LOCUS       NZ_CP066521            11970 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WCHKP090329 plasmid p2_090329, complete
            sequence.
ACCESSION   NZ_CP066521
VERSION     NZ_CP066521.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15649146
            Assembly: GCF_014041845.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 090329
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Wei,L., Wen,H., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Wei,L., Wen,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 02:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,906
            CDSs (total)                      :: 5,785
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 57 of 170
            Pseudo Genes (incomplete)         :: 119 of 170
            Pseudo Genes (internal stop)      :: 20 of 170
            Pseudo Genes (multiple problems)  :: 24 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066521.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP090329"
                     /isolation_source="oral swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_090329"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-06-18"
     gene            complement(join(10717..11970,1..234))
                     /gene="ltrA"
                     /locus_tag="H3G99_RS29180"
                     /old_locus_tag="H3G99_029180"
     CDS             complement(join(10717..11970,1..234))
                     /gene="ltrA"
                     /locus_tag="H3G99_RS29180"
                     /old_locus_tag="H3G99_029180"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            879..1124
                     /locus_tag="H3G99_RS29185"
                     /old_locus_tag="H3G99_029185"
     CDS             879..1124
                     /locus_tag="H3G99_RS29185"
                     /old_locus_tag="H3G99_029185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1398..1769
                     /gene="tolA"
                     /locus_tag="H3G99_RS29190"
                     /old_locus_tag="H3G99_029190"
     CDS             1398..1769
                     /gene="tolA"
                     /locus_tag="H3G99_RS29190"
                     /old_locus_tag="H3G99_029190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1766..2131
                     /locus_tag="H3G99_RS29645"
     CDS             1766..2131
                     /locus_tag="H3G99_RS29645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            2306..2584
                     /locus_tag="H3G99_RS29200"
                     /old_locus_tag="H3G99_029200"
     CDS             2306..2584
                     /locus_tag="H3G99_RS29200"
                     /old_locus_tag="H3G99_029200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2572..2886
                     /locus_tag="H3G99_RS29205"
                     /old_locus_tag="H3G99_029205"
     CDS             2572..2886
                     /locus_tag="H3G99_RS29205"
                     /old_locus_tag="H3G99_029205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3050..3478
                     /locus_tag="H3G99_RS29210"
                     /old_locus_tag="H3G99_029210"
     CDS             3050..3478
                     /locus_tag="H3G99_RS29210"
                     /old_locus_tag="H3G99_029210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            3504..3683
                     /locus_tag="H3G99_RS29215"
                     /old_locus_tag="H3G99_029215"
