LOCUS       NZ_CP066469            10968 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 91 plasmid p91_10kb, complete
            sequence.
ACCESSION   NZ_CP066469
VERSION     NZ_CP066469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038086
            Assembly: GCF_016406345.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 210.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/19/2024 04:00:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 41 of 112
            Pseudo Genes (incomplete)         :: 65 of 112
            Pseudo Genes (internal stop)      :: 30 of 112
            Pseudo Genes (multiple problems)  :: 20 of 112
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066469.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 91"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p91_10kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            1..1041
                     /locus_tag="JCQ04_RS15330"
                     /old_locus_tag="JCQ04_15330"
     CDS             1..1041
                     /locus_tag="JCQ04_RS15330"
                     /old_locus_tag="JCQ04_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_000997689.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLLK
                     DRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVN
                     KRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIA
                     KIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKE
                     DSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDA
                     DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVS
                     TENWLE"
                     /name="replication initiator protein A"
     gene            complement(1657..2337)
                     /locus_tag="JCQ04_RS15335"
                     /old_locus_tag="JCQ04_15335"
     CDS             complement(1657..2337)
                     /locus_tag="JCQ04_RS15335"
                     /old_locus_tag="JCQ04_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_002287920.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIGADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <2413..2979
                     /locus_tag="JCQ04_RS15340"
                     /old_locus_tag="JCQ04_15340"
                     /pseudo=""
     CDS             <2413..2979
                     /locus_tag="JCQ04_RS15340"
                     /old_locus_tag="JCQ04_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3026..3262)
                     /locus_tag="JCQ04_RS15815"
     CDS             complement(3026..3262)
                     /locus_tag="JCQ04_RS15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306297428.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISFF
                     LLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
                     /name="hypothetical protein"
     gene            3360..3539
                     /locus_tag="JCQ04_RS15345"
                     /old_locus_tag="JCQ04_15345"
     CDS             3360..3539
                     /locus_tag="JCQ04_RS15345"
                     /old_locus_tag="JCQ04_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001120991.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLLI
                     AQEKAGFEVPALKK"
                     /name="hypothetical protein"
     gene            3670..3939
                     /locus_tag="JCQ04_RS15350"
                     /old_locus_tag="JCQ04_15350"
     CDS             3670..3939
                     /locus_tag="JCQ04_RS15350"
                     /old_locus_tag="JCQ04_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_000388479.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRD
                     YDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            3932..4189
