LOCUS       NZ_CP066601             7165 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 111 plasmid p111_7kb, complete
            sequence.
ACCESSION   NZ_CP066601
VERSION     NZ_CP066601.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038095
            Assembly: GCF_016415345.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:48:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,955
            Genes (coding)                    :: 2,822
            CDSs (with protein)               :: 2,822
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 63 of 133
            Pseudo Genes (incomplete)         :: 74 of 133
            Pseudo Genes (internal stop)      :: 26 of 133
            Pseudo Genes (multiple problems)  :: 27 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066601.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 111"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p111_7kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(179..622)
                     /locus_tag="JCP62_RS14960"
                     /old_locus_tag="JCP62_14965"
     CDS             complement(179..622)
                     /locus_tag="JCP62_RS14960"
                     /old_locus_tag="JCP62_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(595..1749)
                     /locus_tag="JCP62_RS14965"
                     /old_locus_tag="JCP62_14970"
     CDS             complement(595..1749)
                     /locus_tag="JCP62_RS14965"
                     /old_locus_tag="JCP62_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_044383358.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            complement(2160..2612)
                     /locus_tag="JCP62_RS14970"
                     /old_locus_tag="JCP62_14975"
     CDS             complement(2160..2612)
                     /locus_tag="JCP62_RS14970"
                     /old_locus_tag="JCP62_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025089292.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKVMLKNHTKTLILLFHFVIFLSLVFTLIQSKFFYFSHS
                     IATWLLIFFLSLLLVINTLIIIFNKHKNLFLHSVFPIILLSFLYFV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(2696..3217)
                     /locus_tag="JCP62_RS14975"
                     /old_locus_tag="JCP62_14980"
     CDS             complement(2696..3217)
                     /locus_tag="JCP62_RS14975"
                     /old_locus_tag="JCP62_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_031592680.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENNNAI
                     TTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            complement(3246..3983)
                     /locus_tag="JCP62_RS14980"
                     /old_locus_tag="JCP62_14985"
     CDS             complement(3246..3983)
                     /locus_tag="JCP62_RS14980"
                     /old_locus_tag="JCP62_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            4628..4714
                     /locus_tag="JCP62_RS14985"
                     /old_locus_tag="JCP62_14990"
     CDS             4628..4714
                     /locus_tag="JCP62_RS14985"
                     /old_locus_tag="JCP62_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_109746925.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4835..5524)
                     /locus_tag="JCP62_RS14990"
                     /old_locus_tag="JCP62_14995"
     CDS             complement(4835..5524)
