LOCUS       NZ_CP066526            10060 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WCHKP090357 plasmid p2_090357, complete
            sequence.
ACCESSION   NZ_CP066526
VERSION     NZ_CP066526.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15649152
            Assembly: GCF_014041735.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 090357
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Wei,L., Wen,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 02:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,851
            CDSs (total)                      :: 5,730
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 56 of 148
            Pseudo Genes (incomplete)         :: 96 of 148
            Pseudo Genes (internal stop)      :: 18 of 148
            Pseudo Genes (multiple problems)  :: 22 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066526.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP090357"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_090357"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-06-11"
     gene            complement(29..394)
                     /locus_tag="H3G71_RS29340"
     CDS             complement(29..394)
                     /locus_tag="H3G71_RS29340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(391..762)
                     /gene="tolA"
                     /locus_tag="H3G71_RS28925"
                     /old_locus_tag="H3G71_028925"
     CDS             complement(391..762)
                     /gene="tolA"
                     /locus_tag="H3G71_RS28925"
                     /old_locus_tag="H3G71_028925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(1036..1281)
                     /locus_tag="H3G71_RS28930"
                     /old_locus_tag="H3G71_028930"
     CDS             complement(1036..1281)
                     /locus_tag="H3G71_RS28930"
                     /old_locus_tag="H3G71_028930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1608..3293
                     /locus_tag="H3G71_RS28935"
                     /old_locus_tag="H3G71_028935"
     CDS             1608..3293
                     /locus_tag="H3G71_RS28935"
                     /old_locus_tag="H3G71_028935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3303..3560
                     /locus_tag="H3G71_RS28940"
                     /old_locus_tag="H3G71_028940"
     CDS             3303..3560
                     /locus_tag="H3G71_RS28940"
                     /old_locus_tag="H3G71_028940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3645..3794
                     /locus_tag="H3G71_RS28945"
                     /old_locus_tag="H3G71_028945"
     CDS             3645..3794
                     /locus_tag="H3G71_RS28945"
                     /old_locus_tag="H3G71_028945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5278..7242
                     /locus_tag="H3G71_RS28950"
                     /old_locus_tag="H3G71_028950"
     CDS             5278..7242
                     /locus_tag="H3G71_RS28950"
                     /old_locus_tag="H3G71_028950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7242..7973
                     /gene="mobC"
                     /locus_tag="H3G71_RS28955"
                     /old_locus_tag="H3G71_028955"
     CDS             7242..7973
                     /gene="mobC"
                     /locus_tag="H3G71_RS28955"
                     /old_locus_tag="H3G71_028955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7980..8510
                     /locus_tag="H3G71_RS28960"
                     /old_locus_tag="H3G71_028960"
