LOCUS       NZ_CP066418            11819 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 53_1 plasmid p53_1_11kb, complete
            sequence.
ACCESSION   NZ_CP066418
VERSION     NZ_CP066418.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038078
            Assembly: GCF_016406365.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11819)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 256.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/19/2024 03:59:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,131
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 44 of 122
            Pseudo Genes (incomplete)         :: 72 of 122
            Pseudo Genes (internal stop)      :: 30 of 122
            Pseudo Genes (multiple problems)  :: 20 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066418.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 53_1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p53_1_11kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            1..1041
                     /locus_tag="JCP73_RS15430"
                     /old_locus_tag="JCP73_15420"
     CDS             1..1041
                     /locus_tag="JCP73_RS15430"
                     /old_locus_tag="JCP73_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_000997689.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLLK
                     DRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVN
                     KRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIA
                     KIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKE
                     DSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDA
                     DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVS
                     TENWLE"
                     /name="replication initiator protein A"
     gene            complement(1657..2337)
                     /locus_tag="JCP73_RS15435"
                     /old_locus_tag="JCP73_15425"
     CDS             complement(1657..2337)
                     /locus_tag="JCP73_RS15435"
                     /old_locus_tag="JCP73_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_002287920.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIGADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(2508..3188)
                     /locus_tag="JCP73_RS15440"
                     /old_locus_tag="JCP73_15430"
     CDS             complement(2508..3188)
                     /locus_tag="JCP73_RS15440"
                     /old_locus_tag="JCP73_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_002287920.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIGADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <3264..3830
                     /locus_tag="JCP73_RS15445"
                     /old_locus_tag="JCP73_15435"
                     /pseudo=""
     CDS             <3264..3830
                     /locus_tag="JCP73_RS15445"
                     /old_locus_tag="JCP73_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3877..4113)
                     /locus_tag="JCP73_RS15905"
     CDS             complement(3877..4113)
                     /locus_tag="JCP73_RS15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306297428.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISFF
                     LLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
                     /name="hypothetical protein"
     gene            4211..4390
                     /locus_tag="JCP73_RS15450"
