LOCUS       NZ_CP066440             7012 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 66 plasmid p66_7kb, complete
            sequence.
ACCESSION   NZ_CP066440
VERSION     NZ_CP066440.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038081
            Assembly: GCF_016406405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 131.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/19/2024 03:34:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,204
            CDSs (total)                      :: 3,112
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 43 of 119
            Pseudo Genes (incomplete)         :: 67 of 119
            Pseudo Genes (internal stop)      :: 32 of 119
            Pseudo Genes (multiple problems)  :: 19 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066440.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 66"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p66_7kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            1..945
                     /locus_tag="JCP89_RS15825"
                     /old_locus_tag="JCP89_15815"
     CDS             1..945
                     /locus_tag="JCP89_RS15825"
                     /old_locus_tag="JCP89_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..1502
                     /locus_tag="JCP89_RS15830"
                     /old_locus_tag="JCP89_15820"
     CDS             966..1502
                     /locus_tag="JCP89_RS15830"
                     /old_locus_tag="JCP89_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_198879584.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1518..1688
                     /locus_tag="JCP89_RS15835"
                     /old_locus_tag="JCP89_15825"
     CDS             1518..1688
                     /locus_tag="JCP89_RS15835"
                     /old_locus_tag="JCP89_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            2092..3246
                     /locus_tag="JCP89_RS15840"
                     /old_locus_tag="JCP89_15830"
     CDS             2092..3246
                     /locus_tag="JCP89_RS15840"
                     /old_locus_tag="JCP89_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_002348708.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            3219..3662
                     /locus_tag="JCP89_RS15845"
                     /old_locus_tag="JCP89_15835"
     CDS             3219..3662
                     /locus_tag="JCP89_RS15845"
                     /old_locus_tag="JCP89_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            3964..4344
                     /locus_tag="JCP89_RS15850"
                     /old_locus_tag="JCP89_15840"
     CDS             3964..4344
                     /locus_tag="JCP89_RS15850"
                     /old_locus_tag="JCP89_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002348702.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4326..5240
                     /locus_tag="JCP89_RS15855"
                     /old_locus_tag="JCP89_15845"
     CDS             4326..5240
                     /locus_tag="JCP89_RS15855"
                     /old_locus_tag="JCP89_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002348701.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5200..5478
                     /locus_tag="JCP89_RS16245"
     CDS             5200..5478
                     /locus_tag="JCP89_RS16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            5480..6169
                     /locus_tag="JCP89_RS15860"
                     /old_locus_tag="JCP89_15850"
     CDS             5480..6169
                     /locus_tag="JCP89_RS15860"
                     /old_locus_tag="JCP89_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4327..5237
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagaactt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctagc aacttaggta
     1141 accaaaggtt actagatagg ttgcatgagt atgaaaaaga caacgataaa aacatttata
     1201 tatcaagcaa aaacgtggtt gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaactttcc cttaaagagc aaaataattc taatatgcaa aaattactcg
     1321 accagcaaca acagctcacc ctacaggcaa atcaacagat cgaaaaactg caagaacagt
     1381 tacaattagt ttatacggaa gaaactccag aagatgaatc aactactatt ctttctgaag
     1441 aaacagaatc gatagaaaaa cacacaaaga agaagcaaaa atggtggcaa ctttggaaat
     1501 aactggaggg aatattcatg acaagaaaaa atcttctatt cggttctatt ttactaggaa
     1561 ttctaggctt cagtccgatg ttattgtcag tgatcaataa agggaataaa catggtgaat
     1621 tgaaattgaa tccaaattat tacaacagtc ctaacgaaga attgtttgta aaagaaacgg
     1681 ataaataata aaaaagactg ctctttcgag cagccagtta aaaaataatg agtgtttatt
     1741 cttgaaaaag caacgcgctt atgaagcgct accaaaaatt tatcatattt tatgaaaaat
     1801 aacaccccag taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaaccgca
     1861 gaaaatcaag cttaaaaaga gcttttgacc tagaccaatc gaaaatcaca aagtgagcct
     1921 ctgtatcggt tgatacaagg gctacttggc atagtgtaca ttatgttaac taacgctata
     1981 aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt tgttatcgca
     2041 tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg cgtgattatt
     2101 ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat tggaattttt
     2161 agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat caactcccaa
     2221 ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc cataacttca
     2281 attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg ttcgccattt
     2341 aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt attttcagac
     2401 gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg agaatattca
     2461 ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag taatataaca