     CDS             7980..8510
                     /locus_tag="H3G71_RS28960"
                     /old_locus_tag="H3G71_028960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8537..8716)
                     /locus_tag="H3G71_RS28965"
                     /old_locus_tag="H3G71_028965"
     CDS             complement(8537..8716)
                     /locus_tag="H3G71_RS28965"
                     /old_locus_tag="H3G71_028965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8742..9170)
                     /locus_tag="H3G71_RS28970"
                     /old_locus_tag="H3G71_028970"
     CDS             complement(8742..9170)
                     /locus_tag="H3G71_RS28970"
                     /old_locus_tag="H3G71_028970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(9334..9648)
                     /locus_tag="H3G71_RS28975"
                     /old_locus_tag="H3G71_028975"
     CDS             complement(9334..9648)
                     /locus_tag="H3G71_RS28975"
                     /old_locus_tag="H3G71_028975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9636..9914)
                     /locus_tag="H3G71_RS28980"
                     /old_locus_tag="H3G71_028980"
     CDS             complement(9636..9914)
                     /locus_tag="H3G71_RS28980"
                     /old_locus_tag="H3G71_028980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     BGC             1..8293
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3898..4476)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7243..7970
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tattgcagcc tgcgggttaa aggacctgtt actggcggca accatcatca ttgagctttt
       61 gggtggtgac tggtcgcgga tcctgtaagc cgtcattttt cagactgaat tcaaaattta
      121 taacctgtcg cattgccggg tgccctgcgg gatatagcca gtgcctcatt gccgtcagcg
      181 tttcccgaaa aaaaaggtga cagccggtaa gcgacacgtt gtccactcgt ccatcgtcca
      241 gaatatcaaa cgtggcggtc acctgacctt cgatgcgttt tgctgcggcc tgtaccgggt
      301 aggttggctg cagtgtggtt acgggtttta gtggtatcgc atgtaccgga gtgaggaaca
      361 tggccgccag cactgtcaga gtaaaagctt tcatggtctg aaatccagag gggcgttttt
      421 gaatatttgc caggtatttt catccggtgc cggcggaatt ttggcgtggg taacggctga
      481 tattgctgct ttacagagtt tcggatctcc tccttcagca atggcgctgt tcagagtgcc
      541 atcacgttgt atggatatcc gcacggtgca catttttccc gcataggcct ctggttcaaa
      601 catctgcatt tcaacagcgg tcttgatggc cgcagcgtag ctactgattt tagatgactt
      661 atccagagta taaattttcg gcggctgggt ggtggaatgc ttacaggccg tcaggagtaa
      721 ggggctgcac aatgccagca ggataaaggg tattaaacgc atcgggttga tgacctgtca
      781 gtaaaaaatg ttacatgtat atttgtcact ctttacggcc acgttcattt acataaacac
      841 ggcttatcgt atcgttttta caccaaacgg cccgccatgc agttgcgtgc ataggcatgc
      901 agcgggttac aatttaccgg gcgcgtttcc ggcggttttc cgggtggttt gttgcctgtt
      961 ttatcccgta gccgccggaa acgccctcag accgtctgag cggtgcgggc aatgcgacgt
     1021 tatggtaaat accccttacc ggcggcgctg tggctgccag gtgaaggtgg tttccgtagc
     1081 ctcgtatttc tcccgacgca tttcctgcgc ccgcctggct tcacgttcgt cctgaatggc
     1141 acgaagcacc ctttcccgca cctgttgttt taccgccttt ggcagcgttt tgttcccttt
     1201 caggcccatg gccagtgcct gagcgacaaa ggcgtccgcc aggcgctttt cttcagcggg
     1261 agtcagtttc aggtgcgtca tagtggcctc cgggcgtctc aggtttcccg aacagtttaa
     1321 ccgcaggtcc tctcctcatg ccagttagct ttcagatgtg tctataccat ctgaaatgta
     1381 agtgtgatga atgtcttaaa taataaaatt tgttgccatg agaaattttc gggaaaagac
     1441 gcgatctgct tacaggtggc tccgggcgct tttttgtgcc tgaagatgag aactaatctc
     1501 atatattgac acctgaaaac gggaggagta aggtaataat tatactgtgt atatgtacag
