LOCUS       NZ_CP066532            11970 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WCHKP090361 plasmid p2_090361, complete
            sequence.
ACCESSION   NZ_CP066532
VERSION     NZ_CP066532.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15649156
            Assembly: GCF_014041645.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 090361
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Wei,L., Wen,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 02:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,858
            CDSs (total)                      :: 5,737
            Genes (coding)                    :: 5,571
            CDSs (with protein)               :: 5,571
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 59 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 19 of 166
            Pseudo Genes (multiple problems)  :: 23 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066532.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP090361"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_090361"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-06-19"
     gene            complement(1414..1563)
                     /locus_tag="H3G66_RS28930"
                     /old_locus_tag="H3G66_028930"
     CDS             complement(1414..1563)
                     /locus_tag="H3G66_RS28930"
                     /old_locus_tag="H3G66_028930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1648..1905)
                     /locus_tag="H3G66_RS28935"
                     /old_locus_tag="H3G66_028935"
     CDS             complement(1648..1905)
                     /locus_tag="H3G66_RS28935"
                     /old_locus_tag="H3G66_028935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1915..3600)
                     /locus_tag="H3G66_RS28940"
                     /old_locus_tag="H3G66_028940"
     CDS             complement(1915..3600)
                     /locus_tag="H3G66_RS28940"
                     /old_locus_tag="H3G66_028940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(3705..5192)
                     /gene="ltrA"
                     /locus_tag="H3G66_RS28945"
                     /old_locus_tag="H3G66_028945"
     CDS             complement(3705..5192)
                     /gene="ltrA"
                     /locus_tag="H3G66_RS28945"
                     /old_locus_tag="H3G66_028945"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            5837..6082
                     /locus_tag="H3G66_RS28950"
                     /old_locus_tag="H3G66_028950"
     CDS             5837..6082
                     /locus_tag="H3G66_RS28950"
                     /old_locus_tag="H3G66_028950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6356..6727
                     /gene="tolA"
                     /locus_tag="H3G66_RS28955"
                     /old_locus_tag="H3G66_028955"
     CDS             6356..6727
                     /gene="tolA"
                     /locus_tag="H3G66_RS28955"
                     /old_locus_tag="H3G66_028955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6724..7089
                     /locus_tag="H3G66_RS29395"
     CDS             6724..7089
                     /locus_tag="H3G66_RS29395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7264..7542
                     /locus_tag="H3G66_RS28965"
                     /old_locus_tag="H3G66_028965"
     CDS             7264..7542
                     /locus_tag="H3G66_RS28965"
                     /old_locus_tag="H3G66_028965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7530..7844
                     /locus_tag="H3G66_RS28970"
                     /old_locus_tag="H3G66_028970"
     CDS             7530..7844
                     /locus_tag="H3G66_RS28970"
                     /old_locus_tag="H3G66_028970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8008..8436
                     /locus_tag="H3G66_RS28975"
                     /old_locus_tag="H3G66_028975"
     CDS             8008..8436
                     /locus_tag="H3G66_RS28975"
                     /old_locus_tag="H3G66_028975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8462..8641
