LOCUS       NZ_CP066406            29409 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 51_4 plasmid p51_4_29.4kb,
            complete sequence.
ACCESSION   NZ_CP066406
VERSION     NZ_CP066406.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038075
            Assembly: GCF_016406465.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29409)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29409)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 02:56:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,229
            Genes (coding)                    :: 3,102
            CDSs (with protein)               :: 3,102
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 44 of 127
            Pseudo Genes (incomplete)         :: 77 of 127
            Pseudo Genes (internal stop)      :: 35 of 127
            Pseudo Genes (multiple problems)  :: 23 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066406.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..29409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 51_4"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p51_4_29.4kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            1..1041
                     /locus_tag="JCQ06_RS16250"
                     /old_locus_tag="JCQ06_16250"
     CDS             1..1041
                     /locus_tag="JCQ06_RS16250"
                     /old_locus_tag="JCQ06_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_000997689.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLLK
                     DRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVN
                     KRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIA
                     KIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKE
                     DSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDA
                     DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVS
                     TENWLE"
                     /name="replication initiator protein A"
     gene            complement(1657..2337)
                     /locus_tag="JCQ06_RS16255"
                     /old_locus_tag="JCQ06_16255"
     CDS             complement(1657..2337)
                     /locus_tag="JCQ06_RS16255"
                     /old_locus_tag="JCQ06_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <2412..2813
                     /locus_tag="JCQ06_RS16260"
                     /old_locus_tag="JCQ06_16260"
                     /pseudo=""
     CDS             <2412..2813
                     /locus_tag="JCQ06_RS16260"
                     /old_locus_tag="JCQ06_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            3027..3722
                     /gene="vanR-A"
                     /locus_tag="JCQ06_RS16265"
                     /old_locus_tag="JCQ06_16265"
     ARG             3028..3719
                     /name="vanR-A"
                     /gene="vanR-A"
                     /gene_name="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3700..4854
                     /locus_tag="JCQ06_RS16270"
                     /old_locus_tag="JCQ06_16270"
     CDS             3700..4854
                     /locus_tag="JCQ06_RS16270"
                     /old_locus_tag="JCQ06_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="WP_002305818.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKL
                     GDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFD
                     EINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLA
                     HDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQT
                     ITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKN
                     AAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAG
                     LGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
                     /name="vancomycin resistance histidine kinase VanS-A"
     gene            complement(4952..6244)
                     /locus_tag="JCQ06_RS16275"
                     /old_locus_tag="JCQ06_16275"
     CDS             complement(4952..6244)
                     /locus_tag="JCQ06_RS16275"
                     /old_locus_tag="JCQ06_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="WP_000122610.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIYT
                     AQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADTSV
                     AKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTPLPK
                     VLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREAVEWI
                     VIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLG
                     KQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFL
                     YAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHI
                     KVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
                     /name="ISL3-like element IS1251 family transposase"
     gene            6576..7544
                     /locus_tag="JCQ06_RS16280"
                     /old_locus_tag="JCQ06_16280"
     CDS             6576..7544
                     /locus_tag="JCQ06_RS16280"
                     /old_locus_tag="JCQ06_16280"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="WP_001059542.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKSA
                     PFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSP
                     DSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIE
                     RLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQ
                     GAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKL
                     QRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
                     /name="D-lactate dehydrogenase VanH-A"
     ARG             7520..8568
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="glycopeptide resistance gene cluster; Van
                     ligase"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.71"
                     /coverage="101.75"
     ARG             7538..8565
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="D-alanine--(R)-lactate ligase VanA"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.71"
                     /coverage="100.0"
     gene            8574..9182
                     /locus_tag="JCQ06_RS16290"
                     /old_locus_tag="JCQ06_16290"
     CDS             8574..9182
                     /locus_tag="JCQ06_RS16290"
                     /old_locus_tag="JCQ06_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="WP_000402348.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTYE
                     LAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRTEM
                     ISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEAQNR
                     RRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
                     /name="D-Ala-D-Ala dipeptidase VanX-A"
     gene            9610..10521
                     /gene="vanY"
                     /locus_tag="JCQ06_RS16295"
                     /old_locus_tag="JCQ06_16295"
     CDS             9610..10521
                     /gene="vanY"
                     /locus_tag="JCQ06_RS16295"
                     /old_locus_tag="JCQ06_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="WP_000732308.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLE
                     NSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDS
                     NIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEH
                     NSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIRYVGL
                     PHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEIS
                     GNNIDGVIVTVFPGSTHTNSRR"
                     /name="vanY"
     gene            10674..11159
                     /gene="vanZ-A"
                     /locus_tag="JCQ06_RS16300"
                     /old_locus_tag="JCQ06_16300"
     ARG             10675..11156
                     /name="vanZ-A"
                     /gene="vanZ-A"
                     /gene_name="glycopeptide resistance protein VanZ-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(11446..11775)
                     /locus_tag="JCQ06_RS16305"
                     /old_locus_tag="JCQ06_16305"
     CDS             complement(11446..11775)
                     /locus_tag="JCQ06_RS16305"
                     /old_locus_tag="JCQ06_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000754864.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIKI
                     FYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILS
                     AIRVF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            12080..12409
                     /locus_tag="JCQ06_RS16310"
                     /old_locus_tag="JCQ06_16310"
     CDS             12080..12409
                     /locus_tag="JCQ06_RS16310"
                     /old_locus_tag="JCQ06_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000629052.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEPK
                     KEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEELA
                     AEIGL"
                     /name="hypothetical protein"
     gene            12399..12686
                     /locus_tag="JCQ06_RS16315"
                     /old_locus_tag="JCQ06_16315"
     CDS             12399..12686
                     /locus_tag="JCQ06_RS16315"
                     /old_locus_tag="JCQ06_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000312842.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQK
                     TGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(12965..13528)
                     /locus_tag="JCQ06_RS16320"
                     /old_locus_tag="JCQ06_16320"
     CDS             complement(12965..13528)
                     /locus_tag="JCQ06_RS16320"
                     /old_locus_tag="JCQ06_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000017403.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAVR
                     PQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTPHG
                     RLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQVYS
                     VKEITEMTGISKATLYRYLKDNRE"
                     /name="recombinase family protein"
     gene            14171..15124
                     /locus_tag="JCQ06_RS16325"
                     /old_locus_tag="JCQ06_16325"
     CDS             14171..15124
                     /locus_tag="JCQ06_RS16325"
                     /old_locus_tag="JCQ06_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222572.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNFL
                     KCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCS
                     VSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHL
                     EGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITF
                     DCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMEFNQVNQGF
                     ISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
                     /name="IS30 family transposase"
     gene            15102..16577
                     /locus_tag="JCQ06_RS16330"
                     /old_locus_tag="JCQ06_16330"
     CDS             15102..16577
                     /locus_tag="JCQ06_RS16330"
                     /old_locus_tag="JCQ06_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="WP_000443455.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIINK
                     DGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGS
                     KRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKN
                     DENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLEN
                     LKDYKVLRYRDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKK
                     DKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFN
                     KERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMII
                     NKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKK
                     SFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
                     /name="RNA-directed DNA polymerase"
     gene            16915..>18378
                     /locus_tag="JCQ06_RS16335"
                     /old_locus_tag="JCQ06_16335"
                     /pseudo=""
     CDS             16915..>18378
                     /locus_tag="JCQ06_RS16335"
                     /old_locus_tag="JCQ06_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /GO_function="GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA]; GO:0009007 -
                     site-specific DNA-methyltransferase (adenine-specific)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA];
                     GO:0009307 - DNA restriction-modification system [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /name="type I restriction-modification system subunit M"
     gene            complement(18416..19096)
                     /locus_tag="JCQ06_RS16340"
                     /old_locus_tag="JCQ06_16340"
     CDS             complement(18416..19096)
                     /locus_tag="JCQ06_RS16340"
                     /old_locus_tag="JCQ06_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <19172..19738
                     /locus_tag="JCQ06_RS16345"
                     /old_locus_tag="JCQ06_16345"
                     /pseudo=""
     CDS             <19172..19738
                     /locus_tag="JCQ06_RS16345"
                     /old_locus_tag="JCQ06_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(19785..20021)
                     /locus_tag="JCQ06_RS16830"
     CDS             complement(19785..20021)
                     /locus_tag="JCQ06_RS16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306297428.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISFF
                     LLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
                     /name="hypothetical protein"
     gene            20119..20298
                     /locus_tag="JCQ06_RS16350"
                     /old_locus_tag="JCQ06_16350"
     CDS             20119..20298
                     /locus_tag="JCQ06_RS16350"
                     /old_locus_tag="JCQ06_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001120991.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLLI
                     AQEKAGFEVPALKK"
                     /name="hypothetical protein"
     gene            20429..20698
                     /locus_tag="JCQ06_RS16355"
                     /old_locus_tag="JCQ06_16355"
     CDS             20429..20698
                     /locus_tag="JCQ06_RS16355"
                     /old_locus_tag="JCQ06_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_000388479.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRD
                     YDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            20691..20948
                     /locus_tag="JCQ06_RS16360"
                     /old_locus_tag="JCQ06_16360"
     CDS             20691..20948
                     /locus_tag="JCQ06_RS16360"