     CDS             3504..3683
                     /locus_tag="H3G99_RS29215"
                     /old_locus_tag="H3G99_029215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3710..4240)
                     /locus_tag="H3G99_RS29220"
                     /old_locus_tag="H3G99_029220"
     CDS             complement(3710..4240)
                     /locus_tag="H3G99_RS29220"
                     /old_locus_tag="H3G99_029220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4247..4978)
                     /gene="mobC"
                     /locus_tag="H3G99_RS29225"
                     /old_locus_tag="H3G99_029225"
     CDS             complement(4247..4978)
                     /gene="mobC"
                     /locus_tag="H3G99_RS29225"
                     /old_locus_tag="H3G99_029225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4978..6942)
                     /locus_tag="H3G99_RS29230"
                     /old_locus_tag="H3G99_029230"
     CDS             complement(4978..6942)
                     /locus_tag="H3G99_RS29230"
                     /old_locus_tag="H3G99_029230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8426..8575)
                     /locus_tag="H3G99_RS29235"
                     /old_locus_tag="H3G99_029235"
     CDS             complement(8426..8575)
                     /locus_tag="H3G99_RS29235"
                     /old_locus_tag="H3G99_029235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8660..8917)
                     /locus_tag="H3G99_RS29240"
                     /old_locus_tag="H3G99_029240"
     CDS             complement(8660..8917)
                     /locus_tag="H3G99_RS29240"
                     /old_locus_tag="H3G99_029240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8927..10612)
                     /locus_tag="H3G99_RS29245"
                     /old_locus_tag="H3G99_029245"
     CDS             complement(8927..10612)
                     /locus_tag="H3G99_RS29245"
                     /old_locus_tag="H3G99_029245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3927..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7745..8323
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4251..4978)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tgtcagcagg tggaacaatg ctgtaaaccg ttctttcttc cgctgcttcg cagcttcccg
       61 cacgcgtgac agcctctgtg acatgctttc ccggctctgt gtccggcgca tgtgtggctg
      121 ttccgcgttc cccttggccc cgctccttcg ctccactgac tccgctcctt tcgggttgtt
      181 cgcctgcttc gccgctacta tgagcgagtc cgacttctcc tctccgtaca tcaccggcta
      241 tgactcctcg tcttcccggt gcgggccatc tccgacactg gcagatggtc agaggggaga
      301 tctcccggtt cccgcgtaga gatcgtattg acatgccagg gtctcagacc ccgccgggtc
      361 catgtggcac tcgcagtatc gcaccctatg atgttgcctt ccgttaacag tacaacgtcg
      421 gcacccggta atttaatata catttcgtgg ctcaatggct ggcctgtcaa cacccctgtc
      481 aacgcttcgc cccatacctc gcggtatgca acgcatgact cggggacctt gtggattgct
      541 ggtccttcaa tggtcgggga ctttcacccc ttgatctcta accggtctcc cggcgcacac
      601 tgtacatata cacagtataa ttattacctt actcctcccg ttttcaggtg tcaatatatg
      661 agattagttc tcatcttcag gcacaaaaaa gcgcccggag ccacctgtaa gcagatcgcg
      721 tcttttcccg aaaatttctc atggcaacaa attttattat ttaagacatt catcacactt
      781 acatttcaga tggtatagac acatctgaaa gctaactggc atgaggagag gacctgcggt
      841 taaactgttc gggaaacctg agacgcccgg aggccactat gacgcacctg aaactgactc
      901 ccgctgaaga aaagcgcctg gcggacgcct ttgtcgctca ggcactggcc atgggcctga
