LOCUS       NZ_CP066425            12538 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 55 plasmid p55_12kb, complete
            sequence.
ACCESSION   NZ_CP066425
VERSION     NZ_CP066425.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038079
            Assembly: GCF_016406385.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 112.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/19/2024 03:51:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,148
            CDSs (total)                      :: 3,056
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 43 of 133
            Pseudo Genes (incomplete)         :: 77 of 133
            Pseudo Genes (internal stop)      :: 36 of 133
            Pseudo Genes (multiple problems)  :: 19 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066425.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 55"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p55_12kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            1..933
                     /locus_tag="JCP69_RS15495"
                     /old_locus_tag="JCP69_15495"
     CDS             1..933
                     /locus_tag="JCP69_RS15495"
                     /old_locus_tag="JCP69_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="JCP69_RS15500"
                     /old_locus_tag="JCP69_15500"
     CDS             950..1420
                     /locus_tag="JCP69_RS15500"
                     /old_locus_tag="JCP69_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1821
                     /locus_tag="JCP69_RS15505"
                     /old_locus_tag="JCP69_15505"
     CDS             1504..1821
                     /locus_tag="JCP69_RS15505"
                     /old_locus_tag="JCP69_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2111..3292
                     /locus_tag="JCP69_RS15510"
                     /old_locus_tag="JCP69_15510"
     CDS             2111..3292
                     /locus_tag="JCP69_RS15510"
                     /old_locus_tag="JCP69_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(3521..4669)
                     /gene="tnpB"
                     /locus_tag="JCP69_RS15515"
                     /old_locus_tag="JCP69_15515"
     CDS             complement(3521..4669)
                     /gene="tnpB"
                     /locus_tag="JCP69_RS15515"
                     /old_locus_tag="JCP69_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4686..5090)
                     /gene="tnpA"
                     /locus_tag="JCP69_RS15520"
                     /old_locus_tag="JCP69_15520"
     CDS             complement(4686..5090)
                     /gene="tnpA"
                     /locus_tag="JCP69_RS15520"
                     /old_locus_tag="JCP69_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            5728..6540
                     /locus_tag="JCP69_RS15525"
                     /old_locus_tag="JCP69_15525"
     CDS             5728..6540
                     /locus_tag="JCP69_RS15525"
                     /old_locus_tag="JCP69_15525"
                     /inference="COORDINATES: protein motif:HMM:NF026081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="WP_233098637.1"
                     /translation="MKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQ
                     AINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNT
                     IKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLW
                     KSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWR
                     IAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNVNYSPLS"
                     /name="cysteine protease staphopain"
     gene            complement(6677..7825)
                     /gene="tnpB"
                     /locus_tag="JCP69_RS15530"
                     /old_locus_tag="JCP69_15530"
     CDS             complement(6677..7825)
                     /gene="tnpB"
                     /locus_tag="JCP69_RS15530"