                     /old_locus_tag="JCQ06_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_000588503.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGK
                     PEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            21586..22194
                     /locus_tag="JCQ06_RS16365"
                     /old_locus_tag="JCQ06_16365"
     CDS             21586..22194
                     /locus_tag="JCQ06_RS16365"
                     /old_locus_tag="JCQ06_16365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224752223.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELKT
                     LPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACEIG
                     VKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKLV
                     HSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
                     /name="hypothetical protein"
     gene            22328..23647
                     /locus_tag="JCQ06_RS16370"
                     /old_locus_tag="JCQ06_16370"
     CDS             22328..23647
                     /locus_tag="JCQ06_RS16370"
                     /old_locus_tag="JCQ06_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="WP_000202380.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSEL
                     AKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKELAS
                     QSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQTNY
                     NTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPNTDIL
                     VRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREVQYFST
                     TYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGTMENFIK
                     EAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHSIRLKFLK
                     VAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
                     /name="IS1380-like element IS1678 family transposase"
     gene            <23851..24093
                     /locus_tag="JCQ06_RS16375"
                     /old_locus_tag="JCQ06_16375"
                     /pseudo=""
     CDS             <23851..24093
                     /locus_tag="JCQ06_RS16375"
                     /old_locus_tag="JCQ06_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(24258..24872)
                     /locus_tag="JCQ06_RS16380"
                     /old_locus_tag="JCQ06_16380"
     CDS             complement(24258..24872)
                     /locus_tag="JCQ06_RS16380"
                     /old_locus_tag="JCQ06_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001261742.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRPV
                     FQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPL
                     LDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRL
                     VYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
                     /name="recombinase family protein"
     gene            25322..26647
                     /locus_tag="JCQ06_RS16385"
                     /old_locus_tag="JCQ06_16385"
     CDS             25322..26647
                     /locus_tag="JCQ06_RS16385"
                     /old_locus_tag="JCQ06_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_000969590.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVG
                     VGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMG
                     IMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
                     /name="Y-family DNA polymerase"
     gene            26640..26990
                     /locus_tag="JCQ06_RS16390"
                     /old_locus_tag="JCQ06_16390"
     CDS             26640..26990
                     /locus_tag="JCQ06_RS16390"
                     /old_locus_tag="JCQ06_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000568378.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTEE
                     IAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIRHI
                     EFYQDNKWSRLE"
                     /name="hypothetical protein"
     gene            26987..27166
                     /locus_tag="JCQ06_RS16395"
                     /old_locus_tag="JCQ06_16395"
     CDS             26987..27166
                     /locus_tag="JCQ06_RS16395"
                     /old_locus_tag="JCQ06_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001021554.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHNFRFYIFPLYI"
                     /name="hypothetical protein"
     gene            27302..27592
                     /locus_tag="JCQ06_RS16400"
                     /old_locus_tag="JCQ06_16400"
     CDS             27302..27592
                     /locus_tag="JCQ06_RS16400"
                     /old_locus_tag="JCQ06_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000026576.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITRQ
                     RRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
                     /name="hypothetical protein"
     gene            27960..28748
                     /locus_tag="JCQ06_RS16405"
                     /old_locus_tag="JCQ06_16405"
     CDS             27960..28748
                     /locus_tag="JCQ06_RS16405"
                     /old_locus_tag="JCQ06_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000101106.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQGN
                     ATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVGSE
                     HRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKLSET
                     IETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRTITVD
                     EAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
                     /name="AAA family ATPase"
     gene            28735..29061
                     /locus_tag="JCQ06_RS16410"
                     /old_locus_tag="JCQ06_16410"
     CDS             28735..29061
                     /locus_tag="JCQ06_RS16410"
                     /old_locus_tag="JCQ06_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000796719.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKDQ
                     GSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLAKK
                     QNKI"
                     /name="hypothetical protein"
     ARG             6577..7541
                     /name="vanH-A"
                     /gene="vanH-A"
                     /gene_name="D-lactate dehydrogenase VanH-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8575..9179
                     /name="vanX-A"
                     /gene="vanX-A"
                     /gene_name="D-Ala-D-Ala dipeptidase VanX-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3701..4851
                     /name="vanS-A"
                     /gene="vanS-A"
                     /gene_name="vancomycin resistance histidine kinase VanS-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9611..10518
                     /name="vanY-A"
                     /gene="vanY-A"
                     /gene_name="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             6577..7544
                     /name="vanH gene in vanA cluster"
                     /gene="vanH gene in vanA cluster"
                     /gene_name="vanH; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3028..3722
                     /name="vanR gene in vanA cluster"
                     /gene="vanR gene in vanA cluster"
                     /gene_name="glycopeptide resistance gene cluster; vanR"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9611..10521
                     /name="vanY gene in vanA cluster"
                     /gene="vanY gene in vanA cluster"