                     /locus_tag="JCP62_RS14990"
                     /old_locus_tag="JCP62_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031592678.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     KEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(5526..5804)
                     /locus_tag="JCP62_RS15425"
     CDS             complement(5526..5804)
                     /locus_tag="JCP62_RS15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(5764..6678)
                     /locus_tag="JCP62_RS14995"
                     /old_locus_tag="JCP62_15000"
     CDS             complement(5764..6678)
                     /locus_tag="JCP62_RS14995"
                     /old_locus_tag="JCP62_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6660..7040)
                     /locus_tag="JCP62_RS15000"
                     /old_locus_tag="JCP62_15005"
     CDS             complement(6660..7040)
                     /locus_tag="JCP62_RS15000"
                     /old_locus_tag="JCP62_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_159287021.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3247..3983)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5768..6678)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgtatttgg ctagcaaata accgcttttc aaaggggcgc aagctccccc ttgaatcccc
       61 ctcaaaaccc acgctccaca atacaaaaaa ggctagcaaa tgctagccac tttttgtaaa
      121 gtttcgcttg tttcaccttc ggtgaaatta ttgttttgta atatcaaaca actattcatc
      181 aatcagcttt aaaaaattca attggtttag ctgtcccttc tgtcccatca ttcgttgtca
      241 aaataaatac gagctttttt ggagaatcca aactaaaagt gtaatctaat tgttttttat
      301 tagagcgcag tgttagtaaa ttatttgttg ctttgtaaat gaaaacttgt tcatttatat
      361 ttaaattttt atcaatgatt tgaattttat gattgttctc ttttactaag atggtatatt
      421 gttctatatt ttttgaagta tttaaattag tgattactag acgttctgaa tgatctttag
      481 tttgccaagt tcctagaaaa ggaatcagat cagtcttttg tatttcttgt ttttttgaac
      541 aactagttaa gaaaagaact aacactagac ccataactaa agcaatgggt ttatttacca
      601 atttgtaatg gtattgtccc aagtaaaacc acctccaggt acaggaattg taactggtcc
      661 tgcatttgca cttattacag ttgggtagtt ataccaagga ttccacacat agtaagtttg
      721 ataaccacct tgattgaccc acccaaataa tgcaagtgca tgacggctgt tactataatt
      781 tccataacca gcagttccca tataaatggc atttcctttt cttagttgtg tttggacctc
      841 tgaaaggttt agtattcgtg ctactttttt aggataaaca ccacagtaat ttgcatattt
      901 tattatttga tcttgattaa gactttttga ttcaatttga ctgttgttta aattaggata
      961 ggtataatgc ataatggctc tagctgaagt agcacgtaca tctgttttgt tacataagat
     1021 agcagcttct acgtaagcag cacaccacgg aaaacttgtt tgggtttctg ctatacgcca
     1081 attaatcata ataaagttat ctccggagga gacacttttt acacgtttaa ccgcaggaaa
     1141 attttgtttt ttagagaaat ttttaatttt attttttata tttacggata taagatcatt
     1201 tttttctgta aaggaagaaa caccattttt ttctgtactt aaaggtgatt tccaaagtag
     1261 tttaatttgt ccattatcgg aataataaat gctattatct tgaaaaaata aggttgtatc
     1321 tgttatatta ctatctacta gattttgaag ttcttttgct aaaaattgtg aaatacttcc
     1381 tgaatattct ccatttgtat tttttttgac ggtataaata tatttaatcg tattatcagc
     1441 gtctgaaaat actggaaaga aaatagaact atttgccgta ttttctccat cgagcatttt
     1501 aaatggcgaa ccaagagtat agttaatatc agtttcggtg ttattttcca ttaaaagaat
     1561 tgaagttatg gagtgttcaa aatcagtttt cgcaaaatta attgcttgtt cttcaattgg
     1621 ttgggagttg atattgaaat tagaattttc tgaagcatag gtatttgtaa ttcctatact
     1681 aaaaattcca atcatcaagg aaaagaggag acctagataa attttctttt tgggtttcaa
