LOCUS       NZ_CP066537            10060 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WCHKP090374 plasmid p1_090374, complete
            sequence.
ACCESSION   NZ_CP066537
VERSION     NZ_CP066537.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15649164
            Assembly: GCF_014041505.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 090374
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Wei,L., Wen,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 02:50:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,845
            CDSs (total)                      :: 5,723
            Genes (coding)                    :: 5,557
            CDSs (with protein)               :: 5,557
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 58 of 166
            Pseudo Genes (incomplete)         :: 112 of 166
            Pseudo Genes (internal stop)      :: 17 of 166
            Pseudo Genes (multiple problems)  :: 21 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066537.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP090374"
                     /isolation_source="environmental surface"
                     /db_xref="taxon:573"
                     /plasmid="p1_090374"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-07-09"
     gene            complement(30..395)
                     /locus_tag="H3G96_RS29340"
     CDS             complement(30..395)
                     /locus_tag="H3G96_RS29340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(392..763)
                     /gene="tolA"
                     /locus_tag="H3G96_RS28910"
                     /old_locus_tag="H3G96_028915"
     CDS             complement(392..763)
                     /gene="tolA"
                     /locus_tag="H3G96_RS28910"
                     /old_locus_tag="H3G96_028915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(1037..1282)
                     /locus_tag="H3G96_RS28915"
                     /old_locus_tag="H3G96_028920"
     CDS             complement(1037..1282)
                     /locus_tag="H3G96_RS28915"
                     /old_locus_tag="H3G96_028920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1609..3294
                     /locus_tag="H3G96_RS28920"
                     /old_locus_tag="H3G96_028925"
     CDS             1609..3294
                     /locus_tag="H3G96_RS28920"
                     /old_locus_tag="H3G96_028925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3304..3561
                     /locus_tag="H3G96_RS28925"
                     /old_locus_tag="H3G96_028930"
     CDS             3304..3561
                     /locus_tag="H3G96_RS28925"
                     /old_locus_tag="H3G96_028930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3646..3795
                     /locus_tag="H3G96_RS28930"
                     /old_locus_tag="H3G96_028935"
     CDS             3646..3795
                     /locus_tag="H3G96_RS28930"
                     /old_locus_tag="H3G96_028935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5279..7243
                     /locus_tag="H3G96_RS28935"
                     /old_locus_tag="H3G96_028940"
     CDS             5279..7243
                     /locus_tag="H3G96_RS28935"
                     /old_locus_tag="H3G96_028940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7243..7974
                     /gene="mobC"
                     /locus_tag="H3G96_RS28940"
                     /old_locus_tag="H3G96_028945"
     CDS             7243..7974
                     /gene="mobC"
                     /locus_tag="H3G96_RS28940"