                     /old_locus_tag="JCP73_15440"
     CDS             4211..4390
                     /locus_tag="JCP73_RS15450"
                     /old_locus_tag="JCP73_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001120991.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLLI
                     AQEKAGFEVPALKK"
                     /name="hypothetical protein"
     gene            4521..4790
                     /locus_tag="JCP73_RS15455"
                     /old_locus_tag="JCP73_15445"
     CDS             4521..4790
                     /locus_tag="JCP73_RS15455"
                     /old_locus_tag="JCP73_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_000388479.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRD
                     YDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            4783..5040
                     /locus_tag="JCP73_RS15460"
                     /old_locus_tag="JCP73_15450"
     CDS             4783..5040
                     /locus_tag="JCP73_RS15460"
                     /old_locus_tag="JCP73_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_000588503.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGK
                     PEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            5499..6503
                     /locus_tag="JCP73_RS15465"
                     /old_locus_tag="JCP73_15455"
     CDS             5499..6503
                     /locus_tag="JCP73_RS15465"
                     /old_locus_tag="JCP73_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455809.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINVF
                     FYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACEI
                     GVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKL
                     VHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQITL
                     SKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
                     /name="hypothetical protein"
     gene            complement(6668..7282)
                     /locus_tag="JCP73_RS15470"
                     /old_locus_tag="JCP73_15460"
     CDS             complement(6668..7282)
                     /locus_tag="JCP73_RS15470"
                     /old_locus_tag="JCP73_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001261742.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRPV
                     FQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPL
                     LDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRL
                     VYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
                     /name="recombinase family protein"
     gene            7732..9057
                     /locus_tag="JCP73_RS15475"
                     /old_locus_tag="JCP73_15465"
     CDS             7732..9057
                     /locus_tag="JCP73_RS15475"
                     /old_locus_tag="JCP73_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_000969590.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVG
                     VGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMG
                     IMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
                     /name="Y-family DNA polymerase"
     gene            9050..9400
                     /locus_tag="JCP73_RS15480"
                     /old_locus_tag="JCP73_15470"
     CDS             9050..9400
                     /locus_tag="JCP73_RS15480"
