LOCUS       NZ_CP066595             8512 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 107_1 plasmid p107-1_8kb,
            complete sequence.
ACCESSION   NZ_CP066595
VERSION     NZ_CP066595.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038094
            Assembly: GCF_016415325.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 158.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:50:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 43 of 116
            Pseudo Genes (incomplete)         :: 66 of 116
            Pseudo Genes (internal stop)      :: 30 of 116
            Pseudo Genes (multiple problems)  :: 19 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066595.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 107_1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p107-1_8kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            join(8496..8512,1..346)
                     /locus_tag="JCQ02_RS15385"
                     /old_locus_tag="JCQ02_15385"
                     /pseudo=""
     CDS             join(8496..8512,1..346)
                     /locus_tag="JCQ02_RS15385"
                     /old_locus_tag="JCQ02_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            567..1973
                     /locus_tag="JCQ02_RS15390"
                     /old_locus_tag="JCQ02_15390"
     CDS             567..1973
                     /locus_tag="JCQ02_RS15390"
                     /old_locus_tag="JCQ02_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002304901.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREENEREK
                     ALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            2562..3551
                     /locus_tag="JCQ02_RS15395"
                     /old_locus_tag="JCQ02_15395"
     CDS             2562..3551
                     /locus_tag="JCQ02_RS15395"
                     /old_locus_tag="JCQ02_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_002304903.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGSY
                     LEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIFMT
                     LTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPHFHL
                     ILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQEKAV
                     QEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRYKKKDE
                     NEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
                     /name="protein rep"
     gene            3548..3967
                     /locus_tag="JCQ02_RS15400"
                     /old_locus_tag="JCQ02_15400"
     CDS             3548..3967
                     /locus_tag="JCQ02_RS15400"
                     /old_locus_tag="JCQ02_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305964.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGIIL
                     LDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            3976..4284
                     /locus_tag="JCQ02_RS15405"
                     /old_locus_tag="JCQ02_15405"
     CDS             3976..4284
                     /locus_tag="JCQ02_RS15405"
                     /old_locus_tag="JCQ02_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002304909.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM"