                     /locus_tag="H3G66_RS28980"
                     /old_locus_tag="H3G66_028980"
     CDS             8462..8641
                     /locus_tag="H3G66_RS28980"
                     /old_locus_tag="H3G66_028980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8668..9198)
                     /locus_tag="H3G66_RS28985"
                     /old_locus_tag="H3G66_028985"
     CDS             complement(8668..9198)
                     /locus_tag="H3G66_RS28985"
                     /old_locus_tag="H3G66_028985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9205..9936)
                     /gene="mobC"
                     /locus_tag="H3G66_RS28990"
                     /old_locus_tag="H3G66_028990"
     CDS             complement(9205..9936)
                     /gene="mobC"
                     /locus_tag="H3G66_RS28990"
                     /old_locus_tag="H3G66_028990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(9936..11900)
                     /locus_tag="H3G66_RS28995"
                     /old_locus_tag="H3G66_028995"
     CDS             complement(9936..11900)
                     /locus_tag="H3G66_RS28995"
                     /old_locus_tag="H3G66_028995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8600
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          733..1311
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9209..9936)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg
       61 gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac
      121 gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc
      181 cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct
      241 gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg
      301 ggggccgggt gaatttctgg cgtggccggc gtccagccgg acagagccag aaagagggga
      361 aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag gtcttttccc ctgaagtcag
      421 ccacgcccca gcggggatga cttgcatttc tccgcaagga gaaaaaagca acagagatag
      481 gttaacgtaa aatgttcagc gaaaaaccga catggttcac ctatcctgat aattgatcgt
      541 cagggaatag aaagacttaa tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt
      601 cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg
      661 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
      721 gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc cataggctcc
      781 gccctcctgc cagagttcac ataaacagac gctttttcgg tgcatctgtg ggagccgtga
      841 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
      901 tttccggtag gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
      961 ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct catagctcac acactggtat
     1021 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag
     1081 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccggg aaagcacgca
     1141 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     1201 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     1261 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     1321 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     1381 aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc
     1441 agaggaggac gatggtgcca ccgttccgcc ctgaacatcc cggatataat ttgcctgaca
     1501 tgcggccaga agaaagccag caacaatcaa tataaaaaga aaaatagtct ttaccttttt
     1561 catatacttt ccctgtattt aaagactccg gcattcatcc ctgaacaccg gaagtcagta
     1621 tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat caaaagctat
     1681 ataacaatta tccacatcag gttttatttg atttttaaaa aatggctgta gtaaaggaac
     1741 ccattctttc ttcaaatcaa actcaccatt attaatatta tcctttaaag gcattcccaa
     1801 gctttcaatg acagaaccat cgtcaccaaa gtctttagaa tactcttccc ctttaaactc
     1861 ttcagttttc ttatcaaacc atgtcaaacg taatttaagc cccatattta cctctcaaag
     1921 atatttttta atgttccgtt ttggatctgg ccctttaacc tgcttacccg ttttgggatc
     1981 aaatgcgcct aggtgttcgc catcactggc acgataccct tcaagctcac cgtgctggga
     2041 atcccattca taaattttac gttttttatc gccataccat cgggcccgct taccaccacc
     2101 accttgttta ggtgttttag gatcacctcg ctttaactct ccaagtccct ttatgtcttc
     2161 tgttttaggt actggatgat aatcatggcc gtagtcttta gtccccttgc gaggcttgtt
     2221 cttttcctca tttaacttat tttctgcatc cttcgctttt ttctcctttt gtttccggct