     2521 gatacaacct tattttttca agataatagc atttattatt ccgataatgg acaaattaaa
     2581 ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt tacagaaaaa
     2641 aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa aaaacaaaat
     2701 tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat tatgattaat
     2761 tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt agaagctgct
     2821 atcttatgta acaaaacaga tgtacatgct acttcagcta gagccattat gcattatacc
     2881 tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga tcaaataata
     2941 aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact aaacctttca
     3001 gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc tggttatgga
     3061 aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca aggtggttat
     3121 caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag tgcaaatgca
     3181 ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac cattacaaat
     3241 tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc ttaactagtt
     3301 gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga acttggcaaa
     3361 ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa aatatagaac
     3421 aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat aaaaatttaa
     3481 atataaatga acaagttttc atttacaaag caacaaataa tttactaaca ctgcgctcta
     3541 ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc gtatttattt
     3601 tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt aaagctgatt
     3661 gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa caagcgaaac
     3721 tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt gggttttgag
     3781 ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc aaatacgtag
     3841 gtatttggct agcaaatata gagcttgcca agcccttttg gtgagcttta ctcattgtgc
     3901 ctttttaggt acaatggtat tgcgaacgat acgtccgtag gacgtactga aagggtggga
     3961 cttatgagcg aacaaaatca aaatttggct agcgatcatt ctaaaaaata tacctatcga
     4021 tccaagccaa aacaaatcag ttttcgagtg agcgaatctg aatttgcaaa gctgaagcag
     4081 tcagctgaag ctttgcaaat gagtgtgccg gctttcgtga aagccaaggc acaaaacgcg
     4141 cgtctggtga caccgaaagt ggcgccagac attgcccagg caatcgcgcg cgatttagcc
     4201 aaagctggag ggaatatcaa tcaaattgcc aagtggtgca atacacacca acatgacgtt
     4261 gctcctggag acgcccaacg tttatccgaa aacctaaaaa tcatgcaaaa ggagctacaa
     4321 aagatatggc aacagttaaa gtaagtcgaa ccacttcctg tagccgagca atcaattacg
     4381 cagaaccacg agcaaccatt aaaacaggaa taaactgtga cgtcgattat gccaaaagtg
     4441 aaatgaaaca aattcgcatg ctgtatggaa aagacgaccg tgtacaggca cacttattga
     4501 ttcaatcttt tcgaccagga gaaattaccg ctgaaaaagc taatcaacta ggggcagagt
     4561 atgcaaaaaa aatagccccc gatcaccaaa cagctattta cacacataca gacaaagacc
     4621 atattcataa tcacattgtc attaactccg ttaatctgga gactggaaaa aaatttcaag
     4681 ctcatggtca agcatttcta aacaaatgtt atgacctaaa tgacgagatt tgtcttgcac
     4741 ataacttacc aatcactgag cgaggaaaga aaccagaaaa acgaaccatg tctgaaataa
     4801 aattaaaaga aaaaaatgaa cctgtttgga aagacgaaat tcggattgcg atcgatcaaa
     4861 caatgaaaaa caaaaaaacc agaacctatg accaattttg taattccctc aaaacattgg
     4921 ggattcattg cttcaatcgc gggaaaaatt tcacttacga attgatccac aaaaagaaaa
     4981 aagtacgatc caacagacta ggaaaggact acgaaaagga gacgatttta catgagctgg
     5041 acagacgaga acgaacctat aaccaacagc gaaccaacga gctacgaaga gctcgagcta
     5101 gcattcgcga aactgcagaa gaagtacgaa gagaacaacg aactcttacg cctgaacaac
     5161 cggaaattag accggctcga agaaaccatg aacaacacgt tgaaagagga acagaccacc
     5221 aacaagaatt atcgctctaa aacgttaaaa gacatagaaa acgaaataaa gaaacaacgt
     5281 gcttttctcg ttccttatac gcaagaaagg atacaaacta cgattgattt acaagttaaa
     5341 aaactccatc aagagctatt agaccagcga aataaggaac agaaaaaggt gcttattcaa
     5401 cgatcattgg attttcttgc ccccttatgt tgcttagtcc tgacactgtt cgcattttgg
     5461 ttagtatggg gaggctaaaa tggcatttta tttaccctat ctactcattt ttgttagtat
     5521 ttcaggaagt atttggctca tctataaaat ttttcaaaca agatattcat taaaaggctc
     5581 taaaattcga tttaagcgat ttttctttct tggctgtata ttttccttga taatcgtttc
     5641 aagcggctta ttcggcgttt tagagggaaa taagcgcgtt tctagaagta ttttattagg
     5701 aaatgtcact caaaagtatg aatcagcgcg tgataaaaag aaaaaagagc aagcattagc
     5761 acaaaaaatg gaagagttta ccacttgtta tgaggaaatg aacgatattt ttgtgaatca
     5821 agaaaaacgt ttgacggata aaaacatgga aaaactcact cgtctttatc aaaacttgcc
     5881 agaaaaacag caaaaagagg ttcaagacaa ctatgagcga accaaaaaag acgtgcaata
     5941 tgtgaaagat acgaaagtgg aagaagcttg tagtgattta tttggagata caattccttt
     6001 ttcaactagc gaacaagaac gaaaagaacg acaacaatca gtcacatatg aacgctatga
     6061 aatcttgctt caacaagcaa ttaacataca aaatctgact aaaaaagaaa cagctttaaa
     6121 ttatttaaaa agcgtcaagg aatggttaga tcagcaacag caaaattgaa tttgatcggc
     6181 aatcatgtta aactaaacat gcaaatgatc aaacattaga tacacgaaag ccccagagag
     6241 gtcggattct ctggggcttt ttttcaggtt gatgtcgcct ttattgattt tacttcttgt
     6301 ttcggttatt caaccaataa ctgaataaag agacacaaac ttctataaga agggggcaaa
     6361 tgaacaattc aaacattaga tacacctcct tccagtcgca agactgtcgg ataggcgaca
     6421 agttattata tcttaaaaaa ttaaacttgt gtggtgtttt taagtatcgc ttattttagg
     6481 gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa agctagataa ggatttttat
     6541 ccaagactgt cttaaaacgg cttagataag cattcacaac cgctcaaagg cctctttacc
     6601 aacatgtttt tagcaaagga gcctttaggc gactgcgcag tacaatgtga gagcgtagcg
     6661 atcacacctt tttggcgacc gtaggaagca aggttttgga ttggcagttt tgaaaaatgc
     6721 gcgaacctgt ttaaagaaaa aagaagtggt ttttaggcgg gaaaattagc ccccctcttt
     6781 ttatatctct ttttaaacct ctttttaaac ctctttaccg taagctgtgt ccttactcta
     6841 ccaatggata cagaatgtta tgaaggacat tttgtctgtt tatgaaggac attttgtctg
     6901 tttatgaagg acattttgtc tgtttatgaa ggacattttg tctgtttgaa cgtcaaatat
     6961 gaatgtcttg acatttaaaa aatgtcattc ataattggat tgaggtgatt at
//
