LOCUS       NZ_CP066581             8059 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 100_1 plasmid p100_1_8kb,
            complete sequence.
ACCESSION   NZ_CP066581
VERSION     NZ_CP066581.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038091
            Assembly: GCF_016415285.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,141
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 51 of 141
            Pseudo Genes (incomplete)         :: 84 of 141
            Pseudo Genes (internal stop)      :: 34 of 141
            Pseudo Genes (multiple problems)  :: 23 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066581.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 100_1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p100_1_8kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            1..945
                     /locus_tag="JCP75_RS15985"
                     /old_locus_tag="JCP75_15985"
     CDS             1..945
                     /locus_tag="JCP75_RS15985"
                     /old_locus_tag="JCP75_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..1499
                     /locus_tag="JCP75_RS15990"
                     /old_locus_tag="JCP75_15990"
     CDS             966..1499
                     /locus_tag="JCP75_RS15990"
                     /old_locus_tag="JCP75_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1514..1684
                     /locus_tag="JCP75_RS15995"
                     /old_locus_tag="JCP75_15995"
     CDS             1514..1684
                     /locus_tag="JCP75_RS15995"
                     /old_locus_tag="JCP75_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165353901.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2309..3457)
                     /gene="tnpB"
                     /locus_tag="JCP75_RS16000"
                     /old_locus_tag="JCP75_16000"
     CDS             complement(2309..3457)
                     /gene="tnpB"
                     /locus_tag="JCP75_RS16000"
                     /old_locus_tag="JCP75_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(3474..3878)
                     /gene="tnpA"
                     /locus_tag="JCP75_RS16005"
                     /old_locus_tag="JCP75_16005"
     CDS             complement(3474..3878)
                     /gene="tnpA"
                     /locus_tag="JCP75_RS16005"
                     /old_locus_tag="JCP75_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            4016..4249
                     /locus_tag="JCP75_RS16010"
                     /old_locus_tag="JCP75_16010"
     CDS             4016..4249
                     /locus_tag="JCP75_RS16010"
                     /old_locus_tag="JCP75_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_198777526.1"
                     /translation="MGDFCLILLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNK
                     EKCWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            4269..4556
                     /locus_tag="JCP75_RS16015"
                     /old_locus_tag="JCP75_16015"
     CDS             4269..4556
                     /locus_tag="JCP75_RS16015"
                     /old_locus_tag="JCP75_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            5006..5386