                     /locus_tag="JCQ04_RS15355"
                     /old_locus_tag="JCQ04_15355"
     CDS             3932..4189
                     /locus_tag="JCQ04_RS15355"
                     /old_locus_tag="JCQ04_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_000588503.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGK
                     PEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            4648..5652
                     /locus_tag="JCQ04_RS15360"
                     /old_locus_tag="JCQ04_15360"
     CDS             4648..5652
                     /locus_tag="JCQ04_RS15360"
                     /old_locus_tag="JCQ04_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455809.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINVF
                     FYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACEI
                     GVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKL
                     VHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQITL
                     SKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
                     /name="hypothetical protein"
     gene            complement(5817..6431)
                     /locus_tag="JCQ04_RS15365"
                     /old_locus_tag="JCQ04_15365"
     CDS             complement(5817..6431)
                     /locus_tag="JCQ04_RS15365"
                     /old_locus_tag="JCQ04_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001261742.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRPV
                     FQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPL
                     LDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRL
                     VYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
                     /name="recombinase family protein"
     gene            6881..8206
                     /locus_tag="JCQ04_RS15370"
                     /old_locus_tag="JCQ04_15370"
     CDS             6881..8206
                     /locus_tag="JCQ04_RS15370"
                     /old_locus_tag="JCQ04_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_000969590.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVG
                     VGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMG
                     IMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
                     /name="Y-family DNA polymerase"
     gene            8199..8549
                     /locus_tag="JCQ04_RS15375"
                     /old_locus_tag="JCQ04_15375"
     CDS             8199..8549
                     /locus_tag="JCQ04_RS15375"
                     /old_locus_tag="JCQ04_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000568378.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTEE
                     IAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIRHI
                     EFYQDNKWSRLE"
                     /name="hypothetical protein"
     gene            8546..8725
                     /locus_tag="JCQ04_RS15380"
                     /old_locus_tag="JCQ04_15380"
     CDS             8546..8725
                     /locus_tag="JCQ04_RS15380"
                     /old_locus_tag="JCQ04_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001021554.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHNFRFYIFPLYI"
                     /name="hypothetical protein"
     gene            8861..9151
                     /locus_tag="JCQ04_RS15385"
                     /old_locus_tag="JCQ04_15385"
     CDS             8861..9151
                     /locus_tag="JCQ04_RS15385"
                     /old_locus_tag="JCQ04_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000026576.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITRQ
                     RRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