      961 aagggaacaa aacgctgcca aaggcggtaa aacaacaggt gcgggaaagg gtgcttcgtg
     1021 ccattcagga cgaacgtgaa gccaggcggg cgcaggaaat gcgtcgggag aaatacgagg
     1081 ctacggaaac caccttcacc tggcagccac agcgccgccg gtaaggggta tttaccataa
     1141 cgtcgcattg cccgcaccgc tcagacggtc tgagggcgtt tccggcggct acgggataaa
     1201 acaggcaaca aaccacccgg aaaaccgccg gaaacgcgcc cggtaaattg taacccgctg
     1261 catgcctatg cacgcaactg catggcgggc cgtttggtgt aaaaacgata cgataagccg
     1321 tgtttatgta aatgaacgtg gccgtaaaga gtgacaaata tacatgtaac attttttact
     1381 gacaggtcat caacccgatg cgtttaatac cctttatcct gctggcattg tgcagcccct
     1441 tactcctgac ggcctgtaag cattccacca cccagccgcc gaaaatttat actctggata
     1501 agtcatctaa aatcagtagc tacgctgcgg ccatcaagac cgctgttgaa atgcagatgt
     1561 ttgaaccaga ggcctatgcg ggaaaaatgt gcaccgtgcg gatatccata caacgtgatg
     1621 gcactctgaa cagcgccatt gctgaaggag gagatccgaa actctgtaaa gcagcaatat
     1681 cagccgttac ccacgccaaa attccgccgg caccggatga aaatacctgg caaatattca
     1741 aaaacgcccc tctggatttc agaccatgaa agcttttact ctgacagtgc tggcggccat
     1801 gttcctcact ccggtacatg cgataccact aaaacccgta accacactgc agccaaccta
     1861 cccggtacag gccgcagcaa aacgcatcga aggtcaggtg accgccacgt ttgatattct
     1921 ggacgatgga cgagtggaca acgtgtcgct taccggctgt cacctttttt ttcgggaaac
     1981 gctgacggca atgaggcact ggctatatcc cgcagggcac ccggcaatgc gacaggttat
     2041 aaattttgaa ttcagtctga aaaatgacgg cttacaggat ccgcgaccag tcaccaccca
     2101 aaagctcaat gatgatggtt gccgccagta acaggtcctt taacccgcag gctgcaatag
     2161 tcctggatga tgagtgagcc tgtagcagcc ctgattagtg gaagcgaagc tgaaaaatgg
     2221 gcagcaaaag ccgtatttct ggcaaatagc gccgccacgg attaaagtag tcatgtgtaa
     2281 tacatatgac tacaaagggg cttttatgtc atcagcaaca tccatcaggc tcgatgaaga
     2341 acttaaggac cggctgaaaa cgctggccga tgaccggcac cgctcagcgc acgcgctaat
     2401 gctggaggca atcaccgaat acatcgaccg ggaagagaag cgcagccagt acctgcggga
     2461 cggacaggcc gcatggcagc actatcagga aacgggtctg caccttactg cggaagaagc
     2521 ggaagcctgg ataagtacct ggggaactga gaacgagcag gatgctccgc catgccacag
     2581 gtaacgattt ccgcgctggc acagcgcgat ttacaacgcc ttcaggactt tctcaaaacc
     2641 aaaaatcggc tggcagccag aaaaggaggt gaggtgatcg tccgggctat ccagcaactg
     2701 aaaacattgc cagacattgg ccgcccggtg ccgtttctgc cgctggaata taaggaactg
     2761 gtgatcgggt ttggcgacag tggctatgtg atgctctacc gccacgaccg ggaaatggac
     2821 cagattgtga tcgtcaccgt caggcatcag aaagaatccg ggtatccggg ggcagacagc
     2881 ctctaataac cgatgacggg ggcgcaattc attgcgtggc cgtcgccggt ttgacaagcg
     2941 cagcgcgtca gaaaaccaca tcctgaacgg aaaaacgggt aaaattcagc gatacattta
     3001 ccgtgagaaa ttacacacct ttcacacccg ataaaatcag gggtatacag tggcgaaaac
     3061 attgctcaac ctttcacagg cagcacaagc cgcgggcatt accagaagaa cgctatacaa
     3121 tcatgtaaaa cagggaaagg ttaccgtgtc acgtgacggg aaaaacaacc ctgtcgtgga
     3181 tgtgtcagaa ctgatccgtg tatacggaaa tgtgaatatc cctgagaaac aaattcccgg
     3241 catttcacac cgtgaaaata cacaaaaaaa tttcccacag gaacagcttc tggcaatgca
     3301 aaaggaactg gccgatctcc ggcaggctgt caccctgatg ctggaagata aaacatcccg
     3361 ggaagaagaa cgccgccagc acgatgatga gcgccggaaa ctccaggccg aagtggacag
     3421 attgacaaca gaactgacac agaagaaaaa acgcttctgg tccggctggt ttagctgaga
     3481 acttaccgaa gggaatggag aacatgacga agcaggaaaa aactgccctc aatatggcta
     3541 aattcattaa ggatcagtcg ctgctgttgc tggaaaagct gaacgagctg gacatggata
     3601 ccccggccga catgtgcgaa cggcttcacg aggatgcgga aacactctgg caggtgatgc
     3661 gggataagct cggggaagaa taacagcacc ggccatgccg gtgctggcgt tattttttgc