                     /name="hypothetical protein"
     gene            4288..4650
                     /locus_tag="JCQ02_RS15410"
                     /old_locus_tag="JCQ02_15410"
     CDS             4288..4650
                     /locus_tag="JCQ02_RS15410"
                     /old_locus_tag="JCQ02_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002313294.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRKSQ
                     MSESGFASMFSDVNSR"
                     /name="hypothetical protein"
     gene            4873..6280
                     /locus_tag="JCQ02_RS15415"
                     /old_locus_tag="JCQ02_15415"
                     /pseudo=""
     CDS             4873..6280
                     /locus_tag="JCQ02_RS15415"
                     /old_locus_tag="JCQ02_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            6772..7760
                     /locus_tag="JCQ02_RS15420"
                     /old_locus_tag="JCQ02_15420"
                     /pseudo=""
     CDS             6772..7760
                     /locus_tag="JCQ02_RS15420"
                     /old_locus_tag="JCQ02_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            7757..8128
                     /locus_tag="JCQ02_RS15425"
                     /old_locus_tag="JCQ02_15425"
     CDS             7757..8128
                     /locus_tag="JCQ02_RS15425"
                     /old_locus_tag="JCQ02_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_233148746.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGIIL
                     LDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKTISCY"
                     /name="hypothetical protein"
     gene            8184..8492
                     /locus_tag="JCQ02_RS15430"
                     /old_locus_tag="JCQ02_15430"
     CDS             8184..8492
                     /locus_tag="JCQ02_RS15430"
                     /old_locus_tag="JCQ02_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002304909.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM"
                     /name="hypothetical protein"
     Typing          568..1970
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.359"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4874..5832
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.125"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agatattaaa gtgcaagaag aaaaattaaa aagaacaggc gaaacttcgc caacagaaga
       61 aacgattgat tgagaaagaa aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt
      121 ttgaatcttt tgctgatttt gagcggtggc tatcgtcgat ttctgacgat cagcaacacg
      181 aaaaatcagt gggtaatgag ttgcgagctg atgaaaaatc agcaccaagc gaacggaatt
      241 accctaacga atctgacgga atattcggag ggataccgaa tagaaaaagt cagatgagtg
      301 agagagtgga tttgcgtcaa tgtttagtga cgtaaattca cgataagcga atattttgca
      361 tgaagcaaaa taaagcgtaa aaggagttga gagcgtgaca cgctttcggc tcctttttcg
      421 ttgtcacttg tgacggcgaa aaccctcgga gagcgtttgg aattgtgaca gtcatgtcac
      481 aattaggaaa acgttactct ttgacaaaga gtaacagaaa ggtttacact ctttttatta
      541 gcgacagcgc gaaggagggg tttaacttgg ggtttagtat ttcgttcaaa aagggaacaa
      601 agaatacaag catttctcat aataatcgtg agttaactga tagccaaaaa atcaatgact
      661 ggcataagca tattgatttc acaaaatcgg atgaaaatat ctatttggaa caaataaata
