LOCUS       NZ_CP066588             7165 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 103 plasmid p103_7kb, complete
            sequence.
ACCESSION   NZ_CP066588
VERSION     NZ_CP066588.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038093
            Assembly: GCF_016415105.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:58:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,056
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 101 of 175
            Pseudo Genes (incomplete)         :: 73 of 175
            Pseudo Genes (internal stop)      :: 35 of 175
            Pseudo Genes (multiple problems)  :: 30 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066588.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 103"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p103_7kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            join(6509..7165,1..81)
                     /locus_tag="JCP83_RS15490"
                     /old_locus_tag="JCP83_15490"
     CDS             join(6509..7165,1..81)
                     /locus_tag="JCP83_RS15490"
                     /old_locus_tag="JCP83_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            110..631
                     /locus_tag="JCP83_RS15495"
                     /old_locus_tag="JCP83_15495"
     CDS             110..631
                     /locus_tag="JCP83_RS15495"
                     /old_locus_tag="JCP83_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_031592680.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENNNAI
                     TTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            715..1167
                     /locus_tag="JCP83_RS15500"
                     /old_locus_tag="JCP83_15500"
     CDS             715..1167
                     /locus_tag="JCP83_RS15500"
                     /old_locus_tag="JCP83_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025089292.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKVMLKNHTKTLILLFHFVIFLSLVFTLIQSKFFYFSHS
                     IATWLLIFFLSLLLVINTLIIIFNKHKNLFLHSVFPIILLSFLYFV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1578..2732
                     /locus_tag="JCP83_RS15505"
                     /old_locus_tag="JCP83_15505"
     CDS             1578..2732
                     /locus_tag="JCP83_RS15505"
                     /old_locus_tag="JCP83_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_044383358.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            2705..3148
                     /locus_tag="JCP83_RS15510"
                     /old_locus_tag="JCP83_15510"
     CDS             2705..3148
                     /locus_tag="JCP83_RS15510"
                     /old_locus_tag="JCP83_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            3452..3832
                     /locus_tag="JCP83_RS15515"
                     /old_locus_tag="JCP83_15515"
     CDS             3452..3832
                     /locus_tag="JCP83_RS15515"
                     /old_locus_tag="JCP83_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3814..4728
                     /locus_tag="JCP83_RS15520"
                     /old_locus_tag="JCP83_15520"
     CDS             3814..4728
                     /locus_tag="JCP83_RS15520"
                     /old_locus_tag="JCP83_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4688..4966
                     /locus_tag="JCP83_RS16010"
     CDS             4688..4966
                     /locus_tag="JCP83_RS16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            4968..5657
                     /locus_tag="JCP83_RS15525"
                     /old_locus_tag="JCP83_15525"
     CDS             4968..5657
                     /locus_tag="JCP83_RS15525"
                     /old_locus_tag="JCP83_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031592678.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     KEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(5778..5864)
                     /locus_tag="JCP83_RS15530"
                     /old_locus_tag="JCP83_15530"
     CDS             complement(5778..5864)
                     /locus_tag="JCP83_RS15530"
                     /old_locus_tag="JCP83_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_109746925.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          6510..7165
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3815..4725
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggaaataaga taggatctct gcaaatttat tttgaagaaa atataccttc aattacctta
