LOCUS       NZ_CP066216             9392 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Enterococcus faecium strain 18-042 plasmid p18-042_1, complete
            sequence.
ACCESSION   NZ_CP066216
VERSION     NZ_CP066216.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16988095
            Assembly: GCF_018517185.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Moreaux,J., Zouari,A., Potrel,S.,
            Collet,A., Lecourt,M., Auger,G. and Cattoir,V.
  TITLE     Genetic features of the poxtA linezolid resistance gene in human
            enterococci from France
  JOURNAL   J Antimicrob Chemother 76 (8), 1978-1985 (2021)
   PUBMED   33895846
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Zouari,A., Potrel,S., Collet,A. and
            Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2020) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 05:03:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,615
            CDSs (total)                      :: 2,524
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 25 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066216.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="18-042"
                     /isolation_source="Digestive"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p18-042_1"
                     /geo_loc_name="France: Tourcoing"
                     /lat_lon="50.7239 N 3.1612 W"
                     /collection_date="2018-01"
                     /collected_by="NRC for Enterococci"
     gene            60..341
                     /locus_tag="I8F50_RS12905"
     CDS             60..341
                     /locus_tag="I8F50_RS12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353845.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIENDF
                     DHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            343..759
                     /locus_tag="I8F50_RS12910"
                     /old_locus_tag="I8F50_12910"
     CDS             343..759
                     /locus_tag="I8F50_RS12910"
                     /old_locus_tag="I8F50_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032495245.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTITSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1261..1878)
                     /locus_tag="I8F50_RS12915"
                     /old_locus_tag="I8F50_12915"
     CDS             complement(1261..1878)
                     /locus_tag="I8F50_RS12915"
                     /old_locus_tag="I8F50_12915"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_214297225.1"
                     /translation="MFPKNQCYLDKNIYSDLKWAEDIIGHFNSFFYSNQSPTFKHHHIK
                     KNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCYDNPKYKKAIRSWANVIRAYR
                     NKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQKLYLYGGLYVIKHLLIYEDSM
                     TQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKLKIMIV"
                     /name="Abi family protein"
     gene            1978..2658
                     /locus_tag="I8F50_RS12920"
                     /old_locus_tag="I8F50_12920"
     CDS             1978..2658
                     /locus_tag="I8F50_RS12920"
                     /old_locus_tag="I8F50_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_002325565.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRDINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            3617..5245
                     /gene="poxtA"
                     /locus_tag="I8F50_RS12925"