      721 ttcgtgaaaa atatgaagaa ttattcggtg aagccgtaga aaaatataat gcaaagcaga
      781 aaagagccga tagaaaaatt gaggactatt tagcaaaggt tcgcaaggat aaaaaaatgg
      841 aaccacagcg agaatttatc gtgcaaattg gtactcttga tgattttcgt actactcgtg
      901 atgatggttc ttctactggc atatcggaac aacaagcaga acaaaatcga gtgattgcca
      961 acaaaatttt agttgagtat ttcaaagaat ttaaagaacg gaatcctagt ttatccgtct
     1021 acaatgctgt tattcataat gacgaagtaa gtccgcacct gcatttgaat attgttcctg
     1081 ttgctgaagg gtataaacgg ggtgttcaaa agcaaccaag ttttaacaaa gccttgcaac
     1141 agcagggttt acgagcagat aaagagaatg gtcgagtgat ttggaataat tttaggaatc
     1201 aggaagttga gtcaattgaa cgtttgatgg cagattttgg ttgggagcgt gagttagtcg
     1261 gtactaacaa aatcaaagat attcatgaat acaaagaaat catgagcgaa atttccgaat
     1321 tgcgtcaaac ggccgtaaaa gagcgtgaga gcgtttctga tgagttgagg tatatttgta
     1381 ggcaaagaga agaaaacgag cgtgagaagg ctctggtggc ttctgagaga gcgaagattg
     1441 aagaagagaa gaaaaatatg ctgaatgtaa aaaaagagat ggcggtcatt ttccaaaaag
     1501 atatggttcc tttagaaagt ttcaagaggc ttcctgatgg ttcgtatcat ttatctggga
     1561 atgattttgt tgatttgtat caacgagcta cgagagcaac aaatgccaag agaaatgctg
     1621 ataacatgag tgagcaatat ttacagatgc ttgagcaaaa tacagaatta aatgagtatg
     1681 tagctgcatt ggaagaagag cgtcatttgt ctggtagaaa acaattagca gatttacaag
     1741 tggaaaataa aaagctgaaa gaagaaaatc gctctttacg ccagcgttta gagcgattaa
     1801 aaaacgcatt tcaaaaatcg atcactcgtt tgtctatccg ttttgggctt gcagaaaagg
     1861 atatggggct ttctgaggaa ttagaagtat tagagcagga gaaaacctta ttttatcgtc
     1921 aaaacgtctc acagcataaa ttagagcgtt ctgaagatga gatcgagtgg tagttacgat
     1981 gtgcagtcaa cggtaccgga ggcggtttgt gccgaggata aggagttgac ggctaaacca
     2041 ctagcctatt aagctgacat ttgctaaaaa atggcaagtc cattttttgg ccaacttagc
     2101 gttagcgaag tttgggggcg ccctttttaa agggtgcggt tttatgtttg catgactaag
     2161 aatgagtggg aaaatttaac agccctatct aacaggcggt taaatgtaca aaatatgtac
     2221 acccccttaa aaatgcagtg gtcgattgat cgaattttat gtctttgggt tttggggttt
     2281 taaaacgaaa aaaagggggc gattttcgcc ctcttttttg atgttttttc ttatcttgat
     2341 actataggca actatttctg cgaagaaatc ggggctatag tatgggtcta tcccttgtgg
     2401 ctctaaggaa gaacaaaaaa aaagattgct ttttcttccc tagtatttta tagttttaat
     2461 tgactagaaa aaaactaaac tagaaaggag agcaatctat tttttgttat cggcttttca
     2521 gccttttttg tatacaaaat ttgtagattc agtatagcaa gatgaaaaag aaaaagcaag
     2581 atttgattaa cggaacattt cgagaaaaaa agaaaaattt gaaaaaagtg gtggcattta
     2641 gtgataatcg aatttctccc aaaatgcgtg aattgatgac aggctgtggt tcatatttag
     2701 aatttattgc aacgattgat aaggagaaaa agaaattggt ccaagctcac ttttgtaaaa
     2761 atcgtttctg tccgttatgt gcttggcgta aagctcgtaa ggattcgatg atgttgtcga
     2821 ttatgatgca agctattgcg gaagaaaagc agtatcaatt tatttttatg acgttgacga
     2881 caccgaatgt aaaagcggaa tgtttgaacg atgaaattga tttgtttaat catgcattga
     2941 aaaagatgtt tatgcgcaaa cgggttaaga cttcgattaa aggctatgtg cgaaaattgg
     3001 aaatcacgta caacaaaaaa cggaatgact ataacccgca ttttcacttg attttggcag
     3061 taaataaatc ttatttcacg gataaagatt attatatcaa gcaatcggaa tggttagatt
     3121 tgtggcggga agtgaccgga aaaacgggaa ttaatccgga tggtaccgac gaaattaccc
     3181 agttggatat tcgcaaggtc aaagggttcc aacaagaaaa agcagtacag gaagttgcga
     3241 aatattcggc aaaagatttt gaactgaccg aaagtcagga agtgtttgac acattctatc
