LOCUS       NZ_CP066580            11477 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 100_1 plasmid p100_1_11kb,
            complete sequence.
ACCESSION   NZ_CP066580
VERSION     NZ_CP066580.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038091
            Assembly: GCF_016415285.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,141
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 51 of 141
            Pseudo Genes (incomplete)         :: 84 of 141
            Pseudo Genes (internal stop)      :: 34 of 141
            Pseudo Genes (multiple problems)  :: 23 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066580.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 100_1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p100_1_11kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(219..581)
                     /locus_tag="JCP75_RS15905"
                     /old_locus_tag="JCP75_15905"
     CDS             complement(219..581)
                     /locus_tag="JCP75_RS15905"
                     /old_locus_tag="JCP75_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(585..893)
                     /locus_tag="JCP75_RS15910"
                     /old_locus_tag="JCP75_15910"
     CDS             complement(585..893)
                     /locus_tag="JCP75_RS15910"
                     /old_locus_tag="JCP75_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(1044..1316)
                     /locus_tag="JCP75_RS15915"
                     /old_locus_tag="JCP75_15915"
     CDS             complement(1044..1316)
                     /locus_tag="JCP75_RS15915"
                     /old_locus_tag="JCP75_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_233152379.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFCVNN"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1309..1542)
                     /locus_tag="JCP75_RS15920"
                     /old_locus_tag="JCP75_15920"
     CDS             complement(1309..1542)
                     /locus_tag="JCP75_RS15920"
                     /old_locus_tag="JCP75_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(1545..2473)
                     /locus_tag="JCP75_RS15925"
                     /old_locus_tag="JCP75_15925"
                     /pseudo=""
     CDS             complement(1545..2473)
                     /locus_tag="JCP75_RS15925"
                     /old_locus_tag="JCP75_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349395.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(2870..>3728)
                     /locus_tag="JCP75_RS15930"
                     /old_locus_tag="JCP75_15930"
                     /pseudo=""
     CDS             complement(2870..>3728)
                     /locus_tag="JCP75_RS15930"
                     /old_locus_tag="JCP75_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            complement(3703..4050)
                     /locus_tag="JCP75_RS15935"
                     /old_locus_tag="JCP75_15935"
     CDS             complement(3703..4050)
                     /locus_tag="JCP75_RS15935"
                     /old_locus_tag="JCP75_15935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_233152381.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPGHISELTEMIRDQQNLTLLQQQKKRSVVTRKSRI
                     KNRNDCQQNFS"
                     /name="hypothetical protein"
     gene            complement(4043..4276)
                     /locus_tag="JCP75_RS15940"
                     /old_locus_tag="JCP75_15940"
     CDS             complement(4043..4276)
                     /locus_tag="JCP75_RS15940"
                     /old_locus_tag="JCP75_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(4279..5245)