                     /old_locus_tag="I8F50_12925"
     ARG             3618..5242
                     /name="poxtA"
                     /gene="poxtA"
                     /gene_name="ABC-F type ribosomal protection protein PoxtA"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL/OXAZOLIDINONE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5299..5979
                     /locus_tag="I8F50_RS12930"
                     /old_locus_tag="I8F50_12930"
     CDS             5299..5979
                     /locus_tag="I8F50_RS12930"
                     /old_locus_tag="I8F50_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_214297223.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRDINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NFIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(6002..6418)
                     /locus_tag="I8F50_RS12935"
                     /old_locus_tag="I8F50_12935"
     CDS             complement(6002..6418)
                     /locus_tag="I8F50_RS12935"
                     /old_locus_tag="I8F50_12935"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_231433844.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQEM
                     IKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSFIR
                     NQLHYFVNQIEIFWKKSLSDNMCVWFCCKVLNKE"
                     /name="Abi family protein"
     gene            7567..8535
                     /locus_tag="I8F50_RS12940"
                     /old_locus_tag="I8F50_12940"
     CDS             7567..8535
                     /locus_tag="I8F50_RS12940"
                     /old_locus_tag="I8F50_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002332718.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIAYFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            8552..9076
                     /locus_tag="I8F50_RS12945"
                     /old_locus_tag="I8F50_12945"
     CDS             8552..9076
                     /locus_tag="I8F50_RS12945"
                     /old_locus_tag="I8F50_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002305828.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     Typing          7568..8531
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.764"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caaatactat atttgtatga tatacataaa ttatacactg atacgaaagg aaaatttcca
       61 tggacatttt agttcgaaat atcaatccca aaattattac aagaatagat aaacgttgtc
      121 aggaactaac aaccaaaacg ggcaaaaaat ggagtcgaaa tcaataccta aaaattttaa
      181 tcgaaaacga ttttgaccac gaacttatga attacaaaaa agatcaattc gatttattat
      241 tcgaaaaatt tattgccatt caagcagaaa atacgaacat actaaatgac tatgttcaca
      301 caaacaacca actcattgaa acacttatcg gaggaatata acatgagaaa aacttctgtt
      361 caaaaacacg taacccttta tcccagtcac gttgacgttt tagattccat catcgaaaaa
      421 aatccagacg ttaacaatta ttcagaagcc attcgctttt tatgtatgag ttatgaacct
      481 gataccaata caaccataac cagtaaccaa gcaaaactca atgccatggg aaaagaaatt
      541 tctatcatga cagaaatcat ggcagaattt gctgatttat atctaaaaga tactggtgtt
      601 cttatcggta caaattctaa agtatatcaa gaagtgaaag aaaaaattga aaatcaactc
      661 aatccttccc gacgaaatca acccaaaaat cgtcaaaaag aaaccaatca aacctttact
      721 tctggaaatt ttgacccaac aaactttcta aaaagataaa aaaagaaacc gaaaaacaaa
      781 aaaattcctc gatttctttt tagggatcgg tcttccagca agatttgcaa gttggtacca
      841 acttgcgaaa aaaaagaaga attcgcagaa gcggattttt cttttttcat tttggggcgc
      901 tgccctaatc cctaagtccc ggttattcaa tagcatggaa actcaaagaa taggacgttc
      961 cctggaataa aaaaaggctg ctcgttcgag cagtccatac ataggaaaat gaaaaaacga
