LOCUS       NZ_CP066113             4781 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-7 plasmid pEho7-5, complete
            sequence.
ACCESSION   NZ_CP066113
VERSION     NZ_CP066113.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747513
            Assembly: GCF_022023955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022023955.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:23:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,909
            CDSs (total)                      :: 4,795
            Genes (coding)                    :: 4,665
            CDSs (with protein)               :: 4,665
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 40 of 130
            Pseudo Genes (incomplete)         :: 87 of 130
            Pseudo Genes (internal stop)      :: 22 of 130
            Pseudo Genes (multiple problems)  :: 17 of 130
            Pseudo Genes (short protein)      :: 1 of 130
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066113.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-7"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEho7-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2014-07-02"
                     /collected_by="Public Health Ontario"
     gene            complement(155..511)
                     /locus_tag="GVI68_RS24445"
                     /old_locus_tag="GVI68_24340"
     CDS             complement(155..511)
                     /locus_tag="GVI68_RS24445"
                     /old_locus_tag="GVI68_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(714..1031)
                     /locus_tag="GVI68_RS24450"
                     /old_locus_tag="GVI68_24345"
     CDS             complement(714..1031)
                     /locus_tag="GVI68_RS24450"
                     /old_locus_tag="GVI68_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1218..1460
                     /locus_tag="GVI68_RS24455"
                     /old_locus_tag="GVI68_24350"
     CDS             1218..1460
                     /locus_tag="GVI68_RS24455"
                     /old_locus_tag="GVI68_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(2131..2346)
                     /locus_tag="GVI68_RS24460"
                     /old_locus_tag="GVI68_24355"
     CDS             complement(2131..2346)
                     /locus_tag="GVI68_RS24460"
                     /old_locus_tag="GVI68_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2353..2844)
                     /locus_tag="GVI68_RS24465"
                     /old_locus_tag="GVI68_24360"
     CDS             complement(2353..2844)
                     /locus_tag="GVI68_RS24465"
                     /old_locus_tag="GVI68_24360"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2879..3583)
                     /locus_tag="GVI68_RS24575"
                     /old_locus_tag="GVI68_24365"
                     /pseudo=""
     CDS             complement(<2879..3583)
                     /locus_tag="GVI68_RS24575"
                     /old_locus_tag="GVI68_24365"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3522..3845)
                     /locus_tag="GVI68_RS24475"
                     /old_locus_tag="GVI68_24370"
     CDS             complement(3522..3845)
                     /locus_tag="GVI68_RS24475"
