LOCUS       NZ_CP066106             4781 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-8 plasmid pEho8-5, complete
            sequence.
ACCESSION   NZ_CP066106
VERSION     NZ_CP066106.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747514
            Assembly: GCF_022023915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022023915.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:35:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,908
            CDSs (total)                      :: 4,794
            Genes (coding)                    :: 4,665
            CDSs (with protein)               :: 4,665
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 39 of 129
            Pseudo Genes (incomplete)         :: 88 of 129
            Pseudo Genes (internal stop)      :: 23 of 129
            Pseudo Genes (multiple problems)  :: 19 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066106.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-8"
                     /isolation_source="Dialysis fluid"
                     /db_xref="taxon:158836"
                     /plasmid="pEho8-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-06-02"
                     /collected_by="Public Health Ontario"
     gene            complement(join(4699..4781,1..409))
                     /locus_tag="GVI69_RS24550"
                     /old_locus_tag="GVI69_24350"
     CDS             complement(join(4699..4781,1..409))
                     /locus_tag="GVI69_RS24550"
                     /old_locus_tag="GVI69_24350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<444..1148)
                     /locus_tag="GVI69_RS24555"
                     /old_locus_tag="GVI69_24355"
                     /pseudo=""
     CDS             complement(<444..1148)
                     /locus_tag="GVI69_RS24555"
                     /old_locus_tag="GVI69_24355"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1087..1410)
                     /locus_tag="GVI69_RS24445"
                     /old_locus_tag="GVI69_24360"
     CDS             complement(1087..1410)
                     /locus_tag="GVI69_RS24445"
                     /old_locus_tag="GVI69_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_126289757.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2059..>2181
                     /locus_tag="GVI69_RS24560"
                     /pseudo=""
     CDS             2059..>2181
                     /locus_tag="GVI69_RS24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2501..2857)
                     /locus_tag="GVI69_RS24450"
                     /old_locus_tag="GVI69_24365"
     CDS             complement(2501..2857)
                     /locus_tag="GVI69_RS24450"
                     /old_locus_tag="GVI69_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(3060..3377)
                     /locus_tag="GVI69_RS24455"
                     /old_locus_tag="GVI69_24370"
     CDS             complement(3060..3377)
                     /locus_tag="GVI69_RS24455"
                     /old_locus_tag="GVI69_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3564..3806
                     /locus_tag="GVI69_RS24460"
                     /old_locus_tag="GVI69_24375"
     CDS             3564..3806
                     /locus_tag="GVI69_RS24460"
                     /old_locus_tag="GVI69_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(4477..4692)
                     /locus_tag="GVI69_RS24465"
                     /old_locus_tag="GVI69_24380"
     CDS             complement(4477..4692)
                     /locus_tag="GVI69_RS24465"
                     /old_locus_tag="GVI69_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1524..1697)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          1664..2172
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.294"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(4..1097)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.616"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(1094..1410)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="7.01e-62"
     Typing          complement(4386..4780)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.485"
                     /coverage="99.0"
                     /evalue="1.82e-73"
ORIGIN
        1 tccggccgtc gctgctctcc tgatagcgca ctccccacgt ccgggcggtc attttcgcca
       61 gggtatcttc ctgctgacga agatgcgtgt aattgtcggt tatctgctgc ccctgccacc