                     /gene_name="vanY; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             10675..11159
                     /name="vanZ gene in vanA cluster"
                     /gene="vanZ gene in vanA cluster"
                     /gene_name="vanZ; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3701..4855
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="94.79"
     ARG             8575..9182
                     /name="vanX gene in vanA cluster"
                     /gene="vanX gene in vanA cluster"
                     /gene_name="vanX; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.5"
                     /coverage="100.0"
     Typing          2..1041
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgatt ttaattatta caaatcaaaa gaaatttatc gtgaaaaata ttatcaaatg
       61 cctaaggtat tttttactaa cgaaaaatac atggatctat caaatgatgc aaaaattgct
      121 tatatgctac ttaaagatcg atttgattat tcagtcaaaa ataactgggt agattctgat
      181 gataatatat ttttcatatt cacagttgaa gaattaatga aattgttaca atgccgcgaa
      241 ggtaaagtat ctaaaatcaa aaaggaatta gaaaaagcag gtcttctgaa acagaagaga
      301 ggaagagtaa ataaacgtga cggtaagatt gaatccatgc ctaatttatt gtacttaggc
      361 aagccagaag taacgaatca agacgtcttt aaaatcatgg aagaggaaga caatactgat
      421 accaccgtaa ttgcgaaaat cgcaaatacg gcaaaaccgc gagaaatcaa tgataccacc
      481 gtaattgcga aaatcgcaaa tacggcaaaa tccagtcata tcaaggactc aaccgtaatt
      541 gcgaaaatcg cagacaatct attctattct aattctatag acactaatag acacataata
      601 gacactgaaa ctgatcagtt acaaaatcaa gttttactag ataacttcgt cgacatcatg
      661 aaagaggaca gtataaatac atttgtacca gaaaatgtat taaacttgat aaaaacattt
      721 tctagtactt acgaagacgc tcaaaaaacc gtccaaacta ttcataatgc aaagaaaaaa
      781 gctgaagaat tagagcaaac agttgttgtt tatgaagaac tggaatctta tggaattgat
      841 gctgacaaag gattatatat gactttgctg aaagcttatc aaaaacaaaa aactgaaaaa
      901 gtaaacaatt tgcagaatct aattttcgtt tatgtaaaaa attggtttgt cgaaaaagct
      961 attgcggcaa agcttgcaaa cgaacaaaga gaaacattta acgaattacc aacagttagt
     1021 acggaaaatt ggctagagta gttgataaaa agcgatcact ataaaaatga tcaattttaa
     1081 attagaactt ttttatgatt ttaattttat tacaaatgtt acaaataaat agctattatt
     1141 ccggagtttt tagattaaaa gtttatgaaa acaatcacaa acgtaggttt taaagtcata
     1201 atcgttagat attttagtta tttttcattc aaaaaaatag ggaactagtg tttgcatatg
     1261 tgcttttttg agttataata attcatgaag agaggtaatt cacttatttc cccaagctga
     1321 aaagcctgat attacttctt ttcaggcagt gctgctaata ttcaattaca cttttttgta
     1381 tatttttagt tgttttaaat ttaattgtct gatatgcttg tgatgaattg attttcgttc
     1441 tgcacttttg gacgataaat tgtgataatt gaaaatacaa aaaaaagaaa ggcccctgta
     1501 aggctcggga gactggtaat ctccgtcagt gtcctacagg aaagcattaa gatgacgcct
     1561 taatgcgtgc ccttcagtgt tacggtatta cccgcaacgg acggttctgt tgcaaagttt
     1621 taaataaaga ataaaatccc ttacggtatc tatgatttaa gctgggattc ccaataatac
     1681 cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg ttttcttata
     1741 taatcctcga atggcttcca tgcctttaat cgtggtagag gcagtgcgta aacttcgata
     1801 gaatttattg cgtctcttta ctggacgatg gtcttgttca atcaaattat tcaggtattt
     1861 aatggtacga tgttctgtcc cttgataaaa gccgtattct tttagtttct taaaggcact
     1921 tgtaatagag ggggctttat ctgtgactac aaccttcggt tcatcaaact gcttcactaa
     1981 ccgcttaaga aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa
     2041 ggttaaacca tctgcatcga tggctcgata caaataatgc cattttcctt taattttgat
     2101 gtacgtttca tccattttcc atgaataaaa ggatttttta tttttctttt tccaaatttg
     2161 atagagtagt ttgccatatt cttgcaccca acgataaatc gtcgtatgag aaacgttaat
     2221 gccacgatca tataagattt cttgaacttc acgatagcta aggttataac gaagatagta
     2281 gcccacggct acaataatca catcctgctg aaattgcttt cctttaaaat gattcatcgt
     2341 cattcctcct gctatctttt tctattattc taccttattt gatagtagat ttaaaacttt
     2401 gcaacagaac catttatgtt acagacttaa ctcgaatcac tcgtagtaca caagatctat
     2461 ttgaattaat cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat
     2521 ggcttgattt atcagaagat aatccataca gccaattctt aattactgta atggctggtg
     2581 ttaaccaatt agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta
     2641 agaaagaagg aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga
     2701 attatgcggt aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta
     2761 ctaatgtatc tagggcttca ttatacagga aattatcaga agtgaataat tagccattct
     2821 gtattccgct aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc
     2881 ctcctagcga tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt
     2941 agtcatttat tatgtaaatg cttataaatt cggccctata atctgataaa ttattaaggg
     3001 caaacttatg tgaaagggtg ataactatga gcgataaaat acttattgtg gatgatgaac
     3061 atgaaattgc cgatttggtt gaattatact taaaaaacga gaattatacg gttttcaaat
     3121 actataccgc caaagaagca ttggaatgta tagacaagtc tgagattgac cttgccatat
     3181 tggacatcat gcttcccggc acaagcggcc ttactatctg tcaaaaaata agggacaagc
     3241 acacctatcc gattatcatg ctgaccggga aagatacaga ggtagataaa attacagggt
     3301 taacaatcgg cgcggatgat tatataacga agccctttcg cccactggag ttaattgctc
     3361 gggtaaaggc ccagttgcgc cgatacaaaa aattcagtgg agtaaaggag cagaacgaaa
     3421 atgttatcgt ccactccggc cttgtcatta atgttaacac ccatgagtgt tatctgaacg
     3481 agaagcagtt atcccttact cccaccgagt tttcaatact gcgaatcctc tgtgaaaaca
     3541 aggggaatgt ggttagctcc gagctgctat ttcatgagat atggggcgac gaatatttca
     3601 gcaagagcaa caacaccatc accgtgcata tccggcattt gcgcgaaaaa atgaacgaca
     3661 ccattgataa tccgaaatat ataaaaacgg tatggggggt tggttataaa attgaaaaat
     3721 aaaaaaaacg actattccaa actagaacga aaactttaca tgtatatcgt tgcaattgtt
     3781 gtggtagcaa ttgtattcgt gttgtatatt cgttcaatga tccgagggaa acttggggat
     3841 tggatcttaa gtattttgga aaacaaatat gacttaaatc acctggacgc gatgaaatta
     3901 tatcaatatt ccatacggaa caatatagat atctttattt atgtggcgat tgtcattagt
     3961 attcttattc tatgtcgcgt catgctttca aaattcgcaa aatactttga cgagataaat
     4021 accggcattg atgtacttat tcagaacgaa gataaacaaa ttgagctttc tgcggaaatg
     4081 gatgttatgg aacaaaagct caacacatta aaacggactc tggaaaagcg agagcaggat
     4141 gcaaagctgg ccgaacaaag aaaaaatgac gttgttatgt acttggcgca cgatattaaa
     4201 acgcccctta catccattat cggttatttg agcctgcttg acgaggctcc agacatgccg
     4261 gtagatcaaa aggcaaagta tgtgcatatc acgttggaca aagcgtatcg actcgaacag
     4321 ctaatcgacg agttttttga gattacacgg tataacctac aaacgataac gctaacaaaa
     4381 acgcacatag acctatacta tatgctggtg cagatgaccg atgaatttta tcctcagctt
     4441 tccgcacatg gaaaacaggc ggttattcac gcccccgagg atctgaccgt gtccggcgac
     4501 cctgataaac tcgcgagagt ctttaacaac attttgaaaa acgccgctgc atacagtgag
     4561 gataacagca tcattgacat taccgcgggc ctctccgggg atgtggtgtc aatcgaattc
     4621 aagaacactg gaagcatccc aaaagataag ctagctgcca tatttgaaaa gttctatagg
     4681 ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac ttggattggc gattgcaaaa
     4741 gaaattattg ttcagcatgg agggcagatt tacgcggaaa gcaatgataa ctatacgacg
     4801 tttagggtag agcttccagc gatgccagac ttggttgata aaaggaggtc ctaagagatg
     4861 tatataattt tggctctttg tcaaatgatg ttggtaaaga gaaatcttag aataattgga
     4921 gatcaagtgg atcagttcct cttgatcttt tttagttgaa aatcaatgcg ttgccatgac
     4981 gaatcatgat gcgcaatttg aaattataaa agctacgaaa gccatatgca tttcgtttta
     5041 acactttgat atggttgttc agacattcaa gtgggccatt tgaataactt tgttgtaaag
     5101 catttttgat ttgtggcaga aatttctgga acgttctcaa ggtcgtctga tagccttcgg
     5161 gaagcatgat attattggta agtagttctt caaacgatac gtagtctcgt tcctttacag
     5221 cgtaaagaaa gtcttgatat aactgatagc ctacacgcaa agcagggctc cctttcagta
     5281 agcgatccac aatatcggtc tctgttaaat gagctttgaa gccaggtcgc caaaggcgtc
     5341 gagtataatc aagcttgcgt tcatcttttt gaagaagttt ccagtatctt tttaattgct