     1741 aataatcacg cctttgtgtt aaatttaagt ggtatacacc tataattaaa atatacaata
     1801 tgcgataaca aatttttgtt atagttcata aaatattgtt ttgggaacat aatcgttatt
     1861 tatagcgtta gttaacataa tgtcgactat gccaagtagc ccctctatca agggctatag
     1921 ctgctcactt tgtgattttt cattggtcta ggtcaaaagc tttttttaag gcttgatttt
     1981 ctgcagttgc ttctttccgt ttctttgtga atttttacca gtcatttttt actggattgt
     2041 tattttttat aaaatatgat aaaattttgg tagcgcttta taagcgcgtt gctttttcaa
     2101 gaataaactc tcattatttt tttaactggc tgctcgaaag agcagtcttt ttttatactt
     2161 tatacaaagt ataaaaaact cagcaaaata attggaaaaa cactatgaag aaataaattt
     2221 ttatgtttat taaaaataat aatcagagta ttgattacta atagcaaact tagaaaaaat
     2281 attaataacc aagtggcaat tgaatggcta aaataaaaga atttggactg aattaaggta
     2341 aaaactaaac ttaaaaaaat tacaaaatga aataatagta ttagtgtttt tgtatgattt
     2401 tttaacataa ctttttcttc tcctaacaaa atattgtcta gtgataaatc aaataactta
     2461 gcaaattgaa ttaaattttc tgtatctggt attgttctac cattttccca attagaaatg
     2521 gtttttttag atactaaaag catttcagct aaatcttctt gagttagttg tttttctatt
     2581 cttttttgtt tgattacttt acttatagtc atttgatcac ctaaacatat tttttatttt
     2641 tagaaagata aagtctatag aaattgattt cttctttatg gaaagttggt aattgttacc
     2701 aaaatttcca ccactttttt tctgttgtta ttgcattgtt gttttcttta ttgacagaag
     2761 ttaatacctc ttcttcaggt tcttccgtat aagctaattg taactgttct tgcagttttt
     2821 cgatctgttg atttgcctgc aaggtgagtt gttgttgctg gtcgagtaat ttttgcatat
     2881 tagaattatt ttgctctttg agagaaagtt gttctcgtaa aaattcattt tcttcacgca
     2941 acttatctag caactcgttt acaaccacgt tttttcttga tatataaacg tttttatcgt
     3001 tttcttgttc atactcatgc aacctatcta gtaacctttg gttacctaag ttgctagcaa
     3061 gttgcaatac tccagaacgt aatacaacct tataaccacg ctcattttta gctaataagg
     3121 acgggtctaa tttatcaaca tgtttagaaa ttgcttgcct acttatttca aataattcag
     3181 ataattcctt tattgtaaag gtttctttgg gttgcatgat aattgcctcc taaattgtta
     3241 ggttattatt tctctaacca attatctaag gtaattgaag gtatattttc ttcaaaataa
     3301 atttgcagag atcctatctt atttcctttg taggctttaa tttttttgat ttcaaaagtt
     3361 ttaaaaatag cagggtgagt tgaattaacg tttaaaaatt cttttttcgc ttgggcaaaa
     3421 atttgtttat caatatctct catgcgataa ctttctggta ttgctaatag agctctaaaa
     3481 tcttctattt ttacaaacca gtgacctttt ttagatcttc tgtctttatg agccataagt
     3541 tggcgatata actctttaac gtagctactc tttaagtttg tcatattttc taattcgaaa
     3601 cgagtaaaat tagttccgat tgaattaagt atatattcaa attcttcaga aacctttatt
     3661 aataatgttt cattcacttt atcgatttta aatttttgaa acagatttaa ttgttcatat
     3721 gttttttcgt cttcataaat aaaagacaaa gtaatgattt tttttgacat ttttattaaa
     3781 tcattagcaa aacggtcgcc acgtgcattt atattgtaat caattaattc ttttatttgt
     3841 ttaaatgtaa aaatttcttc ttttgtacct tttcttttca ttttagaaca tattccccaa
     3901 aataaatcca tatctatagg ggtaaatttt cttaaaggga cagaatttaa tccattatta
     3961 tatcgaacta tatcgttact caataaaaat cacctcaata aattatgtta tcatagacat
     4021 aagagggtgt aaaatacaat ttatccccca tagtgtgacg atttatcccc catagcgtga
     4081 caatttatcc cccatagtgt gacaatttat cccccatagt gtgacaattt atcccccact
     4141 acctatgtca atacttactc tcccataagg ttgctaatcc ctaacagtta ttattaacag
     4201 tatatctaac agtatatcta acagtatatc ttaagagcac atttaatttc ccgcctaaaa
     4261 aactcctact tttacctcca aaaaggttcg cgaatttttc taaactagaa aacttgctcc
     4321 ctacgggcgc taaaaaggtg tgatcgctac gctctcacat tgtacctgcg cagtcgcctc
     4381 cggctccttt gctaaaaccg ttgttcaaag aggaggtggg agaatctgag agcttgtcta
     4441 agccgttcta agacaattct tgattaactc ccttaatcta gtcgagaagc gccttacagg
