LOCUS       NZ_CP066259             4974 bp    DNA     linear   CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain CRKP78R plasmid p6.
ACCESSION   NZ_CP066259
VERSION     NZ_CP066259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17074911
            Assembly: GCF_016403085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   He,F.
  TITLE     Plasmid-borne tetA gene is an important factor causing tigecycline
            resistance in carbapenem resistant Klebsiella pneumoniae ST11
            clinical strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2020) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 23:30:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,895
            CDSs (total)                      :: 5,774
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 81 of 186
            Pseudo Genes (incomplete)         :: 113 of 186
            Pseudo Genes (internal stop)      :: 24 of 186
            Pseudo Genes (multiple problems)  :: 32 of 186
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066259.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP78R"
                     /isolation_source="urine sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2018-05-23"
                     /collected_by="Fang He"
     gene            <1..2412
                     /locus_tag="JCR35_RS29265"
                     /old_locus_tag="JCR35_29255"
     CDS             <1..2412
                     /locus_tag="JCR35_RS29265"
                     /old_locus_tag="JCR35_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_198804639.1"
                     /translation="VALAQLLRQRGVKPGDSVAVALPRSVFLTLALHGIVEAGAAWLPL
                     DTGYPDDRLRMMLEDARPSLLIATEDQLARFSDIPGLESLCYQQPLAAGDDAPLALSKP
                     DHTAYIIFTSGSTGRPKGVMVGQTAIVNRLLWMQDRYPLSADDVVAQKTPCSFDVSVWE
                     FWWPFIAGAQLVMAEPEAHRDPQAMQQFFARYGVTTTHFVPSMLAAFVASLDADSVAAC
                     RTLRRVFCSGEALPTELCREWERLTGAPLHNLYGPTEAAVDVSWYPACGPELAAVTGSS
                     VPIGWPVWNTGLRILDAAMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAP
                     GERMYRTGDVARWLANGAVEYLGRSDDQLKIRGQRIELGEIDRVMSALPDVGQAVSHAC
                     VFNQAAATGGDARQLVGYLVSDSGLPLDTAALKARLAEQLPPHMVPVVLMQLAELPLSA
                     NGKLDRKALPLPTLGGERSGRPPEPGMETLVAAVFSQLLGCEVNDIDADFFALGGHSLL
                     AMRLAAQLSRQLARQVTPGQVMVASTVGKLSALLAADLSDEQARRLGLDTLLPLRESDG
                     PTLFCFHPASGFAWQFSVLARYLSPRWSITGIQSPRPQGPMASAASLDEVCEHHLQTLL
                     AQQPHGPYYLFGYSLGGTLAQGIAARLRQRGEAVAFLGLLDTWPPETQNWAEKEANGLD
                     PEVLAEIDREREAFLAAQQGQASGELFSAIEGNYADAVRLLTTAHSAKFDGKATLFVAE
                     KTRQEGMDPQVVWGPWVGELEVFSQNCAHVDIISPQAFEAIGPVVREILG"
                     /name="amino acid adenylation domain-containing protein"
     gene            complement(2477..3271)
                     /gene="fepC"
                     /locus_tag="JCR35_RS29270"
                     /old_locus_tag="JCR35_29260"
     CDS             complement(2477..3271)