     3721 cgaacggcca ccaccggcgg gcgggtttgt cctccggcag ttcaggcacc ggcacgttaa
     3781 aatagcggct gttatattcc cgcatcagct ccgctttcag cttctcgttc accgcgcttt
     3841 ccccgtgccg ctcgctgacc acctcaatca tcgcgttaat ctgctccggt gagcacttcc
     3901 cggcatcaac cacctgcaca ccgttctcga tggcgaaccg ctccaccttt cgatagaggt
     3961 aatcacacag cttcattcct gtgggtttgt cccgttccca ttgcggattt tcaccggtga
     4021 aaaagtacgt ggctttttcg tcgctgttga gccgctcgtc atacatcccg atggatttca
     4081 tcatcgccag atgctgggcc cggtcgacaa aacagaaaac atacgccccg gtctgccagg
     4141 cgagcaccac catgtcttcc agatgctcgc tggttggcat aatgcagcgc tcgccgtctt
     4201 tgtcttccac gtcctcaata aataccatgt acagcgccat aatccctcac tccgttgtca
     4261 taaatttaaa gcacgcgaac agcctctccc ggtccagctc caccgttttg cgggcctcgc
     4321 cggtctgtgg attcacgatt tcatccaccg ccagaatcgc ccgctgtacc cggacaagcg
     4381 tcggccggtc agggcagatg taccagattt catcgatgcc gttcgcccgg cggttgaaca
     4441 gatacccgga caccacttcc tgataccgcc tgcggctctt gacggtgcgc tctacctcaa
     4501 atgccaccgt tctgccgtcc agctccgcca gcgcgtccgg cactttcatc cctttcaggg
     4561 ccatacggtg caggtaccgc cagccggtgg ccccccggcc ctccatggag aggcgcaggg
     4621 actgcacggc cagccggtgc ggcagctgcg ccgccgacac ccttgacggc tgaaacggaa
     4681 taacgtccgt cagcggctcg tcctcgtcga acgagaacgc caccccgtgc ggcgtcagcc
     4741 cccacacgct gacctgacgg ccgtcaaccg ggtatttcgc ccggaccagc agccctttcg
     4801 ccaccagact gtccaggaag cggtaaaccg gctgtaccgc cgcaatcccc agccgtcgcg
     4861 caaccagctc cgccgtggaa cacagctctt cccgcagcca gcgcagggtc agccgtgttt
     4921 tctccgcgtt ccgcgcctgc cgcttcgcgt ggctgacgtt gtactgctcc agtgtcatca
     4981 gtaaagcccc gcaaaatcat catccgcttt tggtccgccg ccgtcggccg gaggcaggta
     5041 ctccgccagc gggtccgttt tcgccagcac cgggaaggtt ttcagcgaaa tttcccggcg
     5101 ctcaacccgc agcgggctgc tgaacgccag ccgcgccacg cccagcccgg tcatcaccgc
     5161 gaacccgtca ggcaggtgtt gcagggtgtt cacgtcaaac agcggccgcg cctccttctg
     5221 gatgaagctg tcgccgctga tgtgctccac gttccccgcc tcccggctgg cccggcggcg
     5281 ctccacgtcc acccggatct ccccggtctg gcccgccgcc cactgcgcgg actcctggct
     5341 ggccgcgcga tagaaccagc ggatcggcgt gttatccagt accgtggttt tcacaaaatc
     5401 agcgggcaga tcctgcccgc actgaccgaa gtcgcccagc gactggtgcg cgagcagcag
     5461 gttgcagtcc cggctgcgca gcgtccccag cgcgttcagc acgtacttcg acagcagata
     5521 tttaatctca tcgagcatga tgctcgcgtg cggccacctc cggaactcat cccgcgcaat
     5581 gatgatttgc gcgcagcggg caaacagcat cttctgcacc cggatcaccg cctcatcgtc
     5641 catcgagccg atgacgtaca ggcagccgcc gccgttgatt atccccgcca catcgatacc
     5701 gctgtccgtc tgcaggacgc tcaggtcggc cacgttctcc agcttggtga tcagcccctt
     5761 cacgtccttt ttcagggcct ccggcaacgc gtacgccgcc gccagcacct gctgaatgtt
     5821 tgccccctgc gggaactgct ccgcaatcag cttcgccgct ttctgctcgc tgatgcggta
     5881 gtggtccgcc acgtcgccgg tctcactcag gttaaacccg gcaaccagca gattcttcag
     5941 ggcgtactgg tcgagatcgc ggaacagatt cagctgcgcc ggtttccccg gccgcagatc
     6001 cagcagggtg aagtccgggc agtgcgcccg gaagacgtgc ggcgcccacg cgtccccttt
     6061 ggggtcgaag tacaccacag cgtccccgaa atcgcgtacg cagcgaatga gcgcgttggt
     6121 ggccatcgcg ctcttgccgc tcccgggcag gccgaggatc tgaatattgg tcttgcgcca
     6181 ggtatcacga gggaccagca ccggctggcc ctgctcatcc ttacccagaa acaccgcctg
     6241 ctgtttccgg gcctgctccg tggaaaaatc ggccagcgcg ttaaccggta ccggctcccc
     6301 gaaatgcagg tggcggacgt ctgtcatttc tccggtccgc tggctgctgc gccggctctc
     6361 ctgattcagt ttcgcgttca ccgcctcgtc agcggccgca aaatgaccat caagcagcag
     6421 gaacgccagc agccgcgcca gaaggacgcc ggcgacaagc cctccggcac accaggctat