     2281 ctccatcgcc ccggtaagtg cagccttatc tctggataag gattcattca ttgcgtttaa
     2341 agatgcctga gccgctttta atttctcatt ctgattctga atatccttat caagatagcg
     2401 aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca caaaatcatc
     2461 tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag cccttgcacc
     2521 ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat ttttatcttc
     2581 ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt ccggatgagc
     2641 ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta cctgtgccgg
     2701 ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt tcgggaaacg
     2761 aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg aatcaccttt
     2821 ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg gtacgctcac
     2881 ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt ttgtcggttt
     2941 cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat gctgccgctc
     3001 atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt ccagtggtaa
     3061 agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa tttttgacat
     3121 cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg caatccccca
     3181 cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg cggccgacaa
     3241 cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca gggcaggcag
     3301 accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc tatgattgcc
     3361 gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc ccggattata
     3421 gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc
     3481 cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat tcccactggc
     3541 ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc caccactcat
     3601 accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatgtcg cggtagggat
     3661 acccgttacc ggataccccc cgcacagatc ccggcgtgcg cgatttacgc accgggctcc
     3721 tgcctcgggt gtctggcggt gaaccgctcc acaggccatg gatgaagaac ccgaaccctt
     3781 ggtagccatg cggctgccag tttgtttgct ttcgtccagg tcgtatcatc cttctggctc
     3841 ctgcgcctga gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt ctgcatggtg
     3901 gggaagttgc ccggtaccga gtgatagttc aggtatccct gaaccactct cctgagccat
     3961 tttccctgtt cggggattga gtaatgccag cgccttcgca gaccgtcttt gatggctttc
     4021 agagttgccg tcatccgatc ccggcgggtc tttcgtatca gcatgaacct gccgttgcga
     4081 tctttcccgc tgatgtgcgt gaacccgagg aagttgaacg tttctggttt gccttttccc
     4141 ctgatggcac ggttttcggc agcgaagcgg ccgaactcca tcagacgggt tttctccggg
     4201 tgaaccgtga gtccgaactc cctcagtctg cgctgcatgg ctatacggaa gcgccgggca
     4261 tcgtatcgtt tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat taccacattg
     4321 cctgtggcat agcgacgtcg ccactgatgc gcccacagat cgaagacgta gtggaggtat
     4381 atgtttgcca gcagcggtga gatgaccgca ccctgtgggg tgccttcctc cgttgctcgc
     4441 cattgaccct cctccgacgt cccggctgtg agccacttac gtatgagcct gattaccctc
     4501 cggtcgccga tccgatgctc tgtgaacctg atcagccatt cgtggctcac cctgtcgaag
     4561 aactgactga tgtcggcatc cagtacccag tttacgttag tgcgtaccag ccctgtggcc
     4621 agtgcgtcca gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga gaaccccata
     4681 aagtcgtttt catagactgc gttcaggatt ttcaccagcg catactggac gatcttgtcc
     4741 tccagcgagg cgatgccgag cgggcgttgt tttccatccg cttttgggat gtagtgacgc
     4801 ctgccgggct gcgccctgta gctgccctga tgtagcctcc ggtgcagatc tgttatgttg
     4861 ttcttcatgt ttccggcgta gtccatccac ctgatgccat ccactccggc ggccgctttc
     4921 ctgctcaggg agaggaatgc ggcttccagt gcttcgactg tcagcaggtg gaacaatgct
     4981 gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag cctctgtgac
     5041 atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc cttggccccg
     5101 ctccttcgct ccactgactc cgctcctttc gggttgttcg cctgcttcgc cgctactatg
     5161 agcgagtccg acttctcctc tccgtacatc accggctatg actcctcgtc ttcccggtgc
     5221 gggccatctc cgacactggc agatggtcag aggggagatc tcccggttcc cgcgtagaga
     5281 tcgtattgac atgccagggt ctcagacccc gccgggtcca tgtggcactc gcagtatcgc
     5341 accctatgat gttgccttcc gttaacagta caacgtcggc acccggtaat ttaatataca
     5401 tttcgtggct caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc catacctcgc
     5461 ggtatgcaac gcatgactcg gggaccttgt ggattgctgg tccttcaatg gtcggggact