                     /gene="fepC"
                     /locus_tag="JCR35_RS29270"
                     /old_locus_tag="JCR35_29260"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225741.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="WP_002893737.1"
                     /translation="MSAVPSRLRGEQLTLAYGNKTIAESLNVTIPDGHFTAIIGPNGCG
                     KSTLLRTLSRLMTPASGHVWLDGAQIQQYASKEVARRIGLLAQNATTPGDITVQELVAR
                     GRYPHQPLFTRWRKEDEEAVNKAMRATGISDLALQSVDTLSGGQRQRAWIAMVLAQETA
                     IMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYATHLIALRDGRI
                     VAEGAPKEIVTADLIERIYGLRCTIIEDPVAHTPLVVPLGRR"
                     /name="fepC"
     gene            complement(3268..4260)
                     /gene="fepG"
                     /locus_tag="JCR35_RS29275"
                     /old_locus_tag="JCR35_29265"
     CDS             complement(3268..4260)
                     /gene="fepG"
                     /locus_tag="JCR35_RS29275"
                     /old_locus_tag="JCR35_29265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225742.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="WP_004147521.1"
                     /translation="MIAPSRRLIASCLLLMAASLLISLLGLAQGPVPLTIDQVFSALFG
                     DAPRNVAMVVNEWRLPRVLMALLIGAALGVSGAIFQSLTRNPLGSPDVMGFNTGAWSGV
                     LVAMVLFGQNLTAIALAAMAGGVLTSLVVWLLAWRNGIETFRLIIIGIGVRAMLVAFNT
                     WLLLRASLETALSAGLWNAGSLNGLTWGKTWPSAPLILLMLVGSALLVRRMRLLEMGDD
                     TACALGVQVERSRLLLMLVAVVLTAASTALAGPISFIALVAPHIARRLSGTARWGLTQS
                     ALCGALLLALADYGAQRLFMPWQLPVGVLTVSLGGIYLIALLIQESRKK"
                     /name="fepG"
     gene            complement(4257..>4974)
                     /gene="fepD"
                     /locus_tag="JCR35_RS29280"
                     /old_locus_tag="JCR35_29270"
     CDS             complement(4257..>4974)
                     /gene="fepD"
                     /locus_tag="JCR35_RS29280"
                     /old_locus_tag="JCR35_29270"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225743.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="WP_151381599.1"
                     /translation="NSGASFAIVLGAALFGITSPQEQLLLAFCGALCASLLVAFTGSQG
                     GGQLSPVRLTLAGVALAAVLEGLSNGIALLNPDVYDQLRFWQAGSLDIRTLQTLKIVLL
                     PVVVAGIAALLLSRALNSLSLGNDTATALGSRVARTQLIGLIVITVLCGSATAVVGPIA
                     FIGLMMPHMARWLVGADHRWSLPVTLLATPALLLFADVIGRLLVPGELRVSVVCAFLGA
                     PVLIWLVRRQPRGGGL"
                     /name="fepD"
     BGC             1..4974
                     /name="NRPS-like"
                     /gene="NRPS-like"
                     /tool="antismash"
ORIGIN
        1 gtggcgctcg cgcagctgct gcgtcagcgc ggcgtgaagc ctggggacag cgtggcggtg
       61 gccctgccgc gctcggtgtt cctgaccctg gcgctgcacg gcattgtcga agcgggggcc
      121 gcctggctgc cgctggacac cggctacccg gacgaccgcc tgcggatgat gctggaagac
      181 gcccggccgt cgctgctgat cgccacggag gatcaactgg cccgcttcag cgatattccg