                     /old_locus_tag="JCP73_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000568378.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTEE
                     IAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIRHI
                     EFYQDNKWSRLE"
                     /name="hypothetical protein"
     gene            9397..9576
                     /locus_tag="JCP73_RS15485"
                     /old_locus_tag="JCP73_15475"
     CDS             9397..9576
                     /locus_tag="JCP73_RS15485"
                     /old_locus_tag="JCP73_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001021554.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHNFRFYIFPLYI"
                     /name="hypothetical protein"
     gene            9712..10002
                     /locus_tag="JCP73_RS15490"
                     /old_locus_tag="JCP73_15480"
     CDS             9712..10002
                     /locus_tag="JCP73_RS15490"
                     /old_locus_tag="JCP73_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000026576.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITRQ
                     RRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
                     /name="hypothetical protein"
     gene            10370..11158
                     /locus_tag="JCP73_RS15495"
                     /old_locus_tag="JCP73_15485"
     CDS             10370..11158
                     /locus_tag="JCP73_RS15495"
                     /old_locus_tag="JCP73_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000101106.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQGN
                     ATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVGSE
                     HRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKLSET
                     IETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRTITVD
                     EAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
                     /name="AAA family ATPase"
     gene            11145..11471
                     /locus_tag="JCP73_RS15500"
                     /old_locus_tag="JCP73_15490"
     CDS             11145..11471
                     /locus_tag="JCP73_RS15500"
                     /old_locus_tag="JCP73_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000796719.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKDQ
                     GSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLAKK
                     QNKI"
                     /name="hypothetical protein"
     Typing          2..1041
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgatt ttaattatta caaatcaaaa gaaatttatc gtgaaaaata ttatcaaatg
       61 cctaaggtat tttttactaa cgaaaaatac atggatctat caaatgatgc aaaaattgct
      121 tatatgctac ttaaagatcg atttgattat tcagtcaaaa ataactgggt agattctgat
      181 gataatatat ttttcatatt cacagttgaa gaattaatga aattgttaca atgccgcgaa
      241 ggtaaagtat ctaaaatcaa aaaggaatta gaaaaagcag gtcttctgaa acagaagaga
      301 ggaagagtaa ataaacgtga cggtaagatt gaatccatgc ctaatttatt gtacttaggc
      361 aagccagaag taacgaatca agacgtcttt aaaatcatgg aagaggaaga caatactgat
      421 accaccgtaa ttgcgaaaat cgcaaatacg gcaaaaccgc gagaaatcaa tgataccacc
      481 gtaattgcga aaatcgcaaa tacggcaaaa tccagtcata tcaaggactc aaccgtaatt
      541 gcgaaaatcg cagacaatct attctattct aattctatag acactaatag acacataata
      601 gacactgaaa ctgatcagtt acaaaatcaa gttttactag ataacttcgt cgacatcatg
      661 aaagaggaca gtataaatac atttgtacca gaaaatgtat taaacttgat aaaaacattt
