LOCUS       NZ_CP066147             3173 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain ZLWT plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP066147
VERSION     NZ_CP066147.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16072565
            Assembly: GCF_016132505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wang,Z. and Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Feed Research Institute, Chinese Academy of
            Agricultural Sciences, No. 12 Zhongguancun Nandajie St., Haidian
            Dist., Beijing, Beijing 100081, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.3; GapFiller v. 1.11; PrlnSeS-G v.
            1.0.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 16:30:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 4,939
            Genes (coding)                    :: 4,721
            CDSs (with protein)               :: 4,721
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 95 of 218
            Pseudo Genes (incomplete)         :: 132 of 218
            Pseudo Genes (internal stop)      :: 52 of 218
            Pseudo Genes (multiple problems)  :: 53 of 218
            Pseudo Genes (short protein)      :: 4 of 218
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066147.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZLWT"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2020-08-25T12:30:01Z"
     gene            complement(join(3133..3173,1..283))
                     /gene="mobA"
                     /locus_tag="ICI43_RS24975"
                     /old_locus_tag="ICI43_24980"
     CDS             complement(join(3133..3173,1..283))
                     /gene="mobA"
                     /locus_tag="ICI43_RS24975"
                     /old_locus_tag="ICI43_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            567..761
                     /locus_tag="ICI43_RS24980"
                     /old_locus_tag="ICI43_24985"
     CDS             567..761
                     /locus_tag="ICI43_RS24980"
                     /old_locus_tag="ICI43_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1596..2024)
                     /locus_tag="ICI43_RS24985"
                     /old_locus_tag="ICI43_24990"
     CDS             complement(1596..2024)
                     /locus_tag="ICI43_RS24985"
                     /old_locus_tag="ICI43_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(1988..2158)
                     /locus_tag="ICI43_RS24990"
                     /old_locus_tag="ICI43_24995"
     CDS             complement(1988..2158)
                     /locus_tag="ICI43_RS24990"
                     /old_locus_tag="ICI43_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            2316..2744
                     /locus_tag="ICI43_RS24995"
                     /old_locus_tag="ICI43_25000"
     CDS             2316..2744
                     /locus_tag="ICI43_RS24995"
                     /old_locus_tag="ICI43_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(293..529)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          797..1321
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.085"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(3..283)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="88.0"
                     /evalue="6.07e-54"
ORIGIN
        1 ccgtaatagc gatcagcacc tccgcatact ccctgtcccc gacacgcttg ccgtcgataa
       61 agagtttttt ctgtaacgcc cccagtctca taacttcttt cagtacccga tcatcagtca
      121 gtgagttaac tttcttaccg agagctgccg cccgtaaaaa accggaaacg gtcttgccgc