                     /old_locus_tag="GVI68_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_126289757.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4494..>4616
                     /locus_tag="GVI68_RS24580"
                     /pseudo=""
     CDS             4494..>4616
                     /locus_tag="GVI68_RS24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(3959..4132)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          4099..4607
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.294"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(2040..3532)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3529..3845)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="7.01e-62"
ORIGIN
        1 tcgtttacag agcaagagat tacgcgtaca gagcacggat ctcaagaaga tcgtaagaag
       61 aaagttaaca tgagcgtttt tatccctcaa aaagaaggct tctagttata gaagccttct
      121 ttttgaggga taacttatat ttttttaaaa aaaatcatgc aatagcatgt ttcttctcag
      181 aactatcaac aatgtgtttc ctttctatga tttcatgagc gggaatcact ctgtctattt
      241 cttcttgcaa tcgagggcta ctagtaacct cccataaatc aacagactgc tgtatattta
      301 gccagaactt tgcagtggta tcgaaggctt tagccaacct aaaagccata tcggtagaca
      361 gctttctatt attgttaact aaagcactgg cagagtttct atgcacattc aacatttcag
      421 ccaagtcatt gatttttaaa ccacttggtt cgagatattc ataaagaata atatccccta
      481 cagttgatgg ttttctagtt gcttgggaca tgctttactc ctaaattttt tacttctctc
      541 tgttagatga ggaagataag tatttcgtct attaccagct gtagcgatga gtaattatct
      601 taaatatagt cacgtcattt tttttattaa tcattatttg taatgacatt ttaatgtagg
      661 tgagggaacg cctcacctac ttgttttata agataacagc cacgacctgt tatctatcgg
      721 taatcatgtg gatcgagata caaatcctcg actccgtttt caccccactt aaatatcaag
      781 cggtacttct cattcacccg tatggatgaa taccctgtca atggagggtt gagattctca
      841 aatcggtttc ccgggggaga ccgcaaatct ccaactttat cagcagcatt cagcatatcc
      901 agcttgcgac ctaatgaagc actaatcgca gttggaatct ttcgattggc tttaccatat
      961 ttgtaaaact ggtatagcca atcgtctcga aactgaatgc ggcaatcatc atgttcatga
     1021 tcttgctgca tttttctttt acctcaattt ttgccgcaac ttggcaattt tttcgatcac
     1081 cttcttacct ccgatatagt acagcaaaca tatcaaatgc acaacagcac agtgcacttg
     1141 tgcaatcaat ggctatgctg tagaatgcac tcatgcacag tgcgggtgtg caaaataaat
     1201 gataatttgg aggttgcatg gctcttgttc gctcaggaaa caaaagcaag atttttgaag
     1261 cagatacgaa gtttaactcg gtttttgaca ctgaatataa gccgggagaa acatgtgaat
     1321 atgaagggat ttataaatgt accggttgcg gtaaagaagt aactatcgca ggcgggaaaa
     1381 caatcccacc acaaaaccac caccaacata cccccgcgca agggaacatt cgttggcaat
     1441 tgtttgttta tgctcagtga ggtatagaaa atggcagatc gtcgaccact caacccacac
     1501 gttgtcgttc ctaaaccagg cggcggaact caacctcgtg accgtaacaa tgacggaagg
     1561 gtcagaggaa aacgctcaga tgcaggaaag ccacgaaaat aacagcaagc cgccagcctg
     1621 gcggcttttt tatatctgca gctgatactg atttaccata acatccgttg ggcgcaccgc
     1681 tgaaacgggg ttggctgcgt ttccggtgcc agacgcgaga aagtggcgac agacatgacg
     1741 aaaaaacacc agaaacgcag ccgctggata ttacactgat gcatagtcat gcagccagat
     1801 gcatggcgcg taaagtcaca ctaaaatcag cgcgaacgcc ttaaaaaccg acaaatgctt
     1861 ctccagccag tcccacagct ccgccgccag atacaggcag ttaagcaatg attcaataag
     1921 atttaacagc ttcatttccc gctcctgaat aagtcgtgac ctttttgatc acgaacctat
     1981 ttagcgggag aagtaagagc ccctcttcgt gcgggttgtc agctgcctgg atggtttaaa
     2041 gtgtcggccc gcggtaggtt ttctgcggct ggcgctccag ctccagtgtt ttctgatact
     2101 cattctccct ctgccggagc tcgatggcat tcagttcccg gacaagcggc gcagctgctc