     5401 tacctaattg atagtgtttt tgttctttgc tttttaatag ttggttcgtt tctttgattc
     5461 gatgattgcg aaaggtacga ccaatatgtt ggacgatgtg aaaccggtca ataatcagct
     5521 gtgcttttgg aaacagcttt ttaacgagcg atacataagg agcgtacata tcaatcacga
     5581 tccattcgac cgcttcacga atttctcgtg aaaaatgact aaaataacgt tttaaaaaag
     5641 gcagttgtcg gttctcgaca atgtccaata agcgctgtgt ttgtccatcc atcatgatga
     5701 aactcatagc acctgaaaca ccccgaactg atttgaattc atcaaaacaa agtaccttag
     5761 gtaacggtgt ccgaaaagga ttcaacggac gataaaatcg cttcataata cggtaaacag
     5821 aagagaccga tagatttttc tgagcggcga tctctgtcat tgaggtattt tttttcagac
     5881 gagtcacgac agaccagcgg actttctcac tgatgaaaca atgttttttc gcaacagaag
     5941 tatctgcgac aaaggtccgc tgacaagctt tgcatttgaa gcgttgcttt ttcaattcga
     6001 ggtaggtacg gtattcagaa acatcgttca ttaacaaccg gacggttgtt gtaccccaac
     6061 gcacaatatc tgaagcaatc tggtgtttac agtggatgca ttcttgggct gtataaatca
     6121 agtttccacg ataaatatga cagatttgcc ctttgtattt ttcttttgtg agacaatttt
     6181 cgttaaaagt aactgataag tctagaatat ctaatgtttc tttgataagg tgagtgtaag
     6241 acatcttgat tcctcctttt gggttggttc gccaaattaa ttctaaagga tttcaaggtg
     6301 tcttttctat atgttttgga aaataaaaaa gatgctggtg tcatccacca acatcaaaaa
     6361 gtatagagcc ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat
     6421 taacaattta atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac
     6481 gagccgtttt cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta
     6541 tttggtgcct ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt
     6601 atggatgtga gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta
     6661 tggcaacgat aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc
     6721 aatgtatcag tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga
     6781 gagccggtgt gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg
     6841 ctgctaagag aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg
     6901 attatactat gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg
     6961 tggaaaaaca tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag
     7021 ttggtgtggt gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg
     7081 gatgtaaagt gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg
     7141 atgagttgct gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc
     7201 actatattat cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata
     7261 ctgggcgcgg tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac
     7321 tgggcggtgc cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca
     7381 cccaaaaacc aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa
     7441 tcacaccgca tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca
     7501 ttaaaaactg tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata
     7561 ctgtttgggg gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc
     7621 gctaacatta ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta
     7681 tggaaaatgt gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta
     7741 ctctcgccgg ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc
     7801 aaccatgttg atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata
     7861 caaggtctgt ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca
     7921 atttgtatgg acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc
     7981 gccttttggg ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt
     8041 tttgttaagc cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac
     8101 gaattggact acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag
     8161 gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagctgtt
     8221 ggcgaggtgg accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag
     8281 ccggaaaaag gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag
     8341 cgaggacgga tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta
     8401 gcccgtgtgg atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact
     8461 ctgcccggtt tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca
     8521 cttcccgaac tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa
     8581 taggatttac ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca
     8641 cttgggataa tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga
     8701 catacgagtt ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg
     8761 gattgcttct atgggacggt taccgtccta atcgtgctgt aaactgtttt atgcaatggg
     8821 ctgcacagcc ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg
     8881 agatgatttc aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg
     8941 atcttacgct ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt
     9001 ttatggatga acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc
     9061 gcagacgttt gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt
     9121 ggcactatgt attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat
     9181 aaacttttaa ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga
     9241 cgtatgtaac tggttcttag ggaatttata tatagtagat agtattgaag atgtaaggca
     9301 gagcgatatt gcggtcatta tctgcgtgcg ctgcggcaag atagcctgat aataagactg
     9361 atcgcataga ggggtggtat ttcacaccgc ccattgtcaa caggcagttc agcctcgtta
     9421 aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata gtaaatccag
     9481 tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca aacgagattg
     9541 tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata gttaaggagg
     9601 agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat atacttaggt
     9661 tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca aaattatgat
     9721 caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca agagaaaaca
     9781 attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa atatcctgtt
     9841 cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga attaataaat
     9901 ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca aaaattttca
     9961 gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa tagtggctat
    10021 cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta tgccttacca
    10081 gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag cttgacgaaa
    10141 atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata cgggttcatt
    10201 ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaact atggcatatt
    10261 cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt tctcgaggaa
    10321 tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg ggaaaaatat
    10381 gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac taatcttcgt
    10441 tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc cggatcaaca
    10501 catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa tgaacagcat
    10561 tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca cgttaactag
    10621 gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt atattgggaa
    10681 aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg ttagtgttat
    10741 tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt cttaacttga
    10801 ctccatttac tgctactggg aatttcagag agatgataga taatgttata atctttattc
    10861 catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct aagtttgctt
    10921 ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct attggagcga
    10981 cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg aaattatatg
    11041 gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt tttgtaggta
    11101 tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat tacgtgtaag
    11161 atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg aattggatta
    11221 gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg aaaaccggtg
    11281 ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta tcaattttag
