LOCUS       NZ_CP066155            11970 bp    DNA     circular CON 10-DEC-2023
DEFINITION  Klebsiella pneumoniae strain KP697 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP066155
VERSION     NZ_CP066155.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17023459
            Assembly: GCF_021497485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Li,X.
  TITLE     A novel KPC-2 variant conferring resistance to ceftazidime-avibactam
            in an strain of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Centre of Laboratory Medicine, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hang Zhou, Zhe
            Jiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/10/2023 02:30:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,958
            CDSs (total)                      :: 5,831
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 69 of 183
            Pseudo Genes (incomplete)         :: 121 of 183
            Pseudo Genes (internal stop)      :: 25 of 183
            Pseudo Genes (multiple problems)  :: 32 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066155.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP697"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="30.26 N 120.19 E"
                     /collection_date="2020-06-23"
                     /collected_by="Xi Li"
     gene            813..2777
                     /locus_tag="JDZ44_RS29595"
                     /old_locus_tag="JDZ44_29485"
     CDS             813..2777
                     /locus_tag="JDZ44_RS29595"
                     /old_locus_tag="JDZ44_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2777..3508
                     /gene="mobC"
                     /locus_tag="JDZ44_RS29600"
                     /old_locus_tag="JDZ44_29490"
     CDS             2777..3508
                     /gene="mobC"
                     /locus_tag="JDZ44_RS29600"
                     /old_locus_tag="JDZ44_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3515..4045
                     /locus_tag="JDZ44_RS29605"
                     /old_locus_tag="JDZ44_29495"
     CDS             3515..4045
                     /locus_tag="JDZ44_RS29605"
                     /old_locus_tag="JDZ44_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4072..4251)
                     /locus_tag="JDZ44_RS29610"
                     /old_locus_tag="JDZ44_29500"
     CDS             complement(4072..4251)
                     /locus_tag="JDZ44_RS29610"
                     /old_locus_tag="JDZ44_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4277..4705)
                     /locus_tag="JDZ44_RS29615"
                     /old_locus_tag="JDZ44_29505"
     CDS             complement(4277..4705)
                     /locus_tag="JDZ44_RS29615"
                     /old_locus_tag="JDZ44_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4869..5183)
                     /locus_tag="JDZ44_RS29620"
                     /old_locus_tag="JDZ44_29510"
     CDS             complement(4869..5183)
                     /locus_tag="JDZ44_RS29620"
                     /old_locus_tag="JDZ44_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5171..5449)
                     /locus_tag="JDZ44_RS29625"
                     /old_locus_tag="JDZ44_29515"
     CDS             complement(5171..5449)
                     /locus_tag="JDZ44_RS29625"
                     /old_locus_tag="JDZ44_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5624..5989)
                     /locus_tag="JDZ44_RS29630"
