LOCUS       NZ_CP066148             1919 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain ZLWT plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP066148
VERSION     NZ_CP066148.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16072565
            Assembly: GCF_016132505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Wang,Z. and Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Feed Research Institute, Chinese Academy of
            Agricultural Sciences, No. 12 Zhongguancun Nandajie St., Haidian
            Dist., Beijing, Beijing 100081, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.3; GapFiller v. 1.11; PrlnSeS-G v.
            1.0.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 16:30:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 4,939
            Genes (coding)                    :: 4,721
            CDSs (with protein)               :: 4,721
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 95 of 218
            Pseudo Genes (incomplete)         :: 132 of 218
            Pseudo Genes (internal stop)      :: 52 of 218
            Pseudo Genes (multiple problems)  :: 53 of 218
            Pseudo Genes (short protein)      :: 4 of 218
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066148.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZLWT"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2020-08-25T12:30:01Z"
     gene            123..359
                     /locus_tag="ICI43_RS25000"
                     /old_locus_tag="ICI43_25005"
     CDS             123..359
                     /locus_tag="ICI43_RS25000"
                     /old_locus_tag="ICI43_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            585..767
                     /locus_tag="ICI43_RS25005"
                     /old_locus_tag="ICI43_25010"
     CDS             585..767
                     /locus_tag="ICI43_RS25005"
                     /old_locus_tag="ICI43_25010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          1705..1878
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(1217..1738)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.308"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acgtcaccgg attaaaatcc gctcccgttc accgtttctg gctgtctttc agcaccgcca
       61 gcgcttcatc ggttctgctg tgtctggcag actgtgcgga gttattctga cgggagaatg
      121 ctatgaacag actgacaaac ctgacacctg cagagaaaaa atttcttgat gatgctattg
      181 ccgcagctga gagagcgtca ggcaaaaagc taaatcagcc gaaccggcat atcgtcctga
      241 acagagcgcg tgcgcagatt gagtcgcagc gttacgccga ccgccagcgt gcattacgtg
      301 aggatgagcg gcagcagtcc gagtttgcct ggtcacgtcc gcgcgcgccg cgccgctgat
      361 tgcgcctttt ggcggtttag tggtgctgac actccgaaat cgtctgccat tcacccgcgt
      421 gcatagccat gcaggtacgc agaaatcatc aggggcgttt tttctcgata tgggcacaga
      481 aaggtgtatg tttcaggccg gaggcctcag aaacgcgctg agggcgtttg aggcggtgcg
      541 gccaacggat gttatgttta atgttgataa caggagtaag agtgatgctt acatttattg
      601 gtgtagtggt agtcgccgtt attgcattta aattatttat gctttttttt agttcatccg
      661 aatctgccgt cgaacgtgct acccgtaaat atatggagaa ccctacgagt gctaactata
      721 aaataatggt ggctacacgc atgagaaatg agaagaaaag agggtgatat atgtttgagt
      781 ttattggttt tttatttttg cttttggtgt gttatgtctt tatacaaatg gttgctgtca
      841 gggtgtttcc tgaatatgga gtacggaaag cggaggaacg ataccgtcga aatccatcag
      901 cagaaaatga ggatctgtta tggagagcta gaagtcgtat aactaaacgc aagtaatgtt
      961 tcctaagtgt gttgataggc aaacatggtg ggggttacta ctctcggagt taatgatctt
     1021 cttgtgatcc tgtcgtttca gtcgtaatct cttgctctga aaacgaaaaa accacctggg
     1081 gaggtggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccaggt aactgggtat
     1141 acgagaccgc agataccgaa actgttcttt cagtgtagcc gcagtcaggc ccatacttca
     1201 agacctctga atctctctct cgtatatccc agttaccagt ggctgctgcc agtggcgctt
     1261 tgtcgtgctt tctcgggttg gactcaagat gatagttacc gggtaaggcg cagcagtcgg
     1321 actgaacggg gggtttgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     1381 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1441 aaggcaggaa caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat
     1501 agtcctgtcg ggtttcgcca ccactgattt gagcgtcagg tttcgtgatg cttgtcaggg
     1561 gggcggagcc tatggaaaaa cggcttcgcc gcggccctat cgcttccctg ttaagtctct
     1621 tcctggcatc ctccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc
     1681 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat
     1741 tctgctgacg cgccggtgcc gccttttttc tcctgccaca tgaagcactt cactgacacc
     1801 ctcatccgtg ccaacatagt aagccagtat acactccgct agcgctgcgt gactggttca
     1861 gggctgcgcc ccgaaacccg ctaaaaccac tgccgcgcct gcggcttgcc caacgtctc
//