                     /old_locus_tag="H3G96_028945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7981..8511
                     /locus_tag="H3G96_RS28945"
                     /old_locus_tag="H3G96_028950"
     CDS             7981..8511
                     /locus_tag="H3G96_RS28945"
                     /old_locus_tag="H3G96_028950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8538..8717)
                     /locus_tag="H3G96_RS28950"
                     /old_locus_tag="H3G96_028955"
     CDS             complement(8538..8717)
                     /locus_tag="H3G96_RS28950"
                     /old_locus_tag="H3G96_028955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8743..9171)
                     /locus_tag="H3G96_RS28955"
                     /old_locus_tag="H3G96_028960"
     CDS             complement(8743..9171)
                     /locus_tag="H3G96_RS28955"
                     /old_locus_tag="H3G96_028960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(9335..9649)
                     /locus_tag="H3G96_RS28960"
                     /old_locus_tag="H3G96_028965"
     CDS             complement(9335..9649)
                     /locus_tag="H3G96_RS28960"
                     /old_locus_tag="H3G96_028965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9637..9915)
                     /locus_tag="H3G96_RS28965"
                     /old_locus_tag="H3G96_028970"
     CDS             complement(9637..9915)
                     /locus_tag="H3G96_RS28965"
                     /old_locus_tag="H3G96_028970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     BGC             1..8294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3899..4477)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7244..7971
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ctattgcagc ctgcgggtta aaggacctgt tactggcggc aaccatcatc attgagcttt
       61 tgggtggtga ctggtcgcgg atcctgtaag ccgtcatttt tcagactgaa ttcaaaattt
      121 ataacctgtc gcattgccgg gtgccctgcg ggatatagcc agtgcctcat tgccgtcagc
      181 gtttcccgaa aaaaaaggtg acagccggta agcgacacgt tgtccactcg tccatcgtcc
      241 agaatatcaa acgtggcggt cacctgacct tcgatgcgtt ttgctgcggc ctgtaccggg
      301 taggttggct gcagtgtggt tacgggtttt agtggtatcg catgtaccgg agtgaggaac
      361 atggccgcca gcactgtcag agtaaaagct ttcatggtct gaaatccaga ggggcgtttt
      421 tgaatatttg ccaggtattt tcatccggtg ccggcggaat tttggcgtgg gtaacggctg
      481 atattgctgc tttacagagt ttcggatctc ctccttcagc aatggcgctg ttcagagtgc
      541 catcacgttg tatggatatc cgcacggtgc acatttttcc cgcataggcc tctggttcaa
      601 acatctgcat ttcaacagcg gtcttgatgg ccgcagcgta gctactgatt ttagatgact
      661 tatccagagt ataaattttc ggcggctggg tggtggaatg cttacaggcc gtcaggagta
      721 aggggctgca caatgccagc aggataaagg gtattaaacg catcgggttg atgacctgtc
      781 agtaaaaaat gttacatgta tatttgtcac tctttacggc cacgttcatt tacataaaca
      841 cggcttatcg tatcgttttt acaccaaacg gcccgccatg cagttgcgtg cataggcatg
      901 cagcgggtta caatttaccg ggcgcgtttc cggcggtttt ccgggtggtt tgttgcctgt
      961 tttatcccgt agccgccgga aacgccctca gaccgtctga gcggtgcggg caatgcgacg
     1021 ttatggtaaa taccccttac cggcggcgct gtggctgcca ggtgaaggtg gtttccgtag
     1081 cctcgtattt ctcccgacgc atttcctgcg cccgcctggc ttcacgttcg tcctgaatgg
     1141 cacgaagcac cctttcccgc acctgttgtt ttaccgcctt tggcagcgtt ttgttccctt
     1201 tcaggcccat ggccagtgcc tgagcgacaa aggcgtccgc caggcgcttt tcttcagcgg
     1261 gagtcagttt caggtgcgtc atagtggcct ccgggcgtct caggtttccc gaacagttta
     1321 accgcaggtc ctctcctcat gccagttagc tttcagatgt gtctatacca tctgaaatgt
     1381 aagtgtgatg aatgtcttaa ataataaaat ttgttgccat gagaaatttt cgggaaaaga