     CDS             complement(5624..5989)
                     /locus_tag="JDZ44_RS29630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5986..6357)
                     /gene="tolA"
                     /locus_tag="JDZ44_RS29635"
                     /old_locus_tag="JDZ44_29525"
     CDS             complement(5986..6357)
                     /gene="tolA"
                     /locus_tag="JDZ44_RS29635"
                     /old_locus_tag="JDZ44_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6631..6876)
                     /locus_tag="JDZ44_RS29640"
                     /old_locus_tag="JDZ44_29530"
     CDS             complement(6631..6876)
                     /locus_tag="JDZ44_RS29640"
                     /old_locus_tag="JDZ44_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7521..9008
                     /gene="ltrA"
                     /locus_tag="JDZ44_RS29645"
                     /old_locus_tag="JDZ44_29535"
     CDS             7521..9008
                     /gene="ltrA"
                     /locus_tag="JDZ44_RS29645"
                     /old_locus_tag="JDZ44_29535"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9113..10798
                     /locus_tag="JDZ44_RS29650"
                     /old_locus_tag="JDZ44_29540"
     CDS             9113..10798
                     /locus_tag="JDZ44_RS29650"
                     /old_locus_tag="JDZ44_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10808..11065
                     /locus_tag="JDZ44_RS29655"
                     /old_locus_tag="JDZ44_29545"
     CDS             10808..11065
                     /locus_tag="JDZ44_RS29655"
                     /old_locus_tag="JDZ44_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11150..11299
                     /locus_tag="JDZ44_RS29660"
                     /old_locus_tag="JDZ44_29550"
     CDS             11150..11299
                     /locus_tag="JDZ44_RS29660"
                     /old_locus_tag="JDZ44_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4113..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11403..11968)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.418"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2778..3505
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ttttgcctca catgttagtc ccctgcttat ccacggaatc tgtgggtaac tttgtatgtg
       61 tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg accgagtgag cgaggaggcg
      121 caatttcccc ggcacctgca agacggtcac cggacgcgct gatgtgggat attgtcccct
      181 gattaagtct ttctattccc tgacgatcaa ttatcaggat aggtgaacca tgtcggtttt
      241 tcgctgaaca ttttacgtta acctatctct gttgcttttt tctccttgcg gagaaatgca
      301 agtcatcccc gctggggcgt ggctgacttc aggggaaaag accttgctta cgctggctgg
      361 acgccaccga cgcacggtct tttcccctct ttctggctct gtccggctgg acgccggcca
      421 cgccagaaat tcacccggcc cccgtcaggc tcacgcctga ccacccacaa aaatccacgg
      481 tgctggacgc acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct
      541 cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg gggtgccgcc
      601 ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc ttttccgcat
      661 ggggcggaaa gaggctggcc tccgcggtga aagctgaacg caatcaccac ggacgtgacc
      721 gccgccgcca tgccttgggg catgtccgcg gcttgtgcag cgggggctgc gcatcacgcc
      781 ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa tacggattcg cttgcctggc
      841 agtggcatat cccggagctg gcccggcaga tttacccgtg ggtcagctgg ctgtaccggg
      901 agtattacag tctggccggt ttttttctgg ttatcggcct gtttttcggt ctggctgaac