                     /locus_tag="JCP75_RS15945"
                     /old_locus_tag="JCP75_15945"
                     /pseudo=""
     CDS             complement(4279..5245)
                     /locus_tag="JCP75_RS15945"
                     /old_locus_tag="JCP75_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(5694..7099)
                     /locus_tag="JCP75_RS15950"
                     /old_locus_tag="JCP75_15950"
                     /pseudo=""
     CDS             complement(5694..7099)
                     /locus_tag="JCP75_RS15950"
                     /old_locus_tag="JCP75_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            complement(7320..7682)
                     /locus_tag="JCP75_RS15955"
                     /old_locus_tag="JCP75_15955"
     CDS             complement(7320..7682)
                     /locus_tag="JCP75_RS15955"
                     /old_locus_tag="JCP75_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(7686..7994)
                     /locus_tag="JCP75_RS15960"
                     /old_locus_tag="JCP75_15960"
     CDS             complement(7686..7994)
                     /locus_tag="JCP75_RS15960"
                     /old_locus_tag="JCP75_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(8003..8422)
                     /locus_tag="JCP75_RS15965"
                     /old_locus_tag="JCP75_15965"
     CDS             complement(8003..8422)
                     /locus_tag="JCP75_RS15965"
                     /old_locus_tag="JCP75_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(8415..8648)
                     /locus_tag="JCP75_RS15970"
                     /old_locus_tag="JCP75_15970"
     CDS             complement(8415..8648)
                     /locus_tag="JCP75_RS15970"
                     /old_locus_tag="JCP75_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(8651..9619)
                     /locus_tag="JCP75_RS15975"
                     /old_locus_tag="JCP75_15975"
     CDS             complement(8651..9619)
                     /locus_tag="JCP75_RS15975"
                     /old_locus_tag="JCP75_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002298422.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(10068..11474)
                     /locus_tag="JCP75_RS15980"
                     /old_locus_tag="JCP75_15980"
     CDS             complement(10068..11474)
                     /locus_tag="JCP75_RS15980"
                     /old_locus_tag="JCP75_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002333830.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENEREK
                     ALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     Typing          complement(1541..2427)
                     /name="rep_cluster_935"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.492"
                     /coverage="87.0"
                     /evalue="0.0"
     Typing          complement(4287..5160)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.918"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(8659..9536)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.973"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(3229..3728)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.808"
                     /coverage="100.0"
                     /evalue="7.129999999999999e-98"
     Typing          complement(5698..6176)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="6.709999999999996e-106"
     Typing          complement(6390..7099)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.312"
                     /coverage="100.0"
                     /evalue="1.36e-152"