      721 tctagtactt acgaagacgc tcaaaaaacc gtccaaacta ttcataatgc aaagaaaaaa
      781 gctgaagaat tagagcaaac agttgttgtt tatgaagaac tggaatctta tggaattgat
      841 gctgacaaag gattatatat gactttgctg aaagcttatc aaaaacaaaa aactgaaaaa
      901 gtaaacaatt tgcagaatct aattttcgtt tatgtaaaaa attggtttgt cgaaaaagct
      961 attgcggcaa agcttgcaaa cgaacaaaga gaaacattta acgaattacc aacagttagt
     1021 acggaaaatt ggctagagta gttgataaaa agcgatcact ataaaaatga tcaattttaa
     1081 attagaactt ttttatgatt ttaattttat tacaaatgtt acaaataaat agctattatt
     1141 ccggagtttt tagattaaaa gtttatgaaa acaatcacaa acgtaggttt taaagtcata
     1201 atcgttagat attttagtta tttttcattc aaaaaaatag ggaactagtg tttgcatatg
     1261 tgcttttttg agttataata attcatgaag agaggtaatt cacttatttc cccaagctga
     1321 aaagcctgat attacttctt ttcaggcagt gctgctaata ttcaattaca cttttttgta
     1381 tatttttagt tgttttaaat ttaattgtct gatatgcttg tgatgaattg attttcgttc
     1441 tgcacttttg gacgataaat tgtgataatt gaaaatacaa aaaaaagaaa ggcccctgta
     1501 aggctcggga gactggtaat ctccgtcagt gtcctacagg aaagcattaa gatgacgcct
     1561 taatgcgtgc ccttcagtgt tacggtatta cccgcaacgg acggttctgt tgcaaagttt
     1621 taaataaaga ataaaatccc ttacggtatc tatgatttaa gctgggattc ccaataatac
     1681 cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg ttttcttata
     1741 taatcctcga atggcttcca tgcctttaat cgtggtagag gcagtgcgta aacttcgata
     1801 gaatttattg cgtctcttta ctggacgatg gtcttgttca atcaaattat tcaggtattt
     1861 aatggtacga tgttctgtcc cttgataaaa gccgtattct tttagtttct taaaggcact
     1921 tgtaatagag ggggctttat ctgtgactac aaccttcggt tcatcaaact gcttcactaa
     1981 ccgcttaaga aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa
     2041 ggttaaacca tctgcaccga tggctcgata caaataatgc cattttcctt taattttgat
     2101 gtacgtttca tccattttcc atgaataaaa ggatttttta tttttctttt tccaaatttg
     2161 atagagtagt ttgccatatt cttgcaccca acgataaatc gtcgtatgag aaacgttaat
     2221 gccacgatca tataagattt cttgaacttc acgatagcta aggttataac gaagatagta
     2281 gcccacggct acaataatca catcctgctg aaattgcttt cctttaaaat gattcatcgt
     2341 cattcctcct gctatctttt tctattattc taccttattt gatagtagat ttaaaacttt
     2401 gcaacagaac cattttgtgt ataataggaa ttgaagttaa attagatgct aaaggttctg
     2461 ttgcaaagtt ttaaataaag aataaaatcc cttacggtat ctatgattta agctgggatt
     2521 cccaataata ccttgatttc agtacagacc gaaaacccga agagagtgcc ttcttttcgg
     2581 gttttcttat ataatcctcg aatggcttcc atgcctttaa tcgtggtaga ggcagtgcgt
     2641 aaacttcgat agaatttatt gcgtctcttt actggacgat ggtcttgttc aatcaaatta
     2701 ttcaggtatt taatggtacg atgttctgtc ccttgataaa agccgtattc ttttagtttc
     2761 ttaaaggcac ttgtaataga gggggcttta tctgtgacta caaccttcgg ttcatcaaac
     2821 tgcttcacta accgcttaag aaaagcatag gctgcttgtg tgtcccgttt tttacgtaac
     2881 caaatatcca aggttaaacc atctgcaccg atggctcgat acaaataatg ccattttcct
     2941 ttaattttga tgtacgtttc atccattttc catgaataaa aggatttttt atttttcttt
     3001 ttccaaattt gatagagtag tttgccatat tcttgcaccc aacgataaat cgtcgtatga
     3061 gaaacgttaa tgccacgatc atataagatt tcttgaactt cacgatagct aaggttataa
     3121 cgaagatagt agcccacggc tacaataatc acatcctgct gaaattgctt tcctttaaaa
     3181 tgattcatcg tcattcctcc tgctatcttt ttctattatt ctaccttatt tgatagtaga
     3241 tttaaaactt tgcaacagaa cccgaagaac aattgtattt ggaaagtgaa acgagtcaaa
     3301 cgaaggtcac agtcagccat aatcgtttag acgtgtataa acgtcgtcct gaaacggtaa
     3361 aaaaaggaac ggagcttcta cgactagaaa cagaaaaata catcatgaat gtttacctag
     3421 aaaggagtga gctggtcgaa aactttgtca gctacacgat ttgcaggaaa cactattact
     3481 cagaatcaga atattattca atttttgaga gcatggaatt aatggaagaa gaaatggaag