     1441 cgcgatctgc ttacaggtgg ctccgggcgc ttttttgtgc ctgaagatga gaactaatct
     1501 catatattga cacctgaaaa cgggaggagt aaggtaataa ttatactgtg tatatgtaca
     1561 gtatgttggc tttaatttat tgttttaaat gtcaaagagg aaaacggtat gagtggtgga
     1621 gacggtcgag gtccgggtaa ttcaggtctg ggacataatg gtggtcaggc cagtgggaat
     1681 gtgaacggta cgtctggtaa aggtggccct tcatcaggtg gtggtacgga tccaaacagc
     1741 gggccgggct ggggtacgac gcatacgccg aatggagata ttcataacta taatccgggg
     1801 gagtttggta acggcgggag taaacccggt ggtaatggtg gtaacagcgg caatcatagc
     1861 ggtagctctg gtggcggaca gtcttcggcc actgcgatgg ccttcggtct gcctgccctg
     1921 gctactccgg gggctgaagg actggcttta tcagtttccg gcgatgcgtt gtcggccgcc
     1981 gttgctgatg tgctggctgc cctgaagggg ccgtttaagt ttggtctgtg ggggattgcg
     2041 atctacggtg tgctgccttc tgagattgca aaagatgatc cgaaaatgat gtcaaaaatt
     2101 atgacgtcat taccggccga tgcggtgacg gagactccgg caagtacttt accactggac
     2161 caggcgacgg ttcgtgtcag acaacgggtt gtggatgtgg tgaaggatga gcggcagcat
     2221 attgcggttg tcgcaggtcg gccaatgagt gtccctgtgg tggatgcgaa accgacaaaa
     2281 cgtccggggg tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt gagcgtaccg
     2341 aaaggtgttc cgacagcgaa agccccgcca aaaggcattg ttgctgaaaa aggtgattca
     2401 cgtccggctg gttttacagc cggtggtaac tcccgtgagg ccgttattcg tttcccgaaa
     2461 gagaccggac agaagccggt ttatgtgtcg gtgacagatg ttcttacccc ggcacaggta
     2521 aaacagcgtc aggaggaaga aaagcgtcgc cagcaggcat gggacgccgc tcatccggaa
     2581 gaggggctga aaagagacta tgataaagcg aaagctgagc tggatgccga agataaaaat
     2641 attgcgacct taaacagccg cattgcatcg acagagaagg cgctccccgg tgcaagggct
     2701 gctgttcagg aagccgataa aaaggtaaaa gaggcagagg caaataaaga tgattttgtg
     2761 acttataacc cgcctcatga atatggctcc gggtggcagg atcaggttcg ctatcttgat
     2821 aaggatattc agaatcagaa tgagaaatta aaagcggctc aggcatcttt aaacgcaatg
     2881 aatgaatcct tatccagaga taaggctgca cttaccgggg cgatggagag ccggaaacaa
     2941 aaggagaaaa aagcgaagga tgcagaaaat aagttaaatg aggaaaagaa caagcctcgc
     3001 aaggggacta aagactacgg ccatgattat catccagtac ctaaaacaga agacataaag
     3061 ggacttggag agttaaagcg aggtgatcct aaaacaccta aacaaggtgg tggtggtaag
     3121 cgggcccgat ggtatggcga taaaaaacgt aaaatttatg aatgggattc ccagcacggt
     3181 gagcttgaag ggtatcgtgc cagtgatggc gaacacctag gcgcatttga tcccaaaacg
     3241 ggtaagcagg ttaaagggcc agatccaaaa cggaacatta aaaaatatct ttgagaggta
     3301 aatatggggc ttaaattacg tttgacatgg tttgataaga aaactgaaga gtttaaaggg
     3361 gaagagtatt ctaaagactt tggtgacgat ggttctgtca ttgaaagctt gggaatgcct
     3421 ttaaaggata atattaataa tggtgagttt gatttgaaga aagaatgggt tcctttacta
     3481 cagccatttt ttaaaaatca aataaaacct gatgtggata attgttatat agcttttgat
     3541 tatcgggacg gtaactggta acaaaaaatt atcaaggatg atttgaaata ctgacttccg
     3601 gtgttcaggg atgaatgccg gagtctttaa atacagggaa agtatatgaa aaaggtaaag
     3661 actatttttc tttttatatt gattgttgct ggctttcttc tggccgcatg tcaggcaaat
     3721 tatatccggg atgttcaggg cggaacggtg gcaccatcgt cctcctctga actgacgggg
     3781 atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3841 tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca
     3901 gttggggaac tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc
     3961 cttccagtgt agccggactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca
     4021 aatcctctgc gaactcccag ttaccaatgg ctgctgccag tggcgttttg cgtgctttcc
     4081 cgggttggac tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacggggag
     4141 ttcttgctta cagcccagct tggagcgaac gacctacacc gagccgagat accagtgtgt
     4201 gagctatgag aaagcgccac acttcccgca agggagaacg gcggacaggt atccggtaaa
     4261 cggcagggtc ggaacaggag agcgcaagag ggagcgacct accggaaacg gtggggatct