     6481 cccgaacagg ttagccggct gcagcaggta ataatctgcg ttcgcataga ggtccgggcg
     6541 caggacggtt tctccccagc tctcgataaa cacggcgaca accagccccc ccagaagggc
     6601 cagaccggca gcgccggtca gccagtacgc gtagttccag acatgtcgcc gtgccctgac
     6661 catccgcccg ggtgaatcca gcgaggccag caccgaaaat ggcacagtcc ggttattatc
     6721 gcgcttatag cgatcggcat actcccgccg cgccttatgg ctcagcccca caaaacgcat
     6781 tcggccggcc cagagttcag ccagaccgaa aaacaggccg ataaccagaa aaaaaccggc
     6841 cagactgtaa tactcccggt acagccagct gacccacggg taaatctgcc gggccagctc
     6901 cgggatatgc cactgccagg caagcgaatc cgtattaaac atgcaatctc tctccttcag
     6961 acgacgggtt taccggcgtg atgcgcagcc cccgctgcac aagccgcgga catgccccaa
     7021 ggcatggcgg cggcggtcac gtccgtggtg attgcgttca gctttcaccg cggaggccag
     7081 cctctttccg ccccatgcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc
     7141 gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag
     7201 agttcgccat agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgtc
     7261 aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga cgggggccgg
     7321 gtgaatttct ggcgtggccg gcgtccagcc ggacagagcc agaaagaggg gaaaagaccg
     7381 tgcgtcggtg gcgtccagcc agcgtaagca aggtcttttc ccctgaagtc agccacgccc
     7441 cagcggggat gacttgcatt tctccgcaag gagaaaaaag caacagagat aggttaacgt
     7501 aaaatgttca gcgaaaaacc gacatggttc acctatcctg ataattgatc gtcagggaat
     7561 agaaagactt aatcagggga caatatccca catcagcgcg tccggtgacc gtcttgcagg
     7621 tgccggggaa attgcgcctc ctcgctcact cggtcgctac gctccgggcg tgagactgcg
     7681 gcgggcgctg cggacacata caaagttacc cacagattcc gtggataagc aggggactaa
     7741 catgtgaggc aaaacggcag ggccgcgccg gtggcgtttt tccataggct ccgccctcct
     7801 gccagagttc acataaacag acgctttttc ggtgcatctg tgggagccgt gaggctcaac
     7861 catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac cgtttccggt
     7921 aggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg tttaccggat acctgtccgc
     7981 cgttctccct tgcgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc
     8041 ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg
     8101 ctgcgcctta tccggtaact gttcacttga gtccaacccg ggaaagcacg caaaacgcca
     8161 ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt
     8221 tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc
     8281 tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa
     8341 ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga
     8401 tctcaagaag atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaggagg
     8461 acgatggtgc caccgttccg ccctgaacat cccggatata atttgcctga catgcggcca
     8521 gaagaaagcc agcaacaatc aatataaaaa gaaaaatagt ctttaccttt ttcatatact
     8581 ttccctgtat ttaaagactc cggcattcat ccctgaacac cggaagtcag tatttcaaat
     8641 catccttgat aattttttgt taccagttac cgtcccgata atcaaaagct atataacaat
     8701 tatccacatc aggttttatt tgatttttaa aaaatggctg tagtaaagga acccattctt
     8761 tcttcaaatc aaactcacca ttattaatat tatcctttaa aggcattccc aagctttcaa
     8821 tgacagaacc atcgtcacca aagtctttag aatactcttc ccctttaaac tcttcagttt
     8881 tcttatcaaa ccatgtcaaa cgtaatttaa gccccatatt tacctctcaa agatattttt
     8941 taatgttccg ttttggatct ggccctttaa cctgcttacc cgttttggga tcaaatgcgc
     9001 ctaggtgttc gccatcactg gcacgatacc cttcaagctc accgtgctgg gaatcccatt
     9061 cataaatttt acgtttttta tcgccatacc atcgggcccg cttaccacca ccaccttgtt
     9121 taggtgtttt aggatcacct cgctttaact ctccaagtcc ctttatgtct tctgttttag
     9181 gtactggatg ataatcatgg ccgtagtctt tagtcccctt gcgaggcttg ttcttttcct