    11341 tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc attttcctga
    11401 cggtaccccc aaactaagat ttcatagata gattttctga taactttaaa aaacacgaat
    11461 agcggaaagg atgtcttcat tcgctaaaga gtaataaact acttttccat cttgacgtga
    11521 ttttgccatt ccatgttttt ttaaaaaacg taaatggtgt gacgttgtag cgacactagc
    11581 agtaagtatt tctgctaaat cacagacgca catttcctta taggtttcta aagcatagaa
    11641 aattttaatt ctagaaaaat cgctgaaaca tttccctaac acaagtaagt ttgaaaaatc
    11701 tttttgatcc aatttatttt ttattccatt tactttttct ttatgtacga cagtcacttg
    11761 gcaaactttt ttcattcatc tcacctcaca ttcaaacgat tatttgaatg aagtatacct
    11821 atttgatcac tcttttgtca atctacctta attccaatac actattataa gcaatttcat
    11881 gtattgaagt aaacttactt gcaagtgcgt ttttgattcc aagttaaaaa catattatta
    11941 ttctacataa tttcctaatc aaataaaact cctttgaaaa ttgttctaaa ggagttttat
    12001 tattttgtat ttctactaaa aatagcgtac aatgtaatta ctaagtaatt acgaaatgta
    12061 aaataatgga ggaaataata tgattacaaa aacaagaaaa caaggaaatt ctattatgtt
    12121 aacggtccct aaagaattcg atgtgcccaa tggtgttgaa gtagaagcta agcttgtaga
    12181 gaatggtatc ctttatgagt ttgtagagcc taaaaaagaa ttttttgatt ttagtgaaga
    12241 tattttggct gatattcttt cagaaggtta tagcaaacaa gagatcttaa aagaatttag
    12301 aaatagaaaa aatgcgttaa cttctgcatt taaatctatt gctgaagata ctgttgcaaa
    12361 ttctaaaccg atgacaaaag aggagttggc agcagaaatt ggcttatgag attctacctt
    12421 ctaaacacgt aataaagtat ctaaaaaaat taaaagagaa aacattaaaa gaacaatttt
    12481 taacgataat ttatgatgag atagcagttc gtcctcattc gggagaacaa aaaacgggtg
    12541 atttatcagg aatttgggct attggattca agtatgcagg tacgacatat cgcgttgcat
    12601 atgaaattaa agataatgca gtgataccta acttactatg tggaacgcat gaaaattttt
    12661 atgaacaatt aaaaaagatt aggtaagaac ccaatgtttc aacttgagag gatagacgac
    12721 actttaaaag ataattttta acagctgaga gttgtaatac cctataattt taaaccacta
    12781 acctatatat ttatgaaaaa tagaaaaaat taaaacgcat tttaagcctc tttcaaatta
    12841 aaagcttcat ccttatgcac aaagagggct taaaatgcgt tattttatat tgttttttag
    12901 taataataaa aaaatcactc taattctccc ataaatatta tagattccta aagattaaaa
    12961 tagattattc cctattgtct tttaagtagc ggtagagtgt tgccttgcta ataccagtca
    13021 tttccgtaat ttcttttacg ctataaacct gacttttata caattttact gctcgatcaa
    13081 catctttctg gttcactcgt ggacggccac ctttacgacc tctcgcacgt gcacttttca
    13141 aaccttcttt ggtacgttca acaattaaat cacgttcgaa ctgactaaat gcttggaaga
    13201 cagtcatcat taagcgtccg tgaggcgtgc tggtatcgaa gttttctttc aagctcacca
    13261 gcttaacatg gtgttcttca aaataggtaa caaggtcaat caaatccttt gtactacgac
    13321 ccaatcgaga aaaactttct acaattaccg tatctcccgg acgaatttta tcttttaaac
    13381 gatttagttg gggacgaacc gctttggttc ccgtcatttt ctcagttagg atttcattac
    13441 aattttgttc attgagtaag tctaattgac gatttagttc ttgtgaacgt gtactcactc
    13501 gggcataacc ataaatgtat ccactcatgc ttttcttcct ttcgtacttt aattctggct
    13561 tgattgtatc ataaaaggga gttatagata catagttaat gcaacaagtt atgatacaaa
    13621 atatgacctc ttttatttaa taaaagaggt catttcattt tgtctcataa tcgattgttt
    13681 ttgatacgag tgtgtatacg aaataaacaa aaaaatctta agtaaatttc tagataccta
    13741 atttaatcct aactagtgta tcctaaagtt aataacgagt ttatcacaat gaaaattata
    13801 aaatgctccc atattagata taacatgcaa tatttgaatt agaactacaa aatttattcg
    13861 ttttataaag taaataatgt tgagatacta aggaatagaa tattataagg aattgagctg
    13921 aatgtattgg ataaaaaaag aataacttta ttagagatgg actataaaaa tgctcgtaat
    13981 tttcttctga agtctgaaag ctatttgaat gctcagctac cagaatatat tagttttgac
    14041 gaaactttaa aatctgctaa aaaagtttta acttcgccgt ttgtataatt aagcgagaca
    14101 aataaaaaag ccatttatgt tacactcaaa ttgtaatacc cgctaaagca tacaaggaga
    14161 gtgaacataa atgacttata cccatcttac accaaacgag cttgtaatga tagaagcata
    14221 ttttcatcaa gaaactccgg ttgctatcgt tgcgaagcag cttaaacgtg gacgccaaac
    14281 aatttacaat gtctataact ttctcaaatg tggtggaaca gcacttgaat actttgaaca
    14341 atacaaagaa aataagcgac gttgtgggag aaccgaaatc atttttcctg ctgaggaaaa
    14401 agaatacatt gaaaaaagat caactgaagg ttggacccca gacgtcatta ttggccgtgc
    14461 agagcgaacc ttctcttgtt cagtaagtac cctctatcgc cggtttaaga cgggagaatt
    14521 caatgtttta catttaccga tgcaaggaaa acgaaaacca aatggttata aggaaaaacg
    14581 tggaaaacag gcattcaaaa gaaatatttc tgaacgtaaa aaagattatg tcgttttcga
    14641 agaagaattt ggacatttag agggtgatac gattgtcggc atccaccata aaagtgccgt
    14701 catcacactc gttgagcgac tttcaaaagc cattatcgcc ttgaaaccag aaggccgtaa
    14761 ggcagttgat attgaaaatt cgattaatga atggcttcaa tccgtaccca aaaatctttt
    14821 caaatcaatt acctttgatt gtggaaaaga attctctaat tggaaaagta ttagtaatac
    14881 gaatgatatt gatatttatt tcgcagaccc aggaacgcct tcccaacggg gattaaatga
    14941 acattcaaac ggactcctcc gaaaagatgg actaccaaaa gaaatggaat tcaaccaagt
    15001 caatcaagga ttcatctcat ccgttgcgtc caaaagaaac catatcccta gaaaatcact
    15061 aaattaccaa acaccattag aagttttttt gagttacgta aatggaaagt tttgtctcgc
    15121 ttaatttgac aaataatact tcaaaaaaaa accaaccgaa agatataaaa gatattgtag
    15181 atacacgaat gtataaagaa cgtgatgact taaactatac gcttattatc aataaagatg
    15241 gaaactattc atggcgtcct ctcgttctac tacatcctat tctatatgta gatttggtta
    15301 attgtctcac taaagaagac cattgggagg agttaaaaaa gaggtttagt gaatttaaaa
    15361 aagataaaaa tattaactgt tattctatac ctgttgaagc ttccaaaggt tcaaaaagga
    15421 cagatcttgg tgaaacaata ctaaactggt gggaatattt tgaacaaaaa agcttagctc
    15481 agaatattaa ataccaatat tgcatgttta ctgacatcgc tgattgttat ccatcaattt
    15541 atactcactc tataacatgg gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata
    15601 agtgttcggg ctcacttggc gataaaatag ataaaaaaat atctggaatg caacacaatc
    15661 aaacaaatgg tatccctcag ggttccgttc ttatggattt tgtagcagag atcgttctag
    15721 ggtatgttga tttgagatta tcgaaagaat taaaaaaatt agaaaattta aaagattaca
    15781 aagtactcag atatagagat gattatagaa tattttcaaa taatcgtaat gatttggaac
    15841 aaattatgaa agtgttatca gaagtcttat atgatttaaa ttttaagtta aattctagta
    15901 aaacaaaatt atcagatgat attatcatag attctatcaa aaaagataag ctctattggg
    15961 aagaaattaa ggcaagtata agaatgaatg ccacaaactt agatggtgat agagaaattc
    16021 gttatagtat aaatttacaa aaacatttat tggaaataaa aaagttagca gataagcatc
    16081 ctaattcagg ccaattgaaa aaagcacttt ctgagtttaa taaagaacgc attattaaaa
    16141 tcacttcaaa gccgaacgat cttttaccac ttattggtat cctggtagat atcatgataa
    16201 ataacccggg ttctttgccg cagtgtgtct taattatagg gcagttatta aatttcgaaa
    16261 atgagagtaa ggctagagaa atagttgaaa tgattataaa caaatatgct ttaaagccaa
    16321 atactgaatt tatgatgata tggtttcaaa gattgaccac catttttgga gaaatgtttg
    16381 atagtgtttt atcatccgaa tttgtatcta aaatcaaaga tcctgtctct aatcagatgt
    16441 tttctgtaga ctggctaaat gaagatgcta agaaatcttt ttatgaaggg accattaaag
    16501 acgaagagaa atatattaaa atgagtaaga aaatatatat tactgaattt gatgatttta
    16561 atgattatcc tttttaagtg aaaatttaga atgaatataa aaaacattac aatctaattt
    16621 ttttaaaaaa atgttatatc ggaagtaatc tcttgatttc tatactataa gataagaaaa
    16681 ccactattta acaaggttat tcgaacctta ttttaagtta cttattattt aatgtatcga
    16741 aaaaggtgaa ataggtttgt tataataaga attaagtaag acatgaattg tctaagcaaa
    16801 tgtagcttat ccatttcatt aaaatacata tggtgaaaaa acaagttcaa atagtaacta
    16861 attttttaaa aaactgattt ataaaaaata caacacgaat gaggaaaata accaatgaca
    16921 gaacaaatcg aaaaaaattc aaaaagctta tatcaggcac tgtggaattc cgcagatatc
    16981 ctgcgttcaa aaatggacgc taatgaatac aagagctatt tactaggctt ggtcttttat
    17041 aaatacttat ccgacaacat gctgcggtat gtctccgttc tattggagga agaaacggac
    17101 gacctaaata aagcacaaga cctttacgtt gaagcattta aagatgcaga tataaaggat
    17161 gatctcttgt atgaactaaa agatgagttt tcatatacca tcgcacccgc attgaccttt
    17221 accgcgcaag tggcagccat tcatgatgga tcttttcaat tagaagacct ggtacaaggt
    17281 ttccgtgaca ttgaacaatc cagcgaaaac tttgagaact tatttgaaga cattgatctg
