LOCUS       NZ_CP066455             6303 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 83 plasmid p83_6.3kb, complete
            sequence.
ACCESSION   NZ_CP066455
VERSION     NZ_CP066455.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038084
            Assembly: GCF_016406425.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 243.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/19/2024 03:45:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 41 of 117
            Pseudo Genes (incomplete)         :: 70 of 117
            Pseudo Genes (internal stop)      :: 30 of 117
            Pseudo Genes (multiple problems)  :: 20 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066455.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 83"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p83_6.3kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(124..294)
                     /locus_tag="JCP60_RS15290"
                     /old_locus_tag="JCP60_15290"
     CDS             complement(124..294)
                     /locus_tag="JCP60_RS15290"
                     /old_locus_tag="JCP60_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(310..843)
                     /locus_tag="JCP60_RS15295"
                     /old_locus_tag="JCP60_15295"
     CDS             complement(310..843)
                     /locus_tag="JCP60_RS15295"
                     /old_locus_tag="JCP60_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_107546896.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(864..1814)
                     /locus_tag="JCP60_RS15300"
                     /old_locus_tag="JCP60_15300"
     CDS             complement(864..1814)
                     /locus_tag="JCP60_RS15300"
                     /old_locus_tag="JCP60_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            2490..2576
                     /locus_tag="JCP60_RS15305"
                     /old_locus_tag="JCP60_15305"
     CDS             2490..2576
                     /locus_tag="JCP60_RS15305"
                     /old_locus_tag="JCP60_15305"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2697..3386)
                     /locus_tag="JCP60_RS15310"
                     /old_locus_tag="JCP60_15310"
     CDS             complement(2697..3386)
                     /locus_tag="JCP60_RS15310"
                     /old_locus_tag="JCP60_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3631..4545)
                     /locus_tag="JCP60_RS15315"
                     /old_locus_tag="JCP60_15315"
     CDS             complement(3631..4545)
                     /locus_tag="JCP60_RS15315"
                     /old_locus_tag="JCP60_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_198879587.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4527..4907)
                     /locus_tag="JCP60_RS15320"
                     /old_locus_tag="JCP60_15320"
     CDS             complement(4527..4907)
                     /locus_tag="JCP60_RS15320"
                     /old_locus_tag="JCP60_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5229..5432)
                     /locus_tag="JCP60_RS15325"
                     /old_locus_tag="JCP60_15325"
     CDS             complement(5229..5432)
                     /locus_tag="JCP60_RS15325"
                     /old_locus_tag="JCP60_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(5444..5977)
                     /locus_tag="JCP60_RS15330"
                     /old_locus_tag="JCP60_15330"
     CDS             complement(5444..5977)
                     /locus_tag="JCP60_RS15330"
                     /old_locus_tag="JCP60_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     Typing          complement(865..1813)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.057"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3635..4545)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.408"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgggttgt tatttttaat aaaatatgat aaatttttgg tagcgcttca taagcgcgtt
