LOCUS       NZ_CP066387             6303 bp    DNA     circular CON 20-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 16_1 plasmid p16_1_6.3kb,
            complete sequence.
ACCESSION   NZ_CP066387
VERSION     NZ_CP066387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038069
            Assembly: GCF_016406485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/20/2024 03:07:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,275
            CDSs (total)                      :: 3,183
            Genes (coding)                    :: 3,018
            CDSs (with protein)               :: 3,018
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 72 of 165
            Pseudo Genes (incomplete)         :: 85 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 24 of 165
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066387.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 16_1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p16_1_6.3kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(join(6001..6303,1..387))
                     /locus_tag="JCP87_RS16175"
                     /old_locus_tag="JCP87_16175"
     CDS             complement(join(6001..6303,1..387))
                     /locus_tag="JCP87_RS16175"
                     /old_locus_tag="JCP87_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(394..672)
                     /locus_tag="JCP87_RS16180"
                     /old_locus_tag="JCP87_16180"
     CDS             complement(394..672)
                     /locus_tag="JCP87_RS16180"
                     /old_locus_tag="JCP87_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(632..1546)
                     /locus_tag="JCP87_RS16185"
                     /old_locus_tag="JCP87_16185"
     CDS             complement(632..1546)
                     /locus_tag="JCP87_RS16185"
                     /old_locus_tag="JCP87_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1528..1908)
                     /locus_tag="JCP87_RS16190"
                     /old_locus_tag="JCP87_16190"
     CDS             complement(1528..1908)
                     /locus_tag="JCP87_RS16190"
                     /old_locus_tag="JCP87_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2230..2433)
                     /locus_tag="JCP87_RS16195"
                     /old_locus_tag="JCP87_16195"
     CDS             complement(2230..2433)
                     /locus_tag="JCP87_RS16195"
                     /old_locus_tag="JCP87_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(2445..2978)
                     /locus_tag="JCP87_RS16200"
                     /old_locus_tag="JCP87_16200"
     CDS             complement(2445..2978)
                     /locus_tag="JCP87_RS16200"
                     /old_locus_tag="JCP87_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(3428..3598)
                     /locus_tag="JCP87_RS16205"
                     /old_locus_tag="JCP87_16205"
     CDS             complement(3428..3598)
                     /locus_tag="JCP87_RS16205"
                     /old_locus_tag="JCP87_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3614..4147)
                     /locus_tag="JCP87_RS16210"
                     /old_locus_tag="JCP87_16210"
     CDS             complement(3614..4147)
                     /locus_tag="JCP87_RS16210"
                     /old_locus_tag="JCP87_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4168..5118)
                     /locus_tag="JCP87_RS16215"
                     /old_locus_tag="JCP87_16215"
     CDS             complement(4168..5118)
                     /locus_tag="JCP87_RS16215"
                     /old_locus_tag="JCP87_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            5794..5880
                     /locus_tag="JCP87_RS16220"
                     /old_locus_tag="JCP87_16220"
     CDS             5794..5880
                     /locus_tag="JCP87_RS16220"