     3541 agctaaacag caaagatgcg acgatagacg attattacct tgtatttact agatttttga
     3601 cgtgtggaac ggaatctatc gtgttaatgg gaatggatta tgaactcgct gtacccgaag
     3661 catttttgag ggaatatgta gaagaagtag ataacccagg cgacgactgg tacacgactt
     3721 taactgattt tttagacacc tatacttacg acgatacaga agaaatccaa aatgaacttg
     3781 accgtaaacg aatgccatat caacgatttg aaaggaaaaa aaggcaataa tcatttatac
     3841 cgaaaagtga gaatgtgtga aataaggaca aaaaacctag taactaaggg aatggttctt
     3901 atttcgcacc aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat aagcgggcaa
     3961 cacccgataa cctaagagaa agaaggagat ccatatgaat aaaatcattc ctctattgtt
     4021 agtcctattt gttggcgaaa tacttatgcg ccatgctgca gctgtgcatt taaacttcgt
     4081 agtatttttg gcaatactgg tattttgttt gatcttttgg cgctttgtat tagcccttgt
     4141 gagtctacta ttcaagatga ttgcagccat atttttcata gggttatttc gatgaaagga
     4201 gagagtacta atgccaattg caataggaaa taaacggtta cccgttacgt tggatgaaaa
     4261 gagacaaaaa gaattgcagc aactaaagca gaagtacggc aaaagtgaat ccaggattat
     4321 gtgtattgcg ttagatttat tgattgccca agaaaaagca ggatttgagg taccagcact
     4381 caaaaagtga cgtcactttt tatcctaaaa actaaaagtg atagcacttt taattataag
     4441 aagttagaat attaatcatt tgcttaattg tacaatataa tgtacaattg ttttatagaa
     4501 ataaataagg ggtgaaagga atggaagcag tagcttattc aaatttccgc caaaatttac
     4561 gtagttatat gaaacaagtt aatgaggatg ctgaaacact tattgtaaca agtaaagatg
     4621 tagaagatac agttgttgta ttatcaaaaa gagattatga ttctatgcaa gaaacgttga
     4681 gaacactttc taataattac gtcatggaaa aaattcgtcg aggagatgaa caattctcca
     4741 aaggtgcatt taaaacacat gacttaatcg aggttgaatc tgatgattaa ggcttggtct
     4801 gatgatgctt gggatgatta tctttattgg catgagcaag gaaacaaaag caatataaaa
     4861 aagattaaca agttaataaa agatatcgat cgttccccct ttgctggatt aggaaaacct
     4921 gagccattaa agcatgattt atctggaaaa tggtccagaa gaattacaga tgaacataga
     4981 ctgatatata gagttgaaaa tgaaacgata tttatttatt ctgcaaaaga tcactattaa
     5041 ccaatcggaa gtaaggaaag ggtcagaaac ttaaaagttt ttgatcctta ttttatttac
     5101 cctagtcatt taaaaagcta atatagctta gtgttgattg ttattaatga atgtgtttgt
     5161 tacgcgtatt acggatataa ggttagtaaa atcatttcta aagttgagga aaagtaaata
     5221 taaatggctt aaatttcaac aatttgaagt tgaatagata tgttataata ctattgtagt
     5281 gtgggatgtt agttactaaa ggatgacgct tatatatatg actgaataga ataagcaata
     5341 ggtttaataa tctattttaa attttttgta ctagttttag ttaattagca aaaacaacaa
     5401 aaataaactt ctcatagaat ttagctaaaa attaatgatt tatttacata ttaaatttgg
     5461 atacagttaa gtaattttta tatattggag gagaagtaat ggaatataaa tttaacttga
     5521 atttgaaaga agtactcccc actcagggaa caataaacat aaatggtcaa gataatgatg
     5581 acgatatagt aataaaaaaa atagaattta ttgattctga tataaatgtt tttttttatt
     5641 ctaaagaaaa attagctgat tttagcaata ttgctgaaat gattgaacgt ttaaaagatt
     5701 ttatgatcag tgtttgggat gtagcgatgc ttgctgacga atcaaattat acttcgagca
     5761 tatacagatt agaagatagt aattggatag aaatttcatt agagttaaag acacttccta
     5821 attccgttaa ctatataatt agagatataa cttctgaagc tcaacaatta gttaattaca
     5881 tgcatggaca cgaaagtatc cccttttctc ttatactcct taaaagaagt aaaaatatat
     5941 caaatactag agataggttt atttcgataa ttacagcctg tgaaataggg gtaaaggaat
     6001 tttatcaaca agagaaacct gatttaactc taatatttga gaatttacaa tctccccctg
     6061 tagacaaatt attagggagc atgtttaaag ggtactttaa tattgagttt ccgaaagaaa
     6121 taagaaagga aattaaaaat ttcatagaaa aacgtaataa attggttcat tctcctcaag
     6181 gaagtaaacc aactatagaa gaatgttttg aatgttatat ggcagtgcta aaaacattta
     6241 atttttttta taaaattaat gatagcttta tgtatagaga tttttatgaa gaatctatag