                     /old_locus_tag="JCP69_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(7842..8246)
                     /gene="tnpA"
                     /locus_tag="JCP69_RS15535"
                     /old_locus_tag="JCP69_15535"
     CDS             complement(7842..8246)
                     /gene="tnpA"
                     /locus_tag="JCP69_RS15535"
                     /old_locus_tag="JCP69_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            8558..8764
                     /locus_tag="JCP69_RS15770"
     CDS             8558..8764
                     /locus_tag="JCP69_RS15770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033794861.1"
                     /translation="MGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISA
                     NAGPVTIPVPGGGFTWDNTITNW"
                     /name="hypothetical protein"
     gene            8737..9180
                     /locus_tag="JCP69_RS15545"
                     /old_locus_tag="JCP69_15545"
     CDS             8737..9180
                     /locus_tag="JCP69_RS15545"
                     /old_locus_tag="JCP69_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            9482..9862
                     /locus_tag="JCP69_RS15550"
                     /old_locus_tag="JCP69_15550"
     CDS             9482..9862
                     /locus_tag="JCP69_RS15550"
                     /old_locus_tag="JCP69_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002348702.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9844..10758
                     /locus_tag="JCP69_RS15555"
                     /old_locus_tag="JCP69_15555"
     CDS             9844..10758
                     /locus_tag="JCP69_RS15555"
                     /old_locus_tag="JCP69_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002348701.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10718..10996
                     /locus_tag="JCP69_RS15950"
     CDS             10718..10996
                     /locus_tag="JCP69_RS15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            10998..11687
                     /locus_tag="JCP69_RS15560"
                     /old_locus_tag="JCP69_15560"
     CDS             10998..11687
                     /locus_tag="JCP69_RS15560"
                     /old_locus_tag="JCP69_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9845..10755
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatt atggtaaaaa atcatacaaa
     1741 aacactaata ctattatttc attttgtaat ttttttaagt ttagttttca ccttaattca
     1801 gtccggattc ttttatttta gccattcaat tgccacttgg ttattaatat tttttctaag
     1861 tttactatta ataattaata cattaataat taatacttta attattatct ttaataaaca
     1921 tagaaactta tttcttcaca gcatatttcc aattgtttta ttaatttttc tatactttat
     1981 ataaaaagat agaactattt agtaagataa aaaagtagct aattgtaatt ttcgtctaaa
     2041 actcaaaact agtaaaaata actttattgt gttattttta tttataaatt atgaaaaagg
     2101 acctttcgaa atgaaaaata atagaatcta caaaaatcag ttgaattatg ggcttttatt
     2161 gccagttttt ctgctatgta tcattggatt actttcttta tatgttgctt tatatcacga
     2221 ctcttacact aattcaaaaa tcacaaaaga attaattaaa caagtattgt ggtatagtat
     2281 tgggttcact tcagcaataa ctatttattt ttttaatcct aaaattttgt ggaaattaac
     2341 tcctatattt tacggattat gcatcataat gttattactt ctcttgaaat tctataatcc
     2401 acaattagca gtcttaactg gagcaaaaaa ttggttttat attgctggtt tttcgtttca
     2461 acctactgag ttagtcaaaa taggatacac ccttatgcta gctaaaataa ccgtcaatta
     2521 tcagcaaaaa tctattacta gtaatacgaa agatgattgg atttatttac ttaaaacttt
     2581 tattgctact cttccaatag gaatattatt attttttcag aacgatctag gaacaatgtt
     2641 agtcttcatt gttatttata tttttatttt gtttacgtct aacataaata taaaaataat
     2701 tgccccaata atcgccattg gtgttgtttt atccttaata tttttatatt tggttatatc
     2761 agatacagga agagatttct tattaaaaat aggatttcac gactatcagt ttaaaagaat
     2821 agactcatgg ttgaatccat ttttagatcc aaatggatct agctatcagt tagcaaatag
     2881 tttaattgct attggctctg gcggactttt aggtactggt tttaatgtat caaacattca
     2941 cgttcctgtt agagaatccg atatgatatt ttctgtaatt ggagaaaatt ttggctttat