                     /locus_tag="JCP75_RS16020"
                     /old_locus_tag="JCP75_16020"
     CDS             5006..5386
                     /locus_tag="JCP75_RS16020"
                     /old_locus_tag="JCP75_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5368..6282
                     /locus_tag="JCP75_RS16025"
                     /old_locus_tag="JCP75_16025"
     CDS             5368..6282
                     /locus_tag="JCP75_RS16025"
                     /old_locus_tag="JCP75_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6527..7216
                     /locus_tag="JCP75_RS16030"
                     /old_locus_tag="JCP75_16030"
     CDS             6527..7216
                     /locus_tag="JCP75_RS16030"
                     /old_locus_tag="JCP75_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1..8059
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5369..6279
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctcgc aacttagaca
     1141 atcaaaggtt gctagataag ttgcataaac atgaacaaaa caacgataaa aatgcttata
     1201 taccaaacaa aaacgtggtt gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaacttgcc ctcaaagagc aaaataattc tcatatacaa aaactactcg
     1321 accagcaaca acaactcacc ctgcaagcca accaacaaat cgaaaaactt caggagcaac
     1381 tgcagttgac ttatacggaa gagtcttcag agaatagttc aacgactctt tctgaagcaa
     1441 cagaaacaat ggacgaacaa acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa
     1501 gaaggaaata cttatgacaa aaaaacactt gctattcggt tctatcctac taggaatatt
     1561 aagctttagc ccaatgttat tatcagtggc caacaaagga aataaaaaag gggaactaaa
     1621 actcaatcct aattactaca acagtcctaa cgaagaattg tttgtaaaag aaacggataa
     1681 ataataaaaa agactgctct tccgagcagc cagttaaaaa ataatgagtg tttattcttg
     1741 aaaaagcaac gcgcttatga agcgctacca aaaatttatc atattttatg aaaaataaca
     1801 ccccagtaaa aaatgactgg taaaaattca caaagaaacg gaaagaagca actgcagaaa
     1861 atcaagcctt aaaaagagct tttgacctag accaattaaa aatcacaaag tgagcgtctc
     1921 tatccattgg tagaggggct acttggcata gtgtacatta tgtctactag ctgtttaaat
     1981 aactgatctt ataattttat ttaaacgatt atgaattcaa aactactttt tatgacgtta
     2041 atcattgtta tataataaaa tgttatataa taaaattact aagattattt aatataacaa
     2101 gcaattttaa aaaaacgaaa aggaaaaaca atcatgaaaa agaaagtagt gaactactca
     2161 ccacttagct aaacctgacg gttcttaacg cttgaagtgg gagcttcttg ggaatagagc
     2221 atacttgata gcgtatttct ttactaacag cggtgtctct tatttaccaa gctatccccg
     2281 tagttcctac ggttcttgat gattggatct aagccaatcc catagaatgt agtcttagac
     2341 cttcggtcag aatattgata ctagcattga tatcacgatc atgatgagta tgacaaacag
     2401 gacaagtcca ttctcgaatt tcaagagatt tcttgccatc tttatgtccg cactctgaac
     2461 aaatctgact agacggaaac catttatcta ctttgatgat ttttcgtcca taccagtcag
     2521 ccttgtattg taatttagtc acaaactttg accaagaaac atcagaaata cttttagcta
     2581 atttatgatt tcgcaacata ccttttgtgt ttaagtcttc aatacagata atatcgtgat
     2641 ttttgatcat ttctgtactc aacttattca gaaaatcagt acgttgattc attacttttt
     2701 catgcaatct agctactttg cgtttttgtt tttgataatt tttagcttca aatagattgg
     2761 tacctttatt tttagccaat aaagcacgcc tagacaattt gcgttgttca cgctttagtt
     2821 tcttttccat tttggacgtg aatttattat tatcaatttt ttgtccatca gaaagaatcg
     2881 caaagtccat aatacctaag tcaataccaa ttgtggaatt agttttaggt aattcgataa