     9241 catttaactt attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg
     9301 ccccggtaag tgcagcctta tctctggata aggattcatt cattgcgttt aaagatgcct
     9361 gagccgcttt taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat
     9421 cctgccaccc ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg
     9481 cctctgcctc ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggagcg
     9541 ccttctctgt cgatgcaatg cggctgttta aggtcgcaat atttttatct tcggcatcca
     9601 gctcagcttt cgctttatca tagtctcttt tcagcccctc ttccggatga gcggcgtccc
     9661 atgcctgctg gcgacgcttt tcttcctcct gacgctgttt tacctgtgcc ggggtaagaa
     9721 catctgtcac cgacacataa accggcttct gtccggtctc tttcgggaaa cgaataacgg
     9781 cctcacggga gttaccaccg gctgtaaaac cagccggacg tgaatcacct ttttcagcaa
     9841 caatgccttt tggcggggct ttcgctgtcg gaacaccttt cggtacgctc acctgcagag
     9901 acgggagacc cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca
     9961 ccacagggac actcattggc cgacctgcga caaccgcaat atgctgccgc tcatccttca
    10021 ccacatccac aacccgttgt ctgacacgaa ccgtcgcctg gtccagtggt aaagtacttg
    10081 ccggagtctc cgtcaccgca tcggccggta atgacgtcat aatttttgac atcattttcg
    10141 gatcatcttt tgcaatctca gaaggcagca caccgtagat cgcaatcccc cacagaccaa
    10201 acttaaacgg ccccttcagg gcagccagca catcagcaac ggcggccgac aacgcatcgc
    10261 cggaaactga taaagccagt ccttcagccc ccggagtagc cagggcaggc agaccgaagg
    10321 ccatcgcagt ggccgaagac tgtccgccac cagagctacc gctatgattg ccgctgttac
    10381 caccattacc accgggttta ctcccgccgt taccaaactc ccccggatta tagttatgaa
    10441 tatctccatt cggcgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac
    10501 cacctgatga agggccacct ttaccagacg taccgttcac attcccactg gcctgaccac
    10561 cattatgtcc cagacctgaa ttacccggac ctcgaccgtc tccaccactc ataccgtttt
    10621 cctctttgac atttaaaaca ataaattaaa gccaacatgt cgcggtaggg atacccgtta
    10681 ccggataccc cccgcacaga tcccggcgtg cgcgatttac gcaccgggct cctgcctcgg
    10741 gtgtctggcg gtgaaccgct ccacaggcca tggatgaaga acccgaaccc ttggtagcca
    10801 tgcggctgcc agtttgtttg ctttcgtcca ggtcgtatca tccttctggc tcctgcgcct
    10861 gagcgcccgg cgccagaggt ttgttacgtg tgtcctgaac ttctgcatgg tggggaagtt
    10921 gcccggtacc gagtgatagt tcaggtatcc ctgaaccact ctcctgagcc attttccctg
    10981 ttcggggatt gagtaatgcc agcgccttcg cagaccgtct ttgatggctt tcagagttgc
    11041 cgtcatccga tcccggcggg tctttcgtat cagcatgaac ctgccgttgc gatctttccc
    11101 gctgatgtgc gtgaacccga ggaagttgaa cgtttctggt ttgccttttc ccctgatggc
    11161 acggttttcg gcagcgaagc ggccgaactc catcagacgg gttttctccg ggtgaaccgt
    11221 gagtccgaac tccctcagtc tgcgctgcat ggctatacgg aagcgccggg catcgtatcg
    11281 tttgtcgaac ccgatgacga tgtcatcggc gtatctgacc attaccacat tgcctgtggc
    11341 atagcgacgt cgccactgat gcgcccacag atcgaagacg tagtggaggt atatgtttgc
    11401 cagcagcggt gagatgaccg caccctgtgg ggtgccttcc tccgttgctc gccattgacc
    11461 ctcctccgac gtcccggctg tgagccactt acgtatgagc ctgattaccc tccggtcgcc
    11521 gatccgatgc tctgtgaacc tgatcagcca ttcgtggctc accctgtcga agaactgact
    11581 gatgtcggca tccagtaccc agtttacgtt agtgcgtacc agccctgtgg ccagtgcgtc
    11641 cagtgcatcg tgctggcttc gcccgggtct gaacccgtat gagaacccca taaagtcgtt
    11701 ttcatagact gcgttcagga ttttcaccag cgcatactgg acgatcttgt cctccagcga
    11761 ggcgatgccg agcgggcgtt gttttccatc cgcttttggg atgtagtgac gcctgccggg
    11821 ctgcgccctg tagctgccct gatgtagcct ccggtgcaga tctgttatgt tgttcttcat
    11881 gtttccggcg tagtccatcc acctgatgcc atccactccg gcggccgctt tcctgctcag
    11941 ggagaggaat gcggcttcca gtgcttcgac
//