     3301 atgcaatgaa aggtcgtcag ttgattacgt ttaacggtgt attcaaagaa tacaagaaaa
     3361 aatatgagtc gggtgacctt gatcgttata agaaaaaaga tgagaatgaa tatttttggt
     3421 ttttgactgc ttcgtggatg aggaaagaat cgaaatatgc ggttgattat cgtgagatga
     3481 gtgctgatga aagaaattat ttttcaggaa tgtatgtcga tgaaaaatct gttgaggggg
     3541 aaaatgaatg actgattcaa cggataaaat gacaattaag gcgcttgctg atgaactagg
     3601 aataaccaaa aatacagtga gaaattatct agtcaaagcc ggatatgatc ttgctcggta
     3661 caagaaaaat ggaattatct tgctagacaa agagttgatt ggtgttgctc ggtcaattta
     3721 tcggtcaaaa ggtggtcaat tgactggtca tttaccggtc aatagcacgg gcagtgattt
     3781 tcctttggaa aaagagaatg agtttttgcg tgatcaatta gctgagaagg acaaacatat
     3841 ttctgaatta acagaaatga ttcgtgatca acaaaaattg accctgcttc agcaacaaaa
     3901 aaacgatcag ttgttactag aaaatcaaga attaaaaaat cgaaaatggt ggcaattctg
     3961 gaagtgagat gtgacatgga taaaattgat ttgagagaag aaacagtcaa tagaattgaa
     4021 gtgatgtatg aacggttagc tgattatttg aatctttcgg atgaagataa ttatagtgag
     4081 atgcttgaac gaatgatgga tgttctcaac tcggttatca atgctgatct agcaaaatct
     4141 tcagttttga ctaatgaatt tgttcataag catttagcgc aaacaactga tgaagctgaa
     4201 aaagaatttt ggttgaaatt aatgagtcat attatgcagt taaaccaaat ttccgttatt
     4261 aattccaaag aaaaggggat gtagtttatg ggaatgactt tagcagatat taaagtgcaa
     4321 gaagaaaaat taaaaaaaga acaggcgaaa cttcgccaac agaagaaacg attgattgag
     4381 aaagaaaatg ctgcaatggg caaggctctt cgcaagcgtt ttgggtttga atcttttgct
     4441 gattttgagc ggtggctatc gtcgatttct gacgatcagc aacacgaaaa atcagtgggt
     4501 aatgagttgc gagctgatga aaaatcagca ccaagcgaac ggaattaccc taacgaatct
     4561 gacggaatat tcggagggat accgaataga aaaagtcaga tgagtgagag tggatttgcg
     4621 tcaatgttta gtgacgtaaa ttcacgataa gcgaatattt tgcatgaagc aaaataaagc
     4681 gtaaaaggag ttgagagcgt gacacgcttt cggctccttt ttcgttgtca cttgtgacgg
     4741 cgaaaaccct cggagagcgc ttggaattgt gacagtcatg tcacaattag gaaaaaacgt
     4801 tactctttga caaagagtaa cagaaaggtt tacactcttt ttattagcga cagcgcgaag
     4861 gaggggttta acttggggtt tagtatttcg ttcaaaaagg gaacaaagaa tacaagcatt
     4921 tctcataata atcgtgagtt aactgatagc caaaaaatca atgactggca taagcatatt
     4981 gatttcacaa aatcggatga aaatatctat ttggaacaaa taaatattcg tgaaaaatat
     5041 gaagaattat tcggtgaagc cgtagaaaaa tataatgcaa agcagaaaag agccgataga
     5101 aaaattgagg actatttagc aaaggttcgc aaggataaaa aaatggaacc acagcgagaa
     5161 tttatcgtgc aaattggtac tcttgatgat tttcgtacta ctcgtgatga tggttcttct
     5221 actggcatat cggaacaaca agcagaacaa aatcgagtga ttgccaacaa aattttagtt
     5281 gagtatttca aagaatttaa agaacggaat cctagtttat ccgtctacaa tgctgttatt
     5341 cataatgacg aagtaagtcc gcacctgcat ttgaatattg ttcctgttgc tgaagggtat
     5401 aaacggggtg ttcaaaagca accaagtttt aacaaagcct tgcaacagca gggtttacga
     5461 gcagataaag agaatggtcg agtgatttgg aataatttta ggaatcagga agttgagtca
     5521 attgaacgtt tgatggcaga ttttggttgg gagcgtgagt tagtcggtac taacaaaatc
     5581 aaagatattc atgaatacaa agaaatcatg agcgaaattt ccgaattgcg tcaaacggcc
     5641 gtaaaagagc gtgagagcgt ttctgatgag ttgaggtata tttgtaggca aagagaagaa
     5701 aacgagcgtg agaaggctct ggtggcttct gagagagcga agattgaaga agagaagaaa
     5761 aaatatgctg aatgtaaaaa aagagatggc ggtcattttc caaaaagata tggttccttt
     5821 agaaagtttc aagaggcttc ctgatggttc gtatcattta tctgggaatg attttgttga
     5881 tttgtatcaa cgagctacga gagcaacaaa tgccaagaga aatgctgata acatgagtga
