LOCUS       NZ_CP066439             8194 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 66 plasmid p66_8kb, complete
            sequence.
ACCESSION   NZ_CP066439
VERSION     NZ_CP066439.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038081
            Assembly: GCF_016406405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8194)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 131.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/19/2024 03:34:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,204
            CDSs (total)                      :: 3,112
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 43 of 119
            Pseudo Genes (incomplete)         :: 67 of 119
            Pseudo Genes (internal stop)      :: 32 of 119
            Pseudo Genes (multiple problems)  :: 19 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066439.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 66"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p66_8kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            47..580
                     /locus_tag="JCP89_RS15770"
                     /old_locus_tag="JCP89_15760"
     CDS             47..580
                     /locus_tag="JCP89_RS15770"
                     /old_locus_tag="JCP89_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            592..795
                     /locus_tag="JCP89_RS15775"
                     /old_locus_tag="JCP89_15765"
     CDS             592..795
                     /locus_tag="JCP89_RS15775"
                     /old_locus_tag="JCP89_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            1117..1497
                     /locus_tag="JCP89_RS15780"
                     /old_locus_tag="JCP89_15770"
     CDS             1117..1497
                     /locus_tag="JCP89_RS15780"
                     /old_locus_tag="JCP89_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1479..2393
                     /locus_tag="JCP89_RS15785"
                     /old_locus_tag="JCP89_15775"
     CDS             1479..2393
                     /locus_tag="JCP89_RS15785"
                     /old_locus_tag="JCP89_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2638..3327
                     /locus_tag="JCP89_RS15790"
                     /old_locus_tag="JCP89_15780"
     CDS             2638..3327
                     /locus_tag="JCP89_RS15790"
                     /old_locus_tag="JCP89_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            4210..5160
                     /locus_tag="JCP89_RS15800"
                     /old_locus_tag="JCP89_15790"
     CDS             4210..5160
                     /locus_tag="JCP89_RS15800"
                     /old_locus_tag="JCP89_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            5181..5714
                     /locus_tag="JCP89_RS15805"
                     /old_locus_tag="JCP89_15795"
     CDS             5181..5714
                     /locus_tag="JCP89_RS15805"
                     /old_locus_tag="JCP89_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            5730..5900
                     /locus_tag="JCP89_RS15810"
                     /old_locus_tag="JCP89_15800"
     CDS             5730..5900
                     /locus_tag="JCP89_RS15810"
                     /old_locus_tag="JCP89_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(6165..7313)
                     /gene="tnpB"
                     /locus_tag="JCP89_RS15815"
                     /old_locus_tag="JCP89_15805"
     CDS             complement(6165..7313)
                     /gene="tnpB"