     2161 gcttaaactc agcacctgcg tgctcaagtc cgacacgcgc tcgttcagca ccgcgttctc
     2221 ccgttgcata agcccacaca tgctccgcca ttccgcgaag gcgctctccc attcgtccag
     2281 cctttgcgag tagtcctgct gtagctgctc taatgcgctc agcaactgct tttccagctc
     2341 tgtcattttc cgctactcct gtttcaggcg gatccatgtc gtcccgtcgt aggggatggc
     2401 ttcggtttgc atccccggcg gcaggataag aaacctccgg ccgtcgctgc tctcctgata
     2461 gcgcactccc cacgtccggg cggtcatttt cgccagggta tcttcctgct gacgaagatg
     2521 cgtgtaattg tcggttatct gctgcccctg ccaccacagt atgctcccgc tcgtgccgat
     2581 cagcagcgct gacaccatca ggatggtgag ccatgtccgc ccggcagtcc gcagtacgct
     2641 ccgccggttt ttctccatcg cctcgctcat actctgctcg tgtgcgagga tggcgtcgct
     2701 gattctcctc gcgcttgcgc tcagttccgc tctgacagac tgctcgtgct cgctgaatgc
     2761 ggctttcagc atctcgccgg tactctgctt ctgcgctttc gattgctgct ctaagtcctt
     2821 cgccagcgtt aaaagactgc tcatagatgg ctcccttcag tcggatgttt cgccccccgt
     2881 ccgggtcggc aatgctgata ctgcttttcg tggtgcgcac cacctcaaaa cctgaccgtt
     2941 ccagcgcctc agtgacgtcc tgacgggttt tcagctcccc ggacgaagca agggtcagca
     3001 gtccgcgcgt gatggcttct gcggcctcct gcttcgtttt cggcagtccg gacggcgtga
     3061 ccagcgcccg gcggttttcc ggtgcgttcg ggtcgtgcag ccccagtctg ccgttcacga
     3121 tggtctgcca ggcatcgata cgcggccggt cggcgcggtc gtagtacggc tggagccgcc
     3181 tgccggtcag cagctcggta ttggggatca gaaagttcag ctccagacgc cccttgtcct
     3241 cgtgctccac ccacagaatg ctgtactgat ctttatcaag tccgggcatc agaacccgct
     3301 cgaagctggc catcagtttt tcccgctggc cgggaggcag atcggcctcc gcaaacgaca
     3361 ggacgccgga ggtgtatttc ttcacgtaag gcgacgcgtc aatcagctcc cggacttctt
     3421 ccggcttccc ctgcaggacg ctggcacctt cacgctgccg gtctttaccc agaagatagt
     3481 ccaccggccc ggcgccaccg ccgcgaccgc gtggatgaaa tttaacgatc atcgtccgtt
     3541 cctttctcaa gaacggcgtg ccgcagccgc tcaagcccgg catcgatggc catcagcgcc
     3601 gccacaatct gtaccctgtc atggccggtg ccgccgccgg cgttaacacg gcgggctatc
     3661 tggttcaggt tattgcccat gccggcaagc tggcgcagca gcgccggcga gattgagggg
     3721 agctttccgg tgcgcgcagg cttttcgtcc aggcaggtct gccgcatcca ggccgcgagc
     3781 tgtttaccgt cgcagcgttc cagcagacgg cggtgctcat cttcggtcac ccacatcgtg
     3841 agcattttgc tgcgtttctc tgacaaaccg gtctcctgat aacgccggga cgggcgggaa
     3901 cctctttccc ggtcggcgcg gggttggaca agccgcaggc gcgtcaggct tttggcgggt
     3961 ttcggggcgc agccctgaac cagtcacgaa gcgctagcgg agtgtatact ggcttattat
     4021 gttggcacgg atgcggaaat caatgaagtg cttcatgtga caggagaaaa aaggcggcac
     4081 cggcgcgtca gcagaatatg cgatacagga tatattccgc ttcctcgctc actgactcgc
     4141 tgcgctcggt cgttcggctg cggcgagcgg taccggctta ctggcggggc ggaaatttcc
     4201 tggaagatgc caggaagaaa cttaacaggg aagcgataag gccacggcag agccggtttt
     4261 ccataggctc cgcccccctg acgagcatca cagaatctga cgctcaaatc agtggtggcg
     4321 aaacccgaca ggactataaa gataccaggc gtttccccct gatggctccc tcgtgcgctc
     4381 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     4441 ttccacgcct gacactcggt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     4501 aaccccccgt tcagtccgac cgctgcgcct gttccggtaa ctatcgactt gagtccaacc
     4561 cggaaagaca cgacaaaacg ccactggcag cagccactgg taacaggatt agcagagcga
     4621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactggaa
     4681 ggacagtatt tggtatctgc gctctgctga agccagttac caggttaagc agttccccaa
     4741 ctgacttaac cttcgaaaaa ccgcctcccc aggcggtttt t
//
