LOCUS       NZ_CP066114             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-7 plasmid pEho7-6, complete
            sequence.
ACCESSION   NZ_CP066114
VERSION     NZ_CP066114.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747513
            Assembly: GCF_022023955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022023955.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:23:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,909
            CDSs (total)                      :: 4,795
            Genes (coding)                    :: 4,665
            CDSs (with protein)               :: 4,665
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 40 of 130
            Pseudo Genes (incomplete)         :: 87 of 130
            Pseudo Genes (internal stop)      :: 22 of 130
            Pseudo Genes (multiple problems)  :: 17 of 130
            Pseudo Genes (short protein)      :: 1 of 130
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-7"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEho7-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2014-07-02"
                     /collected_by="Public Health Ontario"
     gene            complement(163..390)
                     /locus_tag="GVI68_RS24480"
                     /old_locus_tag="GVI68_24375"
     CDS             complement(163..390)
                     /locus_tag="GVI68_RS24480"
                     /old_locus_tag="GVI68_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1598..1816
                     /locus_tag="GVI68_RS24485"
                     /old_locus_tag="GVI68_24380"
     CDS             1598..1816
                     /locus_tag="GVI68_RS24485"
                     /old_locus_tag="GVI68_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(2107..2292)
                     /locus_tag="GVI68_RS24490"
                     /old_locus_tag="GVI68_24385"
     CDS             complement(2107..2292)
                     /locus_tag="GVI68_RS24490"
                     /old_locus_tag="GVI68_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1973..2060
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(806..1334)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 cttgggggct tgattataaa ataccgcact tcctcttatg tcttgtgctt tttagacaac
       61 cctgaaagct tttagctttg gtgatgcaat ttgctgggat tttggcggag aaccacaggt
      121 aaagaaaaag gccacattag cggccttttt cggagaagat gctcagtgcg gaatttcgga
      181 gacttcttta acgtcggttt tattgatctg ctgtttgaca ccattcgcgt ctgtatagcc
      241 aagcaggcct gagtctgatt ctttaggttt gccgtcgctg acaatagtgc gaccgtcatt
      301 ggtgtgtatg gcatagcttg tgcgcgtgca gccggtgagg gctgaaagcg caactgccgc
      361 tgcgaacact gaaacaaaaa tctttttcaa agccagctcc ttttatccat atcagttttg
      421 gtcactaacc atctaactat aggtcatttt ttatgtgaaa agaggcttga tggtgggctg
      481 tatatcgagt taggcgcgag gtgctatggt caaagtgtgg tgtcaggcgg ggaggaggct
      541 tatatgagat gtaatccggc cgatacatat accaagatga tttaagtatt cctctttgtg
      601 aggttggatg tctaatttat aaaggatctt cttgagatcc tttttttccg cgcgtaatct
      661 cttgccctgt aaacgaaaaa accaccctgg caggtggttt ttcgaaggtt aggtaatcct
      721 ggcagatccc ctaaccgtgg taacagtctt gtgcgagaca tgtcaccaaa tttgtccttt
      781 cagtgtagcc tcactaaggc cgccacttca agaactcttg agacatctct cgcacatcct
      841 gtttgccaat ggccgttgcc aatggcgatt agtcgtgtct ttccgggttg gactcaagtt
      901 gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca
      961 tcctggagcg aactgccttc ccggaactga gtgtcaggcg tggaatgaga aaccgcggcc
     1021 ataacagcgg agtgacaccg gtaaaccgaa aggcaggaat gcgggggagc acgagggagc
     1081 caccaggggg aaacgcctgg tatctttaag ccgcatcggg tttcgccacc actgatttga
     1141 gcgtcagatt ctgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttggcgc
     1201 ggccttatgc tttcttcgtt aagtatcttc ctggcatccc ccaggaaatt tctgatccat
     1261 ccgtaagccc gtcccgctcg ccgcagccga acgaccgagc ggagcgagtc agtgagcgag
     1321 gaagcggaat atattctgta tcacattttc tcctgacgcg ttttctttca ctttctgcgc
     1381 ctgtcttatg tggcattaat gctatgtgtt actgccatgc tacatcttaa gccagtatac
     1441 actccgctag tgctccgtga ctggtccggc gctgcgcccg gaacccgcct gtaccggttc
     1501 agcagccgtt ccggcctgac tgcaattttt tttttttcat ccctgcccgc taccctgtaa
     1561 acctttcttc tgcgttgccg ttaacagtag gtaagccatg tttggttttt cttccggagc
     1621 cctgatgact cttagtgatg atgagcgaca cctgcttgtg tccgttgttt cggtctggct
     1681 acggcgtgcc ggaggggatg ccggagccat gatgctggat gcataccggc agattttgtc
     1741 tgaaaccgaa ccggccgtaa ggactgtcat gctcgaattt cttgaatccg ttcgcattca
     1801 ttacatttcg tcctgagcat tttctgtatt tctgtccata cttaaacgag gcggccgtca
     1861 cggacggtgg tcgtcgttcc atgatgctgt tatggacgag tgagagtaat ctaatctaac
     1921 ttgattgagt tccgggcgtg ccgcccggtt ttttttgctg tttcccggtg tccgttccgg
     1981 gacaggatgt gcatttgtaa taccgcgcac gcgccggcat cacaaatacc ctggtcaggg
     2041 ctgcgccccg acaccccgcc atcgcctcac ggccgcgcac tgaattgcgc acccgttcac
     2101 cgattttcac gcattttcct cgcctatctg tgcgttcagc atggcgtaaa gcgcttcggc
     2161 ctgatcgtgc aggtgctcac agcagccggc agcctcatcc agatccatct gctcgagccg
     2221 ctcaaggagg agaagtgtct gtgcgcggat aaatttggcc atattgaggg cggcggattc
     2281 ctgtctggtc actggtgatt ttccccgtct ggtgatgatt tttgcgtgaa aagagcatgg
     2341 cgtcattctg gtgcatagtc atgcatgccg ttaaaattta acaggcgcgt ttctgacggg
     2401 ttccggggtg gtttgttgtg gtttttgtca tggtttcgtc aggaacgcgc tgaacgcgtc
     2461 tcaggcggtg cgggcaacgg atgttatggt aaatt
//