     6301 aattgactcc aacggaacca aataaatctc aaatcacttt gtcaaaggag ctagagagaa
     6361 aagtagagtc tcaagaacta gctataggaa ttagtataac caatgtgtcg acatacaaag
     6421 aattatacga agctaaatca caagataata aggattttgt agaaaatata gataaggaac
     6481 caacaaatac agtaggggat taacgtaaat aagtgaagat tatattaaca gactctttat
     6541 ttagttaagt aaaaaatggt ttgttggaat tttatgcttt atctgttctg agtaagactt
     6601 aattttatag atagtaatta tatgtaggaa attaaagatg agaagataat aaaagcagca
     6661 tgatcaacta tttgtcattg accatgctgt ctttttttat tctatacacg gtttgtctcg
     6721 taacattgta gtctttagca atcttagcaa tcgcaacacc atttttaaga tcttcaacga
     6781 tgcttttgta aacaaggcga cgttggggat cttttgcgtc tgcactgtat aatttgggcc
     6841 gccctttgta gacgccgttt gcttttgcga tcttaattcc ttgtgcctgg cgacgtttcg
     6901 attcagtccg ttcttgttca gcgatcatgg ccaaaatttg gatgatcaga tccttaatga
     6961 atttatccag gagaggattg ccaattgctt cagccatgat cggtaaacta gtaatgatca
     7021 gcttcacatt tttcttcttc aagtaattga ctgaagcgat gatctcgtca taatttcttc
     7081 caagtcgatc aatagcttcc acaataaaaa tatcttgatc tcggacgtaa tctaatgctt
     7141 cttggaaaac aggtcgatga gtaatcgttg cgccagattg tttctcgatg aaaattctct
     7201 ctgcaccgaa ttttttcatt tcttcaatct ggcgttgttc attttgatcg atcgatgaga
     7261 cacgtatgta tgcaactctc aaaactttta cctactttct gcaactattt aaaatacacc
     7321 ctaatcatac acgaggaagc catatttttc aatgttatac cagtgtctat ttagggtaaa
     7381 ccttatatag acaaaaacta atgatggagg aaatagtatg agaatttttt gtaagtgtct
     7441 actgataact tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg
     7501 gaactccaaa aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt
     7561 ttattagatt ttcaaattaa ttagtaaagt gatagaataa attgatcata caaatcaaaa
     7621 gaagaagcag ggtgatagca tacctatgct tcttttttta tgtatttagc tatttcttct
     7681 ttacaatatc gaacatatgt ttgtataatg tgaaagaatg gagtgttatt tatgatggat
     7741 tattcaaaag agccagtaaa tgattacttt ttaatcgata tgaaaagctt ttacgcaagt
     7801 gtcgaatgta tagaaagaaa tttagatcca ttaacaacag aacttgttgt tatgagtcga
     7861 gctgataata ctggttcagg attgatatta gcttcttctc ctgaagcaaa aaagcggtat
     7921 ggtattacaa atgtgagtag accacgtgac ttaccacaac catttcctaa aacactacac
     7981 gttgttccac cacgtatgaa cctatatatc aagcgaaata tgcaggtaaa taatattttc
     8041 agaagatatg tggctgatga agatctactg atttactcga tcgatgaatc aatcttaaaa
     8101 gtgacccgat cactgaatct ttttacgact gaaggaacac gaagtcaacg gagaaagaag
     8161 ctggctcaaa tgatccagga gaatattaaa gaagagctag gattgattgc tacagtaggt
     8221 gtcggagata atcccctttt ggctaaacta gccttagaca acgaagcaaa gcataatgaa
     8281 ggatttgttg cggaatggac gtacgaaaac gttcctgaaa aagtatggaa tattcctgaa
     8341 atgacggact tttggggaat tggctctcga atgaaaaaaa gactcaatca gatggggatt
     8401 atgagcatta gagatcttgc taactggaat ccttatacga ttaagaatag actaggtgtg
     8461 attggcttac agctgtactt ccatgctaat gggatcgatc gaacagatat agcaatccca
     8521 ccagaaccga caaaggaaaa atcatatgga aattcacaag tgttaccacg agattatact
     8581 agaagaaatg aaatcgagct ggtggtaaaa gaaatggcgg aacaagtagc gatacgcatt
     8641 aggcagcata actgtaaaac aggttgtgtc catttacaca ttggcacgtc gatcttagaa
     8701 acgagaccag ggttttcaca ccagatgaag atccctatca cagataatac aaaggaattg
     8761 cagaattatt gtcttttctt gtttgataag tattatgaag gacaagaagt aaggcatgtt
     8821 ggaataacct atagcaagct tgtgtacacc gattcgcttc agctggattt atttagtgat
     8881 ccacaaaagc aaattgacga agaaaattta gataaaatta ttgacaagat tcggcaaaaa
     8941 tatggtttta cgtcgattgt acatgcttct tcaatgttag aaggcgctag aagcattact
     9001 agaagcacac ttgttggtgg tcatgcgggc ggaaatgggg gaattaaaaa tgattgatta