     1021 cgtcccttac tgagaaacgc tattaaactt ttaacaaatc cctcggaaaa taacaagtag
     1081 taaaaaaata atcactgaat attcacaaag aatcgaaaaa agccaacagc gaagaatcaa
     1141 gcttttaaaa agatgtttta gatagaccaa ttgaaaatca caaagtcttc ccctggatcg
     1201 gttgatccag gggctacttg gtagtatgta cattatgtct actaacaatt taaataacaa
     1261 ttaaacaatc attattttta acttgtttct ccaattttcc ggaaaaccat aattggattt
     1321 ttctaaggaa gggattttat cgatcttttt ttctaaatct attagaaatt tattccataa
     1381 agtttgtgtc atgctatctt catatattaa cagatgtttt ataacgtata atcctccata
     1441 caaatataac ttttgggttt cttttaagtt attttcagga aaataacttt tggaatcatt
     1501 ttttatgatt gttggagcgt cagtaacttt aaatgcatat attcttgaat gatgagctat
     1561 cttatttcta taagctctaa ttacgtttgc ccaacttcgt atagcttttt tgtatttagg
     1621 attatcataa cagctcatga cccaattatt ataatactct gtattaagtt gattaataaa
     1681 tgttgttaaa ctaccaaatg ttatttcttc aaataaagcc catattggta aacaatgatt
     1741 tttttttata tgatgatgtt taaaagtggg actttgattt gaataaaaaa aactattaaa
     1801 atgaccaatt atatcttcag cccatttcaa atctgaataa atatttttat ccaaataaca
     1861 ctgattcttc ggaaatatgc tagtttcttc ataagaaacg cacggttctg ttgcaaagtt
     1921 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
     1981 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggctactat
     2041 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tgacattaac
     2101 gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact ctatcaaatt
     2161 tggaaaaaga aaaataaaaa atccttttat tcatggaaaa tggatgaaac gtacatcaaa
     2221 attaaaggaa aatggcatta tttgtatcga gccatcgatg cagatggttt aaccttggat
     2281 atttggttac gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa gcggttagtg
     2341 aagcagtttg atgaaccgaa ggttgtagtc acagataaag ccccctctat tacaagtgcc
     2401 tttaagaaac taaaagaata cggcttttat caagggacag aacatcgtac cattaaatac
     2461 ctgaataatt tgattgaaca agaccatcgt ccagtaaaga gacgcaataa attctatcga
     2521 agtttacgca ctgcctctac cacgattaaa ggcatggaag ccattcgagg attatataag
     2581 aaaacccgaa aagaaggcac tctcttcggg ttttcggtct gtactgaaat caaggtatta
     2641 ttgggaatcc cagcttaaat catagatacc gtaagggatt ttattcttta tttaaaactt
     2701 tgcaacagaa ccacatgaca aatgcaagct ttggaattta cattattcat tatcctgttg
     2761 ttgtgtgggt atgctacttg ctttacagtt acctcaactt accgatgata tttatctata
     2821 ttcttgcttt agggcttgaa ctgatactga caccgttaat ttatgagctg tttaaaagga
     2881 ttcctgtagt tcgcttttta gtattgggga taaaaaaatg agcatttctt actatcctat
     2941 taaacgagta tagctatcct atcggcagta gcgttgtcta tggttatggc ttgcctgcat
     3001 attaaaaaag ggcaaaagaa aaacagagga ttttacccct ctgtttcctc ttttgcgtta
     3061 catattcagt ttacagttgt acaatacttg gtctataaac aaactgtaaa gtgagcagct
     3121 tagatgagct attgacatat gcactcaagc tctctataat taaaatataa aataagagag
     3181 ggtgagcaca gtgttattcg ggaagttttg cattgatttt ggctctacac aacaatataa
     3241 gacagagcat tgtgtggagc ccaatcagat aaaatgttta tttgagaggc agggctattt
     3301 aaatcagaaa tttgtgattt aagagcctta tttgtgttgc ctttcaataa gcaaactcaa
     3361 tgattggata tgtggccgta gacaatgttt tgtctacggc cttttgctgt ctatatttca
     3421 tctatgttat tggcatagtg tctgaaaaag cagagggcta cgtctatggg acgtattgct
     3481 acctctgctt ttatttttta aatgatgata tcagagaaga aaatatatcc ccgcacagga
     3541 gataatcaag caaggaaaac tgtaagccat aaaatgatgg cttgcagtat gtatgacgat
     3601 ggaggaatga tacaagatga aaggtaaaaa tatgaattta gcctttgggt tggaagaaat
     3661 ttatgaggat gctgagtttc aaatcggaga tttggataag gtcggtattg tcggcgtgaa
     3721 cggagccgga aagaccacct tgttccgcct gctgttggga gaacttgaac ttgataatgg