     4321 ttaagtcctg tcgggtttcg cccgtactgt cagattcatg gttgagcctc acggctccca
     4381 cagatgcacc gaaaaagcgt ctgtttatgt gaactctggc aggagggcgg agcctatgga
     4441 aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg ttagtcccct gcttatccac
     4501 ggaatctgtg ggtaactttg tatgtgtccg cagcgcccgc cgcagtctca cgcccggagc
     4561 gtagcgaccg agtgagcgag gaggcgcaat ttccccggca cctgcaagac ggtcaccgga
     4621 cgcgctgatg tgggatattg tcccctgatt aagtctttct attccctgac gatcaattat
     4681 caggataggt gaaccatgtc ggtttttcgc tgaacatttt acgttaacct atctctgttg
     4741 cttttttctc cttgcggaga aatgcaagtc atccccgctg gggcgtggct gacttcaggg
     4801 gaaaagacct tgcttacgct ggctggacgc caccgacgca cggtcttttc ccctctttct
     4861 ggctctgtcc ggctggacgc cggccacgcc agaaattcac ccggcccccg tcaggctcac
     4921 gcctgaccac ccacaaaaat ccacggtgct ggacgcacct gacgaagcag caaagctgct
     4981 cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc
     5041 ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt
     5101 cctccggggc tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg cggtgaaagc
     5161 tgaacgcaat caccacggac gtgaccgccg ccgccatgcc ttggggcatg tccgcggctt
     5221 gtgcagcggg ggctgcgcat cacgccggta aacccgtcgt ctgaaggaga gagattgcat
     5281 gtttaatacg gattcgcttg cctggcagtg gcatatcccg gagctggccc ggcagattta
     5341 cccgtgggtc agctggctgt accgggagta ttacagtctg gccggttttt ttctggttat
     5401 cggcctgttt ttcggtctgg ctgaactctg ggccggccga atgcgttttg tggggctgag
     5461 ccataaggcg cggcgggagt atgccgatcg ctataagcgc gataataacc ggactgtgcc
     5521 attttcggtg ctggcctcgc tggattcacc cgggcggatg gtcagggcac ggcgacatgt
     5581 ctggaactac gcgtactggc tgaccggcgc tgccggtctg gcccttctgg gggggctggt
     5641 tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgcccggacc tctatgcgaa
     5701 cgcagattat tacctgctgc agccggctaa cctgttcggg atagcctggt gtgccggagg
     5761 gcttgtcgcc ggcgtccttc tggcgcggct gctggcgttc ctgctgcttg atggtcattt
     5821 tgcggccgct gacgaggcgg tgaacgcgaa actgaatcag gagagccggc gcagcagcca
     5881 gcggaccgga gaaatgacag acgtccgcca cctgcatttc ggggagccgg taccggttaa
     5941 cgcgctggcc gatttttcca cggagcaggc ccggaaacag caggcggtgt ttctgggtaa
     6001 ggatgagcag ggccagccgg tgctggtccc tcgtgatacc tggcgcaaga ccaatattca
     6061 gatcctcggc ctgcccggga gcggcaagag cgcgatggcc accaacgcgc tcattcgctg
     6121 cgtacgcgat ttcggggacg ctgtggtgta cttcgacccc aaaggggacg cgtgggcgcc
     6181 gcacgtcttc cgggcgcact gcccggactt caccctgctg gatctgcggc cggggaaacc
     6241 ggcgcagctg aatctgttcc gcgatctcga ccagtacgcc ctgaagaatc tgctggttgc
     6301 cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa
     6361 agcggcgaag ctgattgcgg agcagttccc gcagggggca aacattcagc aggtgctggc
     6421 ggcggcgtac gcgttgccgg aggccctgaa aaaggacgtg aaggggctga tcaccaagct
     6481 ggagaacgtg gccgacctga gcgtcctgca gacggacagc ggtatcgatg tggcggggat
     6541 aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg cggtgatccg
     6601 ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg atgagttccg
     6661 gaggtggccg cacgcgagca tcatgctcga tgagattaaa tatctgctgt cgaagtacgt
     6721 gctgaacgcg ctggggacgc tgcgcagccg ggactgcaac ctgctgctcg cgcaccagtc
     6781 gctgggcgac ttcggtcagt gcgggcagga tctgcccgct gattttgtga aaaccacggt
     6841 actggataac acgccgatcc gctggttcta tcgcgcggcc agccaggagt ccgcgcagtg
     6901 ggcggcgggc cagaccgggg agatccgggt ggacgtggag cgccgccggg ccagccggga
     6961 ggcggggaac gtggagcaca tcagcggcga cagcttcatc cagaaggagg cgcggccgct
     7021 gtttgacgtg aacaccctgc aacacctgcc tgacgggttc gcggtgatga ccgggctggg
     7081 cgtggcgcgg ctggcgttca gcagcccgct gcgggttgag cgccgggaaa tttcgctgaa
     7141 aaccttcccg gtgctggcga aaacggaccc gctggcggag tacctgcctc cggccgacgg