     1561 tatgttggct ttaatttatt gttttaaatg tcaaagagga aaacggtatg agtggtggag
     1621 acggtcgagg tccgggtaat tcaggtctgg gacataatgg tggtcaggcc agtgggaatg
     1681 tgaacggtac gtctggtaaa ggtggccctt catcaggtgg tggtacggat ccaaacagcg
     1741 ggccgggctg gggtacgacg catacgccga atggagatat tcataactat aatccggggg
     1801 agtttggtaa cggcgggagt aaacccggtg gtaatggtgg taacagcggc aatcatagcg
     1861 gtagctctgg tggcggacag tcttcggcca ctgcgatggc cttcggtctg cctgccctgg
     1921 ctactccggg ggctgaagga ctggctttat cagtttccgg cgatgcgttg tcggccgccg
     1981 ttgctgatgt gctggctgcc ctgaaggggc cgtttaagtt tggtctgtgg gggattgcga
     2041 tctacggtgt gctgccttct gagattgcaa aagatgatcc gaaaatgatg tcaaaaatta
     2101 tgacgtcatt accggccgat gcggtgacgg agactccggc aagtacttta ccactggacc
     2161 aggcgacggt tcgtgtcaga caacgggttg tggatgtggt gaaggatgag cggcagcata
     2221 ttgcggttgt cgcaggtcgg ccaatgagtg tccctgtggt ggatgcgaaa ccgacaaaac
     2281 gtccgggggt attcagtgtg tcgattccgg gtctcccgtc tctgcaggtg agcgtaccga
     2341 aaggtgttcc gacagcgaaa gccccgccaa aaggcattgt tgctgaaaaa ggtgattcac
     2401 gtccggctgg ttttacagcc ggtggtaact cccgtgaggc cgttattcgt ttcccgaaag
     2461 agaccggaca gaagccggtt tatgtgtcgg tgacagatgt tcttaccccg gcacaggtaa
     2521 aacagcgtca ggaggaagaa aagcgtcgcc agcaggcatg ggacgccgct catccggaag
     2581 aggggctgaa aagagactat gataaagcga aagctgagct ggatgccgaa gataaaaata
     2641 ttgcgacctt aaacagccgc attgcatcga cagagaaggc gctccccggt gcaagggctg
     2701 ctgttcagga agccgataaa aaggtaaaag aggcagaggc aaataaagat gattttgtga
     2761 cttataaccc gcctcatgaa tatggctccg ggtggcagga tcaggttcgc tatcttgata
     2821 aggatattca gaatcagaat gagaaattaa aagcggctca ggcatcttta aacgcaatga
     2881 atgaatcctt atccagagat aaggctgcac ttaccggggc gatggagagc cggaaacaaa
     2941 aggagaaaaa agcgaaggat gcagaaaata agttaaatga ggaaaagaac aagcctcgca
     3001 aggggactaa agactacggc catgattatc atccagtacc taaaacagaa gacataaagg
     3061 gacttggaga gttaaagcga ggtgatccta aaacacctaa acaaggtggt ggtggtaagc
     3121 gggcccgatg gtatggcgat aaaaaacgta aaatttatga atgggattcc cagcacggtg
     3181 agcttgaagg gtatcgtgcc agtgatggcg aacacctagg cgcatttgat cccaaaacgg
     3241 gtaagcaggt taaagggcca gatccaaaac ggaacattaa aaaatatctt tgagaggtaa
     3301 atatggggct taaattacgt ttgacatggt ttgataagaa aactgaagag tttaaagggg
     3361 aagagtattc taaagacttt ggtgacgatg gttctgtcat tgaaagcttg ggaatgcctt
     3421 taaaggataa tattaataat ggtgagtttg atttgaagaa agaatgggtt cctttactac
     3481 agccattttt taaaaatcaa ataaaacctg atgtggataa ttgttatata gcttttgatt
     3541 atcgggacgg taactggtaa caaaaaatta tcaaggatga tttgaaatac tgacttccgg
     3601 tgttcaggga tgaatgccgg agtctttaaa tacagggaaa gtatatgaaa aaggtaaaga
     3661 ctatttttct ttttatattg attgttgctg gctttcttct ggccgcatgt caggcaaatt
     3721 atatccggga tgttcagggc ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga
     3781 tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3841 cttttgccct gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag
     3901 ttggggaact gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc
     3961 ttccagtgta gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa
     4021 atcctctgcg aactcccagt taccaatggc tgctgccagt ggcgttttgc gtgctttccc
     4081 gggttggact caagtgaaca gttaccggat aaggcgcagc agtcgggctg aacggggagt
     4141 tcttgcttac agcccagctt ggagcgaacg acctacaccg agccgagata ccagtgtgtg
     4201 agctatgaga aagcgccaca cttcccgcaa gggagaacgg cggacaggta tccggtaaac
     4261 ggcagggtcg gaacaggaga gcgcaagagg gagcgaccta ccggaaacgg tggggatctt
     4321 taagtcctgt cgggtttcgc ccgtactgtc agattcatgg ttgagcctca cggctcccac