     3001 aggaagttgt cttgtaatat tcctatactt ttatttaatt tatcaaatgt ataaaacatg
     3061 ttttaaaagc aataacttat ttttcaccta tatcggtata ggcattgttg caatgatttt
     3121 ttttcatgtt tttgaaaata taggtgcaag tatcggatta ctacctctaa ctggtattcc
     3181 actcccctat attagtcaag gtggttctgc cattcttagt aattttttgg ggttgggatt
     3241 tatgttgtgt tgtagtagat atactaacga aaaaaaagga acacaaaaat gaattcaaaa
     3301 taacacttta tgaatagaat gtctatttca ataaaaatta tgatacctta aagttaatca
     3361 gtgaactact caccacttag ctaaacctga cggttcttaa cgcttgaagt gggagcttct
     3421 tgggaataga gcatacttga tagcgtattt ctttactaac agcggtgtct cttatttacc
     3481 aagctatccc cgtagttcct acggttcttg atgattggat ctaagccaat cccatagaat
     3541 gtagtcttag accttcggtc agaatattga tactagcatt gatatcacga tcatgatgag
     3601 tatgacaaac aggacaagtc cattctcgaa tttcaagaga tttcttgcca tctttatgtc
     3661 cgcactctga acaaatctga ctagacggaa accatttatc tactttgatg atttttcgtc
     3721 cataccagtc agccttgtat tgtaatttag tcacaaactt tgaccaagaa acatcagaaa
     3781 tacttttagc taatttatga tttcgcaaca taccttttgt gtttaagtct tcaatacaga
     3841 taatatcgtg atttttgatc atttctgtac tcaacttatt cagaaaatca gtacgttgat
     3901 tcattacttt ttcatgcaat ctagctactt tgcgtttttg tttttgataa tttttagctt
     3961 caaatagatt ggtaccttta tttttagcca ataaagcacg cctagacaat ttgcgttgtt
     4021 cacgctttag tttcttttcc attttggacg tgaatttatt attatcaatt ttttgtccat
     4081 cagaaagaat cgcaaagtcc ataataccta agtcaatacc aattgtggaa ttagttttag
     4141 gtaattcgat aatttcttct ttacacaaca aagaaatata gtatttaccg ctagaatgac
     4201 gtgatattgt agcggatttg ataatccctt ttggttgtct atgaagctta attcttacta
     4261 atgacttcaa tttaggaact ttgatgaatt tactatcaat caaagcaact gtaccatttt
     4321 gattattcgt tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa
     4381 cggatttatc ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta
     4441 aagcaaggct atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg
     4501 gaaaagtcat ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat
     4561 caagcattag attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt
     4621 gttcctctgt tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct
     4681 actttctatt tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt
     4741 gcgccaccag ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat
     4801 tgtttgactc ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg
     4861 atgaattttg tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta
     4921 tcatgattcc actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt
     4981 ttggcataat cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca
     5041 aggtggtaat aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa
     5101 ctcatttctt gtatagactg attgtaacat ggaacaaaat aaaaaggcaa tacgccttag
     5161 ttgtcggtct tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct
     5221 atagaggatg ggcgacttct gcctaattct gttaaattca aattattaca acagtcctaa
     5281 cgaagaattg tttgtaaaac aaacggataa ataataaaaa agactgctct ttcgagcagc
     5341 cagttaaaaa ataatgagtg tttattcttg aaaaagcaac gcgcttatga agcgctacca
     5401 aaaatttatc atattttatg aaaaataaca ccccagtaaa aaatgactgg taaaaattca
     5461 caaagaaacg gaaagaagca actgcagaaa atcaagcctt aaaaagagct tttgacctag
     5521 accaatgaaa aatcacaaag tgagcccctg tatcccttgg tagagaggct acttggcata
     5581 gtcgacatta tgttaactaa cgctataaat aacgattatg ttcccaaaac aatattttat
     5641 gaactataac aaaaatttgt tatcgcatat tgtatatttt aattataggt gtataccact
     5701 taaatttaac acaaaggcgt gattattttg aaacccaaaa agaaaattta tctaggtctc
     5761 ctcttttcct tgatgattgg aatttttagt ataggaatta caaataccta tgcttcagaa
     5821 aattctaatt tcaatatcaa ctcccaacca attgaagaac aagcaattaa ttttgcgaaa
     5881 actgattttg aacactccat aacttcaatt cttttaatgg aaaataacac cgaaactgat
     5941 attaactata ctcttggttc gccatttaaa atgctcgatg gagaaaatac ggcaaatagt
     6001 tctattttct ttccagtatt ttcagacgct gataatacga ttaaatatat ttataccgtc
     6061 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
     6121 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
