tgaatgccga atgttttaag
     3961 agaatcacaa aattggttat acgttcgagt ttttttgttt ttcattgttt gatcaatcgc
     4021 aaaccgaatt tcgtctttcc aaacaggttc atttttttct tttaatttga tttcagacat
     4081 ggttcgtttt tcgggtttct tccctcgttc agtgattgtt aagtcatgtt caagacaaat
     4141 ctcgtcattt aggtcataac atttgtttaa aaatgcttga ccatgggctt gaaatttgtt
     4201 cccagtttcc agattgacgg agttaatgac aatgtggtta tgaatatggt ctttgtctgt
     4261 atgtgtgtaa atcgcgattt ggtggtcagg ggctattttt ttcgcatact ctgcccctag
     4321 ttgattggct ttttcagcgg taatttctcc tggtcgaaaa gactggatca ataagtgtgc
     4381 ttgtacacga tcgtcttttc catacagcat gcgaatttgt ttcatttcgc ttttggcata
     4441 atcaatgtca cagtttatcc ctgttttgac ggttgctcgt ggttcggcgt aattgatcgc
     4501 tcggctacaa gaagtggttc ggcttacttt aactgttgcc atatcttttg tagctccttt
     4561 tgcatgattt ttaggttttc ggataagcgt tgggcgtcgc caggatcaac gtcgtgttgg
     4621 tgtgtgttgc accacttggc aatttgattg atattcccgc cagctttggc taaatcgcgc
     4681 gcgattgcct gagcaatgtc tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc
     4741 ttggctttca cgaaagccgg cacactcatt tgcaaagctt cagctgactg cttcagcttt
     4801 gcaaattcgg attcgctcac tcgaaaactg atttgttttg gctcggatcg ataggtatat
     4861 tttttagaat gattgctagc caaatattga ttttgttcgc tcataagtcc caccctttca
     4921 gtacgtccta cggacgtatc gttcgcaata ccattgtacc taaaaaggta caataagtaa
     4981 agctcactaa agctcactaa agggcttggc aagctctata tttgctagcc aaatacctcc
     5041 gtatttggtg caaataaccg cttgtcaaag gagcgcaagc tcccccttga atccccctca
     5101 aaatccacgc tccacaatac aaaaaaaggc tagcatttgc tagccacttt ttgtaaagtt
     5161 tcgcttattt caccgaaggt gaaattgttg ctttgtaata ttcaaacaac cctattcttc
     5221 taaaatgtat cgatttattt ttttttagac tttattatgt ctaagacaac taagattaac
     5281 actaaaaata aaccagattt gtataattgc cctccgctta gcatattaac aattatagca
     5341 acagctatag caaatataaa aatacgaata gtagctattt gcggattctt tttagtaaat
     5401 gtttttaatg attttaatga tttagatatc aaaaacatca ctccttaact tataacgttg
     5461 aatatctagt agctacaact cttccagctg catcatatcc cctttggtaa taaaatccaa
     5521 tccagccatt accatttttg tcccattctt ttgcaactgc attccctgga gaagcacctt
     5581 ttttagtcca acttgcacct gttaacaaag caccaattgc ccaacctggg ataccaaaca
     5641 tagaagtgct agctttccct cctgcccaaa aggcagcatt aatagcatta gcagctactc
     5701 cagtggttac agcaatattt gtatattcct ttttgcccca tcttgcaact gcagctcttg
     5761 tcatgatatc tgaagaattt gcaaaatctt ccgcactata accatcctca tatttagtta
     5821 cgaccatacc atcttcagtg tactcaataa ctacattttc agtagcattg ttttcgttta
     5881 atgataccac ttgggaattt tctgaagcat aaactatagt agttgacggt aaaacaactg
     5941 ccccaatagt taacaaagct acccctaaag taaatttttt catagaattt cctcctgata
     6001 cagaaaaata tatagaaaac gttttctttg tgaaaatcat aaaacaaaag gtataggctt
     6061 gtcaatattt tatttagtaa agataattta tatttttgat aatgtggtat agttagaatg
     6121 cattaggaaa atatgttata ttaaattctt gttgacataa tgtacactat gccaagtagc
     6181 ccttgtatca accgatacag aggctcactt tgtgattttc gattggtcta ggtcaaaagc
     6241 tctttttaag cttgattttc tgcggttgct tctttccgtt tctttgtgaa tttttaccag
     6301 tcatttttta c
//