     5941 gcaatattta cagatgcttg agcaaaatac agaattaaat gagtatgtag ctgcattgga
     6001 agaagagcgt catttgtctg gtagaaaaca attagcagat ttacaagtgg aaaataaaaa
     6061 gctgaaagaa gaaaatcgct ctttacgcca gcgtttagag cgattaaaaa acgcatttca
     6121 aaaatcgatc actcgtttgt ctatccgttt tgggcttgca gaaaaggata tggggctttc
     6181 tgaggaatta gaagtattag agcaggagaa aaccttattt tatcgtcaaa acgtctcaca
     6241 gcataaatta gagcgttctg aagatgagat cgagtggtag ttacgatgtg cagtcaacgg
     6301 taccggaggc ggtttgtgcc gaggataagg agttgacggc taaaccacta gcctattaag
     6361 ctgacatttg ctaaaaatgg caagtccatt ttttggccaa cttagcgtta gctaaatgta
     6421 caaaatatgt acaccccctt aaaaatgcag tggtcgattg atcgaatttt atgtctttgg
     6481 gttttggggt tttaaaacga aaaaaggggg cgattttcgc cctctttttg atgtttttct
     6541 tatcttgata ctataggcaa ctatttctgc gaagaaatcg gggctatagt atgggtctat
     6601 cccttgtggc tctaaggaag aacaaaaaaa aaaaagattg ctttttcttc cctagtattt
     6661 tatagtttta attgactaga aaaaactaaa ctagaaagga gagcaatcta ttttttgtta
     6721 tcggcttttc agcctttttg tatacaaaat ttgtagattc agtatagcaa gatgaaaaag
     6781 aaaaagcaag atttgattaa cggaacattt cgagaaaaaa agaaaaattt gaaaaaagtg
     6841 gtggcattta gtgataatcg aatttctccc aaaatgcgtg aattgatgac aggctgtggt
     6901 tcatatttag aatttattgc aacgattgat aaggagaaaa agaaattggt ccaagctcac
     6961 ttttgtaaaa atcgtttctg tccgttatgt gcttggcgta aagctcgtaa ggattcgatg
     7021 atgttgtcga ttatgatgca agctattgcg gaagaaaagc agtatcaatt tatttttatg
     7081 acgttgacga caccgaatgt aaaagcggaa tgtttgaacg atgaaattga tttgtttaat
     7141 catgcattga aaaagatgtt tatgcgcaaa cgggttaaga cttcgattaa aggctatgtg
     7201 cgaaaattgg aaatcacgta caacaaaaaa cggaatgact ataacccgca ttttcacttg
     7261 attttggcag taaataaatc ttatttcacg gataaagatt attatatcaa gcatcggaat
     7321 ggttagattt gtggcgggaa gtgaccggaa aaacgggaat taatccggat ggtaccgacg
     7381 aaattaccca gttggatatt cgcaaggtca aagggttcca acaagaaaaa gcagtacagg
     7441 aagttgcgaa atattcggca aaagattttg aactgaccga aagtcaggaa gtgtttgaca
     7501 cattctatca tgcaatgaaa ggtcgtcagt tgattacgtt taacggtgta ttcaaagaat
     7561 acaagaaaaa atatgagtcg ggtgaccttg atcgttataa gaaaaaagat gagaatgaat
     7621 atttttggtt tttgactgct tcgtggatga ggaaagaatc gaaatatgcg gttgattatc
     7681 gtgagatgag tgctgatgaa agaaattatt tttcaggaat gtatgtcgat gaaaaatctg
     7741 ttgaggggga aaatgaatga ctgattcaac ggataaaatg acaattaagg cgcttgctga
     7801 tgaactagga ataaccaaaa atacagtgag aaattatcta gtcaaagccg gatatgatct
     7861 tgctcggtac aagaaaaatg gaattatctt gctagacaaa gagttgattg gtgttgctcg
     7921 gtcaatttat cggtcaaaag gtggtcaatt gactggtcat ttaccggtca atagcacggg
     7981 cagtgatttt cctttggaaa aagagaatga gtttttgcgt gatcaattag ctgagaagga
     8041 caaacatatt tctgaattaa cagaaatgat tcgtgatcaa caaaaattga ccctgcttca
     8101 gcaacaaaaa acgatcagtt gttactagaa aatcaagaat taaaaaatcg aaaatggtgg
     8161 caattctgga agtgagatgt gacatggata aaattgattt gagagaagaa acagtcaata
     8221 gaattgaagt gatgtatgaa cggttagctg attatttgaa tctttcggat gaagataatt
     8281 atagtgagat gcttgaacga atgatggatg ttctcaactc ggttatcaat gctgatctag
     8341 caaaatcttc agttttgact aatgaatttg ttcataagca tttagcgcaa acaactgatg
     8401 aagctgaaaa agaattttgg ttgaaattaa tgagtcatat tatgcagtta aaccaaattt
     8461 ccgttattaa ttccaaagaa aaggggatgt agtttatggg aatgacttta gc
//