     6181 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
     6241 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
     6301 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
     6361 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
     6421 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
     6481 tcagctagag ccattatgca ttatacctat cctaatgtga actactcacc acttagctaa
     6541 acctgacggt tcttaacgct tgaagtggga gcttcttggg aatagagcat acttgatagc
     6601 gtatttcttt actaacagcg gtgtctctta tttaccaagc tatccccgta gttcctacgg
     6661 ttcttgatga ttggatctaa gccaatccca tagaatgtag tcttagacct tcggtcagaa
     6721 tattgatact agcattgata tcacgatcat gatgagtatg acaaacagga caagtccatt
     6781 ctcgaatttc aagagatttc ttgccatctt tatgtccgca ctctgaacaa atctgactag
     6841 acggaaacca tttatctact ttgatgattt ttcgtccata ccagtcagcc ttgtattgta
     6901 atttagtcac aaactttgac caagaaacat cagaaatact tttagctaat ttatgatttc
     6961 gcaacatacc ttttgtgttt aagtcttcaa tacagataat atcgtgattt ttgatcattt
     7021 ctgtactcaa cttattcaga aaatcagtac gttgattcat tactttttca tgcaatctag
     7081 ctactttgcg tttttgtttt tgataatttt tagcttcaaa tagattggta cctttatttt
     7141 tagccaataa agcacgccta gacaatttgc gttgttcacg ctttagtttc ttttccattt
     7201 tggacgtgaa tttattatta tcaatttttt gtccatcaga aagaatcgca aagtccataa
     7261 tacctaagtc aataccaatt gtggaattag ttttaggtaa ttcgataatt tcttctttac
     7321 acaacaaaga aatatagtat ttaccgctag aatgacgtga tattgtagcg gatttgataa
     7381 tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta ggaactttga
     7441 tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta taactctgaa
     7501 caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga aaaaaattct
     7561 tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca atttctttca
     7621 aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt ttagaagaat
     7681 catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg tagaccttac
     7741 ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga tagattctga
     7801 atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg attgtttcct
     7861 tgattttgga tatacttctt aatcacatca attggtgcgc caccagttgt cagaagacaa
     7921 aaacttttag accagaacat ttctttccaa agaaattgtt tgactcttgg aaagtcacgt
     7981 tttatcaatc ttgagctggc acttttatag gcattgatga attttgtcaa ttctgtttta
     8041 ggttgagctt tgaacataat atgaacgtgg tctttatcat gattccactc cactaaagta
     8101 atgtgatacg attctgaaat tctttcaaaa gtagttttgg cataatcaga tatttcatca
     8161 tcaatcactt gtctacgata tttcgttact aaaacaaggt ggtaataaag aagaaatact
     8221 gaatgattat ttgtatctaa ttccatgtta tatcaactca tttcttgtat agactgattg
     8281 taacatggaa caaaataaaa aggcaatacg ccttagttgt cggtcttcga gttaccaacg
     8341 gtaaccctca taccgaacgg cattcatcac ccacctatag aggatgggcg acttctgcct
     8401 aattctgtta aacaacagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa
     8461 atatgcaaat tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga
     8521 ggtccaaaca caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa
     8581 ttatagtaac agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca
     8641 aacttactat gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg
     8701 accagttaca attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg
     8761 gtaaataaac ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt
     8821 tcaaaaaaac aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact
     8881 aaagatcatt cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa
     8941 tataccatct tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat
     9001 ataaatgaac aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat
     9061 aaaaaacaat tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg
     9121 acaacgaatg atgggacaga agggacagct aaaccaattg aattttttaa agctgattga
     9181 tgaatagttg tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt
     9241 tacaaaaagt ggctagcatt tgctagcctt ttttgtattg tggagcgtgg gttttgaggg
     9301 ggattcaagg gggagcttgc gcccctttga aaagcggtta tttgcaccaa atacgtaggt
     9361 atttggctag caaatataga gcttgccaag cccttttggt gagctttact cattgtgcct