      961 tctgggccgg ccgaatgcgt tttgtggggc tgagccataa ggcgcggcgg gagtatgccg
     1021 atcgctataa gcgcgataat aaccggactg tgccattttc ggtgctggcc tcgctggatt
     1081 cacccgggcg gatggtcagg gcacggcgac atgtctggaa ctacgcgtac tggctgaccg
     1141 gcgctgccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc gagagctggg
     1201 gagaaaccgt cctgcgcccg gacctctatg cgaacgcaga ttattacctg ctgcagccgg
     1261 ctaacctgtt cgggatagcc tggtgtgccg gagggcttgt cgccggcgtc cttctggcgc
     1321 ggctgctggc gttcctgctg cttgatggtc attttgcggc cgctgacgag gcggtgaacg
     1381 cgaaactgaa tcaggagagc cggcgcagca gccagcggac cggagaaatg acagacgtcc
     1441 gccacctgca tttcggggag ccggtaccgg ttaacgcgct ggccgatttt tccacggagc
     1501 aggcccggaa acagcaggcg gtgtttctgg gtaaggatga gcagggccag ccggtgctgg
     1561 tccctcgtga tacctggcgc aagaccaata ttcagatcct cggcctgccc gggagcggca
     1621 agagcgcgat ggccaccaac gcgctcattc gctgcgtacg cgatttcggg gacgctgtgg
     1681 tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg cactgcccgg
     1741 acttcaccct gctggatctg cggccgggga aaccggcgca gctgaatctg ttccgcgatc
     1801 tcgaccagta cgccctgaag aatctgctgg ttgccgggtt taacctgagt gagaccggcg
     1861 acgtggcgga ccactaccgc atcagcgagc agaaagcggc gaagctgatt gcggagcagt
     1921 tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc gtacgcgttg ccggaggccc
     1981 tgaaaaagga cgtgaagggg ctgatcacca agctggagaa cgtggccgac ctgagcgtcc
     2041 tgcagacgga cagcggtatc gatgtggcgg ggataatcaa cggcggcggc tgcctgtacg
     2101 tcatcggctc gatggacgat gaggcggtga tccgggtgca gaagatgctg tttgcccgct
     2161 gcgcgcaaat catcattgcg cgggatgagt tccggaggtg gccgcacgcg agcatcatgc
     2221 tcgatgagat taaatatctg ctgtcgaagt acgtgctgaa cgcgctgggg acgctgcgca
     2281 gccgggactg caacctgctg ctcgcgcacc agtcgctggg cgacttcggt cagtgcgggc
     2341 aggatctgcc cgctgatttt gtgaaaacca cggtactgga taacacgccg atccgctggt
     2401 tctatcgcgc ggccagccag gagtccgcgc agtgggcggc gggccagacc ggggagatcc
     2461 gggtggacgt ggagcgccgc cgggccagcc gggaggcggg gaacgtggag cacatcagcg
     2521 gcgacagctt catccagaag gaggcgcggc cgctgtttga cgtgaacacc ctgcaacacc
     2581 tgcctgacgg gttcgcggtg atgaccgggc tgggcgtggc gcggctggcg ttcagcagcc
     2641 cgctgcgggt tgagcgccgg gaaatttcgc tgaaaacctt cccggtgctg gcgaaaacgg
     2701 acccgctggc ggagtacctg cctccggccg acggcggcgg accaaaagcg gatgatgatt
     2761 ttgcggggct ttactgatga cactggagca gtacaacgtc agccacgcga agcggcaggc
     2821 gcggaacgcg gagaaaacac ggctgaccct gcgctggctg cgggaagagc tgtgttccac
     2881 ggcggagctg gttgcgcgac ggctggggat tgcggcggta cagccggttt accgcttcct
     2941 ggacagtctg gtggcgaaag ggctgctggt ccgggcgaaa tacccggttg acggccgtca
     3001 ggtcagcgtg tgggggctga cgccgcacgg ggtggcgttc tcgttcgacg aggacgagcc
     3061 gctgacggac gttattccgt ttcagccgtc aagggtgtcg gcggcgcagc tgccgcaccg
     3121 gctggccgtg cagtccctgc gcctctccat ggagggccgg ggggccaccg gctggcggta
     3181 cctgcaccgt atggccctga aagggatgaa agtgccggac gcgctggcgg agctggacgg
     3241 cagaacggtg gcatttgagg tagagcgcac cgtcaagagc cgcaggcggt atcaggaagt
     3301 ggtgtccggg tatctgttca accgccgggc gaacggcatc gatgaaatct ggtacatctg
     3361 ccctgaccgg ccgacgcttg tccgggtaca gcgggcgatt ctggcggtgg atgaaatcgt
     3421 gaatccacag accggcgagg cccgcaaaac ggtggagctg gaccgggaga ggctgttcgc
     3481 gtgctttaaa tttatgacaa cggagtgagg gattatggcg ctgtacatgg tatttattga
     3541 ggacgtggaa gacaaagacg gcgagcgctg cattatgcca accagcgagc atctggaaga
     3601 catggtggtg ctcgcctggc agaccggggc gtatgttttc tgttttgtcg accgggccca
     3661 gcatctggcg atgatgaaat ccatcgggat gtatgacgag cggctcaaca gcgacgaaaa