     7201 cggcggacca aaagcggatg atgattttgc ggggctttac tgatgacact ggagcagtac
     7261 aacgtcagcc acgcgaagcg gcaggcgcgg aacgcggaga aaacacggct gaccctgcgc
     7321 tggctgcggg aagagctgtg ttccacggcg gagctggttg cgcgacggct ggggattgcg
     7381 gcggtacagc cggtttaccg cttcctggac agtctggtgg cgaaagggct gctggtccgg
     7441 gcgaaatacc cggttgacgg ccgtcaggtc agcgtgtggg ggctgacgcc gcacggggtg
     7501 gcgttctcgt tcgacgagga cgagccgctg acggacgtta ttccgtttca gccgtcaagg
     7561 gtgtcggcgg cgcagctgcc gcaccggctg gccgtgcagt ccctgcgcct ctccatggag
     7621 ggccgggggg ccaccggctg gcggtacctg caccgtatgg ccctgaaagg gatgaaagtg
     7681 ccggacgcgc tggcggagct ggacggcaga acggtggcat ttgaggtaga gcgcaccgtc
     7741 aagagccgca ggcggtatca ggaagtggtg tccgggtatc tgttcaaccg ccgggcgaac
     7801 ggcatcgatg aaatctggta catctgccct gaccggccga cgcttgtccg ggtacagcgg
     7861 gcgattctgg cggtggatga aatcgtgaat ccacagaccg gcgaggcccg caaaacggtg
     7921 gagctggacc gggagaggct gttcgcgtgc tttaaattta tgacaacgga gtgagggatt
     7981 atggcgctgt acatggtatt tattgaggac gtggaagaca aagacggcga gcgctgcatt
     8041 atgccaacca gcgagcatct ggaagacatg gtggtgctcg cctggcagac cggggcgtat
     8101 gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat cgggatgtat
     8161 gacgagcggc tcaacagcga cgaaaaagcc acgtactttt tcaccggtga aaatccgcaa
     8221 tgggaacggg acaaacccac aggaatgaag ctgtgtgatt acctctatcg aaaggtggag
     8281 cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc accggagcag
     8341 attaacgcga tgattgaggt ggtcagcgag cggcacgggg aaagcgcggt gaacgagaag
     8401 ctgaaagcgg agctgatgcg ggaatataac agccgctatt ttaacgtgcc ggtgcctgaa
     8461 ctgccggagg acaaacccgc ccgccggtgg tggccgttcg gcaaaaaata acgccagcac
     8521 cggcatggcc ggtgctgtta ttcttccccg agcttatccc gcatcacctg ccagagtgtt
     8581 tccgcatcct cgtgaagccg ttcgcacatg tcggccgggg tatccatgtc cagctcgttc
     8641 agcttttcca gcaacagcag cgactgatcc ttaatgaatt tagccatatt gagggcagtt
     8701 ttttcctgct tcgtcatgtt ctccattccc ttcggtaagt tctcagctaa accagccgga
     8761 ccagaagcgt tttttcttct gtgtcagttc tgttgtcaat ctgtccactt cggcctggag
     8821 tttccggcgc tcatcatcgt gctggcggcg ttcttcttcc cgggatgttt tatcttccag
     8881 catcagggtg acagcctgcc ggagatcggc cagttccttt tgcattgcca gaagctgttc
     8941 ctgtgggaaa tttttttgtg tattttcacg gtgtgaaatg ccgggaattt gtttctcagg
     9001 gatattcaca tttccgtata cacggatcag ttctgacaca tccacgacag ggttgttttt
     9061 cccgtcacgt gacacggtaa cctttccctg ttttacatga ttgtatagcg ttcttctggt
     9121 aatgcccgcg gcttgtgctg cctgtgaaag gttgagcaat gttttcgcca ctgtataccc
     9181 ctgattttat cgggtgtgaa aggtgtgtaa tttctcacgg taaatgtatc gctgaatttt
     9241 acccgttttt ccgttcagga tgtggttttc tgacgcgctg cgcttgtcaa accggcgacg
     9301 gccacgcaat gaattgcgcc cccgtcatcg gttattagag gctgtctgcc cccggatacc
     9361 cggattcttt ctgatgcctg acggtgacga tcacaatctg gtccatttcc cggtcgtggc
     9421 ggtagagcat cacatagcca ctgtcgccaa acccgatcac cagttcctta tattccagcg
     9481 gcagaaacgg caccgggcgg ccaatgtctg gcaatgtttt cagttgctgg atagcccgga
     9541 cgatcacctc acctcctttt ctggctgcca gccgattttt ggttttgaga aagtcctgaa
     9601 ggcgttgtaa atcgcgctgt gccagcgcgg aaatcgttac ctgtggcatg gcggagcatc
     9661 ctgctcgttc tcagttcccc aggtacttat ccaggcttcc gcttcttccg cagtaaggtg
     9721 cagacccgtt tcctgatagt gctgccatgc ggcctgtccg tcccgcaggt actggctgcg
     9781 cttctcttcc cggtcgatgt attcggtgat tgcctccagc attagcgcgt gcgctgagcg
     9841 gtgccggtca tcggccagcg ttttcagccg gtccttaagt tcttcatcga gcctgatgga
     9901 tgttgctgat gacataaaag cccctttgta gtcatatgta ttacacatga ctactttaat
     9961 ccgtggcggc gctatttgcc agaaatacgg cttttgctgc ccatttttca gcttcgcttc
    10021 cactaatcag ggctgctaca ggctcactca tcatccagga
//