                     /locus_tag="JCP89_RS15815"
                     /old_locus_tag="JCP89_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(7330..7734)
                     /gene="tnpA"
                     /locus_tag="JCP89_RS15820"
                     /old_locus_tag="JCP89_15810"
     CDS             complement(7330..7734)
                     /gene="tnpA"
                     /locus_tag="JCP89_RS15820"
                     /old_locus_tag="JCP89_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     Typing          4212..5160
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1480..2390
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
       61 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
      121 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
      181 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
      241 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
      301 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
      361 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
      421 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
      481 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
      541 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
      601 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
      661 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
      721 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
      781 tctaaaaaaa aataaatcga tacattttag aagaataggg ttgtttgaat attacaaagc
      841 aacaatttca ccttcggtga aataagcgaa actttacaaa aagtggctag caaatgctag
      901 cctttttttg tattgtggag cgtggatttt gagggggatt caagggggag cttgcgctcc
      961 tttgacaagc ggttatttgc accaaatacg gaggtatttg gctagcaaat atagagcttg
     1021 ccaaaccctt ttagtgagct ttactcattg taccttttta ggtacaatgg tattgcgaac
     1081 gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaca tcaaaatttg
     1141 gctagcgatc attctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga
     1201 gtgagcgaat ccgaatttgc aaagctgaag cagtcagctg aagctttgca aatgagtgtg
     1261 ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca
     1321 gacattgccc aggcaatcgc gcgcgattta gccaaagctg gagggaatat caatcaaatt
     1381 gccaagtggt gcaatacaca ccaacacgac gttgctcctg atgatgctca acgtttatcc
     1441 gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacagtt aaagtaagtc
     1501 gaaccacttc atgtagccga gcaatcaatt acgcagaacc acgagcaacc gtcaaaacag
     1561 gaataaactg tgacattgat tatgccaaaa gtgaaatgaa acaaatacgc acgctttatg
     1621 gaaaagacga tcgtgtacaa gcacacttat tgattcagtc ctttcgacca ggagaaatta
     1681 ccgctgaaaa agccaatcaa ctaggggcag agtatgcgaa aaaaatagcc cctaaccacc
     1741 aaatcgcgat ttacacacat acagacaaag accatattca taatcacatt gtcattaact
     1801 ccgttaatct tgaaactggg aaaaaatttc aagcccatgg gcaagcattt ttaaacaaat
     1861 gttatgacct aaatgacgag atttgtcttg aacatgactt aacaatcacg gaacgaggaa
     1921 agaaaaccga aaaacgaacc atgtctgaaa ttaaattaaa agaaaaaaat gaaccggttt
     1981 ggaaagacga aattcggttt gcgattgatc aaacaatgaa aaacaaaaaa actcgaacgt
     2041 atacccaatt ttgtgattca cttaaaacat tcggcattca ttgcttgaat cgcggaaaaa
     2101 attttactta cgaattggtc cacaaaaaga aaaaagtacg atccaacaaa ctaggaaagg
     2161 attacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgaacc tacaatcaac
     2221 aacgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca gaagaagtac
     2281 gaagagaaca acgaactttt acgcctgaac aatcggaaat tagaccgact cgaagaaacc
     2341 atgaacaaca cgttgaacga ggaacagacc accaacaaga attatcgctc taaaacgtta
     2401 aaagacatcg aaaacgaaat aaagagacaa cgtgcctttc ttgcgcctta tacccaagaa
     2461 aggatacaaa ctacgatgga ttcacaagtc aaaaaactcc accaagcgct attagaccag
