LOCUS       NZ_CP066447             6312 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 71_2 plasmid p71_2_6.3kb,
            complete sequence.
ACCESSION   NZ_CP066447
VERSION     NZ_CP066447.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038083
            Assembly: GCF_016406525.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:51:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,192
            CDSs (total)                      :: 3,100
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 45 of 129
            Pseudo Genes (incomplete)         :: 70 of 129
            Pseudo Genes (internal stop)      :: 37 of 129
            Pseudo Genes (multiple problems)  :: 20 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066447.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 71_2"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p71_2_6.3kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(join(5594..6312,1..232))
                     /locus_tag="JCP66_RS15735"
                     /old_locus_tag="JCP66_15735"
     CDS             complement(join(5594..6312,1..232))
                     /locus_tag="JCP66_RS15735"
                     /old_locus_tag="JCP66_15735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            908..994
                     /locus_tag="JCP66_RS15740"
                     /old_locus_tag="JCP66_15740"
     CDS             908..994
                     /locus_tag="JCP66_RS15740"
                     /old_locus_tag="JCP66_15740"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1115..1804)
                     /locus_tag="JCP66_RS15745"
                     /old_locus_tag="JCP66_15745"
     CDS             complement(1115..1804)
                     /locus_tag="JCP66_RS15745"
                     /old_locus_tag="JCP66_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1811..2089)
                     /locus_tag="JCP66_RS16020"
     CDS             complement(1811..2089)
                     /locus_tag="JCP66_RS16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(2049..2963)
                     /locus_tag="JCP66_RS15750"
                     /old_locus_tag="JCP66_15750"
     CDS             complement(2049..2963)
                     /locus_tag="JCP66_RS15750"
                     /old_locus_tag="JCP66_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2945..3325)
                     /locus_tag="JCP66_RS15755"
                     /old_locus_tag="JCP66_15755"
     CDS             complement(2945..3325)
                     /locus_tag="JCP66_RS15755"
                     /old_locus_tag="JCP66_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_198849540.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3656..3859)
                     /locus_tag="JCP66_RS15760"
                     /old_locus_tag="JCP66_15760"
     CDS             complement(3656..3859)
                     /locus_tag="JCP66_RS15760"
                     /old_locus_tag="JCP66_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(3871..4404)
                     /locus_tag="JCP66_RS15765"
                     /old_locus_tag="JCP66_15765"
     CDS             complement(3871..4404)
                     /locus_tag="JCP66_RS15765"
                     /old_locus_tag="JCP66_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(4854..5024)
                     /locus_tag="JCP66_RS15770"
                     /old_locus_tag="JCP66_15770"
     CDS             complement(4854..5024)
                     /locus_tag="JCP66_RS15770"
                     /old_locus_tag="JCP66_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5040..5573)
                     /locus_tag="JCP66_RS15775"
                     /old_locus_tag="JCP66_15775"
     CDS             complement(5040..5573)
                     /locus_tag="JCP66_RS15775"
                     /old_locus_tag="JCP66_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     Typing          complement(2053..2963)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatccattaa tttatcataa gtcatttcta aatcattaat gaaacgatta ttagcagttg