    17341 tattctaaaa aactggggtc aaccccacaa aaacaaaata aaaccatcgc agatgttatg
    17401 aaagaattag aagggttaaa catggcgggg catgctggcg atgtcttagg cgatgcctat
    17461 gagtacttaa ttggccaatt cgcatctgaa tccggtaaaa aagccggtga attctatacc
    17521 ccacaaccgg tcgcaaaatt gatgacacaa attgttttac aaggaaaaga agataaaaaa
    17581 ggcttttctg tttacgacgc gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa
    17641 tattcccatc aaccgggaac catttcttat ttcggacaag agttgaatac gtctacctat
    17701 aatttggcgc gtatgaacat gattttgcat ggggttccca ttgccaacca acacttgcat
    17761 aatgcggata ccttggatca agactggcca acagaagagc caacgaactt cgatggggtg
    17821 ctcatgaacc caccgtattc tgcaaaatgg tcggctgata aaggcttctt agatgaccca
    17881 cgcttctctg cctatggcgt attagcccct aaatcaaaag cggactttgc tttcttatta
    17941 catggctact atcatttgaa agatacgggc gtcatggcca ttgttttgcc acatggggtg
    18001 ctctttagag ggggcgcaga agggaagata cgcaaaacct tgttagaaaa tggtgccatt
    18061 gacaccgtca ttggcttacc tgctaacatc ttctttaata cgtccattcc aacgaccgta
    18121 attatcttga agaaagaccg tgcgaaaaaa gatgtgctct ttattgatgc gtcacaagat
    18181 tttgaaaaaa taaaaacgca aaatacgcta cgagattacc atattgacgc tattttagag
    18241 gcctataaaa cccgtacaat taaagacaag tatgcttatg tcgcagaatt tgatgaaatt
    18301 gtggagaatg attacaactt aaacattccc cgttacgtgg atacctttga agaagaagaa
    18361 gggttctgtt gcaaagtttt aaataaagaa taaaatccct tacggtatct atgatttaag
    18421 ctgggattcc caataatacc ttgatttcag tacagaccga aaacccgaag agagtgcctt
    18481 cttttcgggt tttcttatat aatcctcgaa tggcttccat gcctttaatc gtggtagagg
    18541 cagtgcgtaa acttcgatag aatttattgc gtctctttac tggacgatgg tcttgttcaa
    18601 tcaaattatt caggtattta atggtacgat gttctgtccc ttgataaaag ccgtattctt
    18661 ttagtttctt aaaggcactt gtaatagagg gggctttatc tgtgactaca accttcggtt
    18721 catcaaactg cttcactaac cgcttaagaa aagcataggc tgcttgtgtg tcccgttttt
    18781 tacgtaacca aatatccaag gttaaaccat ctgcatcgat ggctcgatac aaataatgcc
    18841 attttccttt aattttgatg tacgtttcat ccattttcca tgaataaaag gattttttat
    18901 ttttcttttt ccaaatttga tagagtagtt tgccatattc ttgcacccaa cgataaatcg
    18961 tcgtatgaga aacgttaatg ccacgatcat ataagatttc ttgaacttca cgatagctaa
    19021 ggttataacg aagatagtag cccacggcta caataatcac atcctgctga aattgctttc
    19081 ctttaaaatg attcatcgtc attcctcctg ctatcttttt ctattattct accttatttg
    19141 atagtagatt taaaactttg caacagaacc cgaagaacaa ttgtatttgg aaagtgaaac
    19201 gagtcaaacg aaggtcacag tcagccataa tcgtttagac gtgtataaac gtcgtcctga
    19261 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    19321 ttacctagaa aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt gcaggaaaca
    19381 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    19441 aatggaagag ctaaacagca aagatgcgac gatagacgat tattaccttg tatttactag
    19501 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    19561 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    19621 cacgacttta actgattttt tagacaccta tacttacgac gatacagaag aaatccaaaa
    19681 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    19741 atttataccg aaaagtgaga atgtgtgaaa taaggacaaa aaacctagta actaagggaa
    19801 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    19861 gcgggcaaca cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    19921 ctattgttag tcctatttgt tggcgaaata cttatgcgcc atgctgcagc tgtgcattta
    19981 aacttcgtag tatttttggc aatactggta ttttgtttga tcttttggcg ctttgtatta
    20041 gcccttgtga gtctactatt caagatgatt gcagccatat ttttcatagg gttatttcga
    20101 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgttacgttg
    20161 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggcaa aagtgaatcc
    20221 aggattatgt gtattgcgtt agatttattg attgcccaag aaaaagcagg atttgaggta
    20281 ccagcactca aaaagtgacg tcacttttta tcctaaaaac taaaagtgat agcactttta
    20341 attataagaa gttagaatat taatcatttg cttaattgta caatataatg tacaattgtt
    20401 ttatagaaat aaataagggg tgaaaggaat ggaagcagta gcttattcaa atttccgcca
    20461 aaatttacgt agttatatga aacaagttaa tgaggatgct gaaacactta ttgtaacaag
    20521 taaagatgta gaagatacag ttgttgtatt atcaaaaaga gattatgatt ctatgcaaga
    20581 aacgttgaga acactttcta ataattacgt catggaaaaa attcgtcgag gagatgaaca
    20641 attctccaaa ggtgcattta aaacacatga cttaatcgag gttgaatctg atgattaagg
    20701 cttggtctga tgatgcttgg gatgattatc tttattggca tgagcaagga aacaaaagca
    20761 atataaaaaa gattaacaag ttaataaaag atatcgatcg ttcccccttt gctggattag
    20821 gaaaacctga gccattaaag catgatttat ctggaaaatg gtccagaaga attacagatg
    20881 aacatagact gatatataga gttgaaaatg aaacgatatt tatttattct gcaaaagatc
    20941 actattaacc aatcggaagt aaggaaaggg tcagaaactt aaaagttttt gatccttatt
    21001 ttatttaccc tagtcattta aaaagctaat atagcttagt gttgattgtt attaatgaat
    21061 gtgtttgtta cgcgtattac ggatataagg ttagtaaaat catttctaaa gttgaggaaa
    21121 agtaaatata aatggcttaa atttcaacaa tttgaagttg aatagatatg ttataatact
    21181 attgtagtgt gggatgttag ttactaaagg atgacgctta tatatatgac tgaatagaat
    21241 aagcaatagg tttaataatc tattttaaat tttttgtact agttttagtt aattagcaaa
    21301 aacaacaaaa ataaacttct catagaattt agctaaaaat taatgattta tttacatatt
    21361 aaatttggat acagttaagt aatttttata tattggagga gaagtaatgg aatataaatt
    21421 taacttgaat ttgaaagaag tactccccac tcagggaaca ataaacataa atggtcaaga
    21481 taatgatgac gatatagtaa taaaaaaata gaatttattg attctgatat aaatgttttt
    21541 ttttattcta aagaaaaatt agctgatttt agcaatattg ctgaaatgat tgaacgttta
    21601 aaagatttta tgatcagtgt ttgggatgta gcgatgcttg ctgacgaatc aaattatact
    21661 tcgagcatat acagattaga agatagtaat tggatagaaa tttcattaga gttaaagaca
    21721 cttcctaatt ccgttaacta tataattaga gatataactt ctgaagctca acaattagtt
    21781 aattacatgc atggacacga aagtatcccc ttttctctta tactccttaa aagaagtaaa
    21841 aatatatcaa atactagaga taggtttatt tcgataatta cagcctgtga aataggggta
    21901 aaggaatttt atcaacaaga gaaacctgat ttaactctaa tatttgagaa tttacaatct
    21961 ccccctgtag acaaattatt agggagcatg tttaaagggt actttaatat tgagtttccg
    22021 aaagaaataa gaaaggaaat taaaaatttc atagaaaaac gtaataaatt ggttcattct
    22081 cctcaaggaa gtaaaccaac tatagaagaa tgttttgaat gttatatggc agtgctaaaa
    22141 acatttaatt ttttttataa aattaatgat agcctgaata attcatactt ttaattaaaa
    22201 ccatgtgaaa ctgcctcacg gcagcttaca attacttttt catcttcacc cgttaagtga
    22261 tgaaaaaaga acccctttct gctatggtta aagtgactaa accaaccaaa agaaaggagt
    22321 tcttttcatg actagcttac acaaaaacca agtaaaattc aattcaaata tcactatttc
    22381 tcatacaggt ggtcaattat cgagtgattc gggtctcgtc ctagtgaaag agctgatgaa
    22441 catctttggt ttttctgaac tggctaagca acacattcac attgaagacg aacgagcgta
    22501 ttttactcat gacaacttat ccatacttga gcagttcatt atgcaattaa ttgctggtta
    22561 ctcagctgat tctgcagcta atctcctgag acaagatcct gtgtttaaag ctgttttaga
    22621 taggaaagaa ctcgcttctc aatcttcact ttcccgattt ttagatcgac tatctgagga
    22681 gaatatccat gaacttcaag ctttgaacca agaacttatc gataaagcac gcctcatccg
    22741 taatgatacg gaattaatca ttgatttaga ttcgacccat tcagatacat ttggtcacca
    22801 agaacaaacg aattataata cacactacca aacctatggt taccatccat tggtagcttt
    22861 tgacggattg actggtgact tcttaaaagc tgaactacgt tcaggaaatc aatatacatc
    22921 aaaaggcgta aaggagtttc tcacaccttt attagagcac tataatcact ctctaccaaa
    22981 cactgacatc ttggttcgtg gagacagcgg gttcgctaca cctggtgtgt atgattcgtg
    23041 tgaatcaaaa aagagtcatt atgttattcg actgaagaat aatcgtagac taggtcaaat
    23101 agctgagaaa tcagtacttt atggcgataa tcaaaagtgg gaagaacgag aagttcagta
    23161 cttctcgacc acttatcaag cacaatcctg gtcacaaagt cgtcgcgtgt gtatacgctc
    23221 aacacgtgaa gcgggcgaac tactctttcg acatgaattt atcgtgacga atctatcaga
    23281 aaatgtttct cctgatacca tcttttctat ctatgccaaa cgtggcacaa tggagaactt
    23341 cattaaagaa gcgaaagctg gcttttactt tgacaaaaca gacagtcctc gctttttgga