     4501 cggtcagggc aagccctaac aacccgaaaa taaggtatac ttaaaatcgc cacacagatt
     4561 taattttgta gggtataata acttgtcgcc tatccgacag tcttgcgact ggaaggaggt
     4621 gtatctaatg tttgaacagt tcatttgccc ccttcttgta ggagtttgtg tctctttatt
     4681 cagttattgg ttgaataacc gaaacaagaa gtaaaatcag taaaggcgac atcaacctga
     4741 aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgatcatt
     4801 tgcacattca gtttaacatg agtttcaatc aaattcaatt ttgctgttgc tgatctaacc
     4861 attccttgac actttttaga tagtttaagg cggtttcttt ttttgtcgga ttttgcatgt
     4921 tagttgcttg ttgaaacagg ttttcgtagc gttcatatgt gacagattgt tgtttttctt
     4981 ttttttcttc ttcactggca aaccaagggt ttgtgtctcc aaatagatca ctacaggctt
     5041 cttctatctt tgtatctttt acatattgca cgtctttttt ggttcgctca tagttgtctt
     5101 gaacctcttt ttgctgtttt tctggcaagt tttgataaag acgagtgagt ttttccatgt
     5161 ttttatccgt caaacgtttt tcttgattca caaaaatatc gttcatttcc ttataacaag
     5221 tggtaaactc ttccattttt tgtgctaatg cttgctcttt tttcttttta tcacgcgctg
     5281 attcatactt ttgagtgaca tttcctaata aaatacttct agaaacgcgc ttatttccct
     5341 ctaaaacgcc gaataagccg cttgaaacga ttatcaagga aaatatacag ccaagaaaga
     5401 aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata tcttgtttga aaaattttat
     5461 agatgagcca aatacttcct gaaatactaa caaaaatgag tagatagggt aaataaaatg
     5521 ccattttagc ctccccatac taaccaaaat gcgaacagtg tcaggactaa gcaacataag
     5581 ggggcaagaa aatccaatga tcgttgaata agcacctttt tctgttcctt atttcgctgg
     5641 tctaatagct cttgatggag ttttttaact tgtaaatcaa tcgtagtttg tatcctttct
     5701 tgcgtataag gaacgagaaa agcacgttgt ttctttattt cgttttctat gtcttttaac
     5761 gttttagagc gataattctt gttggtggtc tgttcctctt tcaacgtgtt gttcatggtt
     5821 tcttcgagcc ggtctaattt ccggttgttc aggcgtaaga gttcgttgtt ctcttcgtac
     5881 ttcttctgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg ttcgctgttg
     5941 gttataggtt cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt cgtagtcctt
     6001 tcctagtctg ttggatcgta cttttttctt tttgtggatc aattcgtaag tgaaattttt
     6061 cccgcgattg aagcaatgaa tccccaatgt tttgagggaa ttacaaaatt ggtcataggt
     6121 tctggttttt ttgtttttca ttgtttgatc gatcgcaatc cgaatttcgt ctttccaaac
     6181 aggttcattt ttttctttta attttatttc agacatggtt cgtttttctg gtttctttcc
     6241 tcgctcagtg attggtaagt tatgtgcaag acaaatctcg tcatttaggt cataacattt
     6301 gtttagaaat gcttgaccat gagcttgaaa tttttttcca gtctccagat taacggagtt
     6361 aatgacaatg tgattatgaa tatggtcttt gtctgtatgt gtgtaaatag ctatttggtg
     6421 atcgggggct attttttttg catactctgc ccctagttga ttagcttttt cagcggtaat
     6481 ttctcctggt cgaaaagatt gaatcaataa gtgtgcctgt acacggtcgt cttttccata
     6541 cagcatgcga atttgtttca tttcactttt ggcataatcg acgtcacagt ttattcctgt
     6601 tttaatggtt gctcgtggtt cagcgtaatt aattgcacga ctacaagaag tggttcggct
     6661 tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag gttttcggat
     6721 aagcgttggg cgtctccagg agcaacgtcg tgttggtgtg tattacacca cttagcaatt
     6781 tgattgatat tccctccagc tttggctaaa tcgcgcgcga ttgcctgggc aatgtctggc
     6841 gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa agccggcaca
     6901 ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcggattc gctcactcga
     6961 aaactgattt gttttggctc ggatcgatag gtatattttt tagaatgatc gctagccaaa
     7021 ttttgatgtt gttcgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     7081 gcaataccat tgtacctaaa aaggtacaat gagtaaagct cactaaaagg gtttggcaag
     7141 ctctatattt gctagccaaa tacct
//