       61 gataattggt tagagaaata ataacctaac aatttaggag gcaattatca tgcaacccaa
      121 agaaaccttt acaataaagg aattatctga attatttgaa ataagtaggc aagcaatttc
      181 taaacatgtt gataaattag acccgtcctt attagctaaa aatgagcgtg gttataaggt
      241 tgtattacgt tctggagtat tgcaacttgc tagcaactta ggtaaccaaa ggttactaga
      301 taggttgcat gagtatgaac aagaaaacga taaaaacgtt tatatatcaa gaaaaaacgt
      361 ggttgtaaac gagttgctag ataagttgcg tgaagaaaat gaatttttac gagaacaact
      421 ttctctcaaa gagcaaaata attctaatat gcaaaaatta ctcgaccagc aacaacaact
      481 caccttgcag gcaaatcaac agatcgaaaa actgcaagaa cagttacaat tagcttatac
      541 ggaagaacct gaagaagagg tattaacttc tgtcaataaa gaaaacaaca atgcaataac
      601 aacagaaaaa aagtggtgga aattttggta acaattacca actttccata aagaagaaat
      661 caatttctat agactttatc tttctaaaaa taaaaaatat gtttaggtga tcaaatgact
      721 ataagtaaag taatcaaaca aaaaagaata gaaaaacaac taactcaaga agatttagct
      781 gaaatgcttt tagtatctaa aaaaaccatt tctaattggg aaaatggtag aacaatacca
      841 gatacagaaa atttaattca atttgctaag ttatttgatt tatcactaga caatattttg
      901 ttaggagaag aaaaagttat gttaaaaaat catacaaaaa cactaatact attatttcat
      961 tttgtaattt ttttaagttt agtttttacc ttaattcagt ccaaattctt ttattttagc
     1021 cattcaattg ccacttggtt attaatattt tttctaagtt tgctattagt aatcaatact
     1081 ctgattatta tttttaataa acataaaaat ttatttcttc atagtgtttt tccaattatt
     1141 ttgctgagtt ttttatactt tgtataaagt ataaaaaaag actgctcttt cgagcagcca
     1201 gttaaaaaaa taatgagagt ttattcttga aaaagcaacg cgcttataaa gcgctaccaa
     1261 aattttatca tattttataa aaaataacaa tccagtaaaa aatgactggt aaaaattcac
     1321 aaagaaacgg aaagaagcaa ctgcagaaaa tcaagcctta aaaaaagctt ttgacctaga
     1381 ccaatgaaaa atcacaaagt gagcagctat agcccttgat agaggggcta cttggcatag
     1441 tcgacattat gttaactaac gctataaata acgattatgt tcccaaaaca atattttatg
     1501 aactataaca aaaatttgtt atcgcatatt gtatatttta attataggtg tataccactt
     1561 aaatttaaca caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc
     1621 tcttttcctt gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa
     1681 attctaattt caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa
     1741 ctgattttga acactccata acttcaattc ttttaatgga aaataacacc gaaactgata
     1801 ttaactatac tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt
     1861 ctattttctt tccagtattt tcagacgctg ataatacgat taaatatatt tataccgtca
     1921 aaaaaaatac aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc
     1981 aaaatctagt agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt
     2041 attattccga taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa
     2101 atggtgtttc ttcctttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa
     2161 ttaaaaattt ctctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct
     2221 ccggagataa ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt
     2281 ggtgtgctgc ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt
     2341 cagctagagc cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa
     2401 aaagtcttaa tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa
     2461 aagtagcacg aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca
     2521 tttatatggg aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat
     2581 ttgggtgggt caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact
     2641 acccaactgt aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt
     2701 ttacttggga caataccatt acaaattggt aaataaaccc attgctttag ttatgggtct
     2761 agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct
     2821 gattcctttt ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa
     2881 tttaaatact tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa
     2941 aattcaaatc attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac
     3001 aaataattta ctaacactgc gctctaataa aaaacaatta gattacactt ttagtttgga
     3061 ttctccaaaa aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa
     3121 accaattgaa ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat
     3181 ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt
     3241 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaaa
     3301 agcggttatt tgctagccaa atacgtaggt atttggctag caaatataga gcttgccaaa
     3361 cccttttagt gagctttact cattgtacct ttttaggtac aatggtattg cgaacgatac
     3421 gtccgtagga cgtactgaaa gggtgggact tatgagcgaa caaaatcaaa atttggctag
     3481 cgatcattct aaaaaatata cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag
     3541 cgaatctgaa tttgcaaagc tgaagcagtc agctgaagct ttgcaaatga gtgtgccggc
     3601 tttcgtaaaa gccaaggcac aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat
     3661 tgcccaggca atcgcgcgcg atttagccaa agctggaggg aatatcaatc aaattgctaa
     3721 gtggtgtaat acacaccaac acgacgttgc tcctggagac gcccaacgct tatccgaaaa
     3781 cctaaaaatc atgcaaaagg agctacaaaa gatatggcaa cagttaaagt aagccgaacc
     3841 acttcttgta gtcgtgcaat taattacgct gaaccacgag caaccattaa aacaggaata
     3901 aactgtgacg tcgattatgc caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa
     3961 gacgaccgtg tacaggcaca cttattgatt caatcttttc gaccaggaga aattaccgct
     4021 gaaaaagcta atcaactagg ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata
     4081 gctatttaca cacatacaga caaagaccat attcataatc acattgtcat taactccgtt
     4141 aatctggaga ctggaaaaaa atttcaagct catggtcaag catttctaaa caaatgttat
     4201 gacctaaatg acgagatttg tcttgcacat aacttaccaa tcactgagcg aggaaagaaa
     4261 ccagaaaaac gaaccatgtc tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa
     4321 gacgaaattc ggattgcgat cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac
     4381 caattttgta attccctcaa aacattgggg attcattgct tcaatcgcgg gaaaaatttc
     4441 acttacgaat tgatccacaa aaagaaaaaa gtacgatcca acagactagg aaaggactac
     4501 gaaaaggaga cgattttaca tgagctggac agacgagaac gaacctataa ccaacagcga
     4561 accaacgagc tacgaagagc tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga
     4621 gaacaacgaa ctcttacgcc tgaacaaccg gaaattagac cggctcgaag aaaccatgaa
     4681 caacacgttg aaagaggaac agaccaccaa caagaattat cgctctaaaa cgttaaaaga
     4741 catagaaaac gaaataaaga aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat
     4801 acaaactacg attgatttac aagttaaaaa actccatcaa gagctattag accagcgaaa
     4861 taaggaacag aaaaaggtgc ttattcaacg atcattggat tttcttgccc ccttatgttg
     4921 cttagtcctg acactgttcg cattttggtt agtatgggga ggctaaaatg gcattttatt
     4981 taccctatct actcattttt gttagtattt caggaagtat ttggctcatc tataaaattt
     5041 ttcaaacaag atattcatta aaaggctcta aaattcgatt taagcgattt ttctttcttg
     5101 gctgtatatt ttccttgata atcgtttcaa gcggcttatt cggcgtttta gagggaaata
     5161 agcgcgtttc tagaagtatt ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg
     5221 ataaaaagaa aaaagagcaa gcattagcac aaaaaatgga agagtttacc acttgttata
     5281 aggaaatgaa cgatattttt gtgaatcaag aaaaacgttt gacggataaa aacatggaaa
     5341 aactcactcg tctttatcaa aacttgccag aaaaacagca aaaagaggtt caagacaact
     5401 atgagcgaac caaaaaagac gtgcaatatg taaaagatac aaagatagaa gaagcctgta
     5461 gtgatctatt tggagacaca aacccttggt ttgccagtga agaagaaaaa aaagaaaaac
     5521 aacaatctgt cacatatgaa cgctacgaaa acctgtttca acaagcaact aacatgcaaa
     5581 atccgacaaa aaaagaaacc gccttaaact atctaaaaag tgtcaaggaa tggttagatc
     5641 agcaacagca aaattgaatt tgattgaaac tcatgttaaa ctgaatgtgc aaatgatcaa
     5701 acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt ttcaggttga
     5761 tgtcgccttt actgatttta cttcttgttt cggttattca accaataact gaataaagag
     5821 acacaaactc ctacaagaag ggggcaaatg aactgttcaa acattagata cacctccttc
     5881 cagtcgcaag actgtcggat aggcgacaag ttattatacc ctacaaaatt aaatctgtgt
     5941 ggcgatttta agtatacctt attttcgggt tgttagggct tgccctgacc gcctgtaagg
     6001 cgcttctcga ctagattaag ggagttaatc aagaattgtc ttagaacggc ttagacaagc
     6061 tctcagattc tcccacctcc tctttgaaca acggttttag caaaggagcc ggaggcgact
     6121 gcgcaggtac aatgtgagag cgtagcgatc acaccttttt agcgcccgta gggagcaagt
     6181 tttctagttt agaaaaattc gcgaaccttt ttggaggtaa aagtaggagt tttttaggcg
     6241 ggaaattaaa tgtgctctta agatatactg ttagatatac tgttagatat actgttaata
     6301 ataactgtta gggattagca accttatggg agagtaagta ttgacatagg tagtggggga
     6361 taaattgtca cactatgggg gataaattgt cacactatgg gggataaatt gtcacgctat
     6421 gggggataaa tcgtcacact atgggggata aattgtattt tacaccctct tatgtctatg
     6481 ataacataat ttattgaggt gatttttatt gagtaacgat atagttcgat ataataatgg
     6541 attaaattct gtccctttaa gaaaatttac ccctatagat atggatttat tttggggaat
     6601 atgttctaaa atgaaaagaa aaggtacaaa agaagaaatt tttacattta aacaaataaa
     6661 agaattaatt gattacaata taaatgcacg tggcgaccgt tttgctaatg atttaataaa
     6721 aatgtcaaaa aaaatcatta ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca
     6781 attaaatctg tttcaaaaat ttaaaatcga taaagtgaat gaaacattat taataaaggt
     6841 ttctgaagaa tttgaatata tacttaattc aatcggaact aattttactc gtttcgaatt
     6901 agaaaatatg acaaacttaa agagtagcta cgttaaagag ttatatcgcc aacttatggc
     6961 tcataaagac agaagatcta aaaaaggtca ctggtttgta aaaatagaag attttagagc
     7021 tctattagca ataccagaaa gttatcgcat gagagatatt gataaacaaa tttttgccca
     7081 agcgaaaaaa gaatttttaa acgttaattc aactcaccct gctattttta aaacttttga
     7141 aatcaaaaaa attaaagcct acaaa
//