     3781 ttcactgacc agtggaaatg cccgtattgg ttatctccca caggaaattg tcttggaaga
     3841 tgaggatatt accgtttggg atttcctttt tgagggacgt ccgattaaaa agtatgagca
     3901 ggaattggaa gaaatctata aaaagcttga aaccgcagtc aatgcagagc aggaagcact
     3961 gcttgcccga atgggaacat tgcaagaacg cttggagtat ttcgacttct atgaggcaga
     4021 aacaattctg ttggagtttg cagataaaat gagcattgat gcagaattat atcatcgtcc
     4081 gatgagagag ctttcaggcg gacaaaaatc caaaatggca tttgccagac tactatattc
     4141 aaaaccggaa attctattgt tggatgagcc taccaaccat ttagatgtca gcacaaagga
     4201 ttttgttata aaatacttaa agaattatag gggttcggta ctgattatca gccatgatat
     4261 tgattttcta aatcggatta tcaacaaaat tatgtacatc aacaaagcta cccataaaat
     4321 atctgtttat gatggagact actacatcta caagaaaaag tatgcagagg aacagcggat
     4381 tcgtgaaatg gcgattgtac agcaggaaaa agaaataaag gagctttccg attttgtaca
     4441 aaaagcaaaa caagccagtc agaccaatca tcacctcaaa cgaatgggtc aagagcgagc
     4501 cttgcggctt gataaaaagc gtggagagct gcaaaagaga aatcgactgt acaagcgtgt
     4561 gaagatggat attcgcccca agcgtgaagg ggcacaagtt cccttagagg tggaaaatat
     4621 caccttccac tattcggggt atcccaccct ttatcagaac ctttcctttc agattaacgg
     4681 aagagaacga tttcttgtgg tgggtgaaaa cggtgtcggt aaatccacct tattgaaatt
     4741 gatgatgggt attctcagtc cagatgaagg atgcattcgc tttaaccaga aaactgatat
     4801 tgcatattat gcacaggaac tcgaacagct tgatgaaaac aaaacggtca ttgacaatgt
     4861 ggagtctgaa ggatatacac cgtggcaaat cagagccgta ctgagcaact tcctgtttta
     4921 tgatgacgat gtaaacaaga aagtatctgt gctgtcccct ggagaaaaag caagggttgc
     4981 cctttgcaaa atcctattac agaaagccaa tcttttgata ctggacgagc cgaccaacca
     5041 ccttgaccca gaaacgcaga aaatcattgg cggcaacttc aatttgtttg agggaaccat
     5101 tattgccgtt agccataacc catcctttgt ggaacaaatc ggaattagcc gtatgcttat
     5161 tttgcccagc ggtcgaattg aaccctattc ccgtgagctg cttgagtatt attatgaaat
     5221 caacggttct gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag
     5281 atagcaggag gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt
     5341 attgtagccg tgggctacta tcttcgttat aaccttagct atcgtgaagt tcaagaaatc
     5401 ttatatgatc gtgacattaa cgtttctcat acgacgattt atcgttgggt gcaagaatat
     5461 ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa
     5521 atggatgaaa cgtacatcaa aattaaagga aaatggcatt atttgtatcg agccatcgat
     5581 gcagatggtt taaccttgga tatttggtta cgtaaaaaac gggacacaca agcagcctat
     5641 gcttttctta agcggttagt gaagcagttt gatgaaccga aggttgtagt cacagataaa
     5701 gccccctcta ttacaagtgc ctttaagaaa ctaaaagaat acggctttta tcaagggaca
     5761 gaacatcgta ccattaaata cctgaataat tttattgaac aagaccatcg tccagtaaag
     5821 agacgcaata aattctatcg aagtttacgc actgcctcac ccacgattaa aggcatggaa
     5881 gccatccgag gattatataa gaaaacccga aaagaaggca ctctcttcgg gttttcggtc
     5941 tgtactgaaa tcaaggtatt attgggaatc ccagcttaaa ttatagatac cgtaagggat
     6001 tttattcttt atttaaaact ttgcaacaga accaaacgca catattatct gaaagagatt
     6061 ttttccaaaa aatttctatt tgattcacaa aataatgcaa ttgattacga atgaatgaat
     6121 ctaatttgta cgtttcaata acatctagaa tagtaacttt atctatattt ttcattaatt
     6181 taacataaat actaaatcga aaataactat ttttctttaa aaacttttct aataaaattg
     6241 tatcggaatt tgttaaaaaa atatctttac ttattttttt tatcatttct tgctcggata
     6301 aatatggttt taactcttga ttaattactt tggaattatt aattacacat aatgcctttt
     6361 taggtggaat tctatgatta actgttttaa ctttatattt tctatttgat gtcttcataa
     6421 gaatcttctt tcttaaaaaa atataaaaaa aaagccctac cataatgcgc atgatattaa
     6481 atcagaggcg tggcaagtac tgatagatat attaaaacac atttgaatta aactgtaaag
     6541 cttttttatt taaaaattta gttgtcaaaa tcgtgatttt ttttaagtgt gcaaccaaaa