     5521 ttcacccctt gatctctaac cggtctcccg gcgcacactg tacatataca cagtataatt
     5581 attaccttac tcctcccgtt ttcaggtgtc aatatatgag attagttctc atcttcaggc
     5641 acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa aatttctcat
     5701 ggcaacaaat tttattattt aagacattca tcacacttac atttcagatg gtatagacac
     5761 atctgaaagc taactggcat gaggagagga cctgcggtta aactgttcgg gaaacctgag
     5821 acgcccggag gccactatga cgcacctgaa actgactccc gctgaagaaa agcgcctggc
     5881 ggacgccttt gtcgctcagg cactggccat gggcctgaaa gggaacaaaa cgctgccaaa
     5941 ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc attcaggacg aacgtgaagc
     6001 caggcgggcg caggaaatgc gtcgggagaa atacgaggct acggaaacca ccttcacctg
     6061 gcagccacag cgccgccggt aaggggtatt taccataacg tcgcattgcc cgcaccgctc
     6121 agacggtctg agggcgtttc cggcggctac gggataaaac aggcaacaaa ccacccggaa
     6181 aaccgccgga aacgcgcccg gtaaattgta acccgctgca tgcctatgca cgcaactgca
     6241 tggcgggccg tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa tgaacgtggc
     6301 cgtaaagagt gacaaatata catgtaacat tttttactga caggtcatca acccgatgcg
     6361 tttaataccc tttatcctgc tggcattgtg cagcccctta ctcctgacgg cctgtaagca
     6421 ttccaccacc cagccgccga aaatttatac tctggataag tcatctaaaa tcagtagcta
     6481 cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt gaaccagagg cctatgcggg
     6541 aaaaatgtgc accgtgcgga tatccataca acgtgatggc actctgaaca gcgccattgc
     6601 tgaaggagga gatccgaaac tctgtaaagc agcaatatca gccgttaccc acgccaaaat
     6661 tccgccggca ccggatgaaa atacctggca aatattcaaa aacgcccctc tggatttcag
     6721 accatgaaag cttttactct gacagtgctg gcggccatgt tcctcactcc ggtacatgcg
     6781 ataccactaa aacccgtaac cacactgcag ccaacctacc cggtacaggc cgcagcaaaa
     6841 cgcatcgaag gtcaggtgac cgccacgttt gatattctgg acgatggacg agtggacaac
     6901 gtgtcgctta ccggctgtca cctttttttt cgggaaacgc tgacggcaat gaggcactgg
     6961 ctatatcccg cagggcaccc ggcaatgcga caggttataa attttgaatt cagtctgaaa
     7021 aatgacggct tacaggatcc gcgaccagtc accacccaaa agctcaatga tgatggttgc
     7081 cgccagtaac aggtccttta acccgcaggc tgcaatagtc ctggatgatg agtgagcctg
     7141 tagcagccct gattagtgga agcgaagctg aaaaatgggc agcaaaagcc gtatttctgg
     7201 caaatagcgc cgccacggat taaagtagtc atgtgtaata catatgacta caaaggggct
     7261 tttatgtcat cagcaacatc catcaggctc gatgaagaac ttaaggaccg gctgaaaacg
     7321 ctggccgatg accggcaccg ctcagcgcac gcgctaatgc tggaggcaat caccgaatac
     7381 atcgaccggg aagagaagcg cagccagtac ctgcgggacg gacaggccgc atggcagcac
     7441 tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg aagcctggat aagtacctgg
     7501 ggaactgaga acgagcagga tgctccgcca tgccacaggt aacgatttcc gcgctggcac
     7561 agcgcgattt acaacgcctt caggactttc tcaaaaccaa aaatcggctg gcagccagaa
     7621 aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa aacattgcca gacattggcc
     7681 gcccggtgcc gtttctgccg ctggaatata aggaactggt gatcgggttt ggcgacagtg
     7741 gctatgtgat gctctaccgc cacgaccggg aaatggacca gattgtgatc gtcaccgtca
     7801 ggcatcagaa agaatccggg tatccggggg cagacagcct ctaataaccg atgacggggg
     7861 cgcaattcat tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc
     7921 ctgaacggaa aaacgggtaa aattcagcga tacatttacc gtgagaaatt acacaccttt
     7981 cacacccgat aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct ttcacaggca
     8041 gcacaagccg cgggcattac cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt
     8101 accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact gatccgtgta
     8161 tacggaaatg tgaatatccc tgagaaacaa attcccggca tttcacaccg tgaaaataca
     8221 caaaaaaatt tcccacagga acagcttctg gcaatgcaaa aggaactggc cgatctccgg
     8281 caggctgtca ccctgatgct ggaagataaa acatcccggg aagaagaacg ccgccagcac
     8341 gatgatgagc gccggaaact ccaggccgaa gtggacagat tgacaacaga actgacacag
     8401 aagaaaaaac gcttctggtc cggctggttt agctgagaac ttaccgaagg gaatggagaa
     8461 catgacgaag caggaaaaaa ctgccctcaa tatggctaaa ttcattaagg atcagtcgct
     8521 gctgttgctg gaaaagctga acgagctgga catggatacc ccggccgaca tgtgcgaacg
     8581 gcttcacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaagaata
     8641 acagcaccgg ccatgccggt gctggcgtta ttttttgccg aacggccacc accggcgggc