      181 attcagcagc cctttttctg atttcctggt cttcttctgc cgtcagacga acagggcgac
      241 tgatagcccg cctgcgcgta ttacttccac ttcgttttgt cataaaacat ggctccgtat
      301 ctgacatggg tgtcggggca aagccctgac cagggcaatt gtaatagcgt gcatgtatgc
      361 gcggtataac aattgcacat cctgtcctgt cagcaagctc gaattaccga caaaggcacg
      421 gcagtttttt ggcagtgaca gtgttgtctg acagaaatgc ccgtcaaaaa gccaagcggc
      481 agaatagcgg cagcaaaaca gtcgaaaagt ggtacgcgat ataatcacac aacgcagatg
      541 acagacagaa cggagaaaac gaaaagatga ataagcagca gcaagccgta ctcaacatgg
      601 cgggattcat aaaaagtcag agcctgacgc tgctcgaaaa actggacgca ctcaatgccg
      661 acgaacaggc cgccatgtgt gagaaactgc acgaactcgc agaagaactc cagaacagca
      721 tacagacacg ctttgaagcg gaaaatctta ccgggatata acgcatccgg acaggatgca
      781 gaacggcata gaggagcgta aggagaaaat gtcacataag gcgctcttcc gcttcctcgc
      841 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggtgtcagct cactcaaagg
      901 cggtaatacg gttatccaca gaatcggggg ataaaaccgg aaagaacatg tgagcaaaag
      961 acaaagaccc agaagaaggg cgcgcgggag gcgtttttcc ataggctccg cccccctgac
     1021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1201 tgttggtatc tcggttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1321 agacacgact taacgccact ggcaacggcc actggtaagc aggatgtgcg agagatgtaa
     1381 cgagagttct tgaagtggcg gcctaactga ggctacactg aaaggacagt atttggtgac
     1441 atgtctcgca caagactgtt accacggtta gagaatctgc caggattaac taaccttcga
     1501 aaaaccacct gccagggtgg ttttttcgtt tttacaatag tgagattacg acgagactga
     1561 atgaatctca agatttcatt tagcctgccg ctcgatcatt caaagttttg aggtagcttc
     1621 attgggtcat ggtatccaag ctctaaaagc tttatatggt tcggtgtcag ccgctggaaa
     1681 tgaataaccc agtcagaaac cttgtatttc ccatgaaacg tatctttaaa aaccggatcg
     1741 cctaaatgag catgccataa tttgttagct ctagcatact tctcaatagc ttctttgttt
     1801 tcgtaatgat caggcacttt ccacgatggc gatactttgc cagtccaagc tggatatcct
     1861 tttaaaggat cgccaaagag attgtatttt tcaaaatgct caatgaaatc atcgatttta
     1921 tcgagtgttt ttgcagaata tctggatgtg taataaaggt tgaaggtctc agagaatgag
     1981 acaatattca attgtggatt ttgcgcgcct gctccgctgc ccatgctctt ctctgctccc
     2041 gacttagacc agatggcatt gtaacagact tctcattcgc tctggaaatc aacagagaca
     2101 aggatacgcc aaaaacctca atatctttaa caagatcatg acgttgttcg gttttcatat
     2161 cgctaactcc gtatctaatt caggcaggaa aaaatctttt cctcgttttc agagtagtct
     2221 tttttggttg cacaataatg gcgccagtgt caccctattc atatgaatat accatataat
     2281 atatacttta aatattttgg ggcttaggaa aatcaatgac ttactccgtt aagaataaat
     2341 ggaaagctgg cggtttgtct cttggctgga cagcagtgcc taccgtattg ttttttatac
     2401 aaaacgagaa aaagctaact tcagtggcct ttaacacact acttaacctg atcgttcact
     2461 ggtggagttt gcaagagtgg ccgcatccgt caatggaaag tctagccata cgtatggggg
     2521 tttctgtacg gactgttcag agagctatca atgatcttga aaaagctaat ctcttggaca
     2581 agaaaccaac atcgaagagt gatcgccgat atggtggtcg caatatttat gatctaacaa
     2641 agctagttga ctacctagat acaatgggtc catcagtagc tgaacaagtt aagaaaccac
     2701 gccataaaaa accggtatat accgtaagaa aaacgacagc ttaaaaaagc acaatggaac
     2761 tgagcgcgag cgatgcacga tagcaaaacg atatattact tattgcattg gtcttggtag
     2821 ccctgcttct ggctaaacca ctgtcaggag taattggctt tgcctgtaat gggttcatcg
     2881 cttcacagtt aaagttatcg catagctcat atatttacca taatccctta attgtacgca
     2941 ccactgaaac gcgctgaacg cgattctgac gcagacaccg caaaaagaga tgttttaccc
     3001 cggaaaaaca ggaaggatgc gtcaggatcg ttttcagctc gctgcatagc tatgcatgaa
     3061 agcgaatggt gatcactttg ggagcttacg gtgttcatac cgtcagtttt cgacagtttt
     3121 ctctccggga agctaatctg ccataagcct ggataacagg gcacggtgat act
//
