LOCUS       NZ_CP066522             5596 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WCHKP090329 plasmid p3_090329, complete
            sequence.
ACCESSION   NZ_CP066522
VERSION     NZ_CP066522.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15649146
            Assembly: GCF_014041845.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 090329
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Wei,L., Wen,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 02:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,906
            CDSs (total)                      :: 5,785
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 57 of 170
            Pseudo Genes (incomplete)         :: 119 of 170
            Pseudo Genes (internal stop)      :: 20 of 170
            Pseudo Genes (multiple problems)  :: 24 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066522.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP090329"
                     /isolation_source="oral swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3_090329"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-06-18"
     gene            join(5215..5596,1..128)
                     /locus_tag="H3G99_RS29650"
     CDS             join(5215..5596,1..128)
                     /locus_tag="H3G99_RS29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            135..347
                     /locus_tag="H3G99_RS29255"
                     /old_locus_tag="H3G99_029255"
     CDS             135..347
                     /locus_tag="H3G99_RS29255"
                     /old_locus_tag="H3G99_029255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(723..851)
                     /locus_tag="H3G99_RS29770"
     CDS             complement(723..851)
                     /locus_tag="H3G99_RS29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1014..1238
                     /locus_tag="H3G99_RS29260"
                     /old_locus_tag="H3G99_029260"
     CDS             1014..1238
                     /locus_tag="H3G99_RS29260"
                     /old_locus_tag="H3G99_029260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1222..1488
                     /locus_tag="H3G99_RS29265"
                     /old_locus_tag="H3G99_029265"
     CDS             1222..1488
                     /locus_tag="H3G99_RS29265"
                     /old_locus_tag="H3G99_029265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1558..1908)
                     /locus_tag="H3G99_RS29270"
                     /old_locus_tag="H3G99_029270"
     CDS             complement(1558..1908)
                     /locus_tag="H3G99_RS29270"
                     /old_locus_tag="H3G99_029270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1974..2519)
                     /locus_tag="H3G99_RS29275"
                     /old_locus_tag="H3G99_029275"
     CDS             complement(1974..2519)
                     /locus_tag="H3G99_RS29275"
                     /old_locus_tag="H3G99_029275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2545..2871)
                     /locus_tag="H3G99_RS29280"
                     /old_locus_tag="H3G99_029280"
     CDS             complement(2545..2871)
                     /locus_tag="H3G99_RS29280"
                     /old_locus_tag="H3G99_029280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3229..3387)
                     /locus_tag="H3G99_RS29855"
     CDS             complement(3229..3387)
                     /locus_tag="H3G99_RS29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4027..4203
                     /locus_tag="H3G99_RS29285"
                     /old_locus_tag="H3G99_029285"
     CDS             4027..4203
                     /locus_tag="H3G99_RS29285"
                     /old_locus_tag="H3G99_029285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4193..4537
                     /locus_tag="H3G99_RS29290"