                     /name="hypothetical protein"
     gene            9519..10307
                     /locus_tag="JCQ04_RS15390"
                     /old_locus_tag="JCQ04_15390"
     CDS             9519..10307
                     /locus_tag="JCQ04_RS15390"
                     /old_locus_tag="JCQ04_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000101106.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQGN
                     ATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVGSE
                     HRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKLSET
                     IETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRTITVD
                     EAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
                     /name="AAA family ATPase"
     gene            10294..10620
                     /locus_tag="JCQ04_RS15395"
                     /old_locus_tag="JCQ04_15395"
     CDS             10294..10620
                     /locus_tag="JCQ04_RS15395"
                     /old_locus_tag="JCQ04_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000796719.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKDQ
                     GSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLAKK
                     QNKI"
                     /name="hypothetical protein"
     Typing          2..1041
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgatt ttaattatta caaatcaaaa gaaatttatc gtgaaaaata ttatcaaatg
       61 cctaaggtat tttttactaa cgaaaaatac atggatctat caaatgatgc aaaaattgct
      121 tatatgctac ttaaagatcg atttgattat tcagtcaaaa ataactgggt agattctgat
      181 gataatatat ttttcatatt cacagttgaa gaattaatga aattgttaca atgccgcgaa
      241 ggtaaagtat ctaaaatcaa aaaggaatta gaaaaagcag gtcttctgaa acagaagaga
      301 ggaagagtaa ataaacgtga cggtaagatt gaatccatgc ctaatttatt gtacttaggc
      361 aagccagaag taacgaatca agacgtcttt aaaatcatgg aagaggaaga caatactgat
      421 accaccgtaa ttgcgaaaat cgcaaatacg gcaaaaccgc gagaaatcaa tgataccacc
      481 gtaattgcga aaatcgcaaa tacggcaaaa tccagtcata tcaaggactc aaccgtaatt
      541 gcgaaaatcg cagacaatct attctattct aattctatag acactaatag acacataata
      601 gacactgaaa ctgatcagtt acaaaatcaa gttttactag ataacttcgt cgacatcatg
      661 aaagaggaca gtataaatac atttgtacca gaaaatgtat taaacttgat aaaaacattt
      721 tctagtactt acgaagacgc tcaaaaaacc gtccaaacta ttcataatgc aaagaaaaaa
      781 gctgaagaat tagagcaaac agttgttgtt tatgaagaac tggaatctta tggaattgat
      841 gctgacaaag gattatatat gactttgctg aaagcttatc aaaaacaaaa aactgaaaaa
      901 gtaaacaatt tgcagaatct aattttcgtt tatgtaaaaa attggtttgt cgaaaaagct
      961 attgcggcaa agcttgcaaa cgaacaaaga gaaacattta acgaattacc aacagttagt
     1021 acggaaaatt ggctagagta gttgataaaa agcgatcact ataaaaatga tcaattttaa
     1081 attagaactt ttttatgatt ttaattttat tacaaatgtt acaaataaat agctattatt
     1141 ccggagtttt tagattaaaa gtttatgaaa acaatcacaa acgtaggttt taaagtcata
     1201 atcgttagat attttagtta tttttcattc aaaaaaatag ggaactagtg tttgcatatg
     1261 tgcttttttg agttataata attcatgaag agaggtaatt cacttatttc cccaagctga
     1321 aaagcctgat attacttctt ttcaggcagt gctgctaata ttcaattaca cttttttgta
     1381 tatttttagt tgttttaaat ttaattgtct gatatgcttg tgatgaattg attttcgttc
     1441 tgcacttttg gacgataaat tgtgataatt gaaaatacaa aaaaaagaaa ggcccctgta
     1501 aggctcggga gactggtaat ctccgtcagt gtcctacagg aaagcattaa gatgacgcct
     1561 taatgcgtgc ccttcagtgt tacggtatta cccgcaacgg acggttctgt tgcaaagttt
     1621 taaataaaga ataaaatccc ttacggtatc tatgatttaa gctgggattc ccaataatac
     1681 cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg ttttcttata
     1741 taatcctcga atggcttcca tgcctttaat cgtggtagag gcagtgcgta aacttcgata
     1801 gaatttattg cgtctcttta ctggacgatg gtcttgttca atcaaattat tcaggtattt
     1861 aatggtacga tgttctgtcc cttgataaaa gccgtattct tttagtttct taaaggcact