    23401 gaatcatgtc cgaatgatgc tcagcttgat agcttacaac ttagtcaact tcttaagaac
    23461 gattggcttt gaagaagtcc aaaagggaat gaccattcat tctattcgat tgaagtttct
    23521 gaaagttgct gggaaattag tccaaacggg tagacgagtc tatctcaaat tatctagtta
    23581 tcatgtgtat cagaatgaat tctacagggt ctttgctcgc ctgaggcgag ccagtcaatg
    23641 gatctaatcc aacaatctaa cgattttttt actgggaagt tatccaggga gaagtatgct
    23701 caaaatgcct tacacccaaa atttattcct caatattttt gaacagtgaa tgtttgaagt
    23761 aactttttag tcaaaatcag ttgataggga tattagtgat acatatttca ataaaatgtg
    23821 ccaaagaatt aaagctatga attattcagg gatagcttta tgtatagaga tttttatgaa
    23881 gaatctatag aattgactcc aacggaacca aataaatctc aaatcacttt gtcaaaggag
    23941 ctagagagaa aagtagagtc tcaagaacta gctataggaa ttagtataac caatgtgtcg
    24001 acatacaaag aattatacga agctaaatca caagataata aggattttgt agaaaatata
    24061 gataaggaac caacaaatac agtaggggat taacgtaaat aagtgaagat tatattaaca
    24121 gactctttat ttagttaagt aaaaaatggt ttgttggaat tttatgcttt atctgttctg
    24181 agtaagactt aattttatag atagtaatta tatgtaggaa attaaagatg agaagataat
    24241 aaaagcagca tgatcaacta tttgtcattg accatgctgt ctttttttat tctatacacg
    24301 gtttgtctcg taacattgta gtctttagca atcttagcaa tcgcaacacc atttttaaga
    24361 tcttcaacga tgcttttgta aacaaggcga cgttggggat cttttgcgtc tgcactgtat
    24421 aatttgggcc gccctttgta gacgccgttt gcttttgcga tcttaattcc ttgtgcctgg
    24481 cgacgtttcg attcagtccg ttcttgttca gcgatcatgg ccaaaatttg gatgatcaga
    24541 tccttaatga atttatccag gagaggattg ccaattgctt cagccatgat cggtaaacta
    24601 gtaatgatca gcttcacatt tttcttcttc aagtaattga ctgaagcgat gatctcgtca
    24661 taatttcttc caagtcgatc aatagcttcc acaataaaaa tatcttgatc tcggacgtaa
    24721 tctaatgctt cttggaaaac aggtcgatga gtaatcgttg cgccagattg tttctcgatg
    24781 aaaattctct ctgcaccgaa ttttttcatt tcttcaatct ggcgttgttc attttgatcg
    24841 atcgatgaga cacgtatgta tgcaactctc aaaactttta cctactttct gcaactattt
    24901 aaaatacacc ctaatcatac acgaggaagc catatttttc aatgttatac cagtgtctat
    24961 ttagggtaaa ccttatatag acaaaaacta atgatggagg aaatagtatg agaatttttt
    25021 gtaagtgtct actgataact tattctcaaa tgttgtaagg attttattca tttgttaata
    25081 ttaaagaacg gaactccaaa aaagtccgta ctctaatttg cagtctctca ctcttttaga
    25141 gtgagagttt ttattagatt ttcaaattaa ttagtaaagt gatagaataa attgatcata
    25201 caaatcaaaa gaagaagcag ggtgatagca tacctatgct tcttttttta tgtatttagc
    25261 tatttcttct ttacaatatc gaacatatgt ttgtataatg tgaaagaatg gagtgttatt
    25321 tatgatggat tattcaaaag agccagtaaa tgattacttt ttaatcgata tgaaaagctt
    25381 ttacgcaagt gtcgaatgta tagaaagaaa tttagatcca ttaacaacag aacttgttgt
    25441 tatgagtcga gctgataata ctggttcagg attgatatta gcttcttctc ctgaagcaaa
    25501 aaagcggtat ggtattacaa atgtgagtag accacgtgac ttaccacaac catttcctaa
    25561 aacactacac gttgttccac cacgtatgaa cctatatatc aagcgaaata tgcaggtaaa
    25621 taatattttc agaagatatg tggctgatga agatctactg atttactcga tcgatgaatc
    25681 aatcttaaaa gtgacccgat cactgaatct ttttacgact gaaggaacac gaagtcaacg
    25741 gagaaagaag ctggctcaaa tgatccagga gaatattaaa gaagagctag gattgattgc
    25801 tacagtaggt gtcggagata atcccctttt ggctaaacta gccttagaca acgaagcaaa
    25861 gcataatgaa ggatttgttg cggaatggac gtacgaaaac gttcctgaaa aagtatggaa
    25921 tattcctgaa atgacggact tttggggaat tggctctcga atgaaaaaaa gactcaatca
    25981 gatggggatt atgagcatta gagatcttgc taactggaat ccttatacga ttaagaatag
    26041 actaggtgtg attggcttac agctgtactt ccatgctaat gggatcgatc gaacagatat
    26101 agcaatccca ccagaaccga caaaggaaaa atcatatgga aattcacaag tgttaccacg
    26161 agattatact agaagaaatg aaatcgagct ggtggtaaaa gaaatggcgg aacaagtagc
    26221 gatacgcatt aggcagcata actgtaaaac aggttgtgtc catttacaca ttggcacgtc
    26281 gatcttagaa acgagaccag ggttttcaca ccagatgaag atccctatca cagataatac
    26341 aaaggaattg cagaattatt gtcttttctt gtttgataag tattatgaag gacaagaagt
    26401 aaggcatgtt ggaataacct atagcaagct tgtgtacacc gattcgcttc agctggattt
    26461 atttagtgat ccacaaaagc aaattgacga agaaaattta gataaaatta ttgacaagat
    26521 tcggcaaaaa tatggtttta cgtcgattgt acatgcttct tcaatgttag aaggcgctag
    26581 aagcattact agaagcacac ttgttggtgg tcatgcgggc ggaaatgggg gaattaaaaa
    26641 tgattgatta caatgatcgt ggacgtttaa agtgggcagg gttttattta tctgatcata
    26701 ctgaagaaat tgatgccgac aatgaacaac gaaccagtca aaatttagca aaagaacaaa
    26761 tgacgactga agagattgcg gaagtattga acgctgcagt cataaaaaat agatcagtat
    26821 ctattcaaaa agaagaacga aacgctgaag gatactatcc agaagatatc attggcaaga
    26881 tcttaggtag tgatgagctg gggatctacg ttggaaaaga caaaattcac tacgacgaaa
    26941 tacgtcatat cgaattttac caggataata aatggagtcg attagaatga acattatcca
    27001 gcaatatgag ctaaagtata taacctttga tcagctatca gaagagattt ggggttatgg
    27061 ccaacgtttg ataaatgagg ttggtgttga gcgcttctct ttctatgtag aagctgcagc
    27121 aggttatcat aattttaggt tttatatctt cccgttatat atataaggta ttttacggat
    27181 tctatagagt tatctctatg aatccttttt tgtttttaca aataggagta gatactagaa
    27241 taatgcgtgt gttaaactat tattgcaaac gcaataataa aagaaagtag gcttacgcac
    27301 catgtctatc aatacatttg tttactcgca cccgatcaat gtttacatta tcaagaattt
    27361 aggaatcact gtagaacaat tttgtgagtt atatgcctac ccacaaggaa cggttgcaag
    27421 ctggataacc cgacaacgta gaataaaatc attacctgct tcattcgttt atgatttaag
    27481 cctggcctct agtttaaaca tgtctgatgt ttatgaaaag ctcctgtctt tagaaaaaga
    27541 atatgattcc ttcaagatta aacatgataa aaaaataaaa aaacatatat aatatcaatc
    27601 acaattgtat aatattacga tatcttaata tcatgatatc gtaatattgt ttttttctaa
    27661 gaaaatgcac tataagaagg gaattattag taacaatata gacagtttag taatgtttaa
    27721 attattctag ttgctgctcg caaaacatat ttcttatcat taataaaaaa ttttaaagta
    27781 ataaaaagtg attattttaa tttcgaataa catatgaata atctttatat ccgaaccgat
    27841 atatatcttt aaaacaaaat aatcacacaa tattacaata taatgcatat acgatattgc
    27901 aatatcacaa tattatgata taattatatt ataatataag atgaatgggg gatattttaa
    27961 tggcaagaat attgtcagta gccaatcaaa aaggtggagt ttctaaaact acgactagcg
    28021 ttaatttagc agcatgtttg aaatttgttt acggtaaaaa tgtactttta gtggacattg
    28081 atcctcaggg aaatgctaca gataatgtag gtttcgatat agatggaaca aaccaaccta
    28141 caatttatga agtattaaaa ggggaggctg acattacaga tgctattctg gactataaag
    28201 gaattgatgt tttgccagcc gacatagccc tttcttctgc agagagggaa tttactcaag
    28261 taggaagtga acacaggctt aaaagagtac ttcaaccaat caaggacaac tatgattata
    28321 ttataattga ttgtccacca agtttaggaa tgcttacagt caacgcattt actgtttctg
    28381 atgaaattat cattccagtt gaagctgcgt atttctcatt aaaaggacta ataaagttaa
    28441 gtgaaactat tgaaacggta aaagaatata cgaacgacaa gcttgaagta cgaggagttc
    28501 tatttactaa gtataataag cgttataaca tttctaaaga aatgacgtct gcagcaaccg
    28561 aaatttctag agtcattggt gcaaaaattt tcaaaactta tattcgtagg acaataaccg
    28621 ttgacgaagc gcaagctgct ggatcagatc taatcaattt taataaaccc tcaacagctg
    28681 aagatgacta taaagcattt acagaagaat atttaaaaga agttggtgat tttgatggca
    28741 gaagataatc gcagtcaagc tgcagtaagt agattactag gaaataaaaa taaaaagaat
    28801 tttaaatcaa aagaagtaga agaaatcgaa ttaaccaaga aaaatataag taaagtcata
    28861 aaagatcaag gaagtagtgt aagaacagca gatagcattc gtataaatcc aattctaatg
    28921 tcagcactca aatattggac gactattgct gaatcagaca aaagtaaacc agacgttata
    28981 gaagatgcgc tattaaagtc catacctgaa gaatacttga ttgaaggata taaactagca
    29041 aagaaacaaa ataaaattta atttacattc aatatcacga tatcataata tcgtgatatt
    29101 ttttttataa aaatcagtag tatcaaagct gataagtcgc ttatcttcat attgtaatat
    29161 tacgatatcg tgatataata ttattgtaat atcgtgatat agttactatg agcttaaacc
    29221 ttgaaataga gcggtttgct ttaatttatt ttttaaggta aactaaaaaa agataaaaaa
    29281 agactactgc tttaacaaag tttggcgact tatcagcagt agaaaaatat atagtggata
    29341 gatatatttg tttaattcat tgtaacatag ttttgaacaa aacgctatcc tacagaaaga
    29401 ggattggct
//