                     /old_locus_tag="JCP87_16220"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          complement(4169..5117)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(636..1546)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaacgagtg agttttttca tgtttttatc tgttaaacgt ttctcttgat ccacaaaaat
       61 attgttcatg tcctcataac aagcggtaaa ttcctccatt ttttgtgcta atgcttgctc
      121 ttttttcttt ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatgct
      181 tcttgaaaca cgtttattac cctctaaaac gccgaataaa ccacttaaaa cgattgataa
      241 ggcaaatata accccgacca ataaaaatcg cttaaatcga attttagagc cttttaatga
      301 atatcttgtt tgataagcct tatagctaag ccaaataatt cctaaaatac tggcagaaat
      361 gagtagatag ggtaaataga atgccatgtc tgtttattct ccccatacca accaaaaggc
      421 taataatgtt agtactaagc aacataaggg ggcaagaata tccatcgatc gttgaattaa
      481 agcctttttt tgttcttggt ttcgctggtc taatagcgct tggtggagtt ttttgacttg
      541 tgaatccatc gtagtttgta tcctttcttg ggtataaggc gcaagaaagg cacgttgtct
      601 ctttatttcg ttttcgatgt cttttaacgt tttagagcga taattcttgt tggtggtctg
      661 ttcctcgttc aacgtgttgt tcatggtttc ttcgagtcgg tctaatttcc gattgttcag
      721 gcgtaaaagt tcgttgttct cttcgtactt cttctgcagt ttcgcgaatg ctagctcgag
      781 ctcttcgtag ctcgttggtt cgttgttgat tgtaggttcg ttctcgtctg tccagctcat
      841 gtaaaatcgt ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt
      901 tgtggaccaa ttcgtaagta aaattttttc cgcgattcaa gcaatgaatg ccgaatgttt
      961 taagtgaatc acaaaattgg gtatacgttc gagttttttt gtttttcatt gtttgatcaa
     1021 tcgcaaaccg aatttcgtct ttccaaaccg gttcattttt ttcttttaat ttaatttcag
     1081 acatggttcg tttttcggtt ttctttcctc gttccgtgat tgttaagtca tgttcaagac
     1141 aaatctcgtc atttaggtca taacatttgt ttaaaaatgc ttgcccatgg gcttgaaatt
     1201 ttttcccagt ttcaagatta acggagttaa tgacaatgtg attatgaata tggtctttgt
     1261 ctgtatgtgt gtaaatcgcg atttggtggt taggggctat ttttttcgca tactctgccc
     1321 ctagttgatt ggctttttca gcggtaattt ctcctggtcg aaaggactga atcaataagt
     1381 gtgcttgtac acgatcgtct tttccataaa gcgtgcgtat ttgtttcatt tcacttttgg
     1441 cataatcaat gtcacagttt attcctgttt tgacggttgc tcgtggttct gcgtaattga
     1501 ttgctcggct acatgaagtg gttcgactta ctttaactgt tgccatatct tttgtagctc
     1561 cttttgcatg atttttaggt tttcggataa acgttgagca tcatcaggag caacgtcgtg
     1621 ttggtgtgta ttgcaccact tggcaatttg attgatattc cctccagctt tggctaaatc
     1681 gcgcgcgatt gcctgggcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg
     1741 tgccttggct ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag
     1801 ctttgcaaat tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt
     1861 atatttttta gaatgatcgc tagccaaatt ttgatgttgt tcgctcataa gtcccaccct
     1921 ttcagtacgt cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaatga
     1981 gtaaagctca ctaaaagggt ttggcaagct ctatatttgc tagccaaata cctccgtatt
     2041 tggtgcaaat aaccgcttgt caaaggagcg caagctcccc cttgaatccc cctcaaaatc
     2101 cacgctccac aatacaaaaa aaggctagca tttgctagcc actttttgta aagtttcgct
     2161 tatttcaccg aaggtgaaat tgttgctttg taatattcaa acaaccctat tcttctaaaa
     2221 tgtatcgatt tatttttttt tagactttat tatgtctaag acaactaaga ttaacactaa
     2281 aaataaacca gatttgtata attgccctcc gcttagcata ttaacaatta tagcaacagc
     2341 tatagcaaat ataaaaatac gaatagtagc tatttgcgga ttctttttag taaatgtttt
     2401 taatgatttt aatgatttag atatcaaaaa catcactcct taacttataa cgttgaatat
     2461 ctagtagcta caactcttcc agctgcatca tatccccttt ggtaataaaa tccaatccag
     2521 ccattaccat ttttgtccca ttcttttgca actgcattcc ctggagaagc acctttttta
     2581 gtccaacttg cacctgttaa caaagcacca attgcccaac ctgggatacc aaacatagaa
     2641 gtgctagctt tccctcctgc ccaaaaggca gcattaatag cattagcagc tactccagtg
     2701 gttacagcaa tatttgtata ttcctttttg ccccatcttg caactgcagc tcttgtcatg
     2761 atatctgaag aatttgcaaa atcttccgca ctataaccat cctcatattt agttacgacc
     2821 ataccatctt cagtgtactc aataactaca ttttcagtag cattgttttc gtttaatgat
     2881 accacttggg aattttctga agcataaact atagtagttg acggtaaaac aactgcccca
     2941 atagttaaca aagctacccc taaagtaaat tttttcatag aatttcctcc tgatacagaa
     3001 aaatatatag aaaacgtttt ctttgtgaaa atcataaaac aaaaggtata ggcttgtcaa
     3061 tattttattt agtaaagata atttatattt ttgataatgt ggtatagtta gaatgcatta