       61 gctttttcaa gaataaacac tcattatttt ttaactggct gctcgaaaga gcagtctttt
      121 ttattattta tctgtttctt ttacaaacaa ttcttcgtta ggactgttgt aataatttgg
      181 attcaatttc aattcaccat gtttattccc tttattgatc actgacaata acatcggact
      241 gaatcctaga attcctagta aaatagaacc gaatagaaga ttttttcttg tcatgaatgt
      301 tccctccagt tatttccaaa gttgccacca ttttttttca gtatcaattt gtttgtctat
      361 tgtttctgtt gcttcagaaa gagtcgttga gtcattctct ggagcttctt ctgtataagt
      421 caactgtagt ttttcttgca gtttttcgat ttgtttgttg gcttgtaggg tgagttgttg
      481 ttgctggtcg agtagttttt gtatatgaga attattttgc tctttgaggg caagttgttc
      541 tcgtaaaaat tcattttctt cacgcaactg atctagcaac ttgtttgcaa ccacgttttt
      601 gtttggtata taagcatttt tatcgttgtt ttgttcatgt ttatgcaact tatctagcaa
      661 cctttgattg tctaagttgc gagcaagttg caatactcca gaacgcaata caaccttata
      721 accacgctca tttttagcta tcatggaaga atctaatttt tggatatgtt tcgatattgc
      781 ttgcctactc atcttaaaaa gatctgacaa ttcctttatt gtaaaggttt cttgtggttg
      841 catactggtt gcctcctagg ttgctactgg aataaattat ctaaatcaga taataaactt
      901 tttttctctt ttttcgaaag tgcggactct gtttgttttt caagagcttc tttctctttt
      961 tctagttgtt tgatttgttg gaggagttca agttctcgtt tcttctgttt gattttttcg
     1021 ttaattgttg tagcgggttc ttcgtattct ttttgtttat cagctaaagc caatgcttta
     1081 ttttgttctt ctttggttgg tgcatgttta ttaaaatcat ctgctttttt gatttctggt
     1141 ttaaacgtaa attgatagcc aacgacaggt tttcctcgcc cttttccgta tttctttttg
     1201 attgataatc ctttgaagat tgcggttaat tcctgtcgta taggctttat aacacgatta
     1261 tcaacgtttg cttggttgtt ccagtaactt tttggaatat ctaatagttc atgaaaatcc
     1321 tctttagaaa aataagcaaa acctttagtt cgatattgtt ttagtaatcg aaacatagtc
     1381 tttgcatagc tactttgtaa ttcgttaaat tgttggagtg aatagcgaac ccactcatct
     1441 aaattgttta gtaaaggtaa agctttttcg taaacttgta tgtctacata tggatcttta
     1501 gcagttccac gtattttaaa ctcactaaac ataacgaatc tatctatatc gagcccatta
     1561 gcacttcttt taccaaaacg taaatccatt aatttatcat aagtcatttc taaatcatta
     1621 atgaaacgat tattagcagt tgctttataa ttacttaaat cttttagttg gtcaaaagta
     1681 agacggactg ttttatcgcc tttttcgcgc attctagaca caatagagaa aaatagattc
     1741 atttcaactg gtgtaaattt ccgcaacgga atggtattta attcatgatg gtatttcacg
     1801 atttcatttg ccaaagatat tcctccttac tccttcaatg gatacagacg ttataaacaa
     1861 cgttttgtct gtgataatgc atattataac atacgtgagt gcaaaaaaag agtttaaaag
     1921 cagacaaaat gtagtttaaa agcagacaaa atgtagttta aaagcagaca aaatgtagtt
     1981 taaaagcaga caaaatgtag tttataaaac gctgtatcca ttgaaggagt aaggacacag
     2041 ccgacggtaa agaggtttaa aaaaaggttt aaaaagagat tataaaagag ggtgacaaaa
     2101 tttcccgcct aaaaaccact tcttttttct accaacaggt tcgcgcattt ttcaaaactg
     2161 ccaattcaaa accttgctcc ctacggtcgc taaaaaagtg tgatcgctat gctctcacat
     2221 tgtactgcgc agtcgcctaa aggctccttt gctaaaaaca agtcagtaac ggaggtgggg
     2281 gaagcctctg atagctcgtc taagtcgttt taagacagtc ttgggtaaaa aaccttatct
     2341 tgttcttaaa gcgccttaca ggcggtcagg gcaagcccta acaacccgaa aataagggat
     2401 acttaaaaac accacacaga ttttattttg taaggtataa taacttgtcg cctatccgac
     2461 agtcttgcga ctggaaggag gtgtatctaa tgtttgaacg gttcatttgc ccccttcttg
     2521 tagaagtttg tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc
     2581 aataaaggcg acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc
     2641 gtgtatctaa tgtttgatca tttgcaggtt cagtttaaca tgattgctaa tcaaattcaa
     2701 ttttgttgtt gctgatccaa ccattccttg acactttcta gataatttaa tgccgtttct
     2761 tttttcgttg gattttgtat gtttgttgct tgctgaagca gtgtttcata acgttcgtat
     2821 gtgaccgatt gttgccgttc ttttcgttct tgttcactgg ttgaaaaagg gattgtatcg
     2881 ctaaataagt tacaacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt
     2941 tttacttgct catatttttc ttgtacctct ttttgctgtt tttctggtaa gttttgataa
     3001 aaacgagtga gttttttcat gtttttatct gttaaacgtt tctcttgatc cacaaaaata
     3061 ttgttcatgt cctcataaca agcggtaaat tcctccattt tttgtgctaa tgcttgctct
     3121 tttttctttt tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatgctt
     3181 cttgaaacac gtttattacc ctctaaaacg ccgaataaac cacttaaaac gattgataag
     3241 gcaaatataa ccccgaccaa taaaaatcgc ttaaatcgaa ttttagagcc ttttaatgaa
     3301 tatcttgttt gataagcctt atagctaagc caaataattc ctaaaatact ggcagaaatg
     3361 agtagatagg gtaaatagaa tgccatgtct gtttattctc cccataccaa ccaaaaggct
     3421 aataatgtta gtactaagca acataagggg gcaagaatat ccatcgatcg ttgaattaaa
     3481 gccttttttt gttcttggtt tcgctggtct aatagcgctt ggtggagttt tttgacttgt
     3541 gaatccatcg tagtttgtat cctttcttgg gtataaggcg caagaaaggc acgttgtctc
     3601 tttatttcgt tttcgatgtc ttttaacgtt ttagagcgat aattcttgtt ggtggtctgt
     3661 tcctcgttca acgtgttgtt catggtttct tcgagtcggt ctaatttccg attgttcagg
     3721 cgtaaaagtt cgttgttctc ttcgtacttc ttctgcagtt tcgcgaatgc tagctcgagc
     3781 tcttcgtagc tcgttggttc gttgttgatt gtaggttcgt tctcgtctgt ccagctcatg
     3841 taaaatcgtc tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt
     3901 gtggaccaat tcgtaagtaa aattttttcc gcgattcaag caatgaatgc cgaatgtttt
     3961 aagtgaatca caaaattggg tatacgttcg agtttttttg tttttcattg tttgatcaat
     4021 cgcaaaccga atttcgtctt tccaaaccgg ttcatttttt tcttttaatt taatttcaga
     4081 catggttcgt ttttcggttt tctttcctcg ttccgtgatt gttaagtcat gttcaagaca
     4141 aatctcgtca tttaggtcat aacatttgtt taaaaatgct tgcccatggg cttgaaattt
     4201 tttcccagtt tcaagattaa cggagttaat gacaatgtga ttatgaatat ggtctttgtc
     4261 tgtatgtgtg taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc
     4321 tagttgattg gctttttcag cggtaatttc tcctggtcga aaggactgaa tcaataagtg
     4381 tgcttgtaca cgatcgtctt ttccataaag cgtgcgtatt tgtttcattt cacttttggc
     4441 ataatcaatg tcacagttta ttcctgtttt gacggttgct cgtggttctg cgtaattgat
     4501 tgctcggcta catgaagtgg ttcgacttac tttaactgtt gccatatctt ttgtagctcc
     4561 ttttgcatga tttttaggtt ttcggataaa cgttgagcat catcaggagc aacgtcgtgt
     4621 tggtgtgtat tgcaccactt ggcaatttga ttgatattcc ctccagcttt ggctaaatcg
     4681 cgcgcgattg cctgggcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt
     4741 gccttggctt tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc
     4801 tttgcaaatt cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta
     4861 tattttttag aatgatcgct agccaaattt tgatgttgtt cgctcataag tcccaccctt
     4921 tcagtacgtc ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaatgag
     4981 taaagctcac taaaagggtt tggcaagctc tatatttgct agccaaatac ctccgtattt
     5041 ggtgcaaata accgcttgtc aaaggagcgc aagctccccc ttgaatcccc ctcaaaatcc
     5101 acgctccaca atacaaaaaa aggctagcat ttgctagcca ctttttgtaa agtttcgctt
     5161 atttcaccga aggtgaaatt gttgctttgt aatattcaaa caaccctatt cttctaaaat
     5221 gtatcgattt attttttttt agactttatt atgtctaaga caactaagat taacactaaa
     5281 aataaaccag atttgtataa ttgccctccg cttagcatat taacaattat agcaacagct
     5341 atagcaaata taaaaatacg aatagtagct atttgcggat tctttttagt aaatgttttt
     5401 aatgatttta atgatttaga tatcaaaaac atcactcctt aacttataac gttgaatatc
     5461 tagtagctac aactcttcca gctgcatcat atcccctttg gtaataaaat ccaatccagc
     5521 cattaccatt tttgtcccat tcttttgcaa ctgcattccc tggagaagca ccttttttag
     5581 tccaacttgc acctgttaac aaagcaccaa ttgcccaacc tgggatacca aacatagaag
     5641 tgctagcttt ccctcctgcc caaaaggcag cattaatagc attagcagct actccagtgg
     5701 ttacagcaat atttgtatat tcctttttgc cccatcttgc aactgcagct cttgtcatga
     5761 tatctgaaga atttgcaaaa tcttccgcac tataaccatc ctcatattta gttacgacca
     5821 taccatcttc agtgtactca ataactacat tttcagtagc attgttttcg tttaatgata
     5881 ccacttggga attttctgaa gcataaacta tagtagttga cggtaaaaca actgccccaa
     5941 tagttaacaa agctacccct aaagtaaatt ttttcataga atttcctcct gatacagaaa
     6001 aatatataga aaacgttttc tttgtgaaaa tcataaaaca aaaggtatag gcttgtcaat
     6061 attttattta gtaaagataa tttatatttt tgataatgtg gtatagttag aatgcattag
     6121 gaaaatatgt tatattaaat tcttgttgac ataatgtaca ctatgccaag tagcccttgt
     6181 atcaaccgat acagaggctc actttgtgat tttcgattgg tctaggtcaa aagctctttt
     6241 taagcttgat tttctgcggt tgcttctttc cgtttctttg tgaattttta ccagtcattt
     6301 ttt
//