                     /old_locus_tag="H3G99_029290"
     CDS             4193..4537
                     /locus_tag="H3G99_RS29290"
                     /old_locus_tag="H3G99_029290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4746..>5180
                     /locus_tag="H3G99_RS29775"
                     /pseudo=""
     CDS             4746..>5180
                     /locus_tag="H3G99_RS29775"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3862..3941
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3279..3676)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..463
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.2300000000000003e-72"
     Typing          4528..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgtacctgga agacagcaac ggacgttttc tggtgctgcc gaaggggacg gccgtagaca
       61 ggacgcagag ctggacggtc gggaacgggc ggtcgaagca gaacgcgctg agactggtga
      121 aggagtaagt gccgatgacc gagctggaaa aacagttgct gagcgcatta gagcagctac
      181 agcaggacta ctcgaaaagg ctggacgagt gggagagcgc cttcggggaa tggcggaaaa
      241 tgtcagggct tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgacg cgcttgagtc
      301 agcaggtgga acgcttgagc gggcaactgc agcgtttgag ccggtagtgc agcgtcatga
      361 gatggccgtg gccgcagaac gggcgcacga gcaacggcag cacgaaaagg aactgacgga
      421 agcgcgttcc cggcagcgaa gctatgacgg tccgtcgctg ggctgaaatg caggaaaaca
      481 gggttctcat cgggcattta cgctgaaacc ggatgtcatt cacctggctg catggctatg
      541 cagccaggta aaatttatct gtcgcgaacg gggcggttta tcgggtggtt tgttgccggt
      601 tttacctgac tgccaccccg ttcgcggcga acccgtccgg ggcggtgcgg gcaacgggtg
      661 ttatgtttaa tcagcgtgaa cggcagatac aaaaaagccc cggagggctt ttaactactg
      721 acttattttt ttgaagattt gctgggtttc aatttgtcag tatttcccgg cgttttttta
      781 ttgtcacgct ggcgtttatc aggttcccct gttgaatcgg caattcgttt cggacccggc
      841 ttaagaccca taatcgttat cctttcaata gttaagagga aagccctcaa gctagatata
      901 ttgaccgcgt tacggattat caagaggggc taacaagatt attttttgtt cttttaggtt
      961 gattgtggta ctgttgatat acatgtatag caaatggtgt tcgggaggtt tatatgcttg
     1021 ctatccggtt atctgatgag attgagtccc gtctggactc gctggcgaag caaaccggca
     1081 gaacaaagac gttttatgcg cgggaagcaa tactggcgca tctggaagac ctggaggatt
     1141 attatctttc agcagaaact gctgcacgcg ttcgccgtgg tgatgaagca gtgcattcgt
     1201 ctgaagacgt gaggaagtca cttggtctgg acgattaact attccgatcg ggcgctcaaa
     1261 tcgttacgca agatggacaa acagaacgca cgacggattg tggattttat gagtttacgc
     1321 attgcagttg ctgccgatcc tcgccagtca gggaagccgc tcaaaggtga gctgggcgag
     1381 ttctggcgct atcgggtggg agattatcgc gttctgtgtg agatccgaga tgacgagctt
     1441 gttatccttg ccgccacgat tggacatcgc cgcgaagttt acgactgaag cccgctatgc
     1501 gggctttttt gatgccgaca acaggtatta tggtaaatac tcgactaact acgtgttcta
     1561 ctgtttgtcg gttttagtta accagcaggt tgtcttaacg ataatgtagc cgttattttt
     1621 aactaataag gtcatattgt taacgtcagg cataataaaa gacattatgg cctcagagtt
     1681 atcatcagaa gcagctctct gcattactac ttcgattccc ttttctttga atctataagt
     1741 atccagttgc ctattaaact tagaaagacc tacaaccata cttagtttgt tatctttaac
     1801 agtaagagat gcttcctgag taccattaca tcggtagtaa gcggtgtaac cgtcagcaaa
     1861 agatcgaccg gggaatataa atgctaggaa cgcaattaac caatacatag ttgagaatct
     1921 tggtattaca catttcacta tcctctcttt catttttcgt tcaccatact ttgttatctg
     1981 atttttaatt tttctatttt ataaaccaag tctctaccaa aaaggcgaaa attatcctca
     2041 aataataata attgtttaag aacatccatg aaagcagctg cttcaccctc agttatggga
     2101 gctactctat ataacatata acctgagtta agggtttctt ttatactttc ttcaatgctc
     2161 attataaata ttagtggatt atcatttttc ttcccccatg ggtttctaat taatccttcg
     2221 atgtactcag gtcctttgcg tcctattgcc tgcgcaatat tttttatttc atctgcgtga
     2281 gggctttgtt gtacagcttt gtttatgcct gcttgagtga ttttcagaga ctctacaccg
     2341 tgtatgttgt atgagataaa atgtaataaa ttaagatgca atgcaattaa tccatttttg
     2401 tttacatctg agtatttgga taaaaaacgt aatgaagtgt taactctccc ttgaagccta
     2461 acaattggga gtgctgcctt gtatatttcc ttctccgtta aataccgttt aaaaaacata