     3721 agccacgtac tttttcaccg gtgaaaatcc gcaatgggaa cgggacaaac ccacaggaat
     3781 gaagctgtgt gattacctct atcgaaaggt ggagcggttc gccatcgaga acggtgtgca
     3841 ggtggttgat gccgggaagt gctcaccgga gcagattaac gcgatgattg aggtggtcag
     3901 cgagcggcac ggggaaagcg cggtgaacga gaagctgaaa gcggagctga tgcgggaata
     3961 taacagccgc tattttaacg tgccggtgcc tgaactgccg gaggacaaac ccgcccgccg
     4021 gtggtggccg ttcggcaaaa aataacgcca gcaccggcat ggccggtgct gttattcttc
     4081 cccgagctta tcccgcatca cctgccagag tgtttccgca tcctcgtgaa gccgttcgca
     4141 catgtcggcc ggggtatcca tgtccagctc gttcagcttt tccagcaaca gcagcgactg
     4201 atccttaatg aatttagcca tattgagggc agttttttcc tgcttcgtca tgttctccat
     4261 tcccttcggt aagttctcag ctaaaccagc cggaccagaa gcgttttttc ttctgtgtca
     4321 gttctgttgt caatctgtcc acttcggcct ggagtttccg gcgctcatca tcgtgctggc
     4381 ggcgttcttc ttcccgggat gttttatctt ccagcatcag ggtgacagcc tgccggagat
     4441 cggccagttc cttttgcatt gccagaagct gttcctgtgg gaaatttttt tgtgtatttt
     4501 cacggtgtga aatgccggga atttgtttct cagggatatt cacatttccg tatacacgga
     4561 tcagttctga cacatccacg acagggttgt ttttcccgtc acgtgacacg gtaacctttc
     4621 cctgttttac atgattgtat agcgttcttc tggtaatgcc cgcggcttgt gctgcctgtg
     4681 aaaggttgag caatgttttc gccactgtat acccctgatt ttatcgggtg tgaaaggtgt
     4741 gtaatttctc acggtaaatg tatcgctgaa ttttacccgt ttttccgttc aggatgtggt
     4801 tttctgacgc gctgcgcttg tcaaaccggc gacggccacg caatgaattg cgcccccgtc
     4861 atcggttatt agaggctgtc tgcccccgga tacccggatt ctttctgatg cctgacggtg
     4921 acgatcacaa tctggtccat ttcccggtcg tggcggtaga gcatcacata gccactgtcg
     4981 ccaaacccga tcaccagttc cttatattcc agcggcagaa acggcaccgg gcggccaatg
     5041 tctggcaatg ttttcagttg ctggatagcc cggacgatca cctcacctcc ttttctggct
     5101 gccagccgat ttttggtttt gagaaagtcc tgaaggcgtt gtaaatcgcg ctgtgccagc
     5161 gcggaaatcg ttacctgtgg catggcggag catcctgctc gttctcagtt ccccaggtac
     5221 ttatccaggc ttccgcttct tccgcagtaa ggtgcagacc cgtttcctga tagtgctgcc
     5281 atgcggcctg tccgtcccgc aggtactggc tgcgcttctc ttcccggtcg atgtattcgg
     5341 tgattgcctc cagcattagc gcgtgcgctg agcggtgccg gtcatcggcc agcgttttca
     5401 gccggtcctt aagttcttca tcgagcctga tggatgttgc tgatgacata aaagcccctt
     5461 tgtagtcata tgtattacac atgactactt taatccgtgg cggcgctatt tgccagaaat
     5521 acggcttttg ctgcccattt ttcagcttcg cttccactaa tcagggctgc tacaggctca
     5581 ctcatcatcc aggactattg cagcctgcgg gttaaaggac ctgttactgg cggcaaccat
     5641 catcattgag cttttgggtg gtgactggtc gcggatcctg taagccgtca tttttcagac
     5701 tgaattcaaa atttataacc tgtcgcattg ccgggtgccc tgcgggatat agccagtgcc
     5761 tcattgccgt cagcgtttcc cgaaaaaaaa ggtgacagcc ggtaagcgac acgttgtcca
     5821 ctcgtccatc gtccagaata tcaaacgtgg cggtcacctg accttcgatg cgttttgctg
     5881 cggcctgtac cgggtaggtt ggctgcagtg tggttacggg ttttagtggt atcgcatgta
     5941 ccggagtgag gaacatggcc gccagcactg tcagagtaaa agctttcatg gtctgaaatc
     6001 cagaggggcg tttttgaata tttgccaggt attttcatcc ggtgccggcg gaattttggc
     6061 gtgggtaacg gctgatattg ctgctttaca gagtttcgga tctcctcctt cagcaatggc
     6121 gctgttcaga gtgccatcac gttgtatgga tatccgcacg gtgcacattt ttcccgcata
     6181 ggcctctggt tcaaacatct gcatttcaac agcggtcttg atggccgcag cgtagctact
     6241 gattttagat gacttatcca gagtataaat tttcggcggc tgggtggtgg aatgcttaca
     6301 ggccgtcagg agtaaggggc tgcacaatgc cagcaggata aagggtatta aacgcatcgg
     6361 gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg tcactcttta cggccacgtt
     6421 catttacata aacacggctt atcgtatcgt ttttacacca aacggcccgc catgcagttg