     9421 ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa gggtgggact
     9481 tatgagcgaa caaaatcaaa atttggctag cgatcattct aaaaaatata cctatcgatc
     9541 caagccaaaa caaatcagtt ttcgagtgag cgaatctgaa tttgcaaagc tgaagcagtc
     9601 agctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac aaaacgcgcg
     9661 tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg atttagccaa
     9721 agctggaggg aatatcaatc aaattgccaa gtggtgcaat acacaccaac atgacgttgc
     9781 tcctggagac gcccaacgtt tatccgaaaa cctaaaaatc atgcaaaagg agctacaaaa
     9841 gatatggcaa cagttaaagt aagtcgaacc acttcctgta gccgagcaat caattacgca
     9901 gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc caaaagtgaa
     9961 atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca cttattgatt
    10021 caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg ggcagagtat
    10081 gcaaaaaaaa tagcccccga tcaccaaaca gctatttaca cacatacaga caaagaccat
    10141 attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa atttcaagct
    10201 catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg tcttgcacat
    10261 aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc tgaaataaaa
    10321 ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat cgatcaaaca
    10381 atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa aacattgggg
    10441 attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa aaagaaaaaa
    10501 gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca tgagctggac
    10561 agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc tcgagctagc
    10621 attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc tgaacaaccg
    10681 gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac agaccaccaa
    10741 caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga aacaacgtgc
    10801 ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac aagttaaaaa
    10861 actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc ttattcaacg
    10921 atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg cattttggtt
    10981 agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt gttagtattt
    11041 caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta aaaggctcta
    11101 aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata atcgtttcaa
    11161 gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt ttattaggaa
    11221 atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa gcattagcac
    11281 aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt gtgaatcaag
    11341 aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa aacttgccag
    11401 aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac gtgcaatatg
    11461 tgaaagatac gaaagtggaa gaagcttgta gtgatttatt tggagataca attccttttt
    11521 caactagcga acaagaacga aaagaacgac aacaatcagt cacatatgaa cgctatgaaa
    11581 tcttgcttca acaagcaatt aacatacaaa atctgactaa aaaagaaaca gctttaaatt
    11641 atttaaaaag cgtcaaggaa tggttagatc agcaacagca aaattgaatt tgattgaaat
    11701 tcatgttaaa ctaaacatgc aaatgttcaa acattagata cacgaaagcc ccagagaggt
    11761 cggattctct ggggcttttt ttaggttgat gtcgccttta ctgattttac ttcttgtttc
    11821 ggttattcaa ccaataactg aatacagaga tacaaactcc tacaagaagg gggcaaataa
    11881 acagttcaaa catcagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt
    11941 tattataccc tacaaaatta aatctgtgtg gcaattttaa gtatctctta tttccgggtt
    12001 gttagggctt gccctgaccg cctgtagggc gctttaaatt ccagataaag ttttttatac
    12061 taagactgtc ttaaaacggc ttagacaagc tcttagagcc tcaaccctcc tttttgaaca
    12121 atggttttag caaaggagct ggaggcgact gcgcaggtat aatgtgagag cgtagcgatc
    12181 acaccttttt ggcgaccgta gggagcgagg ttttgaatta gcagtttaga aaagttcgcg
    12241 aacctgttta aagaaaaaat aattgttttt ttaggtggga aattttgtct ccctctttta
    12301 taatcttttt ttaaacctct ttttaaacct ctttaacgtc ggctgtgtcc ttactccccc
    12361 aaggtataca aaattttata ggcatgaaat tatctgttta taggcatgaa attatctgtt
    12421 tataggcatg aaattatctg tttataggca tgaaattatc tgttttaggt attgttttta
    12481 gacataatat gtcctaaaat gatgtcaata aaatcattta gactggagta acaactaa
//