     1921 tgtaatagag ggggctttat ctgtgactac aaccttcggt tcatcaaact gcttcactaa
     1981 ccgcttaaga aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa
     2041 ggttaaacca tctgcaccga tggctcgata caaataatgc cattttcctt taattttgat
     2101 gtacgtttca tccattttcc atgaataaaa ggatttttta tttttctttt tccaaatttg
     2161 atagagtagt ttgccatatt cttgcaccca acgataaatc gtcgtatgag aaacgttaat
     2221 gccacgatca tataagattt cttgaacttc acgatagcta aggttataac gaagatagta
     2281 gcccacggct acaataatca catcctgctg aaattgcttt cctttaaaat gattcatcgt
     2341 cattcctcct gctatctttt tctattattc taccttattt gatagtagat ttaaaacttt
     2401 gcaacagaac ccgaagaaca attgtatttg gaaagtgaaa cgagtcaaac gaaggtcaca
     2461 gtcagccata atcgtttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaacg
     2521 gagcttctac gactagaaac agaaaaatac atcatgaatg tttacctaga aaggagtgag
     2581 ctggtcgaaa actttgtcag ctacacgatt tgcaggaaac actattactc agaatcagaa
     2641 tattattcaa tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc
     2701 aaagatgcga cgatagacga ttattacctt gtatttacta gatttttgac gtgtggaacg
     2761 gaatctatcg tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg
     2821 gaatatgtag aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt
     2881 ttagacacct atacttacga cgatacagaa gaaatccaaa atgaacttga ccgtaaacga
     2941 atgccatatc aacgatttga aaggaaaaaa aggcaataat catttatacc gaaaagtgag
     3001 aatgtgtgaa ataaggacaa aaaacctagt aactaaggga atggttctta tttcgcacca
     3061 atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata agcgggcaac acccgataac
     3121 ctaagagaaa gaaggagatc catatgaata aaatcattcc tctattgtta gtcctatttg
     3181 ttggcgaaat acttatgcgc catgctgcag ctgtgcattt aaacttcgta gtatttttgg
     3241 caatactggt attttgtttg atcttttggc gctttgtatt agcccttgtg agtctactat
     3301 tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa
     3361 tgccaattgc aataggaaat aaacggttac ccgttacgtt ggatgaaaag agacaaaaag
     3421 aattgcagca actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt
     3481 tagatttatt gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac
     3541 gtcacttttt atcctaaaaa ctaaaagtga tagcactttt aattataaga agttagaata
     3601 ttaatcattt gcttaattgt acaatataat gtacaattgt tttatagaaa taaataaggg
     3661 gtgaaaggaa tggaagcagt agcttattca aatttccgcc aaaatttacg tagttatatg
     3721 aaacaagtta atgaggatgc tgaaacactt attgtaacaa gtaaagatgt agaagataca
     3781 gttgttgtat tatcaaaaag agattatgat tctatgcaag aaacgttgag aacactttct
     3841 aataattacg tcatggaaaa aattcgtcga ggagatgaac aattctccaa aggtgcattt
     3901 aaaacacatg acttaatcga ggttgaatct gatgattaag gcttggtctg atgatgcttg
     3961 ggatgattat ctttattggc atgagcaagg aaacaaaagc aatataaaaa agattaacaa
     4021 gttaataaaa gatatcgatc gttccccctt tgctggatta ggaaaacctg agccattaaa
     4081 gcatgattta tctggaaaat ggtccagaag aattacagat gaacatagac tgatatatag
     4141 agttgaaaat gaaacgatat ttatttattc tgcaaaagat cactattaac caatcggaag
     4201 taaggaaagg gtcagaaact taaaagtttt tgatccttat tttatttacc ctagtcattt
     4261 aaaaagctaa tatagcttag tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta
     4321 cggatataag gttagtaaaa tcatttctaa agttgaggaa aagtaaatat aaatggctta
     4381 aatttcaaca atttgaagtt gaatagatat gttataatac tattgtagtg tgggatgtta
     4441 gttactaaag gatgacgctt atatatatga ctgaatagaa taagcaatag gtttaataat
     4501 ctattttaaa ttttttgtac tagttttagt taattagcaa aaacaacaaa aataaacttc
     4561 tcatagaatt tagctaaaaa ttaatgattt atttacatat taaatttgga tacagttaag
     4621 taatttttat atattggagg agaagtaatg gaatataaat ttaacttgaa tttgaaagaa
     4681 gtactcccca ctcagggaac aataaacata aatggtcaag ataatgatga cgatatagta
     4741 ataaaaaaaa tagaatttat tgattctgat ataaatgttt ttttttattc taaagaaaaa
     4801 ttagctgatt ttagcaatat tgctgaaatg attgaacgtt taaaagattt tatgatcagt
     4861 gtttgggatg tagcgatgct tgctgacgaa tcaaattata cttcgagcat atacagatta
