LOCUS       NZ_CP066602             6303 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 111 plasmid p111_6kb, complete
            sequence.
ACCESSION   NZ_CP066602
VERSION     NZ_CP066602.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038095
            Assembly: GCF_016415345.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:48:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,955
            Genes (coding)                    :: 2,822
            CDSs (with protein)               :: 2,822
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 63 of 133
            Pseudo Genes (incomplete)         :: 74 of 133
            Pseudo Genes (internal stop)      :: 26 of 133
            Pseudo Genes (multiple problems)  :: 27 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066602.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 111"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p111_6kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(197..400)
                     /locus_tag="JCP62_RS15005"
                     /old_locus_tag="JCP62_15010"
     CDS             complement(197..400)
                     /locus_tag="JCP62_RS15005"
                     /old_locus_tag="JCP62_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(412..945)
                     /locus_tag="JCP62_RS15010"
                     /old_locus_tag="JCP62_15015"
     CDS             complement(412..945)
                     /locus_tag="JCP62_RS15010"
                     /old_locus_tag="JCP62_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(1395..1565)
                     /locus_tag="JCP62_RS15015"
                     /old_locus_tag="JCP62_15020"
     CDS             complement(1395..1565)
                     /locus_tag="JCP62_RS15015"
                     /old_locus_tag="JCP62_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1581..2114)
                     /locus_tag="JCP62_RS15020"
                     /old_locus_tag="JCP62_15025"
     CDS             complement(1581..2114)
                     /locus_tag="JCP62_RS15020"
                     /old_locus_tag="JCP62_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(2135..3085)
                     /locus_tag="JCP62_RS15025"
                     /old_locus_tag="JCP62_15030"
     CDS             complement(2135..3085)
                     /locus_tag="JCP62_RS15025"
                     /old_locus_tag="JCP62_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            3761..3847
                     /locus_tag="JCP62_RS15030"
                     /old_locus_tag="JCP62_15035"
     CDS             3761..3847
                     /locus_tag="JCP62_RS15030"
                     /old_locus_tag="JCP62_15035"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3968..4657)
                     /locus_tag="JCP62_RS15035"
                     /old_locus_tag="JCP62_15040"
     CDS             complement(3968..4657)
                     /locus_tag="JCP62_RS15035"
                     /old_locus_tag="JCP62_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4902..5816)
                     /locus_tag="JCP62_RS15040"
                     /old_locus_tag="JCP62_15045"
     CDS             complement(4902..5816)
                     /locus_tag="JCP62_RS15040"
                     /old_locus_tag="JCP62_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5798..6178)
                     /locus_tag="JCP62_RS15045"
                     /old_locus_tag="JCP62_15050"
     CDS             complement(5798..6178)
                     /locus_tag="JCP62_RS15045"
                     /old_locus_tag="JCP62_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2136..3084)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4906..5816)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgtatttgg tgcaaataac cgcttgtcaa aggagcgcaa gctccccctt gaatccccct
       61 caaaatccac gctccacaat acaaaaaaag gctagcattt gctagccact ttttgtaaag