     6481 cgtgcatagg catgcagcgg gttacaattt accgggcgcg tttccggcgg ttttccgggt
     6541 ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctcagaccgt ctgagcggtg
     6601 cgggcaatgc gacgttatgg taaatacccc ttaccggcgg cgctgtggct gccaggtgaa
     6661 ggtggtttcc gtagcctcgt atttctcccg acgcatttcc tgcgcccgcc tggcttcacg
     6721 ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt tgttttaccg cctttggcag
     6781 cgttttgttc cctttcaggc ccatggccag tgcctgagcg acaaaggcgt ccgccaggcg
     6841 cttttcttca gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc gtctcaggtt
     6901 tcccgaacag tttaaccgca ggtcctctcc tcatgccagt tagctttcag atgtgtctat
     6961 accatctgaa atgtaagtgt gatgaatgtc ttaaataata aaatttgttg ccatgagaaa
     7021 ttttcgggaa aagacgcgat ctgcttacag gtggctccgg gcgctttttt gtgcctgaag
     7081 atgagaacta atctcatata ttgacacctg aaaacgggag gagtaaggta ataattatac
     7141 tgtgtatatg tacagtgtgc gccgggagac cggttagaga tcaaggggtg aaagtccccg
     7201 accattgaag gaccagcaat ccacaaggtc cccgagtcat gcgttgcata ccgcgaggta
     7261 tggggcgaag cgttgacagg ggtgttgaca ggccagccat tgagccacga aatgtatatt
     7321 aaattaccgg gtgccgacgt tgtactgtta acggaaggca acatcatagg gtgcgacact
     7381 gcgagtgcca catggacccg gcggggtctg agaccctggc atgtcaatac gatctctacg
     7441 cgggaaccgg gagatctccc ctctgaccat ctgccagtgt cggagatggc ccgcaccggg
     7501 aagacgagga gtcatagccg gtgatgtacg gagaggagaa gtcggactcg ctcatagtag
     7561 cggcgaagca ggcgaacaac ccgaaaggag cggagtcagt ggagcgaagg agcggggcca
     7621 aggggaacgc ggaacagcca cacatgcgcc ggacacagag ccgggaaagc atgtcacaga
     7681 ggctgtcacg cgtgcgggaa gctgcgaagc agcggaagaa agaacggttt acagcattgt
     7741 tccacctgct gacagtcgaa gcactggaag ccgcattcct ctccctgagc aggaaagcgg
     7801 ccgccggagt ggatggcatc aggtggatgg actacgccgg aaacatgaag aacaacataa
     7861 cagatctgca ccggaggcta catcagggca gctacagggc gcagcccggc aggcgtcact
     7921 acatcccaaa agcggatgga aaacaacgcc cgctcggcat cgcctcgctg gaggacaaga
     7981 tcgtccagta tgcgctggtg aaaatcctga acgcagtcta tgaaaacgac tttatggggt
     8041 tctcatacgg gttcagaccc gggcgaagcc agcacgatgc actggacgca ctggccacag
     8101 ggctggtacg cactaacgta aactgggtac tggatgccga catcagtcag ttcttcgaca
     8161 gggtgagcca cgaatggctg atcaggttca cagagcatcg gatcggcgac cggagggtaa
     8221 tcaggctcat acgtaagtgg ctcacagccg ggacgtcgga ggagggtcaa tggcgagcaa
     8281 cggaggaagg caccccacag ggtgcggtca tctcaccgct gctggcaaac atatacctcc
     8341 actacgtctt cgatctgtgg gcgcatcagt ggcgacgtcg ctatgccaca ggcaatgtgg
     8401 taatggtcag atacgccgat gacatcgtca tcgggttcga caaacgatac gatgcccggc
     8461 gcttccgtat agccatgcag cgcagactga gggagttcgg actcacggtt cacccggaga
     8521 aaacccgtct gatggagttc ggccgcttcg ctgccgaaaa ccgtgccatc aggggaaaag
     8581 gcaaaccaga aacgttcaac ttcctcgggt tcacgcacat cagcgggaaa gatcgcaacg
     8641 gcaggttcat gctgatacga aagacccgcc gggatcggat gacggcaact ctgaaagcca
     8701 tcaaagacgg tctgcgaagg cgctggcatt actcaatccc cgaacaggga aaatggctca
     8761 ggagagtggt tcagggatac ctgaactatc actcggtacc gggcaacttc cccaccatgc
     8821 agaagttcag gacacacgta acaaacctct ggcgccgggc gctcaggcgc aggagccaga
     8881 aggatgatac gacctggacg aaagcaaaca aactggcagc cgcatggcta ccaagggttc
     8941 gggttcttca tccatggcct gtggagcggt tcaccgccag acacccgagg caggagcccg
     9001 gtgcgtaaat cgcgcacgcc gggatctgtg cggggggtat ccggtaacgg gtatccctac
     9061 cgcgacatgt tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg gtatgagtgg
     9121 tggagacggt cgaggtccgg gtaattcagg tctgggacat aatggtggtc aggccagtgg
     9181 gaatgtgaac ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta cggatccaaa