     Typing          complement(10072..11474)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.65"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaccctcc ttcaggctgt cgctaataaa aagagtgtaa acctttctgt tactctttgt
       61 caaagagtaa cattttccta attgtgacat ggctgtcaca attccaaatg ctctccgagg
      121 gtattcgctg tcacaagtga caacgaaaaa aggagccgaa agcgtgtcac gctctcaact
      181 ccttttacgc tttattttgc ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta
      241 aatattgacg taaatttact ctcactcatc tgacttttct tattcggtat cactccgaat
      301 aagccgtcag attcatcggg ataatctcgt tcgcttggcg ctgatttttc atcagcaagc
      361 cactcattat ccactgattt ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc
      421 tcaaaatcag caaaagtttc aaacccaaaa cgcttccgaa gagccttgcc cattgcagca
      481 ttttctttct caatcaagcg ttttttctgt tggcgaagtt tcgcttgttc tttttttaat
      541 ttttcttctt gcgctttaat atctgctaaa gtcattccca taaactacat ctccttttct
      601 ttggaattaa taacggaaat ttggtttaac tgcataacat gactcattaa tttcaaccaa
      661 aattcttttt ctgattcatc agttgtttgc gctaaatgct tatgaacaaa ttcattagtc
      721 aaaactgaag attttgctag atcagcattg ataaccgagt tgagaacatc catcattcgt
      781 tcaagcattt cactataatt atcttcatcc ggcaaattca aataatcagc taaccgttca
      841 tacatcaatt caattctatc gactgtttct tctctcaaat caattttatc catgtcacac
      901 ctcacttcca gaattgccac cattttcgat ttttaattct tgattttcta gtaacaactg
      961 atcgtttttg ttgctgaagc aaagtcaatt ttgttggtca cgaatcattt ccgttaattc
     1021 agaaatatgc ctatcttttt ccgctaattg ttcacgcaaa attcattttc tttttctaac
     1081 gggaaatcat tgcctgcatc attgaccggt aaatcaccag tcaattgacc accttttgac
     1141 cgataaattg accgcacaac accaatcaat tccttgtcta acatgataac cccattatct
     1201 ttataccgag caaggtcata acctgcattg accagttgat tgcgcactgt atttttagat
     1261 atacccaact catcagcaag gttttttatg gtcatttttt ctggcaaatc agtcattatc
     1321 ttctccgtta ctttcagcta ccccataaac atatttaaca gcatgcttag ggataatgat
     1381 tggggaatca attttattat ttttcaaaat caactcatac tgcgttacat caagtaactt
     1441 accggacatt tttttgctat ccaaaaaaac aacgacaacg ttatcacctg ttaaattcac
     1501 caaccaattt ttcttaccaa aatgcttttg tttttctgcc attcttattc ctcctccgtt
     1561 acatctaacc cttgaagcca cgtatccaat tcttttttta attcgtctgc agattcctta
     1621 aacgtctctc gtaaatgatt cgttaactca tcattttgac tttctctttt caaaatatct
     1681 ctcaatttgt atttcattct acgctctaaa aacccccaca tttcagggtt agcaatcagt
     1741 ccaaaaatca tcaccttatc aataggtttt atatcaatcg gcaaaaaatg cgggcttgca
     1801 aaactatcta agctttcctg aaccatcgca tgccaatccg ttgctttgcc acgtcttagc
     1861 tgcatttcta accgccacca cgtatcgatt tcttttggca cgattacttt ttttcgttcc
     1921 tgctctagct ttttgttgta cagtcgcact tggcgctcac tggcacgact gccccagtac
     1981 gctgtttcta gcttgccaga acggccgtag attggcttaa aagaaatcgg gtctacaata
     2041 cgatactgag taataaagtc atccggcaca tctacaatat cgcaggcaat atccgcccgg
     2101 gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt tcatggaact agctaaaaac
     2161 tggcctatct tattcggatt aaaatcaata cgaccttttc cttcatgaaa tttaagtatt
     2221 tcgatatacg caatatcttc tccatattga tcgaataatt gccagccgtt tatcccaact
     2281 gatttcaaat atccctgatt ttcgttcaaa cgcattaatt gctcgaaatc gacaaaaaca
     2341 actccatttt tcgtccaata agctatggta tcattcagtt ttccgacaat tgtaattcga
     2401 tcaatcgacc aatttaattt tagggggtgg gtgtacataa ccgcctgttt gtacatttaa
     2461 ccgcctgtta gatagggctg ttcttttttt cccactcatt tacgattatg ccactttact
     2521 caaattttgc cattttcgtc ctccaaacga aaaaagagac ttttggaaag aatttccaga
     2581 gttcacgtaa ccctaaaaat tcatatccaa aaatctctag cttgaagaaa cctaaaaata
     2641 ttcgtataat gaacctgagt tacggagcta ggtgaagtag tttccgtaaa tagtcgagag
     2701 tgttagcgca ctgctcggct tttattttgc tcttttttca gtttatttat ttgataactc