      121 tttcgcttat ttcaccgaag gtgaaattgt tgctttgtaa tattcaaaca accctattct
      181 tctaaaatgt atcgatttat ttttttttag actttattat gtctaagaca actaagatta
      241 acactaaaaa taaaccagat ttgtataatt gccctccgct tagcatatta acaattatag
      301 caacagctat agcaaatata aaaatacgaa tagtagctat ttgcggattc tttttagtaa
      361 atgtttttaa tgattttaat gatttagata tcaaaaacat cactccttaa cttataacgt
      421 tgaatatcta gtagctacaa ctcttccagc tgcatcatat cccctttggt aataaaatcc
      481 aatccagcca ttaccatttt tgtcccattc ttttgcaact gcattccctg gagaagcacc
      541 ttttttagtc caacttgcac ctgttaacaa agcaccaatt gcccaacctg ggataccaaa
      601 catagaagtg ctagctttcc ctcctgccca aaaggcagca ttaatagcat tagcagctac
      661 tccagtggtt acagcaatat ttgtatattc ctttttgccc catcttgcaa ctgcagctct
      721 tgtcatgata tctgaagaat ttgcaaaatc ttccgcacta taaccatcct catatttagt
      781 tacgaccata ccatcttcag tgtactcaat aactacattt tcagtagcat tgttttcgtt
      841 taatgatacc acttgggaat tttctgaagc ataaactata gtagttgacg gtaaaacaac
      901 tgccccaata gttaacaaag ctacccctaa agtaaatttt ttcatagaat ttcctcctga
      961 tacagaaaaa tatatagaaa acgttttctt tgtgaaaatc ataaaacaaa aggtataggc
     1021 ttgtcaatat tttatttagt aaagataatt tatatttttg ataatgtggt atagttagaa
     1081 tgcattagga aaatatgtta tattaaattc ttgttgacat aatgtgtact atgccaagta
     1141 gcccttgtat caaccgatac agaggctcac tttgtgattt tcgattggtc taggtcaaaa
     1201 gctcttttta agcttgattt tctgcggttg cttctttccg tttctttgtg aatttttacc
     1261 agtcattttt tactgggttg ttatttttaa taaaatatga taaatttttg gtagcgcttc
     1321 ataagcgcgt tgctttttca agaataaaca ctcattattt tttaactggc tgctcgaaag
     1381 agcagtcttt tttattattt atctgtttct tttacaaaca attcttcgtt aggactgttg
     1441 taataatttg gattcaattt caattcacca tgtttattcc ctttattgat cactgacaat
     1501 aacatcggac tgaatcctag aattcctagt aaaatagaac cgaatagaag attttttctt
     1561 gtcatgaatg ttccctccag ttatttccaa agttgccacc attttttttc agtatcaatt
     1621 tgtttgtcta ttgtttctgt tgcttcagaa agagtcgttg agtcattctc tggagcttct
     1681 tctgtataag tcaactgtag tttttcttgc agtttttcga tttgtttgtt ggcttgtagg
     1741 gtgagttgtt gttgctggtc gagtagtttt tgtatatgag aattattttg ctctctgagg
     1801 gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact gatctagcaa cttgtttgta
     1861 accacgtttt tgcttggtat attaacattt ttatcgttgt cttgttcgta ctcgtgcaac
     1921 ctatctagca acatttggtt gcctaagttg cgagcaagtt gcaatactcc agaacgcaat
     1981 acaaccttat aaccacgctc atttttagct atcatggaag aatctaattt ttggatatgt
     2041 ttcgatattg cttgcctact catcttaaaa agttctgaca attcctttat tgtaaaggtt
     2101 tcttgtggtt gcatactggt tgcctcctag gttgctactg gaataaatta tctaaatcag
     2161 ataataaact ttttttctct tttttcgaaa gtgcggactc tgtttgtttt tcaagagctt
     2221 ctttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt
     2281 tgattttttc gttaattgtt gtagcaggtt cttcgtattc tttttgttta tcagctaaag
     2341 ccaatgcttt attttgttct tctttggttg gtgcatgttt attaaaatca tctgcttttt
     2401 tgatttctgg tttaaacgta aattgatagc caacgacagg ttttcctcgc ccttttccgt
     2461 atttcttttt gattgataat cctttgaaga ttgcggttaa ttcctgtcgt ataggcttta
     2521 taacacgatt atcaacgttt gcttggttgt tccagtaact ttttggaata tctaatagtt
     2581 catgaaaatc ctctttagaa aaataagcaa aacctttagt tcgatattgt tttagtaatc
     2641 gaaacatagt ctttgcatag ctactttgta attcgttaaa ttgttggagt gaatagcgaa
     2701 cccactcatc taaattgttt agtaaaggta aagctttttc gtaaacttgt atgtctacat
     2761 atggatcttt agcagttcca cgtattttaa actcactaaa cataacgaat ctatctatat
     2821 cgagcccatt agcacttctt ttaccaaaac gtaaatccat taatttatca taagtcattt
     2881 ctaaatcatt aatgaaacga ttattagcag ttgctttata attacttaaa tcttttagtt
     2941 ggtcaaaagt aagacggact gttttatcgc ctttttcgcg cattctagac acaatagaga
     3001 aaaatagatt catttcaact ggtgtaaatt tccgcaacgg aatggtattt aattcatgat
     3061 ggtatttcac gatttcattt gccaaagata ttcctcctta ctccttcaat ggatacagac
     3121 gttataaaca acgttttgtc tgtgataatg catattataa catacgtgag tgcaaaaaaa
     3181 gagtttaaaa gcagacaaaa tgtagtttaa aagcagacaa aatgtagttt aaaagcagac