     4921 gaagatagta attggataga aatttcatta gagttaaaga cacttcctaa ttccgttaac
     4981 tatataatta gagatataac ttctgaagct caacaattag ttaattacat gcatggacac
     5041 gaaagtatcc ccttttctct tatactcctt aaaagaagta aaaatatatc aaatactaga
     5101 gataggttta tttcgataat tacagcctgt gaaatagggg taaaggaatt ttatcaacaa
     5161 gagaaacctg atttaactct aatatttgag aatttacaat ctccccctgt agacaaatta
     5221 ttagggagca tgtttaaagg gtactttaat attgagtttc cgaaagaaat aagaaaggaa
     5281 attaaaaatt tcatagaaaa acgtaataaa ttggttcatt ctcctcaagg aagtaaacca
     5341 actatagaag aatgttttga atgttatatg gcagtgctaa aaacatttaa ttttttttat
     5401 aaaattaatg atagctttat gtatagagat ttttatgaag aatctataga attgactcca
     5461 acggaaccaa ataaatctca aatcactttg tcaaaggagc tagagagaaa agtagagtct
     5521 caagaactag ctataggaat tagtataacc aatgtgtcga catacaaaga attatacgaa
     5581 gctaaatcac aagataataa ggattttgta gaaaatatag ataaggaacc aacaaataca
     5641 gtaggggatt aacgtaaata agtgaagatt atattaacag actctttatt tagttaagta
     5701 aaaaatggtt tgttggaatt ttatgcttta tctgttctga gtaagactta attttataga
     5761 tagtaattat atgtaggaaa ttaaagatga gaagataata aaagcagcat gatcaactat
     5821 ttgtcattga ccatgctgtc tttttttatt ctatacacgg tttgtctcgt aacattgtag
     5881 tctttagcaa tcttagcaat cgcaacacca tttttaagat cttcaacgat gcttttgtaa
     5941 acaaggcgac gttggggatc ttttgcgtct gcactgtata atttgggccg ccctttgtag
     6001 acgccgtttg cttttgcgat cttaattcct tgtgcctggc gacgtttcga ttcagtccgt
     6061 tcttgttcag cgatcatggc caaaatttgg atgatcagat ccttaatgaa tttatccagg
     6121 agaggattgc caattgcttc agccatgatc ggtaaactag taatgatcag cttcacattt
     6181 ttcttcttca agtaattgac tgaagcgatg atctcgtcat aatttcttcc aagtcgatca
     6241 atagcttcca caataaaaat atcttgatct cggacgtaat ctaatgcttc ttggaaaaca
     6301 ggtcgatgag taatcgttgc gccagattgt ttctcgatga aaattctctc tgcaccgaat
     6361 tttttcattt cttcaatctg gcgttgttca ttttgatcga tcgatgagac acgtatgtat
     6421 gcaactctca aaacttttac ctactttctg caactattta aaatacaccc taatcataca
     6481 cgaggaagcc atatttttca atgttatacc agtgtctatt tagggtaaac cttatataga
     6541 caaaaactaa tgatggagga aatagtatga gaattttttg taagtgtcta ctgataactt
     6601 attctcaaat gttgtaagga ttttattcat ttgttaatat taaagaacgg aactccaaaa
     6661 aagtccgtac tctaatttgc agtctctcac tcttttagag tgagagtttt tattagattt
     6721 tcaaattaat tagtaaagtg atagaataaa ttgatcatac aaatcaaaag aagaagcagg
     6781 gtgatagcat acctatgctt ctttttttat gtatttagct atttcttctt tacaatatcg
     6841 aacatatgtt tgtataatgt gaaagaatgg agtgttattt atgatggatt attcaaaaga
     6901 gccagtaaat gattactttt taatcgatat gaaaagcttt tacgcaagtg tcgaatgtat
     6961 agaaagaaat ttagatccat taacaacaga acttgttgtt atgagtcgag ctgataatac
     7021 tggttcagga ttgatattag cttcttctcc tgaagcaaaa aagcggtatg gtattacaaa
     7081 tgtgagtaga ccacgtgact taccacaacc atttcctaaa acactacacg ttgttccacc
     7141 acgtatgaac ctatatatca agcgaaatat gcaggtaaat aatattttca gaagatatgt
     7201 ggctgatgaa gatctactga tttactcgat cgatgaatca atcttaaaag tgacccgatc
     7261 actgaatctt tttacgactg aaggaacacg aagtcaacgg agaaagaagc tggctcaaat
     7321 gatccaggag aatattaaag aagagctagg attgattgct acagtaggtg tcggagataa
     7381 tccccttttg gctaaactag ccttagacaa cgaagcaaag cataatgaag gatttgttgc
     7441 ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat attcctgaaa tgacggactt
     7501 ttggggaatt ggctctcgaa tgaaaaaaag actcaatcag atggggatta tgagcattag
     7561 agatcttgct aactggaatc cttatacgat taagaataga ctaggtgtga ttggcttaca
     7621 gctgtacttc catgctaatg ggatcgatcg aacagatata gcaatcccac cagaaccgac
     7681 aaaggaaaaa tcatatggaa attcacaagt gttaccacga gattatacta gaagaaatga
     7741 aatcgagctg gtggtaaaag aaatggcgga acaagtagcg atacgcatta ggcagcataa
     7801 ctgtaaaaca ggttgtgtcc atttacacat tggcacgtcg atcttagaaa cgagaccagg
     7861 gttttcacac cagatgaaga tccctatcac agataataca aaggaattgc agaattattg
