LOCUS       NZ_CP066107             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-8 plasmid pEho8-6, complete
            sequence.
ACCESSION   NZ_CP066107
VERSION     NZ_CP066107.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747514
            Assembly: GCF_022023915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022023915.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:35:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,908
            CDSs (total)                      :: 4,794
            Genes (coding)                    :: 4,665
            CDSs (with protein)               :: 4,665
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 39 of 129
            Pseudo Genes (incomplete)         :: 88 of 129
            Pseudo Genes (internal stop)      :: 23 of 129
            Pseudo Genes (multiple problems)  :: 19 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066107.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-8"
                     /isolation_source="Dialysis fluid"
                     /db_xref="taxon:158836"
                     /plasmid="pEho8-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-06-02"
                     /collected_by="Public Health Ontario"
     gene            join(2327..2495,1..50)
                     /locus_tag="GVI69_RS24470"
                     /old_locus_tag="GVI69_24385"
     CDS             join(2327..2495,1..50)
                     /locus_tag="GVI69_RS24470"
                     /old_locus_tag="GVI69_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(341..526)
                     /locus_tag="GVI69_RS24475"
                     /old_locus_tag="GVI69_24390"
     CDS             complement(341..526)
                     /locus_tag="GVI69_RS24475"
                     /old_locus_tag="GVI69_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(892..1119)
                     /locus_tag="GVI69_RS24480"
                     /old_locus_tag="GVI69_24395"
     CDS             complement(892..1119)
                     /locus_tag="GVI69_RS24480"
                     /old_locus_tag="GVI69_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          207..294
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1535..2063)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 tcatgctcga atttcttgaa tccgttcgca ttcattacat ttcgtcctga gcattttctg
       61 tatttctgtc catacttaaa cgaggcggcc gtcacggacg gtggtcgtcg ttccatgatg
      121 ctgttatgga cgagtgagag taatctaatc taacttgatt gagttccggg cgtgccgccc
      181 ggtttttttt gctgtttccc ggtgtccgtt ccgggacagg atgtgcattt gtaataccgc
      241 gcacgcgccg gcatcacaaa taccctggtc agggctgcgc cccgacaccc cgccatcgcc
      301 tcacggccgc gcactgaatt gcgcacccgt tcaccgattt tcacgcattt tcctcgccta
      361 tctgtgcgtt cagcatggcg taaagcgctt cggcctgatc gtgcaggtgc tcacagcagc
      421 cggcagcctc atccagatcc atctgctcga gccgctcaag gaggagaagt gtctgtgcgc
      481 ggataaattt ggccatattg agggcggcgg attcctgtct ggtcactggt gattttcccc
      541 gtctggtgat gatttttgcg tgaaaagagc atggcgtcat tctggtgcat agtcatgcat
      601 gccgttaaaa tttaacaggc gcgtttctga cgggttccgg ggtggtttgt tgtggttttt
      661 gtcatggttt cgtcaggaac gcgctgaacg cgtctcaggc ggtgcgggca acggatgtta
      721 tggtaaattc ttgggggctt gattataaaa taccgcactt cctcttatgt cttgtgcttt
      781 ttagacaacc ctgaaagctt ttagctttgg tgatgcaatt tgctgggatt ttggcggaga
      841 accacaggta aagaaaaagg ccacattagc ggcctttttc ggagaagatg ctcagtgcgg
      901 aatttcggag acttctttaa cgtcggtttt attgatctgc tgtttgacac cattcgcgtc
      961 tgtatagcca agcaggcctg agtctgattc tttaggtttg ccgtcgctga caatagtgcg
     1021 accgtcattg gtgtgtatgg catagcttgt gcgcgtgcag ccggtgaggg ctgaaagcgc
     1081 aactgccgct gcgaacactg aaacaaaaat ctttttcaaa gccagctcct tttatccata
     1141 tcagttttgg tcactaacca tctaactata ggtcattttt tatgtgaaaa gaggcttgat
     1201 ggtgggctgt atatcgagtt aggcgcgagg tgctatggtc aaagtgtggt gtcaggcggg
     1261 gaggaggctt atatgagatg taatccggcc gatacatata ccaagatgat ttaagtattc
     1321 ctctttgtga ggttggatgt ctaatttata aaggatcttc ttgagatcct ttttttccgc
     1381 gcgtaatctc ttgccctgta aacgaaaaaa ccaccctggc aggtggtttt tcgaaggtta
     1441 ggtaatcctg gcagatcccc taaccgtggt aacagtcttg tgcgagacat gtcaccaaat
     1501 ttgtcctttc agtgtagcct cactaaggcc gccacttcaa gaactcttga gacatctctc
     1561 gcacatcctg tttgccaatg gccgttgcca atggcgatta gtcgtgtctt tccgggttgg
     1621 actcaagttg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca
     1681 tacagtccat cctggagcga actgccttcc cggaactgag tgtcaggcgt ggaatgagaa
     1741 accgcggcca taacagcgga gtgacaccgg taaaccgaaa ggcaggaatg cgggggagca
     1801 cgagggagcc accaggggga aacgcctggt atctttaagc cgcatcgggt ttcgccacca
     1861 ctgatttgag cgtcagattc tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     1921 ctttggcgcg gccttatgct ttcttcgtta agtatcttcc tggcatcccc caggaaattt
     1981 ctgatccatc cgtaagcccg tcccgctcgc cgcagccgaa cgaccgagcg gagcgagtca
     2041 gtgagcgagg aagcggaata tattctgtat cacattttct cctgacgcgt tttctttcac
     2101 tttctgcgcc tgtcttatgt ggcattaatg ctatgtgtta ctgccatgct acatcttaag
     2161 ccagtataca ctccgctagt gctccgtgac tggtccggcg ctgcgcccgg aacccgcctg
     2221 taccggttca gcagccgttc cggcctgact gcaatttttt ttttttcatc cctgcccgct
     2281 accctgtaaa cctttcttct gcgttgccgt taacagtagg taagccatgt ttggtttttc
     2341 ttccggagcc ctgatgactc ttagtgatga tgagcgacac ctgcttgtgt ccgttgtttc
     2401 ggtctggcta cggcgtgccg gaggggatgc cggagccatg atgctggatg cataccggca
     2461 gattttgtct gaaaccgaac cggccgtaag gactg
//