     2521 attctccgcc tccgtatagc gtgcctattt tatttcgtcg taaaacttag ctctgaaata
     2581 ttcctttaga accatcatgt cattaataat tgaatctatc tcatccattt tttcttggta
     2641 ttcttttaat ggtgaaggga gcggtaactc aatatttgaa aagtgattgg cgagataggg
     2701 atcaaggttt ctcatccatt caggcttttc gccaatgcgt tcttcaactg aaagtgaacc
     2761 tgataatctc tggttaataa gatcacttag actcattttg gttttattcg aaagccgttg
     2821 taaggattgc tttaatccta tatctagcat gatatttgtt ctttctctca ttaccccccc
     2881 ttgctattgc gttttttgtt gcgcacaaag ctaccattgc gcattaagaa acgcaataaa
     2941 aaaggaggga gggagtggtt tgtacatgat gtgcagtgcg ctgagggcat cagtggcggg
     3001 tgtttttccg tgggatggat ggagctccgg ggataacaga actgacgatc atttaaagga
     3061 tcttcttgag atcctttttt tctgcgcgta atctcttgca ctgtaaacga aaaaaccgcc
     3121 tggggaggcg gtttgatcga aggttaagtc agttggggaa ctgcttaacc gggtaactgg
     3181 gtatacgaga ccgcagatac caaaactgtt ctttcagtgt agccgcagtt aggcacccac
     3241 ttcaagacct ctcaatatct ctctcgtata tatccagtta ccaatggctg ctgccagtgg
     3301 cgtttttgcg tgtccgtccg ggttggactc aagatgatag ttaccggaaa gggcgcagca
     3361 gtcgggctga acggggggtt cgtgcataca gcccagcttg gagcgaacga cctaaaccga
     3421 gccgagatac cagcagcgtg agctatgaga aagcgccacg cttcccgaaa gggaaaaagg
     3481 cggaacaggt atccggtaaa cggcagggtc ggaacaggag agcgcacgag ggagccgccg
     3541 gagagaaatc accggcatct ttaagtcctg tcgggtttcg tcctcctctg acttgagcgt
     3601 ccatttctgt gatgctcgtc aggggaggcg gagcctatgg aaaaacggca acgccgcatc
     3661 cttttctccg gccctttctg atttgagcgc ctttgcttta tcccgttatc cgtgagtcgg
     3721 tcacgcccgc cgcagccgaa cggcaggagc gcagctaccg agtgagcgag gaggcgtcat
     3781 ttcatctgtc cgtgcatggt attgcggacg ctcctgagcc acatatcaca cctgccacat
     3841 gaggcacttt catgaatcgt gctctcatgc caacatagta agccagtata cacagcgtaa
     3901 acgctgtgac tggttcaggg ctgcgccccg aaacccgcta acaccactga cgcgctggcg
     3961 cttgtccaac gccggagcga cccggaaaac ttttccgtgc cgtccggcgt ttgcgaagga
     4021 gggataatgc agaagccgaa gaagaagccg ctcgtcgtaa aaaaaagatc ctggcatccg
     4081 gcagtgatgg caaatctgat gcgtcaggca caggaggaag attaccgccg ggagcagatg
     4141 gcagagcttg agcgttcagc ccggtcacat tttgcggata tcggaggaga ggatggcggt
     4201 taagcgtacc cgttttttag gtatccgggt gaccgatggg gagtaccagc agctgctgga
     4261 gcgctgcaat ggaaggcaac tggcggtgtg gatgcgggaa acctgtctcg atacgcgccc
     4321 ggcgcggtcg ttgcggctgc cgtccatcga tccggttttg ctgcgccagc ttgccgggat
     4381 ggggaacaac ctcaaccaga tcgcccggaa aattaacggc ggccagtggt caggcgctga
     4441 ccgggttcag gtggtggccg cgctgatggc cattgatgcc gaactcgagc ggctgcggca
     4501 tgccgtgctg gaaaaggggg cagacgatga tcgttaagtt tcatccgcga gggcgaggcg
     4561 gcggtgccgg gccggtggat tatctgctgg ggaaagaccg ccagcgcgac ggggccatcg
     4621 tcctgcaggg aaagccggag gaggtccggg agcttatcga cgcctcgccc tacgccaaaa
     4681 agtacacctc cggcgtcctg tcttttgctg aacaggattt accgcccggc cagcgtgaaa
     4741 agctgatggc gagcttcgag cgggttctga tgcccggact cgataaagac cagtacagcg
     4801 tgctgtgggt tgaacaccgg gacaaggggc ggctggaact gaattttctg gtaccgaaca
     4861 cggaactgct gaccggcagg cgtctccagc cgtactacga ccgggcagac cgtccgcgca
     4921 tcgatgcgtg gcagaccgtg gtgaacgggc gactggggct gcacgacccg aacgcaccgg
     4981 agaaccggcg cgcgctggtg acgccatctt cgttacccaa aacgaagcag gaagccgcag
     5041 aggctattac gcggggctta ctggccctcg cctcgtcagg ggagcttaaa acgcgtcagg
     5101 acgtcactga ggcgctggaa agcgcaggtt ttgaggtcgt gcgcaccaca aagagcagca
     5161 tcagcattgc cgacccggac ggagggcgaa acatccgact taagggagcc atctatgaac
     5221 agtcttttaa cgctggcgaa ggacttagag cagaaatcga aagcgcagca gcagagtacc
     5281 ggcgaaatgc tgaaagccgc attcagcgag cacgagaagt ctgtcagagc ggaactgagc
     5341 gaaagcggga agagaatcag cgccgccatc cgcgcccacg agcagggtat gagcgcggcc
     5401 atgcagtcga accgcctgag cgtgatgcgt atggtcgggc ggacatggct gaccatcacg
     5461 atggtgtcag gactgctgtt cgccagcctg agcggggtgt tgtggtatca ggggagcttg
     5521 atagcgtcaa atctggccga aatcgaccgg cagaacgcag cgctgtcgaa gctgaacgcg
     5581 aagacctggg gcgtga
//