     2761 aataatagca cttctcttta gctatgcaaa taaaaaaaaa accgcgggaa tttgaaaggg
     2821 cgccccaaac ttcgctaacc taggttggcc aaaaatggac ttgccatttt tagcaaatgt
     2881 cagcttcagg gattagtggt ttagccgtca actccttatc ctcggcacaa accgcctccg
     2941 gtaccgttga ctgcacatcg taacaccact cgatctcatt tcagaacgct ctaatttacg
     3001 ctgtgagacg ttttgacgat aaaataaggt tttctcctgc tcgactaact aaacaagacc
     3061 aaaaataaac gagtgatcga tttttgaaaa accgctcaga acgcaaaata tgccatttta
     3121 gagctattgt agaaaatgta gtttgaaatg caattttctt ctttcagctt tttatttccc
     3181 acttgtaaat ctgctaattg ctttagctcg ttgatacaaa tcaacaactt ctcttcttca
     3241 atcttcactc tctcagaagc caccagagcc ttttctcgct cattttcttc tctttgccta
     3301 gaaatatacc tcaactcatc agaaacgctc tcacgctctt ttacggccgt ttgacgcaat
     3361 tctgaaatct cgctcatgat ttctttatat tcgtgaacat ctttgatttt gttagtaccg
     3421 actaactcac gctcccaacc aaaatctgcc atcaaacgtt caattgactc aacttcctga
     3481 ttcctaaaat tattccaaat cactcgacca ttctctttat ctgctcgtaa accctgctgt
     3541 tgcaaggctt tgttaaaact tggttgcttt tgaacaccct gtttataccc ttcagcaaca
     3601 ggaacaatat tcaaatgcag gtgcggactt acttcgtcat tatgaataac agcattgtag
     3661 acggataaat taggattccg ttctttaaat tctttgaaat attcaactaa aattttgttg
     3721 gcaatcattt cgatttttaa ttcttgattt tctagtaaca actgatcgtt ttttttgttg
     3781 ctgaagcaaa gtcaaatttt gttggtcacg aatcatttcc gttaattcag aaatatgccc
     3841 cggtaaatca ccagtcaatt gaccaccttt tgaccgataa attgaccgca caacaccaat
     3901 caattccttg tctaacatga taaccccatt atctttatac cgagcaaggt cataacctgc
     3961 attgaccagt tgattgcgca ctgtattttt agatataccc aactcatcag caaggttttt
     4021 tatggtcatt ttttctggca aatcagtcat tatcttctcc gttactttca gctaccccat
     4081 aaacatattt aacagcatgc ttagggataa tgattgggga atcaatttta ttatttttca
     4141 aaatcaactc atactgcgtt acatcaagta acttaccgga catttttttg ctatccaaaa
     4201 aaacaacgac aacgttatca cctgttaaat tcaccaacca atttttctta ccaaaatgct
     4261 tttgtttttc tgccattctt attcctcctc cgttacatct aacccttgaa gccaagtgtc
     4321 taattctttt ttcaaaacat ctgctgattc cgaaaatgtt tctcgtaaat gattcgtcaa
     4381 ctcatcattt tgactttctc ttttcaaaat atctctcaat ttgtatttca ttctacgctc
     4441 taaaaacccc cacatttcag ggttagcaat cagtccaaaa atcatcacct tatcatttgt
     4501 ttttacatca agtggtagaa aatgagggct agcaaaacta tttaaacttt catgaaccat
     4561 tgcatgccaa tctgttgctt tcccacgcct taactgcaat tccagtcgcc accaagtttc
     4621 tatttctggt ggcacaattt tacgcttggt ttcctgttcc aattttttgt tgtatagccg
     4681 aatctggcgt tcgctggcac gactgcccca ataagctgtt tccagcttcc cagaccgccc
     4741 ataaattggc ttgaaagaaa caggatcaac cactcgatat tgtgtaataa actcatcagg
     4801 cacatcgata atatcgcaag caatatccgc acgtgagaaa tgtggttcta gaaataaatc
     4861 atgaatgaaa tttttcattg aactagctaa aaatgaccaa tcttattcgg gttaaaatca
     4921 atccgccctt tgccatcttg ccatttcaaa atctcaatat aagcaatatt ttcttcgtat
     4981 tggtctaata attgccagcc gttattccca accgctttta aatagccatt gccttcattc
     5041 acccgcatta attgctcaaa atctaaaatc aacacatcat tttgagtgtg ataataaata
     5101 ttttccttca gttttccaac gatagtaatt cggtcaattg accactgcat ttttaagggg
     5161 tgtacatatt ttgtacattt aaccgcctgt tagatagggc tgttaaattt tcccactcat
     5221 ttacgattat gccattttta ctcaaatttt gttcctcctc aaagaaaaag agacttctgg
     5281 aaagaatttt caaaagttca cgtaactctt aaaaattcat taccaaaaaa tctctagtct
     5341 tgaagaaatc taaaaatatt cgtataatga ttaagaagtt acggagctag tgcgattagt
     5401 ttccgtaaag gtcgagaacg tgtccagcgt tgctcgactt tatttttttg ctcttttttc
     5461 agtttattta tttgataact caataatagc acttctcttt agctatgcaa atatccaaaa
     5521 aataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta ggttggccaa
     5581 aaaatggact tgccattttt tagcaaatgt cagcttcagg gattagtggt ttagccgtca
     5641 actccttatc ctcggcacaa accgcctccg gtaccgttga ctgcacatcg taactaccac