     2941 tttcttcttt acacaacaaa gaaatatagt atttaccgct agaatgacgt gatattgtag
     3001 cagatttgat aatccctttt ggttgtctat gaagcttaat tcttactaat gacttcaatt
     3061 taggaacttt gatgaattta ctatcaatca aagcaactgt accattttga ttattcgttg
     3121 tataactctg aacaggattt ttcttacttt tgaaacgtgg aaatccaacg gatttatccc
     3181 gaaaaaaatt cttgtatgct ttatctaaat taagttgggc attggctaaa gcaaggctat
     3241 caatttcttt caaaaacgga aattctttct tgtatttagc tggtgtcgga aaagtcattt
     3301 ttttagaaga atcattttta acttcttcat acgccttttt tcggtcatca agcattagat
     3361 tgtagacctt acggacacaa ccaaaagatt tggcaaagaa gatttcttgt tcctctgttg
     3421 gatagattct gaatttgtat gcttttagtt gttccataaa gttcacctac tttctattta
     3481 tgattgtttc cttgattttg gatatacttc ttaatcacat caattggtgc gccaccagtt
     3541 gtcagaagac aaaaactttt agaccagaac atttctttcc aaagaaattg tttgactctt
     3601 ggaaagtcac gttttatcaa tcttgagctg gcacttttat aggcattgat gaattttgtc
     3661 aattctgttt taggttgagc tttgaacata atatgaacgt ggtctttatc atgattccac
     3721 tccactaaag taatgtgata cgattctgaa attctttcaa aagtagtttt ggcataatca
     3781 gatatttcat catcaatcac ttgtctacga tatttcgtta ctaaaacaag gtggtaataa
     3841 agaagaaata ctgaatgatt atttgtatct aattccatgt tatatcaact catttcttgt
     3901 atagactgat tgtaacatgg aacaaaataa aaaggcaata cgccttagtt gtcggtcttc
     3961 gagttaccaa cggtaaccct cataccgaac ggcattcatc acccacctat agaggatggg
     4021 cgacttctgc ctaattctgt taaaacattg tgttattcta ggaatattag gaacttgtct
     4081 agctggcatc ggtacaggaa taaaagttga tgcagctact tactatggaa atggtcttta
     4141 ttgtaacaaa gaaaaatgtt gggtagattg gaatcaagct aaaggagaaa ttggaaaaat
     4201 tattgttaat ggttgggtta atcatggtcc atgggcacct agaaggtagt ggtagggagg
     4261 ttattataat ggattttact aaagaagaaa aacttttaaa tgcaattagt aaagtataca
     4321 atgaagcaac tatagatgac tatcctgact taaaagaaaa gctctttctt tattctaaag
     4381 aaatcagtga gggaaaaagt gttggtgaag ttagtatgaa attaagtagt tttcttggaa
     4441 gatatatttt aaaacataaa tttggattac ctaaatcttt aatagaatta caagaaattg
     4501 ttagtaagga atctcaagta tatagaggat gggcttctat tggtatttgg agttaattct
     4561 ttggaataaa atgaaaatgg gactgaagaa tcaattctga ttcatcagtc ccataaatta
     4621 tagagccgaa aaattatcat atttatagat ttattaatag taaatggcat aagaatcttt
     4681 gaacaacgaa aaaaatgtag ctctacctaa ttattgattt gcaacaattt caccgaaggt
     4741 gaaacaagcg aaactttaca aaaagtggct agcatttgct agtctttttt gtattgtgga
     4801 gagtgggggg gagggggatt caagggggag cttgcgcccc tttgacaagc ggttatttgc
     4861 accaaatacg taggtatttg gctagcaaat atagagcttg ccaagccctt tagtgagctt
     4921 tagtgagctt tacttattgt acctttttag gtacaatggt attgcgaacg atacgtccgt
     4981 aggacgtact gaaagggtgg gacttatgag cgaacaaaat caatatttgg ctagcaatca
     5041 ttctaaaaaa tatacctatc gatccgagcc aaaacaaatc agttttcgag tgagcgaatc
     5101 cgaatttgca aagctgaagc agtcagctga agctttgcaa atgagtgtgc cggctttcgt
     5161 gaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgctca
     5221 ggcaatcgcg cgcgatttag ccaaagctgg cgggaatatc aatcaaattg ccaagtggtg
     5281 caacacacac caacacgacg ttgatcctgg cgacgcccaa cgcttatccg aaaacctaaa
     5341 aatcatgcaa aaggagctac aaaagatatg gcaacagtta aagtaagccg aaccacttct
     5401 tgtagccgag cgatcaatta cgccgaacca cgagcaaccg tcaaaacagg gataaactgt
     5461 gacattgatt atgccaaaag cgaaatgaaa caaattcgca tgctgtatgg aaaagacgat