     4381 agatgcaccg aaaaagcgtc tgtttatgtg aactctggca ggagggcgga gcctatggaa
     4441 aaacgccacc ggcgcggccc tgccgttttg cctcacatgt tagtcccctg cttatccacg
     4501 gaatctgtgg gtaactttgt atgtgtccgc agcgcccgcc gcagtctcac gcccggagcg
     4561 tagcgaccga gtgagcgagg aggcgcaatt tccccggcac ctgcaagacg gtcaccggac
     4621 gcgctgatgt gggatattgt cccctgatta agtctttcta ttccctgacg atcaattatc
     4681 aggataggtg aaccatgtcg gtttttcgct gaacatttta cgttaaccta tctctgttgc
     4741 ttttttctcc ttgcggagaa atgcaagtca tccccgctgg ggcgtggctg acttcagggg
     4801 aaaagacctt gcttacgctg gctggacgcc accgacgcac ggtcttttcc cctctttctg
     4861 gctctgtccg gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg
     4921 cctgaccacc cacaaaaatc cacggtgctg gacgcacctg acgaagcagc aaagctgctc
     4981 ctgattttgc gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg
     5041 gaaaagaccg cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc
     5101 ctccggggct gcgccttttc cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct
     5161 gaacgcaatc accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg
     5221 tgcagcgggg gctgcgcatc acgccggtaa acccgtcgtc tgaaggagag agattgcatg
     5281 tttaatacgg attcgcttgc ctggcagtgg catatcccgg agctggcccg gcagatttac
     5341 ccgtgggtca gctggctgta ccgggagtat tacagtctgg ccggtttttt tctggttatc
     5401 ggcctgtttt tcggtctggc tgaactctgg gccggccgaa tgcgttttgt ggggctgagc
     5461 cataaggcgc ggcgggagta tgccgatcgc tataagcgcg ataataaccg gactgtgcca
     5521 ttttcggtgc tggcctcgct ggattcaccc gggcggatgg tcagggcacg gcgacatgtc
     5581 tggaactacg cgtactggct gaccggcgct gccggtctgg cccttctggg ggggctggtt
     5641 gtcgccgtgt ttatcgagag ctggggagaa accgtcctgc gcccggacct ctatgcgaac
     5701 gcagattatt acctgctgca gccggctaac ctgttcggga tagcctggtg tgccggaggg
     5761 cttgtcgccg gcgtccttct ggcgcggctg ctggcgttcc tgctgcttga tggtcatttt
     5821 gcggccgctg acgaggcggt gaacgcgaaa ctgaatcagg agagccggcg cagcagccag
     5881 cggaccggag aaatgacaga cgtccgccac ctgcatttcg gggagccggt accggttaac
     5941 gcgctggccg atttttccac ggagcaggcc cggaaacagc aggcggtgtt tctgggtaag
     6001 gatgagcagg gccagccggt gctggtccct cgtgatacct ggcgcaagac caatattcag
     6061 atcctcggcc tgcccgggag cggcaagagc gcgatggcca ccaacgcgct cattcgctgc
     6121 gtacgcgatt tcggggacgc tgtggtgtac ttcgacccca aaggggacgc gtgggcgccg
     6181 cacgtcttcc gggcgcactg cccggacttc accctgctgg atctgcggcc ggggaaaccg
     6241 gcgcagctga atctgttccg cgatctcgac cagtacgccc tgaagaatct gctggttgcc
     6301 gggtttaacc tgagtgagac cggcgacgtg gcggaccact accgcatcag cgagcagaaa
     6361 gcggcgaagc tgattgcgga gcagttcccg cagggggcaa acattcagca ggtgctggcg
     6421 gcggcgtacg cgttgccgga ggccctgaaa aaggacgtga aggggctgat caccaagctg
     6481 gagaacgtgg ccgacctgag cgtcctgcag acggacagcg gtatcgatgt ggcggggata
     6541 atcaacggcg gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg
     6601 gtgcagaaga tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga tgagttccgg
     6661 aggtggccgc acgcgagcat catgctcgat gagattaaat atctgctgtc gaagtacgtg
     6721 ctgaacgcgc tggggacgct gcgcagccgg gactgcaacc tgctgctcgc gcaccagtcg
     6781 ctgggcgact tcggtcagtg cgggcaggat ctgcccgctg attttgtgaa aaccacggta
     6841 ctggataaca cgccgatccg ctggttctat cgcgcggcca gccaggagtc cgcgcagtgg
     6901 gcggcgggcc agaccgggga gatccgggtg gacgtggagc gccgccgggc cagccgggag
     6961 gcggggaacg tggagcacat cagcggcgac agcttcatcc agaaggaggc gcggccgctg
     7021 tttgacgtga acaccctgca acacctgcct gacgggttcg cggtgatgac cgggctgggc
     7081 gtggcgcggc tggcgttcag cagcccgctg cgggttgagc gccgggaaat ttcgctgaaa
     7141 accttcccgg tgctggcgaa aacggacccg ctggcggagt acctgcctcc ggccgacggc