     5701 tcgatctcat cttcagaacg ctctaattta cgctgtgaga cgttttgacg ataaaataag
     5761 gttttctcct gctctaacac ttctaattcc tcagaaagcc ccatatcctt ttctgcaaga
     5821 ccaaaacgga tagacaaacg agtgatcgat ttttgaaatg cgttttttaa tcgctctaaa
     5881 cgctggcgta aagagcgatt ttcttctttc agctttttat tttccacttg taaatctgct
     5941 aattgttttc taccagacaa atgacgctct tcttccaatg tagctacata ctcatttaat
     6001 tctgtatttt gctcaagcat ctgtaaatat tgctcactca tgttatcagc atttctcttg
     6061 gcatttgttg ctctcgtagc tcgttgatac aaatcaacaa aatcattccc agataaatga
     6121 tacgaaccat caggaagcct cttgaaactt tctaaaggaa caatatcttt tggaaaatga
     6181 ccgccatctc ttttttacat tcagcatatt tttcttctct tcttcaatct tcactctctc
     6241 agaagccacc agagcctttt ctcgctcatt ttcttctctt tgcctagaaa tatacctcaa
     6301 ctcatcagaa acgctctcac gctcttttac ggccgtttga cgcaattctg aaatctcgct
     6361 catgatttct ttatattcgt gaacatcttt tgattttgtt agtaccgact aactcacgct
     6421 cccaaccaaa atctgccatc aaacgttcaa ttgactcaac ttcctgattc ctaaaattat
     6481 tccaaatcac tcgaccattc tctttatctg ctcgtaaacc ctgctgttgc aaggctttgt
     6541 taaaacttgg ttgcttttga acaccccgtt tatacccttc agcaacagga acaatattca
     6601 aatgcaggtg cggacttact tcgtcattat gaataacagc attgtagacg gataaattag
     6661 gattccgttc tttaaattct ttgaaatatt caactaaaat tttgttggca atcactcgat
     6721 tttgttctgc ttgttgttcc gatatgccag tagaagaacc atcatcacga gtagtacgaa
     6781 aatcatcaag agtaccaatt tgcacgataa attctcgctg tggttccaat tttttatcct
     6841 tgcgaacctt tgctaaatag tcctcgattt ttctatcggc tcttttctgc tttgcattat
     6901 atttttctac ggcttcaccg aataattctt catatttttc acgaatattt gtttgttcta
     6961 aatagatatt ttcatccgat tttgtgaaat caatatgctt atgccagtca ttgatttttt
     7021 ggctatcagt taactcacga ttattatgag aaatgcttgt attctttgtt ccctttttga
     7081 acgaaatact gaaccccaag ttaaaaccct ccttcggctg tcgctaataa aaagagtgta
     7141 aacctttctg ttactctttg tcaaagagta acattttcct aattgtgaca tggctgtcac
     7201 aattccaaat gctctccgag ggtattcgct gtcacaagtg acaacgaaaa aaggagccga
     7261 aagcgtgtca cgctctcaac tccttttacg ctttattttg cttcacgcaa aataatcgct
     7321 tatcgtaaat tcacgtcatt aaatattgac gtaaatttac tctcactcat ctgacttttc
     7381 ttattcggta tcactccgaa taagccgtca gattcatcgg gataatctcg ttcgcttggc
     7441 gctgattttt catcagcaag ccactcatta tccactgatt tttcgtgttg ctgatcgtca
     7501 gaaatcgacg atagccaccg ctcaaaatca gcaaaagttt caaacccaaa acgcttccga
     7561 agagccttgc ccattgcagc attttctttc tcaatcaagc gttttttctg ttggcgaagt
     7621 ttcgcttgtt ctttttttaa tttttcttct tgcgctttaa tatctgctaa agtcattccc
     7681 ataaactaca tctccttttc tttggaatta ataacggaaa tttggtttaa ctgcataaca
     7741 tgactcatta atttcaacca aaattctttt tctgattcat cagttgtttg cgctaaatgc
     7801 ttatgaacaa attcattagt caaaactgaa gattttgcta gatcagcatt gataaccgag
     7861 ttgagaacat ccatcattcg ttcaagcatt tcactataat tatcttcatc cggcaaattc
     7921 aaataatcag ctaaccgttc atacatcaat tcaattctat cgactgtttc ttctctcaaa
     7981 tcaattttat ccatgtcaca cctcacttcc agaattgcca ccattttcga ttttttaatt
     8041 cttgattttc tagtaacaac tgatcgtttt tttgttgctg aagcaaagtc aatttttgtt
     8101 ggtcacgaat catttccgtt aattcagaaa tatgcctatc tttttccgct aattgttcac
     8161 gcaaaaattc attttctttt tctaacggga aatcattgcc tgcatcattg accggtaaat
     8221 caccagtcaa ttgaccacct tttgaccgat aaattgaccg cacaacacca atcaattcct
     8281 tgtctaacat gataacccca ttatctttat accgagcaag gtcataacct gcattgacca
     8341 gttgattgcg cactgtattt ttagatatac ccaactcatc agcaaggttt tttatggtca
     8401 ttttttctgg caaatcagtc attatcttct ccgttacttt cagctacccc ataaacatat
     8461 ttaacagcat gcttagggat aatgattggg gaatcaattt tattattttt caaaatcaac
     8521 tcatactgcg ttacatcaag taacttaccg gacatttttt tgctatccaa aaaaacaacg