     8701 gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg
     8761 catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac
     8821 ctcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc
     8881 gttctcgatg gcgaaccgct ccacctttcg atagaggtaa tcacacagct tcattcctgt
     8941 gggtttgtcc cgttcccatt gcggattttc accggtgaaa aagtacgtgg ctttttcgtc
     9001 gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg
     9061 gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca tgtcttccag
     9121 atgctcgctg gttggcataa tgcagcgctc gccgtctttg tcttccacgt cctcaataaa
     9181 taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc acgcgaacag
     9241 cctctcccgg tccagctcca ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc
     9301 atccaccgcc agaatcgccc gctgtacccg gacaagcgtc ggccggtcag ggcagatgta
     9361 ccagatttca tcgatgccgt tcgcccggcg gttgaacaga tacccggaca ccacttcctg
     9421 ataccgcctg cggctcttga cggtgcgctc tacctcaaat gccaccgttc tgccgtccag
     9481 ctccgccagc gcgtccggca ctttcatccc tttcagggcc atacggtgca ggtaccgcca
     9541 gccggtggcc ccccggccct ccatggagag gcgcagggac tgcacggcca gccggtgcgg
     9601 cagctgcgcc gccgacaccc ttgacggctg aaacggaata acgtccgtca gcggctcgtc
     9661 ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc
     9721 gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc accagactgt ccaggaagcg
     9781 gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca accagctccg ccgtggaaca
     9841 cagctcttcc cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg
     9901 cttcgcgtgg ctgacgttgt actgctccag tgtcatcagt aaagccccgc aaaatcatca
     9961 tccgcttttg gtccgccgcc gtcggccgga ggcaggtact ccgccagcgg gtccgttttc
    10021 gccagcaccg ggaaggtttt cagcgaaatt tcccggcgct caacccgcag cgggctgctg
    10081 aacgccagcc gcgccacgcc cagcccggtc atcaccgcga acccgtcagg caggtgttgc
    10141 agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc gccgctgatg
    10201 tgctccacgt tccccgcctc ccggctggcc cggcggcgct ccacgtccac ccggatctcc
    10261 ccggtctggc ccgccgccca ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg
    10321 atcggcgtgt tatccagtac cgtggttttc acaaaatcag cgggcagatc ctgcccgcac
    10381 tgaccgaagt cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc
    10441 gtccccagcg cgttcagcac gtacttcgac agcagatatt taatctcatc gagcatgatg
    10501 ctcgcgtgcg gccacctccg gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca
    10561 aacagcatct tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg
    10621 cagccgccgc cgttgattat ccccgccaca tcgataccgc tgtccgtctg caggacgctc
    10681 aggtcggcca cgttctccag cttggtgatc agccccttca cgtccttttt cagggcctcc
    10741 ggcaacgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc
    10801 gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc
    10861 tcactcaggt taaacccggc aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg
    10921 aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccgggcag
    10981 tgcgcccgga agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta caccacagcg
    11041 tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc
    11101 ccgggcaggc cgaggatctg aatattggtc ttgcgccagg tatcacgagg gaccagcacc
    11161 ggctggccct gctcatcctt acccagaaac accgcctgct gtttccgggc ctgctccgtg
    11221 gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg gcggacgtct
    11281 gtcatttctc cggtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc
    11341 gcctcgtcag cggccgcaaa atgaccatca agcagcagga acgccagcag ccgcgccaga
    11401 aggacgccgg cgacaagccc tccggcacac caggctatcc cgaacaggtt agccggctgc
    11461 agcaggtaat aatctgcgtt cgcatagagg tccgggcgca ggacggtttc tccccagctc
    11521 tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggcagc gccggtcagc
    11581 cagtacgcgt agttccagac atgtcgccgt gccctgacca tccgcccggg tgaatccagc
    11641 gaggccagca ccgaaaatgg cacagtccgg ttattatcgc gcttatagcg atcggcatac
    11701 tcccgccgcg ccttatggct cagccccaca aaacgcattc ggccggccca gagttcagcc
    11761 agaccgaaaa acaggccgat aaccagaaaa aaaccggcca gactgtaata ctcccggtac
    11821 agccagctga cccacgggta aatctgccgg gccagctccg ggatatgcca ctgccaggca
    11881 agcgaatccg tattaaacat gcaatctctc tccttcagac gacgggttta ccggcgtgat
    11941 gcgcagcccc cgctgcacaa gccgcggaca
//