     7921 tcttttcttg tttgataagt attatgaagg acaagaagta aggcatgttg gaataaccta
     7981 tagcaagctt gtgtacaccg attcgcttca gctggattta tttagtgatc cacaaaagca
     8041 aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat atggttttac
     8101 gtcgattgta catgcttctt caatgttaga aggcgctaga agcattacta gaagcacact
     8161 tgttggtggt catgcgggcg gaaatggggg aattaaaaat gattgattac aatgatcgtg
     8221 gacgtttaaa gtgggcaggg ttttatttat ctgatcatac tgaagaaatt gatgccgaca
     8281 atgaacaacg aaccagtcaa aatttagcaa aagaacaaat gacgactgaa gagattgcgg
     8341 aagtattgaa cgctgcagtc ataaaaaata gatcagtatc tattcaaaaa gaagaacgaa
     8401 acgctgaagg atactatcca gaagatatca ttggcaagat cttaggtagt gatgagctgg
     8461 ggatctacgt tggaaaagac aaaattcact acgacgaaat acgtcatatc gaattttacc
     8521 aggataataa atggagtcga ttagaatgaa cattatccag caatatgagc taaagtatat
     8581 aacctttgat cagctatcag aagagatttg gggttatggc caacgtttga taaatgaggt
     8641 tggtgttgag cgcttctctt tctatgtaga agctgcagca ggttatcata attttaggtt
     8701 ttatatcttc ccgttatata tataaggtat tttacggatt ctatagagtt atctctatga
     8761 atcctttttt gtttttacaa ataggagtag atactagaat aatgcgtgtg ttaaactatt
     8821 attgcaaacg caataataaa agaaagtagg cttacgcacc atgtctatca atacatttgt
     8881 ttactcgcac ccgatcaatg tttacattat caagaattta ggaatcactg tagaacaatt
     8941 ttgtgagtta tatgcctacc cacaaggaac ggttgcaagc tggataaccc gacaacgtag
     9001 aataaaatca ttacctgctt cattcgttta tgatttaagc ctggcctcta gtttaaacat
     9061 gtctgatgtt tatgaaaagc tcctgtcttt agaaaaagaa tatgattcct tcaagattaa
     9121 acatgataaa aaaataaaaa aacatatata atatcaatca caattgtata atattacgat
     9181 atcttaatat catgatatcg taatattgtt tttttctaag aaaatgcact ataagaaggg
     9241 aattattagt aacaatatag acagtttagt aatgtttaaa ttattctagt tgctgctcgc
     9301 aaaacatatt tcttatcatt aataaaaaat tttaaagtaa taaaaagtga ttattttaat
     9361 ttcgaataac atatgaataa tctttatatc cgaaccgata tatatcttta aaacaaaata
     9421 atcacacaat attacaatat aatgcatata cgatattgca atatcacaat attatgatat
     9481 aattatatta taatataaga tgaatggggg atattttaat ggcaagaata ttgtcagtag
     9541 ccaatcaaaa aggtggagtt tctaaaacta cgactagcgt taatttagca gcatgtttga
     9601 aatttgttta cggtaaaaat gtacttttag tggacattga tcctcaggga aatgctacag
     9661 ataatgtagg tttcgatata gatggaacaa accaacctac aatttatgaa gtattaaaag
     9721 gggaggctga cattacagat gctattctgg actataaagg aattgatgtt ttgccagccg
     9781 acatagccct ttcttctgca gagagggaat ttactcaagt aggaagtgaa cacaggctta
     9841 aaagagtact tcaaccaatc aaggacaact atgattatat tataattgat tgtccaccaa
     9901 gtttaggaat gcttacagtc aacgcattta ctgtttctga tgaaattatc attccagttg
     9961 aagctgcgta tttctcatta aaaggactaa taaagttaag tgaaactatt gaaacggtaa
    10021 aagaatatac gaacgacaag cttgaagtac gaggagttct atttactaag tataataagc
    10081 gttataacat ttctaaagaa atgacgtctg cagcaaccga aatttctaga gtcattggtg
    10141 caaaaatttt caaaacttat attcgtagga caataaccgt tgacgaagcg caagctgctg
    10201 gatcagatct aatcaatttt aataaaccct caacagctga agatgactat aaagcattta
    10261 cagaagaata tttaaaagaa gttggtgatt ttgatggcag aagataatcg cagtcaagct
    10321 gcagtaagta gattactagg aaataaaaat aaaaagaatt ttaaatcaaa agaagtagaa
    10381 gaaatcgaat taaccaagaa aaatataagt aaagtcataa aagatcaagg aagtagtgta
    10441 agaacagcag atagcattcg tataaatcca attctaatgt cagcactcaa atattggacg
    10501 actattgctg aatcagacaa aagtaaacca gacgttatag aagatgcgct attaaagtcc
    10561 atacctgaag aatacttgat tgaaggatat aaactagcaa agaaacaaaa taaaatttaa
    10621 tttacattca atatcacgat atcataatat cgtgatattt tttttataaa aatcagtagt
    10681 atcaaagctg ataagtcgct tatcttcata ttgtaatatt acgatatcgt gatataatat
    10741 tattgtaata tcgtgatata gttactatga gcttaaacct tgaaatagag cggtttgctt
    10801 taatttattt tttaaggtaa actaaaaaaa gataaaaaaa gactactgct ttaacaaagt
    10861 ttggcgactt atcagcagta gaaaaatata tagtggatag atatatttgt ttaattcatt
    10921 gtaacatagt tttgaacaaa acgctatcct acagaaagag gattggct
//