     3241 aaaatgtagt ttaaaagcag acaaaatgta gtttataaaa cgctgtatcc attgaaggag
     3301 taaggacaca gccgacggta aagaggttta aaaaaaggtt taaaaagaga ttataaaaga
     3361 gggtgacaaa atttcccgcc taaaaaccac ttcttttttc taccaacagg ttcgcgcatt
     3421 tttcaaaact gccaattcaa aaccttgctc cctacggtcg ctaaaaaagt gtgatcgcta
     3481 tgctctcaca ttgtactgcg cagtcgccta aaggctcctt tgctaaaaac aagtcagtaa
     3541 cggaggtggg ggaagcctct gatagctcgt ctaagtcgtt ttaagacagt cttgggtaaa
     3601 aaaccttatc ttgttcttaa agcgccttac aggcggtcag ggcaagccct aacaacccga
     3661 aaataaggga tacttaaaaa caccacacag attttatttt gtaaggtata ataacttgtc
     3721 gcctatccga cagtcttgcg actggaagga ggtgtatcta atgtttgaac ggttcatttg
     3781 cccccttctt gtagaagttt gtgtctcttt attcagttat tggttgaata accgaaacaa
     3841 gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag ccccagagaa tccgacctct
     3901 ctggggcttt cgtgtatcta atgtttgatc atttgcaggt tcagtttaac atgattgcta
     3961 atcaaattca attttgttgt tgctgatcca accattcctt gacactttct agataattta
     4021 atgccgtttc ttttttcgtt ggattttgta tgtttgttgc ttgctgaagc agtgtttcat
     4081 aacgttcgta tgtgaccgat tgttgccgtt cttttcgttc ttgttcactg gttgaaaaag
     4141 ggattgtatc gctaaataag ttacaacaag cttcttctgt ctgcgtatct ttaaaatatt
     4201 gcacgtcttt ttttacttgc tcatattttt cttgtacctc tttttgctgt ttttctggta
     4261 agttttgata aaaacgagtg agttttttca tgtttttatc tgttaaacgt ttctcttgat
     4321 ccacaaaaat attgttcatg tcctcataac aagcggtaaa ttcctccatt ttttgtgcta
     4381 atgcttgctc ttttttcttt ttatcacatg ctgcttcata cttttgagtg atatccccta
     4441 ataaaatgct tcttgaaaca cgtttattac cctctaaaac gccgaataaa ccacttaaaa
     4501 cgattgataa ggcaaatata accccgacca ataaaaatcg cttaaatcga attttagagc
     4561 cttttaatga atatcttgtt tgataagcct tatagctaag ccaaataatt cctaaaatac
     4621 tggcagaaat gagtagatag ggtaaataga atgccatgtc tgtttattct ccccatacca
     4681 accaaaaggc taataatgtt agtactaagc aacataaggg ggcaagaata tccatcgatc
     4741 gttgaattaa agcctttttt tgttcttggt ttcgctggtc taatagcgct tggtggagtt
     4801 ttttgacttg tgaatccatc gtagtttgta tcctttcttg ggtataaggc gcaagaaagg
     4861 cacgttgtct ctttatttcg ttttcgatgt cttttaacgt tttagagcga taattcttgt
     4921 tggtggtctg ttcctcgttc aacgtgttgt tcatggtttc ttcgagtcgg tctaatttcc
     4981 gattgttcag gcgtaaaagt tcgttgttct cttcgtactt cttctgcagt ttcgcgaatg
     5041 ctagctcgag ctcttcgtag ctcgttggtt cgttgttgat tgtaggttcg ttctcgtctg
     5101 tccagctcat gtaaaatcgt ctccttttcg taatcctttc ctagtttgtt ggatcgtact
     5161 tttttctttt tgtggaccaa ttcgtaagta aaattttttc cgcgattcaa gcaatgaatg
     5221 ccgaatgttt taagtgaatc acaaaattgg gtatacgttc gagttttttt gtttttcatt
     5281 gtttgatcaa tcgcaaaccg aatttcgtct ttccaaaccg gttcattttt ttcttttaat
     5341 ttaatttcag acatggttcg tttttcggtt ttctttcctc gttccgtgat tgttaagtca
     5401 tgttcaagac aaatctcgtc atttaggtca taacatttgt ttaaaaatgc ttgcccatgg
     5461 gcttgaaatt ttttcccagt ttcaagatta acggagttaa tgacaatgtg attatgaata
     5521 tggtctttgt ctgtatgtgt gtaaatcgcg atttggtggt taggggctat ttttttcgca
     5581 tactctgccc ctagttgatt ggctttttca gcggtaattt ctcctggtcg aaaggactga
     5641 atcaataagt gtgcttgtac acgatcgtct tttccataaa gcgtgcgtat ttgtttcatt
     5701 tcacttttgg cataatcaat gtcacagttt attcctgttt tgacggttgc tcgtggttct
     5761 gcgtaattga ttgctcggct acatgaagtg gttcgactta ctttaactgt tgccatatct
     5821 tttgtagctc cttttgcatg atttttaggt tttcggataa acgttgagca tcatcaggag
     5881 caacgtcgtg ttggtgtgta ttgcaccact tggcaatttg attgatattc cctccagctt
     5941 tggctaaatc gcgcgcgatt gcctgggcaa tgtctggcgc cactttcggt gtcaccagac
     6001 gcgcgttttg tgccttggct ttcacgaaag ccggcacact catttgcaaa gcttcagctg
     6061 actgcttcag ctttgcaaat tcggattcgc tcactcgaaa actgatttgt tttggctcgg
     6121 atcgataggt atatttttta gaatgatcgc tagccaaatt ttgatgttgt tcgctcataa
     6181 gtcccaccct ttcagtacgt cctacggacg tatcgttcgc aataccattg tacctaaaaa
     6241 ggtacaatga gtaaagctca ctaaaagggt ttggcaagct ctatatttgc tagccaaata
     6301 cct
//