      241 gggctggaaa gcctctgcta ccagcagccg ctggccgccg gggatgacgc gccgctggcg
      301 ctgtcaaaac cagaccatac cgcctacatc atctttacct ccggctccac cgggcggccg
      361 aaaggggtga tggtcggcca gaccgccatc gtcaaccgcc tgctgtggat gcaggatcgc
      421 tatccgttat ctgccgacga cgtggtggcg cagaaaacgc cgtgcagttt cgacgtgtcg
      481 gtgtgggaat tctggtggcc gtttatcgcc ggcgcgcagc tggtgatggc cgagccggag
      541 gcgcaccgcg acccgcaggc gatgcagcag ttcttcgccc gctacggcgt gaccaccacc
      601 cactttgtgc cgtcgatgct ggcggcgttt gtcgcctcgc tggatgccga cagcgtcgct
      661 gcctgccgca cgctgcggcg ggtattctgc agcggcgaag cgctgccgac ggaattgtgc
      721 cgcgagtggg agcgcttgac cggcgcgccg ctgcataacc tgtacggccc gacggaggcg
      781 gcggtggacg tcagctggta tccggcctgc ggccctgagc tggcggccgt gaccggcagc
      841 agcgtgccga ttggctggcc ggtgtggaac accgggctgc gcattctcga tgccgcgatg
      901 cgcccggtac cgccgggggt ggcgggggat ctttatctca ccggcatcca gctggcgcag
      961 ggctatctgg ggcgcccgga tctcaccgcc agccgcttta tcgccgaccc gtttgccccc
     1021 ggcgagcgga tgtatcgcac cggcgacgtg gcgcgctggc tggcgaacgg cgcggtggag
     1081 taccttggcc gcagcgacga tcagctgaag atccgcgggc agcgcatcga gcttggcgag
     1141 attgatcggg tgatgtcggc cctgccggac gtggggcagg cggtgagtca cgcctgcgtc
     1201 ttcaaccagg cggcggcgac cggcggcgat gcccgacagc tggtgggcta tctggtctct
     1261 gactccggcc tgccgctgga taccgcggcg ctgaaagcgc ggctggcgga acagctgccg
     1321 ccgcatatgg tgccggtggt gctgatgcag ctcgctgagc tgccgctgag cgccaacggc
     1381 aaactggacc gcaaagcgct gccgctgccg accctcggcg gcgagcgcag cggtcggccg
     1441 ccggagcccg gcatggaaac tcttgtcgcc gccgtcttca gccagctgct gggctgcgag
     1501 gtgaacgata tcgacgccga tttcttcgcc cttggcggtc attcgctact ggcgatgcgc
     1561 ctggcggcac agctcagccg ccagctggcg cggcaggtga ccccggggca ggtgatggtg
     1621 gcctcgacgg tgggcaaact gagcgcgctg ctggccgccg atctcagcga tgagcaggcc
     1681 cggcgcctgg ggctggatac gcttctgccg ctgcgcgaga gcgacggtcc gacgctgttc
     1741 tgcttccatc cggcctccgg cttcgcctgg cagttcagcg tgctggcgcg ctatctcagc
     1801 ccgcgctggt caattaccgg tattcagtcg ccgcgcccgc aggggccgat ggccagcgcc
     1861 gccagcctcg acgaggtctg cgagcatcat ctgcaaacgc tgttggcgca gcagccgcac
     1921 ggcccttatt acctgttcgg gtattcgttg ggcggcacct tagcccaggg gattgccgcc
     1981 cgcttgcgcc agcgcggcga agcggtggcc ttcctcggcc tgctggatac ctggccgccg
     2041 gagacgcaaa actgggcgga gaaagaggcc aacgggctgg atcccgaggt actggcggag
     2101 atcgaccgcg agcgcgaagc gttcctcgcc gcccagcagg ggcaagcctc cggcgagctg
     2161 ttcagcgcca tcgaaggcaa ctatgccgat gcggtgcggc tgttaaccac cgcgcatagc
     2221 gcgaagtttg acggcaaggc gacgctgttc gtggcggaga agacgcggca ggaggggatg
     2281 gatccgcagg tcgtatgggg cccgtgggtg ggggaactgg aggtgttcag ccagaactgc
     2341 gcccacgtcg atatcatctc cccgcaggcc tttgaggcca tcggtccggt ggtcagggag
     2401 attctggggt aacggttgag ttcgggctac agcaggtagc ccggctcagc gtagcgcaag
     2461 ccggggaaag aggagattac cggcggccca gcgggaccac cagcggcgta tgcgccaccg
     2521 gatcttcaat gatcgtgcag cgcagaccgt aaatccgttc aatcagatcg gcggtgacga