     2521 cgaaaccaag aacaaaaaaa ggctttaatt caacgatcga tggatattct tgccccctta
     2581 tgttgcttag tactaacatt attagccttt tggttggtat ggggagaata aacagacatg
     2641 gcattctatt taccctatct actcatttct gccagtattt taggaattat ttggcttagc
     2701 tataaggctt atcaaacaag atattcatta aaaggctcta aaattcgatt taagcgattt
     2761 ttattggtcg gggttatatt tgccttatca atcgttttaa gtggtttatt cggcgtttta
     2821 gagggtaata aacgtgtttc aagaagcatt ttattagggg atatcactca aaagtatgaa
     2881 gcagcatgtg ataaaaagaa aaaagagcaa gcattagcac aaaaaatgga ggaatttacc
     2941 gcttgttatg aggacatgaa caatattttt gtggatcaag agaaacgttt aacagataaa
     3001 aacatgaaaa aactcactcg tttttatcaa aacttaccag aaaaacagca aaaagaggta
     3061 caagaaaaat atgagcaagt aaaaaaagac gtgcaatatt ttaaagatac gcagacagaa
     3121 gaagcttgtt gtaacttatt tagcgataca atcccttttt caaccagtga acaagaacga
     3181 aaagaacggc aacaatcggt cacatacgaa cgttatgaaa cactgcttca gcaagcaaca
     3241 aacatacaaa atccaacgaa aaaagaaacg gcattaaatt atctagaaag tgtcaaggaa
     3301 tggttggatc agcaacaaca aaattgaatt tgattagcaa tcatgttaaa ctgaacctgc
     3361 aaatgatcaa acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt
     3421 ttcaggttga tgtcgccttt attgatttta cttcttgttt cggttattca accaataact
     3481 gaataaagag acacaaactt ctacaagaag ggggcaaatg aaccgttcaa acattagata
     3541 cacctccttc cagtcgcaag actgtcggat aggcgacaag ttattatacc ttacaaaata
     3601 aaatctgtgt ggtgttttta agtatccctt attttcgggt tgttagggct tgccctgacc
     3661 gcctgtaagg cgctttaaga acaagataag gttttttacc caagactgtc ttaaaacgac
     3721 ttagacgagc tatcagaggc ttcccccacc tccgttactg acttgttttt agcaaaggag
     3781 cctttaggcg actgcgcagt acaatgtgag agcatagcga tcacactttt ttagcgaccg
     3841 tagggagcaa ggttttgaat tggcagtttt gaaaaatgcg cgaacctgtt ggtagaaaaa
     3901 agaagtggtt tttaggcggg aaattttgtc accctctttt ataatctctt tttaaacctt
     3961 tttttaaacc tctttaccgt cggctgtgtc cttactcctt caatggatac agcgttttat
     4021 aaactacatt ttgtctgctt ttaaactaca ttttgtctgc ttttaaacta cattttgtct
     4081 gcttttaaac tacattttgt ctgcttttaa actctttttt tgcactcacg tatgttataa
     4141 tatgcattat cacagacaaa acgttgttta taacgtctgt atccattgaa ggagtaagga
     4201 ggaatatctt tggcaaatga aatcgtgaaa taccatcatg aattaaatac cattccgttg
     4261 cggaaattta caccagttga aatgaatcta tttttctcta ttgtgtctag aatgcgcgaa
     4321 aaaggcgata aaacagtccg tcttactttt gaccaactaa aagatttaag taattataaa
     4381 gcaactgcta ataatcgttt cattaatgat ttagaaatga cttatgataa attaatggat
     4441 ttacgttttg gtaaaagaag tgctaatggg ctcgatatag atagattcgt tatgtttagt
     4501 gagtttaaaa tacgtggaac tgctaaagat ccatatgtag acatacaagt ttacgaaaaa
     4561 gctttacctt tactaaacaa tttagatgag tgggttcgct attcactcca acaatttaac
     4621 gaattacaaa gtagctatgc aaagactatg tttcgattac taaaacaata tcgaactaaa
     4681 ggttttgctt atttttctaa agaggatttt catgaactat tagatattcc aaaaagttac
     4741 tggaacaacc aagcaaacgt tgataatcgt gttataaagc ctatacgaca ggaattaacc
     4801 gcaatcttca aaggattatc aatcaaaaag aaatacggaa aagggcgagg aaaacctgtc
     4861 gttggctatc aatttacgtt taaaccagaa atcaaaaaag cagatgattt taataaacat
     4921 gcaccaacca aagaagaaca aaataaagca ttggctttag ctgataaaca aaaagaatac
     4981 gaagaacctg ctacaacaat taacgaaaaa atcaaacaga agaaacgaga acttgaactc
     5041 ctccaacaaa tcaaacaact agaaaaagag aaagaagctc ttgaaaaaca aacagagtcc
     5101 gcactttcga aaaaagagaa aaaaagttta ttatctgatt tagataattt attccagtag
     5161 caacctagga ggcaaccagt atgcaaccac aagaaacctt tacaataaag gaattgtcag
     5221 aactttttaa gatgagtagg caagcaatat cgaaacatat ccaaaaatta gattcttcca
     5281 tgatagctaa aaatgagcgt ggttataagg ttgtattgcg ttctggagta ttgcaacttg