     9241 cagcgggccg ggctggggta cgacgcatac gccgaatgga gatattcata actataatcc
     9301 gggggagttt ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca gcggcaatca
     9361 tagcggtagc tctggtggcg gacagtcttc ggccactgcg atggccttcg gtctgcctgc
     9421 cctggctact ccgggggctg aaggactggc tttatcagtt tccggcgatg cgttgtcggc
     9481 cgccgttgct gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc tgtgggggat
     9541 tgcgatctac ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa tgatgtcaaa
     9601 aattatgacg tcattaccgg ccgatgcggt gacggagact ccggcaagta ctttaccact
     9661 ggaccaggcg acggttcgtg tcagacaacg ggttgtggat gtggtgaagg atgagcggca
     9721 gcatattgcg gttgtcgcag gtcggccaat gagtgtccct gtggtggatg cgaaaccgac
     9781 aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc aggtgagcgt
     9841 accgaaaggt gttccgacag cgaaagcccc gccaaaaggc attgttgctg aaaaaggtga
     9901 ttcacgtccg gctggtttta cagccggtgg taactcccgt gaggccgtta ttcgtttccc
     9961 gaaagagacc ggacagaagc cggtttatgt gtcggtgaca gatgttctta ccccggcaca
    10021 ggtaaaacag cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg ccgctcatcc
    10081 ggaagagggg ctgaaaagag actatgataa agcgaaagct gagctggatg ccgaagataa
    10141 aaatattgcg accttaaaca gccgcattgc atcgacagag aaggcgctcc ccggtgcaag
    10201 ggctgctgtt caggaagccg ataaaaaggt aaaagaggca gaggcaaata aagatgattt
    10261 tgtgacttat aacccgcctc atgaatatgg ctccgggtgg caggatcagg ttcgctatct
    10321 tgataaggat attcagaatc agaatgagaa attaaaagcg gctcaggcat ctttaaacgc
    10381 aatgaatgaa tccttatcca gagataaggc tgcacttacc ggggcgatgg agagccggaa
    10441 acaaaaggag aaaaaagcga aggatgcaga aaataagtta aatgaggaaa agaacaagcc
    10501 tcgcaagggg actaaagact acggccatga ttatcatcca gtacctaaaa cagaagacat
    10561 aaagggactt ggagagttaa agcgaggtga tcctaaaaca cctaaacaag gtggtggtgg
    10621 taagcgggcc cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg attcccagca
    10681 cggtgagctt gaagggtatc gtgccagtga tggcgaacac ctaggcgcat ttgatcccaa
    10741 aacgggtaag caggttaaag ggccagatcc aaaacggaac attaaaaaat atctttgaga
    10801 ggtaaatatg gggcttaaat tacgtttgac atggtttgat aagaaaactg aagagtttaa
    10861 aggggaagag tattctaaag actttggtga cgatggttct gtcattgaaa gcttgggaat
    10921 gcctttaaag gataatatta ataatggtga gtttgatttg aagaaagaat gggttccttt
    10981 actacagcca ttttttaaaa atcaaataaa acctgatgtg gataattgtt atatagcttt
    11041 tgattatcgg gacggtaact ggtaacaaaa aattatcaag gatgatttga aatactgact
    11101 tccggtgttc agggatgaat gccggagtct ttaaatacag ggaaagtata tgaaaaaggt
    11161 aaagactatt tttcttttta tattgattgt tgctggcttt cttctggccg catgtcaggc
    11221 aaattatatc cgggatgttc agggcggaac ggtggcacca tcgtcctcct ctgaactgac
    11281 ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    11341 gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa
    11401 gtcagttggg gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc
    11461 tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta
    11521 aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct
    11581 ttcccgggtt ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg
    11641 ggagttcttg cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt
    11701 gtgtgagcta tgagaaagcg ccacacttcc cgcaagggag aacggcggac aggtatccgg
    11761 taaacggcag ggtcggaaca ggagagcgca agagggagcg acctaccgga aacggtgggg
    11821 atctttaagt cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct
    11881 cccacagatg caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta
    11941 tggaaaaacg ccaccggcgc ggccctgccg
//