     2581 tctccttcgg cgccccctcg gcgacgatcc tgccgtcgcg cagggcgatc aggtgggtgg
     2641 cgtagcggca ggcctggtta agatcgtgca gcaccgccgc cagggtatag cccttctcgc
     2701 gattcagctc actcaacagt tccagcaggt cgatctgatg gctgatgtcc agccaggtgg
     2761 tcggctcatc cagcagcata atcgccgttt cctgggccag caccatcgca atccacgccc
     2821 gctgccgctg gccgccggaa agcgtgtcga cgctctgcag cgccagatcg ctgatcccgg
     2881 tcgctcgcat cgccttgttc accgcctctt catcttcttt gcgccagcgg gtaaacagcg
     2941 gctggtgcgg atagcgcccg cgggccacca gctcctgcac cgtgatatcg ccaggggtgg
     3001 tggcattctg cgccagcagc ccgatgcggc gagcaacctc tttactggcg tactgctgga
     3061 tctgcgcgcc gtcgagccag acgtggccgc tggcgggcgt catcaggcga ctgagggtgc
     3121 gcaatagcgt cgacttgccg cagccgttgg ggccgataat ggcggtgaag tgaccgtcag
     3181 ggatggtcac gttcagcgac tcggcgatgg tcttgttccc gtaggccagg gttaactgct
     3241 cgccgcgcaa acgggagggg acagcactca ttttttgcgg gactcctgaa tcaacaatgc
     3301 aataaggtaa ataccgccga ggctgacggt taacacgcca accggcagct gccacggcat
     3361 aaacagccgt tgggcgccgt aatcggccag cgccagcagc agcgcgccgc acagggccga
     3421 ctgggtcagt ccccagcggg ccgtgccgct caggcggcgg gcgatatgcg gcgccaccag
     3481 cgcgataaat gagatcggcc cggccagcgc cgtcgaggcg gcggtgagca ccaccgccac
     3541 cagcatcagc agcaggcgcg aacgctcgac ctgcaccccc agcgcgcagg cggtatcgtc
     3601 gcccatctcc agcagccgca tccggcgaac cagcagggcg ctgcccacca gcatcaacag
     3661 gatcagcggc gccgacggcc aggttttacc ccaggtcagg ccgttaagcg atccggcgtt
     3721 ccacaggccc gcagaaaggg cggtctccag cgaggcgcgc agcagcagcc aggtgttgaa
     3781 tgccaccagc atggcgcgca cgccgatgcc gataatgatc aggcggaagg tttcgatccc
     3841 gttgcgccag gccagcagcc agaccaccag cgaggtcagc accccgccgg ccatcgccgc
     3901 cagggcgatc gccgtcaggt tctggccgaa gaggaccatt gccaccagca cgccgctcca
     3961 ggcgccggtg ttaaagccca tcacgtccgg gctgccgagc gggttacggg tcagcgactg
     4021 gaaaatggcg ccgctgacgc cgagcgcggc gccgatgagc agcgccatta acacccgcgg
     4081 cagccgccat tcgttcacca ccatcgcgac gttgcgcggc gcatcgccga acagcgccga
     4141 gaacacctgg tcaatcgtca ggggcaccgg gccttgcgcc agcccgagca gggaaatcag
     4201 caggctggcg gccattagca gcagacagct ggctatcagg cggcgggacg gggcaatcac
     4261 aggccacctc cccgcggctg gcggcgcacc agccagatca gcaccggcgc gccgagaaag
     4321 gcgcacacca ccgatacccg cagttcgccg ggcaccagca ggcggccaat cacgtcggca
     4381 aacagcagca gggccggggt ggcgagcagg gtgaccggca gcgaccagcg atgatcggcc
     4441 cccaccagcc agcgcgccat atgcggcatc atcaggccga taaaggcgat ggggccgacg
     4501 acggcggtgg cgctgccgca caggacggtg atgacgatca ggccgatcag ctgggtgcgg
     4561 gcgacgcggc tgccgagggc ggtggcggta tcgttgccca ggctcaggct gttcagcgcc
     4621 cggctcagca gcagagcggc gatccccgcc accactaccg gcagcaggac aattttcagc
     4681 gtttgcaggg tgcggatatc cagcgaaccg gcctgccaga agcgtagctg atcgtagacg
     4741 tccgggttga gcagggcgat gccgttggac agcccctcga gtaccgccgc aagggcgacc
     4801 ccggcgagag tgaggcgcac cgggctcagc tgaccgccgc cctggctgcc ggtaaaggct
     4861 accagcagcg aggcgcagag cgcgccgcag aatgccagca gcagctgctc ctgcggggaa
     4921 gtgatcccaa acagcgccgc gccgaggacg atggcgaagc tggcgccgga gttg
//