     5341 ctcgcaactt aggcaaccaa atgttgctag ataggttgca cgagtacgaa caagacaacg
     5401 ataaaaatgt taatatacca agcaaaaacg tggttacaaa caagttgcta gatcagttgc
     5461 gtgaagaaaa tgaattttta cgagaacaac ttgccctcag agagcaaaat aattctcata
     5521 tacaaaaact actcgaccag caacaacaac tcaccctaca agccaacaaa caaatcgaaa
     5581 aactgcaaga aaaactacag ttgacttata cagaagaagc tccagagaat gactcaacga
     5641 ctctttctga agcaacagaa acaatagaca aacaaattga tactgaaaaa aaatggtggc
     5701 aactttggaa ataactggag ggaacattca tgacaagaaa aaatcttcta ttcggttcta
     5761 ttttactagg aattctagga ttcagtccga tgttattgtc agtgatcaat aaagggaata
     5821 aacatggtga attgaaattg aatccaaatt attacaacag tcctaacgaa gaattgtttg
     5881 taaaagaaac ggataaataa taaaaaagac tgctctttcg agcagccagt taaaaaataa
     5941 tgagtgttta ttcttgaaaa agcaacgcgc ttatgaagcg ctaccaaaaa tttatcatat
     6001 tttagtgaac tactcaccac ttagctaaac ctgacggttc ttaacgcttg aagtgggagc
     6061 ttcttgggaa tagagcatac ttgatagcgt atttctttac taacagcggt gtctcttatt
     6121 taccaagcta tccccgtagt tcctacggtt cttgatgatt ggatctaagc caatcccata
     6181 gaatgtagtc ttagaccttc ggtcagaata ttgatactag cattgatatc acgatcatga
     6241 tgagtatgac aaacaggaca agtccattct cgaatttcaa gagatttctt gccatcttta
     6301 tgtccgcact ctgaacaaat ctgactagac ggaaaccatt tatctacttt gatgattttt
     6361 cgtccatacc agtcagcctt gtattgtaat ttagtcacaa actttgacca agaaacatca
     6421 gaaatacttt tagctaattt atgatttcgc aacatacctt ttgtgtttaa gtcttcaata
     6481 cagataatat cgtgattttt gatcatttct gtactcaact tattcagaaa atcagtacgt
     6541 tgattcatta ctttttcatg caatctagct actttgcgtt tttgtttttg ataattttta
     6601 gcttcaaata gattggtacc tttattttta gccaataaag cacgcctaga caatttgcgt
     6661 tgttcacgct ttagtttctt ttccattttg gacgtgaatt tattattatc aattttttgt
     6721 ccatcagaaa gaatcgcaaa gtccataata cctaagtcaa taccaattgt ggaattagtt
     6781 ttaggtaatt cgataatttc ttctttacac aacaaagaaa tatagtattt accgctagaa
     6841 tgacgtgata ttgtagcgga tttgataatc ccttttggtt gtctatgaag cttaattctt
     6901 actaatgact tcaatttagg aactttgatg aatttactat caatcaaagc aactgtacca
     6961 ttttgattat tcgttgtata actctgaaca ggatttttct tacttttgaa acgtggaaat
     7021 ccaacggatt tatcccgaaa aaaattcttg tatgctttat ctaaattaag ttgggcattg
     7081 gctaaagcaa ggctatcaat ttctttcaaa aacggaaatt ctttcttgta tttagctggt
     7141 gtcggaaaag tcattttttt agaagaatca tttttaactt cttcatacgc cttttttcgg
     7201 tcatcaagca ttagattgta gaccttacgg acacaaccaa aagatttggc aaagaagatt
     7261 tcttgttcct ctgttggata gattctgaat ttgtatgctt ttagttgttc cataaagttc
     7321 acctactttc tatttatgat tgtttccttg attttggata tacttcttaa tcacatcaat
     7381 tggtgcgcca ccagttgtca gaagacaaaa acttttagac cagaacattt ctttccaaag
     7441 aaattgtttg actcttggaa agtcacgttt tatcaatctt gagctggcac ttttataggc
     7501 attgatgaat tttgtcaatt ctgttttagg ttgagctttg aacataatat gaacgtggtc
     7561 tttatcatga ttccactcca ctaaagtaat gtgatacgat tctgaaattc tttcaaaagt
     7621 agttttggca taatcagata tttcatcatc aatcacttgt ctacgatatt tcgttactaa
     7681 aacaaggtgg taataaagaa gaaatactga atgattattt gtatctaatt ccatgttata
     7741 tcaactcatt tcttgtatag actgattgta acatggaaca aaataaaaag gcaatacgcc
     7801 ttagttgtcg gtcttcgagt taccaacggt aaccctcata ccgaacggca ttcatcaccc
     7861 acctatagag gatgggcgac ttctgcctaa ttctgttaaa aataacaacc cagtaaaaaa
     7921 tgactggtaa aaattcacaa agaaacggaa agaagcaacc gcagaaaatc aagcttaaaa
     7981 agagcttttg acctagacca atcgaaaatc acaaagtgag cctctgtatc ggttgatcca
     8041 ggggctactt ggtagtatgt acattatgtc aacaagaatt taatataaca tattttccta
     8101 atgcattcta actataccac attatcaaaa atataaatta tctttactaa ataaaatatt
     8161 gacaagccta taccttttgt tttatgattt tcac
//