     6601 gaaatgccta tatatcaagc attactacca taatttttca cattattccc cccctgttag
     6661 aatgcggggg gctaaatgcg atcggtctat cgagcaagct cgatagacct ttttttgctt
     6721 ggaaatggcg caaaaccggt gcctttcacg aatttgtaag cttgtttgta cacgtctcgc
     6781 tctaggagac cttgttcgct ccgcaccgca agcagctttt agcaacagca cttttttgct
     6841 aacgtacgaa aatggctcct ccggaattct gctgaatcgg ttgcttattg ttttgtttca
     6901 tacgctctta tttgcgtttt cagacgtttt actacccaag gtatataaaa tatacaagaa
     6961 catagataag acagattaaa tcgaatttta ggatggtttt atgctcttta agggttgtta
     7021 gggcttgccc tgaccgcctg taaggtgctt taagaacaag ataagggtta tccatactaa
     7081 actgtcttaa aacggcttaa acgagcgcgc agagcctcta aaaaaatctc tttaaacaac
     7141 gtttttagca aaggagcctt taggcgactg cgcaggtaca atgtgagggt gtttttccct
     7201 cacacctttt tagcgaccag agggagcaag tttcccagtt tagtaaaatt cgcgaacctg
     7261 tttggctaaa aaagcagaaa atttttaggc gggaaatttt gcccctctcc tcttttataa
     7321 tctcttttta aacctctttt taaacctctt taccgtcggc tgtatgctta ctctcccaag
     7381 gtgtgcagaa tcttttgggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     7441 aggggtcttt ttgtctgttt ttgggggtct ttttgtctgt ttttaggggt ctttaattag
     7501 gggcttgacc cctattagaa agctctttat aattttgtta agtagttaat cttgaaggag
     7561 gattttatgg caaatgaaat tgtgaaatac caccatgaat taaatacgat tcctttacgc
     7621 aaatttacgt cagtagaaat gaatttattc ttctctatag tatctagaat gcgtgacgta
     7681 ggcgataaaa aagtacaatt tacattcgaa caattaaaag atttaagcaa ttataaagca
     7741 actgcaaatg ttcgattcat agatgattta gaaacaacgt atgacaaatt aatggattta
     7801 cgttttggta gaagaagtgc tgacggtttg caacgtgaac gatttgtttt atttaatcaa
     7861 tttaaaatcg atggaaaagc tgatattcct tttgctgaaa tacaagtcca tgagaaagcc
     7921 ttacccttac ttaataatct tgaagaatgg gttcgatatt ctctccaaca attcaatgaa
     7981 ttagaaagca gttattccaa aaccatgttt agattattaa aacaatacag aaccaaagga
     8041 attgcctatt tttcaaaaga agactttcat gaattacttg atatccctaa aagttataga
     8101 gaaagtgata taaataaaaa aattataaag cctattcgcc aagaactaac cgcaatcttc
     8161 aaaggtctga caatccaaaa gagatacgga aaaggacggg gaaagcctgt cattggctat
     8221 caatttacat ttaaacctga gataaaaaaa tcagacgact ttaacaaaca tgccccaaca
     8281 aaagaagaac aaaacaaagc attagaacta gctaaccaac aagttcaatc tgaaaaagca
     8341 cctacttcat tacaagaaaa aatcaaagaa caaaagcgaa aattggaact tttacaacag
     8401 ttagaaaagt tagaaagaga acaaaatacc caaaataaag aaaataatgc acaaaaatca
     8461 gagataaatt ccgatattcc ttcagaacta aaaaaagact tattagacaa tcttcaaaac
     8521 ttattcaaag attgaggggt ttttagattt tatgacagaa aaaacaatca aagaacttgc
     8581 tgaagaatta ggagttagta aaacagcaat taacaaaaaa gtttctgacg aaaatcgaaa
     8641 actttggttt tcgaaaatcg gaaacaagtt tgtgattaat gaaaccggac aaaaggctat
     8701 aaaatcaatg ttttttaacg aaaacgaaaa ctcaaatcaa caactagttg gcgaaaaaac
     8761 taaaaccgaa aaccacaaaa acgccaacca agttcccgaa ttagaagtcc tttctctcct
     8821 agaacaacag ttaaatcaaa aagatatgca actacaagaa aaagacgagc aaataaaaca
     8881 aatgcaaaaa ttactcgacc aacaacaaca actcaccctg caagccaacc aacaaatcga
     8941 gaaactgcag gaacaactac agttgactta tacggaagaa tcttcggaaa ataaatcaac
     9001 gactctttct gaagaaacag aatcgataga aaaacaatca aagacggaga aaaaatggtg
     9061 gcaattctgg caataaaacc agcaatttaa attaataaaa attgctggtt ttattcttct
     9121 tttttctttt gcgagcagag caacatgggc ccatgttgcg atattttggt ggcgccccaa
     9181 tccccgtttt ttcgttgaat cttactcgga ctgcactcgt tttacagctg atttttctga
     9241 attttgaaag agcataagga aacgtggtat cactttctta cgaaaggtgc tagataagct
     9301 gtgcctatca cgtttctcag aaaaaaattt gctatcactt ttttgccatt gtggctaggc
     9361 tatgtatagt gttttggcga aaaatagcta ta
//