     5521 cgtgtacaag cacacttatt gatccagtct tttcgaccag gagaaattac cgctgaaaaa
     5581 gccaatcaac taggggcaga gtatgcgaaa aaaatagccc ctgaccacca aatcgcgatt
     5641 tacacacata cagacaaaga ccatattcat aaccacattg tcattaactc cgtcaatctg
     5701 gaaactggga acaaatttca agcccatggt caagcatttt taaacaaatg ttatgaccta
     5761 aatgacgaga tttgtcttga acatgactta acaatcactg aacgagggaa gaaacccgaa
     5821 aaacgaacca tgtctgaaat caaattaaaa gaaaaaaatg aacctgtttg gaaagacgaa
     5881 attcggtttg cgattgatca aacaatgaaa aacaaaaaaa ctcgaacgta taaccaattt
     5941 tgtgattctc ttaaaacatt cggcattcat tgcttgaatc gtgggaaaaa ttttacttac
     6001 gaattgatcc acaaaaagaa aaaagtacga tccaacaaac taggaaagga ttacgaaaag
     6061 gagacgattt tacatgagct ggacagacga gaacgagcct acaatcaaca acgaaccaac
     6121 gagctacgaa gagctcgagc tagcattcgc gaaactgcaa aagaagtacg aagagaacaa
     6181 cgaactctta cgactgaaca accggaaatt agaccgactc gaagaaacca tgaccaacac
     6241 gttgaaagag gagcagacta ccaacaagaa ttatcgttct aaaacgttac aagacataga
     6301 aaatgaaata aagaaacaac gtgcttttct tgcaccttat accaaagaaa gaatacaaac
     6361 tacaattgat tcacaagtca aaaaattcca ccatgcgcta ttaaaccagc gaaataaaga
     6421 acagaaaaaa gtaattatgc agcgatcaat ggatattctt gcccccttat gttgcttaat
     6481 catgacactg ttagcctttt ggctggtatg gggagaataa atagaaatgg cattctattt
     6541 accctatcta ctcatttttg ccagtatttt aggaattatt tggcttagct ataaggctta
     6601 tcaaacaaga tattcattaa aaggctctaa aattcgattt aagaggtttt tattgccagg
     6661 ggttatattt acattatcaa tcgttttaag tggtttattc ggcgttttag agggaaataa
     6721 acgtatttca agaagtattt tattagggga tatcactcaa aagtatgaag cagcatgtga
     6781 taaaaagaaa aaggagcaag tgttaacaca aaaaatggaa gagtttacca cttgttatga
     6841 ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa
     6901 actcactcgt ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta
     6961 tgagcgaacc aaaaaagacg tgcaatattt taaagatacg cagacagaag aagcttgtta
     7021 taatttattc agtgatacaa tccctttttc aactagtgaa caggaacgaa aagaacggca
     7081 acaatcagtt acctacgaac gttataaagc actgcttcag caagcaacga acatacaaaa
     7141 tccaacgaaa aaagaaacgg cattaaatta tctaaaaagt gtcaaggaat ggttagatca
     7201 gcaacagcaa aattgaattt gatcggcaat catgttaaac taaacatgca aatgatcaaa
     7261 cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt tcaggttgat
     7321 gtcgccttta ttgattttac ttcttgtttc ggttattcaa ccaataactg aataaagaga
     7381 cacaaacttc tataagaagg gggcaaatga acaattcaaa cattagatac acctccttcc
     7441 agtcgcaaga ctgtcggata ggcgacaagt tattatatct taaaaaatta aacttgtgtg
     7501 gtgtttttaa gtatcgctta ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     7561 gctttaaagc tagataagga tttttatcca agactgtctt aaaacggctt agataagcat
     7621 tcagaaccgc tcaaaggcct ctttaccaac atgtttttag caaaggagcc tttaggcgac
     7681 tgcgcagtac aatgtgagag cgtagcgatc acaccttttt ggcgaccgta ggaagcaagg
     7741 ttttggattg gcagttttga aaaatgcgcg aacctgttta aagaaaaaag aagtggtttt
     7801 taggcgggaa aattagcccc cctcttttta tatctctttt taaacctctt tttaaacctc
     7861 tttaccgtaa gctgtgtcct tactctacca atggatacag aatgttatga aggacatttt
     7921 gtctgtttat gaaggacatt ttgtctgttt atgaaggaca ttttgtctgt ttatgaagga
     7981 cattttgtct gtttgaacgt caaatatgaa tgtcttgaca tttaaaaaat gtcattcata
     8041 attggattga ggtgattat
//