       61 ctttataatt acttaaatct tttagttggt caaaagtaag acggactgtt ttatcgcctt
      121 tttcgcgcat tctagacaca atagagaaaa atagattcat ttcaactggt gtaaatttcc
      181 gcaacggaat ggtatttaat tcatgatggt atttcacgat ttcatttgcc aaagatattc
      241 ctccttactc cttcaatgga tacagacgtt ataaacaacg ttttgtctgt gataatgcat
      301 attataacat acgtgagtgc aaaaaaagag tttaaaagca gacaaaatgt agtttaaaag
      361 cagacaaaat gtagtttaaa agcagacaaa atgtagttta aaagcagaca aaatgtagtt
      421 tataaaacgc tgtatccatt gaaggagtaa ggacacagcc gacggtaaag aggtttaaaa
      481 aaaggtttaa aaagagatta taaaagaggg tgacaaaatt tcccgcctaa aaaccacttc
      541 ttttttctac caacaggttc gcgcattttt caaaactgcc aattcaaaac cttgctccct
      601 acggtcgcta aaaaagtgtg atcgctatgc tctcacattg tactgcgcag tcgcctaaag
      661 gctcctttgc taaaaacaag tcagtaacgg aggtggggga agcctctgat agctcgtcta
      721 agtcgtttta agacagtctt gggtaaaaaa ccttatcttg ttcttaaagc gccttacagg
      781 cggtcagggc aagccctaac aacccgaaaa taagggatac ttaaaaacac cacacagatt
      841 ttattttgta aggtataata acttgtcgcc tatccgacag tcttgcgact ggaaggaggt
      901 gtatctaatg tttgaacggt tcatttgccc ccttcttgta gaagtttgtg tctctttatt
      961 cagttattgg ttgaataacc gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga
     1021 aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgatcatt
     1081 tgcaggttca gtttaacatg attgctaatc aaattcaatt ttgttgttgc tgatccaacc
     1141 attccttgac actttctaga taatttaatg ccgtttcttt tttcgttgga ttttgtatgt
     1201 ttgttgcttg ctgaagcagt gtttcataac gttcgtatgt gaccgattgt tgccgttctt
     1261 ttcgttcttg ttcactggtt gaaaaaggga ttgtatcgct aaataagtta caacaagctt
     1321 cttctgtctg cgtatcttta aaatattgca cgtctttttt tacttgctca tatttttctt
     1381 gtacctcttt ttgctgtttt tctggtaagt tttgataaaa acgagtgagt tttttcatgt
     1441 ttttatctgt taaacgtttc tcttgatcca caaaaatatt gttcatgtcc tcataacaag
     1501 cggtaaattc ctccattttt tgtgctaatg cttgctcttt tttcttttta tcacatgctg
     1561 cttcatactt ttgagtgata tcccctaata aaatgcttct tgaaacacgt ttattaccct
     1621 ctaaaacgcc gaataaacca cttaaaacga ttgataaggc aaatataacc ccgaccaata
     1681 aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata tcttgtttga taagccttat
     1741 agctaagcca aataattcct aaaatactgg cagaaatgag tagatagggt aaatagaatg
     1801 ccatgtctgt ttattctccc cataccaacc aaaaggctaa taatgttagt actaagcaac
     1861 ataagggggc aagaatatcc atcgatcgtt gaattaaagc ctttttttgt tcttggtttc
     1921 gctggtctaa tagcgcttgg tggagttttt tgacttgtga atccatcgta gtttgtatcc
     1981 tttcttgggt ataaggcgca agaaaggcac gttgtctctt tatttcgttt tcgatgtctt
     2041 ttaacgtttt agagcgataa ttcttgttgg tggtctgttc ctcgttcaac gtgttgttca
     2101 tggtttcttc gagtcggtct aatttccgat tgttcaggcg taaaagttcg ttgttctctt
     2161 cgtacttctt ctgcagtttc gcgaatgcta gctcgagctc ttcgtagctc gttggttcgt
     2221 tgttgattgt aggttcgttc tcgtctgtcc agctcatgta aaatcgtctc cttttcgtaa
     2281 tcctttccta gtttgttgga tcgtactttt ttctttttgt ggaccaattc gtaagtaaaa
     2341 ttttttccgc gattcaagca atgaatgccg aatgttttaa gtgaatcaca aaattgggta
     2401 tacgttcgag tttttttgtt tttcattgtt tgatcaatcg caaaccgaat ttcgtctttc
     2461 caaaccggtt catttttttc ttttaattta atttcagaca tggttcgttt ttcggttttc
     2521 tttcctcgtt ccgtgattgt taagtcatgt tcaagacaaa tctcgtcatt taggtcataa
     2581 catttgttta aaaatgcttg cccatgggct tgaaattttt tcccagtttc aagattaacg
     2641 gagttaatga caatgtgatt atgaatatgg tctttgtctg tatgtgtgta aatcgcgatt
     2701 tggtggttag gggctatttt tttcgcatac tctgccccta gttgattggc tttttcagcg
     2761 gtaatttctc ctggtcgaaa ggactgaatc aataagtgtg cttgtacacg atcgtctttt
     2821 ccataaagcg tgcgtatttg tttcatttca cttttggcat aatcaatgtc acagtttatt
     2881 cctgttttga cggttgctcg tggttctgcg taattgattg ctcggctaca tgaagtggtt
     2941 cgacttactt taactgttgc catatctttt gtagctcctt ttgcatgatt tttaggtttt
     3001 cggataaacg ttgagcatca tcaggagcaa cgtcgtgttg gtgtgtattg caccacttgg
     3061 caatttgatt gatattccct ccagctttgg ctaaatcgcg cgcgattgcc tgggcaatgt
     3121 ctggcgccac tttcggtgtc accagacgcg cgttttgtgc cttggctttc acgaaagccg