     8581 acaacgttat cacctgttaa attcaccaac caatttttct taccaaaatg cttttgtttt
     8641 tctgccattc ttattcctcc tccgttacat ctaacccttg aagccaagtg tctaattctt
     8701 ttttcaaaac atctgctgat tccgaaaatg tttctcgtaa atgattcgtc aactcatcat
     8761 tttgactttc tcttttcaaa atatctctca atttgtattt cattctacgc tctaaaaacc
     8821 cccacatttc agggttagca atcagtccaa aaatcatcac cttatcattt gtttttacat
     8881 caagtggtag aaaatgaggg ctagcaaaac tatttaaact ttcatgaacc attgcatgcc
     8941 aatctgttgc tttcccacgc cttaactgca attccagtcg ccaccaagtt tctatttctg
     9001 gtggcacaat tttacgcttg gtttcctgtt ccaatttttt gttgtatagc cgaatctggc
     9061 gttcgctggc acgactgccc caataagctg tttccagctt cccagaccgc ccataaattg
     9121 gcttgaaaga aacaggatca accactcgat attgtgtaat aaactcatca ggcacatcga
     9181 taatatcgca agcaatatcc gcacgtgaga aatgtggttc tagaaataaa tcatgaatga
     9241 aatttttcat tgaactagct aaaaaatgac caatcttatt cgggttaaaa tcaatccgcc
     9301 ctttgccatc ttgccatttc aaaatctcaa tataagcaat attttcttcg tattggtcta
     9361 ataattgcca gccgttattc ccaaccgctt ttaaatagcc attgccttca ttcacccgca
     9421 ttaattgctc aaaatctaaa atcaacacat cattttgagt gtgataataa atattttcct
     9481 tcagttttcc aacgatagta attcggtcaa ttgaccactg catttttaag ggggtgtaca
     9541 tattttgtac atttaaccgc ctgttagata gggctgttaa attttcccac tcatttacga
     9601 ttatgccatt tttactcaaa ttttgttcct cctcaaagaa aaagagactt ctggaaagaa
     9661 ttttcaaaag ttcacgtaac tcttaaaaat tcattaccaa aaaatctcta gtcttgaaga
     9721 aatctaaaaa tattcgtata atgattaaga agttacggag ctagtgcgat tagtttccgt
     9781 aaaggtcgag aacgtgtcca gcgttgctcg actttatttt tttgctcttt tttcagttta
     9841 tttatttgat aactcaataa tagcacttct ctttagctat gcaaatatcc aaaaaataaa
     9901 accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg ccaaaaaatg
     9961 gacttgccat tttttagcaa atgtcagctt cagggattag tggtttagcc gtcaactcct
    10021 tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta ccactcgatc
    10081 tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa taaggttttc
    10141 tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc aagaccaaaa
    10201 cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc taaacgctgg
    10261 cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc tgctaattgt
    10321 tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt taattctgta
    10381 ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct cttggcattt
    10441 gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa atgatacgaa
    10501 ccatcaggaa gcctcttgaa actttctaaa ggaacaatat ctttttggaa aatgaccgcc
    10561 atctcttttt ttacattcag catatttttc ttctcttctt caatcttcac tctctcagaa
    10621 gccaccagag ccttttctcg ctcattttct tctctttgcc tagaaatata cctcaactca
    10681 tcagaaacgc tctcacgctc ttttacggcc gtttgacgca attctgaaat ctcgctcatg
    10741 atttctttat attcgtgaac atctttgatt ttgttagtac cgactaactc acgctcccaa
    10801 ccaaaatctg ccatcaaacg ttcaattgac tcaacttcct gattcctaaa attattccaa
    10861 atcactcgac cattctcttt atctgctcgt aaaccctgct gttgcaaggc tttgttaaaa
    10921 cttggttgct tttgaacacc ccgtttatac ccttcagcaa caggaacaat attcaaatgc
    10981 aggtgcggac ttacttcgtc attatgaata acagcattgt agacggataa attaggattc
    11041 cgttctttaa attctttgaa atattcaact aaaattttgt tggcaatcac tcgattttgt
    11101 tctgcttgtt gttccgatat gccagtagaa gaaccatcat cacgagtagt acgaaaatca
    11161 tcaagagtac caatttgcac gataaattct cgctgtggtt ccaatttttt atccttgcga
    11221 acctttgcta aatagtcctc gatttttcta tcggctcttt tctgctttgc attatatttt
    11281 tctacggctt caccgaataa ttcttcatat ttttcacgaa tatttgtttg ttctaaatag
    11341 atattttcat ccgattttgt gaaatcaata tgcttatgcc agtcattgat tttttggcta
    11401 tcagttaact cacgattatt atgagaaatg cttgtattct ttgttccctt tttgaacgaa
    11461 atactgaacc ccaagtt
//