     7201 ggcggaccaa aagcggatga tgattttgcg gggctttact gatgacactg gagcagtaca
     7261 acgtcagcca cgcgaagcgg caggcgcgga acgcggagaa aacacggctg accctgcgct
     7321 ggctgcggga agagctgtgt tccacggcgg agctggttgc gcgacggctg gggattgcgg
     7381 cggtacagcc ggtttaccgc ttcctggaca gtctggtggc gaaagggctg ctggtccggg
     7441 cgaaataccc ggttgacggc cgtcaggtca gcgtgtgggg gctgacgccg cacggggtgg
     7501 cgttctcgtt cgacgaggac gagccgctga cggacgttat tccgtttcag ccgtcaaggg
     7561 tgtcggcggc gcagctgccg caccggctgg ccgtgcagtc cctgcgcctc tccatggagg
     7621 gccggggggc caccggctgg cggtacctgc accgtatggc cctgaaaggg atgaaagtgc
     7681 cggacgcgct ggcggagctg gacggcagaa cggtggcatt tgaggtagag cgcaccgtca
     7741 agagccgcag gcggtatcag gaagtggtgt ccgggtatct gttcaaccgc cgggcgaacg
     7801 gcatcgatga aatctggtac atctgccctg accggccgac gcttgtccgg gtacagcggg
     7861 cgattctggc ggtggatgaa atcgtgaatc cacagaccgg cgaggcccgc aaaacggtgg
     7921 agctggaccg ggagaggctg ttcgcgtgct ttaaatttat gacaacggag tgagggatta
     7981 tggcgctgta catggtattt attgaggacg tggaagacaa agacggcgag cgctgcatta
     8041 tgccaaccag cgagcatctg gaagacatgg tggtgctcgc ctggcagacc ggggcgtatg
     8101 ttttctgttt tgtcgaccgg gcccagcatc tggcgatgat gaaatccatc gggatgtatg
     8161 acgagcggct caacagcgac gaaaaagcca cgtacttttt caccggtgaa aatccgcaat
     8221 gggaacggga caaacccaca ggaatgaagc tgtgtgatta cctctatcga aaggtggagc
     8281 ggttcgccat cgagaacggt gtgcaggtgg ttgatgccgg gaagtgctca ccggagcaga
     8341 ttaacgcgat gattgaggtg gtcagcgagc ggcacgggga aagcgcggtg aacgagaagc
     8401 tgaaagcgga gctgatgcgg gaatataaca gccgctattt taacgtgccg gtgcctgaac
     8461 tgccggagga caaacccgcc cgccggtggt ggccgttcgg caaaaaataa cgccagcacc
     8521 ggcatggccg gtgctgttat tcttccccga gcttatcccg catcacctgc cagagtgttt
     8581 ccgcatcctc gtgaagccgt tcgcacatgt cggccggggt atccatgtcc agctcgttca
     8641 gcttttccag caacagcagc gactgatcct taatgaattt agccatattg agggcagttt
     8701 tttcctgctt cgtcatgttc tccattccct tcggtaagtt ctcagctaaa ccagccggac
     8761 cagaagcgtt ttttcttctg tgtcagttct gttgtcaatc tgtccacttc ggcctggagt
     8821 ttccggcgct catcatcgtg ctggcggcgt tcttcttccc gggatgtttt atcttccagc
     8881 atcagggtga cagcctgccg gagatcggcc agttcctttt gcattgccag aagctgttcc
     8941 tgtgggaaat ttttttgtgt attttcacgg tgtgaaatgc cgggaatttg tttctcaggg
     9001 atattcacat ttccgtatac acggatcagt tctgacacat ccacgacagg gttgtttttc
     9061 ccgtcacgtg acacggtaac ctttccctgt tttacatgat tgtatagcgt tcttctggta
     9121 atgcccgcgg cttgtgctgc ctgtgaaagg ttgagcaatg ttttcgccac tgtatacccc
     9181 tgattttatc gggtgtgaaa ggtgtgtaat ttctcacggt aaatgtatcg ctgaatttta
     9241 cccgtttttc cgttcaggat gtggttttct gacgcgctgc gcttgtcaaa ccggcgacgg
     9301 ccacgcaatg aattgcgccc ccgtcatcgg ttattagagg ctgtctgccc ccggataccc
     9361 ggattctttc tgatgcctga cggtgacgat cacaatctgg tccatttccc ggtcgtggcg
     9421 gtagagcatc acatagccac tgtcgccaaa cccgatcacc agttccttat attccagcgg
     9481 cagaaacggc accgggcggc caatgtctgg caatgttttc agttgctgga tagcccggac
     9541 gatcacctca cctccttttc tggctgccag ccgatttttg gttttgagaa agtcctgaag
     9601 gcgttgtaaa tcgcgctgtg ccagcgcgga aatcgttacc tgtggcatgg cggagcatcc
     9661 tgctcgttct cagttcccca ggtacttatc caggcttccg cttcttccgc agtaaggtgc
     9721 agacccgttt cctgatagtg ctgccatgcg gcctgtccgt cccgcaggta ctggctgcgc
     9781 ttctcttccc ggtcgatgta ttcggtgatt gcctccagca ttagcgcgtg cgctgagcgg
     9841 tgccggtcat cggccagcgt tttcagccgg tccttaagtt cttcatcgag cctgatggat
     9901 gttgctgatg acataaaagc ccctttgtag tcatatgtat tacacatgac tactttaatc
     9961 cgtggcggcg ctatttgcca gaaatacggc ttttgctgcc catttttcag cttcgcttcc
    10021 actaatcagg gctgctacag gctcactcat catccaggac
//