     3181 gcacactcat ttgcaaagct tcagctgact gcttcagctt tgcaaattcg gattcgctca
     3241 ctcgaaaact gatttgtttt ggctcggatc gataggtata ttttttagaa tgattgctag
     3301 ccaaatattg attttgttcg ctcataagtc ccaccctttc agtacgtcct acggacgtat
     3361 cgttcgcaat accattgtac ctaaaaaggt acaataagta aagctcacta aagctcacta
     3421 aagggcttgg caagctctat atttgctagc caaatacctc cgtatttggt gcaaataacc
     3481 gcttgtcaaa ggagcgcaag ctcccccttg aatccccctc aaaatccacg ctccacaata
     3541 caaaaaaagg ctagcatttg ctagccactt tttgtaaagt ttcgcttatt tcaccgaagg
     3601 tgaaattgtt gctttgtaat attcaaacaa ccctattctt ctaaaatgta tcgatttatt
     3661 tttttttaga ctttattatg tctaagacaa ctaagattaa cactaaaaat aaaccagatt
     3721 tgtataattg ccctccgctt agcatattaa caattatagc aacagctata gcaaatataa
     3781 aaatacgaat agtagctatt tgcggattct ttttagtaaa tgtttttaat gattttaatg
     3841 atttagatat caaaaacatc actccttaac ttataacgtt gaatatctag tagctacaac
     3901 tcttccagct gcatcatatc ccctttggta ataaaatcca atccagccat taccattttt
     3961 gtcccattct tttgcaactg cattccctgg agaagcacct tttttagtcc aacttgcacc
     4021 tgttaacaaa gcaccaattg cccaacctgg gataccaaac atagaagtgc tagctttccc
     4081 tcctgcccaa aaggcagcat taatagcatt agcagctact ccagtggtta cagcaatatt
     4141 tgtatattcc tttttgcccc atcttgcaac tgcagctctt gtcatgatat ctgaagaatt
     4201 tgcaaaatct tccgcactat aaccatcctc atatttagtt acgaccatac catcttcagt
     4261 gtactcaata actacatttt cagtagcatt gttttcgttt aatgatacca cttgggaatt
     4321 ttctgaagca taaactatag tagttgacgg taaaacaact gccccaatag ttaacaaagc
     4381 tacccctaaa gtaaattttt tcatagaatt tcctcctgat acagaaaaat atatagaaaa
     4441 cgttttcttt gtgaaaatca taaaacaaaa ggtataggct tgtcaatatt ttatttagta
     4501 aagataattt atatttttga taatgtggta tagttagaat gcattaggaa aatatgttat
     4561 attaaattct tgttgacata atgtacacta tgccaagtag cccttgtatc aaccgataca
     4621 gaggctcact ttgtgatttt cgattggtct aggtcaaaag ctctttttaa gcttgatttt
     4681 ctgcggttgc ttctttccgt ttctttgtga atttttacca gtcatttttt actgggttgt
     4741 tatttttaat aaaatatgat aaatttttgg tagcgcttca taagcgcgtt gctttttcaa
     4801 gaataaacac tcattatttt ttaactggct gctcgaaaga gcagtctttt ttattattta
     4861 tctgtttctt ttacaaacaa ttcttcgtta ggactgttgt aataatttgg attcaatttc
     4921 aattcaccat gtttattccc tttattgatc actgacaata acatcggact gaatcctaga
     4981 attcctagta aaatagaacc gaatagaaga ttttttcttg tcatgaatgt tccctccagt
     5041 tatttccaaa gttgccacca ttttttttca gtatcaattt gtttgtctat tgtttctgtt
     5101 gcttcagaaa gagtcgttga gtcattctct ggagcttctt ctgtataagt caactgtagt
     5161 ttttcttgca gtttttcgat ttgtttgttg gcttgtaggg tgagttgttg ttgctggtcg
     5221 agtagttttt gtatatgaga attattttgc tctctgaggg caagttgttc tcgtaaaaat
     5281 tcattttctt cacgcaactg atctagcaac ttgtttgtaa ccacgttttt gcttggtata
     5341 ttaacatttt tatcgttgtc ttgttcgtac tcgtgcaacc tatctagcaa catttggttg
     5401 cctaagttgc gagcaagttg caatactcca gaacgcaata caaccttata accacgctca
     5461 tttttagcta tcatggaaga atctaatttt tggatatgtt tcgatattgc ttgcctactc
     5521 atcttaaaaa gttctgacaa ttcctttatt gtaaaggttt cttgtggttg catactggtt
     5581 gcctcctagg ttgctactgg aataaattat ctaaatcaga taataaactt tttttctctt
     5641 ttttcgaaag tgcggactct gtttgttttt caagagcttc tttctctttt tctagttgtt
     5701 tgatttgttg gaggagttca agttctcgtt tcttctgttt gattttttcg ttaattgttg
     5761 tagcaggttc ttcgtattct ttttgtttat cagctaaagc caatgcttta ttttgttctt
     5821 ctttggttgg tgcatgttta ttaaaatcat ctgctttttt gatttctggt ttaaacgtaa
     5881 attgatagcc aacgacaggt tttcctcgcc cttttccgta tttctttttg attgataatc
     5941 ctttgaagat tgcggttaat tcctgtcgta taggctttat aacacgatta tcaacgtttg
     6001 cttggttgtt ccagtaactt tttggaatat ctaatagttc atgaaaatcc tctttagaaa
     6061 aataagcaaa acctttagtt cgatattgtt ttagtaatcg aaacatagtc tttgcatagc
     6121 tactttgtaa ttcgttaaat tgttggagtg aatagcgaac ccactcatct aaattgttta
     6181 gtaaaggtaa agctttttcg taaacttgta tgtctacata tggatcttta gcagttccac
     6241 gtattttaaa ctcactaaac ataacgaatc tatctatatc gagcccatta gcacttcttt
     6301 taccaaaacg ta
//