     9061 caatgatcgt ggacgtttaa agtgggcagg gttttattta tctgatcata ctgaagaaat
     9121 tgatgccgac aatgaacaac gaaccagtca aaatttagca aaagaacaaa tgacgactga
     9181 agagattgcg gaagtattga acgctgcagt cataaaaaat agatcagtat ctattcaaaa
     9241 agaagaacga aacgctgaag gatactatcc agaagatatc attggcaaga tcttaggtag
     9301 tgatgagctg gggatctacg ttggaaaaga caaaattcac tacgacgaaa tacgtcatat
     9361 cgaattttac caggataata aatggagtcg attagaatga acattatcca gcaatatgag
     9421 ctaaagtata taacctttga tcagctatca gaagagattt ggggttatgg ccaacgtttg
     9481 ataaatgagg ttggtgttga gcgcttctct ttctatgtag aagctgcagc aggttatcat
     9541 aattttaggt tttatatctt cccgttatat atataaggta ttttacggat tctatagagt
     9601 tatctctatg aatccttttt tgtttttaca aataggagta gatactagaa taatgcgtgt
     9661 gttaaactat tattgcaaac gcaataataa aagaaagtag gcttacgcac catgtctatc
     9721 aatacatttg tttactcgca cccgatcaat gtttacatta tcaagaattt aggaatcact
     9781 gtagaacaat tttgtgagtt atatgcctac ccacaaggaa cggttgcaag ctggataacc
     9841 cgacaacgta gaataaaatc attacctgct tcattcgttt atgatttaag cctggcctct
     9901 agtttaaaca tgtctgatgt ttatgaaaag ctcctgtctt tagaaaaaga atatgattcc
     9961 ttcaagatta aacatgataa aaaaataaaa aaacatatat aatatcaatc acaattgtat
    10021 aatattacga tatcttaata tcatgatatc gtaatattgt ttttttctaa gaaaatgcac
    10081 tataagaagg gaattattag taacaatata gacagtttag taatgtttaa attattctag
    10141 ttgctgctcg caaaacatat ttcttatcat taataaaaaa ttttaaagta ataaaaagtg
    10201 attattttaa tttcgaataa catatgaata atctttatat ccgaaccgat atatatcttt
    10261 aaaacaaaat aatcacacaa tattacaata taatgcatat acgatattgc aatatcacaa
    10321 tattatgata taattatatt ataatataag atgaatgggg gatattttaa tggcaagaat
    10381 attgtcagta gccaatcaaa aaggtggagt ttctaaaact acgactagcg ttaatttagc
    10441 agcatgtttg aaatttgttt acggtaaaaa tgtactttta gtggacattg atcctcaggg
    10501 aaatgctaca gataatgtag gtttcgatat agatggaaca aaccaaccta caatttatga
    10561 agtattaaaa ggggaggctg acattacaga tgctattctg gactataaag gaattgatgt
    10621 tttgccagcc gacatagccc tttcttctgc agagagggaa tttactcaag taggaagtga
    10681 acacaggctt aaaagagtac ttcaaccaat caaggacaac tatgattata ttataattga
    10741 ttgtccacca agtttaggaa tgcttacagt caacgcattt actgtttctg atgaaattat
    10801 cattccagtt gaagctgcgt atttctcatt aaaaggacta ataaagttaa gtgaaactat
    10861 tgaaacggta aaagaatata cgaacgacaa gcttgaagta cgaggagttc tatttactaa
    10921 gtataataag cgttataaca tttctaaaga aatgacgtct gcagcaaccg aaatttctag
    10981 agtcattggt gcaaaaattt tcaaaactta tattcgtagg acaataaccg ttgacgaagc
    11041 gcaagctgct ggatcagatc taatcaattt taataaaccc tcaacagctg aagatgacta
    11101 taaagcattt acagaagaat atttaaaaga agttggtgat tttgatggca gaagataatc
    11161 gcagtcaagc tgcagtaagt agattactag gaaataaaaa taaaaagaat tttaaatcaa
    11221 aagaagtaga agaaatcgaa ttaaccaaga aaaatataag taaagtcata aaagatcaag
    11281 gaagtagtgt aagaacagca gatagcattc gtataaatcc aattctaatg tcagcactca
    11341 aatattggac gactattgct gaatcagaca aaagtaaacc agacgttata gaagatgcgc
    11401 tattaaagtc catacctgaa gaatacttga ttgaaggata taaactagca aagaaacaaa
    11461 ataaaattta atttacattc aatatcacga tatcataata tcgtgatatt ttttttataa
    11521 aaatcagtag tatcaaagct gataagtcgc ttatcttcat attgtaatat tacgatatcg
    11581 tgatataata ttattgtaat atcgtgatat agttactatg agcttaaacc ttgaaataga
    11641 gcggtttgct ttaatttatt ttttaaggta aactaaaaaa agataaaaaa agactactgc
    11701 tttaacaaag tttggcgact tatcagcagt agaaaaatat atagtggata gatatatttg
    11761 tttaattcat tgtaacatag ttttgaacaa aacgctatcc tacagaaaga ggattggct
//