     3121 ggaaaatatg ttatattaaa ttcttgttga cataatgtac actatgccaa gtagcccttg
     3181 tatcaaccga tacagaggct cactttgtga ttttcgattg gtctaggtca aaagctcttt
     3241 ttaagcttga ttttctgcgg ttgcttcttt ccgtttcttt gtgaattttt accagtcatt
     3301 ttttactggg ttgttatttt taataaaata tgataaattt ttggtagcgc ttcataagcg
     3361 cgttgctttt tcaagaataa acactcatta ttttttaact ggctgctcga aagagcagtc
     3421 ttttttatta tttatctgtt tcttttacaa acaattcttc gttaggactg ttgtaataat
     3481 ttggattcaa tttcaattca ccatgtttat tccctttatt gatcactgac aataacatcg
     3541 gactgaatcc tagaattcct agtaaaatag aaccgaatag aagatttttt cttgtcatga
     3601 atgttccctc cagttatttc caaagttgcc accatttttt ttcagtatca atttgtttgt
     3661 ctattgtttc tgttgcttca gaaagagtcg ttgagtcatt ctctggagct tcttctgtat
     3721 aagtcaactg tagtttttct tgcagttttt cgatttgttt gttggcttgt agggtgagtt
     3781 gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctctg agggcaagtt
     3841 gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt gtaaccacgt
     3901 ttttgcttgg tatattaaca tttttatcgt tgtcttgttc gtactcgtgc aacctatcta
     3961 gcaacatttg gttgcctaag ttgcgagcaa gttgcaatac tccagaacgc aatacaacct
     4021 tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata tgtttcgata
     4081 ttgcttgcct actcatctta aaaagttctg acaattcctt tattgtaaag gtttcttgtg
     4141 gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat cagataataa
     4201 actttttttc tcttttttcg aaagtgcgga ctctgtttgt ttttcaagag cttctttctc
     4261 tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct gtttgatttt
     4321 ttcgttaatt gttgtagcag gttcttcgta ttctttttgt ttatcagcta aagccaatgc
     4381 tttattttgt tcttctttgg ttggtgcatg tttattaaaa tcatctgctt ttttgatttc
     4441 tggtttaaac gtaaattgat agccaacgac aggttttcct cgcccttttc cgtatttctt
     4501 tttgattgat aatcctttga agattgcggt taattcctgt cgtataggct ttataacacg
     4561 attatcaacg tttgcttggt tgttccagta actttttgga atatctaata gttcatgaaa
     4621 atcctcttta gaaaaataag caaaaccttt agttcgatat tgttttagta atcgaaacat
     4681 agtctttgca tagctacttt gtaattcgtt aaattgttgg agtgaatagc gaacccactc
     4741 atctaaattg tttagtaaag gtaaagcttt ttcgtaaact tgtatgtcta catatggatc
     4801 tttagcagtt ccacgtattt taaactcact aaacataacg aatctatcta tatcgagccc
     4861 attagcactt cttttaccaa aacgtaaatc cattaattta tcataagtca tttctaaatc
     4921 attaatgaaa cgattattag cagttgcttt ataattactt aaatctttta gttggtcaaa
     4981 agtaagacgg actgttttat cgcctttttc gcgcattcta gacacaatag agaaaaatag
     5041 attcatttca actggtgtaa atttccgcaa cggaatggta tttaattcat gatggtattt
     5101 cacgatttca tttgccaaag atattcctcc ttactccttc aatggataca gacgttataa
     5161 acaacgtttt gtctgtgata atgcatatta taacatacgt gagtgcaaaa aaagagttta
     5221 aaagcagaca aaatgtagtt taaaagcaga caaaatgtag tttaaaagca gacaaaatgt
     5281 agtttaaaag cagacaaaat gtagtttata aaacgctgta tccattgaag gagtaaggac
     5341 acagccgacg gtaaagaggt ttaaaaaaag gtttaaaaag agattataaa agagggtgac
     5401 aaaatttccc gcctaaaaac cacttctttt ttctaccaac aggttcgcgc atttttcaaa
     5461 actgccaatt caaaaccttg ctccctacgg tcgctaaaaa agtgtgatcg ctatgctctc
     5521 acattgtact gcgcagtcgc ctaaaggctc ctttgctaaa aacaagtcag taacggaggt
     5581 gggggaagcc tctgatagct cgtctaagtc gttttaagac agtcttgggt aaaaaacctt
     5641 atcttgttct taaagcgcct tacaggcggt cagggcaagc cctaacaacc cgaaaataag
     5701 ggatacttaa aaacaccaca cagattttat tttgtaaggt ataataactt gtcgcctatc
     5761 cgacagtctt gcgactggaa ggaggtgtat ctaatgtttg aacggttcat ttgccccctt
     5821 cttgtagaag tttgtgtctc tttattcagt tattggttga ataaccgaaa caagaagtaa
     5881 aatcaataaa ggcgacatca acctgaaaaa aagccccaga gaatccgacc tctctggggc
     5941 tttcgtgtat ctaatgtttg atcatttgca ggttcagttt aacatgattg ctaatcaaat
     6001 tcaattttgt tgttgctgat ccaaccattc cttgacactt tctagataat ttaatgccgt
     6061 ttcttttttc gttggatttt gtatgtttgt tgcttgctga agcagtgttt cataacgttc
     6121 gtatgtgacc gattgttgcc gttcttttcg ttcttgttca ctggttgaaa aagggattgt
     6181 atcgctaaat aagttacaac aagcttcttc tgtctgcgta tctttaaaat attgcacgtc
     6241 tttttttact tgctcatatt tttcttgtac ctctttttgc tgtttttctg gtaagttttg
     6301 ata
//