      121 acagtatgct cccgctcgtg ccgatcagca gcgctgacac catcaggatg gtgagccatg
      181 tccgcccggc agtccgcagt acgctccgcc ggtttttctc catcgcctcg ctcatactct
      241 gctcgtgtgc gaggatggcg tcgctgattc tcctcgcgct tgcgctcagt tccgctctga
      301 cagactgctc gtgctcgctg aatgcggctt tcagcatctc gccggtactc tgcttctgcg
      361 ctttcgattg ctgctctaag tccttcgcca gcgttaaaag actgctcata gatggctccc
      421 ttcagtcgga tgtttcgccc cccgtccggg tcggcaatgc tgatactgct tttcgtggtg
      481 cgcaccacct caaaacctga ccgttccagc gcctcagtga cgtcctgacg ggttttcagc
      541 tccccggacg aagcaagggt cagcagtccg cgcgtgatgg cttctgcggc ctcctgcttc
      601 gttttcggca gtccggacgg cgtgaccagc gcccggcggt tttccggtgc gttcgggtcg
      661 tgcagcccca gtctgccgtt cacgatggtc tgccaggcat cgatacgcgg ccggtcggcg
      721 cggtcgtagt acggctggag ccgcctgccg gtcagcagct cggtattggg gatcagaaag
      781 ttcagctcca gacgcccctt gtcctcgtgc tccacccaca gaatgctgta ctgatcttta
      841 tcaagtccgg gcatcagaac ccgctcgaag ctggccatca gtttttcccg ctggccggga
      901 ggcagatcgg cctccgcaaa cgacaggacg ccggaggtgt atttcttcac gtaaggcgac
      961 gcgtcaatca gctcccggac ttcttccggc ttcccctgca ggacgctggc accttcacgc
     1021 tgccggtctt tacccagaag atagtccacc ggcccggcgc caccgccgcg accgcgtgga
     1081 tgaaatttaa cgatcatcgt ccgttccttt ctcaagaacg gcgtgccgca gccgctcaag
     1141 cccggcatcg atggccatca gcgccgccac aatctgtacc ctgtcatggc cggtgccgcc
     1201 gccggcgtta acacggcggg ctatctggtt caggttattg cccatgccgg caagctggcg
     1261 cagcagcgcc ggcgagattg aggggagctt tccggtgcgc gcaggctttt cgtccaggca
     1321 ggtctgccgc atccaggccg cgagctgttt accgtcgcag cgttccagca gacggcggtg
     1381 ctcatcttcg gtcacccaca tcgtgagcat tttgctgcgt ttctctgaca aaccggtctc
     1441 ctgataacgc cgggacgggc gggaacctct ttcccggtcg gcgcggggtt ggacaagccg
     1501 caggcgcgtc aggcttttgg cgggtttcgg ggcgcagccc tgaaccagtc acgaagcgct
     1561 agcggagtgt atactggctt attatgttgg cacggatgcg gaaatcaatg aagtgcttca
     1621 tgtgacagga gaaaaaaggc ggcaccggcg cgtcagcaga atatgcgata caggatatat
     1681 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtaccg
     1741 gcttactggc ggggcggaaa tttcctggaa gatgccagga agaaacttaa cagggaagcg
     1801 ataaggccac ggcagagccg gttttccata ggctccgccc ccctgacgag catcacagaa
     1861 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1921 cccctgatgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     1981 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcggttccgg gtaggcagtt
     2041 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgcctgttcc
     2101 ggtaactatc gacttgagtc caacccggaa agacacgaca aaacgccact ggcagcagcc
     2161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2221 tggcctaact acggctacac tggaaggaca gtatttggta tctgcgctct gctgaagcca
     2281 gttaccaggt taagcagttc cccaactgac ttaaccttcg aaaaaccgcc tccccaggcg
     2341 gttttttcgt ttacagagca agagattacg cgtacagagc acggatctca agaagatcgt
     2401 aagaagaaag ttaacatgag cgtttttatc cctcaaaaag aaggcttcta gttatagaag
     2461 ccttcttttt gagggataac ttatattttt ttaaaaaaaa tcatgcaata gcatgtttct
     2521 tctcagaact atcaacaatg tgtttccttt ctatgatttc atgagcggga atcactctgt
     2581 ctatttcttc ttgcaatcga gggctactag taacctccca taaatcaaca gactgctgta
     2641 tatttagcca gaactttgca gtggtatcga aggctttagc caacctaaaa gccatatcgg
     2701 tagacagctt tctattattg ttaactaaag cactggcaga gtttctatgc acattcaaca
     2761 tttcagccaa gtcattgatt tttaaaccac ttggttcgag atattcataa agaataatat
     2821 cccctacagt tgatggtttt ctagttgctt gggacatgct ttactcctaa attttttact
     2881 tctctctgtt agatgaggaa gataagtatt tcgtctatta ccagctgtag cgatgagtaa
     2941 ttatcttaaa tatagtcacg tcattttttt tattaatcat tatttgtaat gacattttaa
     3001 tgtaggtgag ggaacgcctc acctacttgt tttataagat aacagccacg acctgttatc
     3061 tatcggtaat catgtggatc gagatacaaa tcctcgactc cgttttcacc ccacttaaat
     3121 atcaagcggt acttctcatt cacccgtatg gatgaatacc ctgtcaatgg agggttgaga
     3181 ttctcaaatc ggtttcccgg gggagaccgc aaatctccaa ctttatcagc agcattcagc
     3241 atatccagct tgcgacctaa tgaagcacta atcgcagttg gaatctttcg attggcttta
     3301 ccatatttgt aaaactggta tagccaatcg tctcgaaact gaatgcggca atcatcatgt
     3361 tcatgatctt gctgcatttt tcttttacct caatttttgc cgcaacttgg caattttttc
     3421 gatcaccttc ttacctccga tatagtacag caaacatatc aaatgcacaa cagcacagtg
     3481 cacttgtgca atcaatggct atgctgtaga atgcactcat gcacagtgcg ggtgtgcaaa
     3541 ataaatgata atttggaggt tgcatggctc ttgttcgctc aggaaacaaa agcaagattt
     3601 ttgaagcaga tacgaagttt aactcggttt ttgacactga atataagccg ggagaaacat
     3661 gtgaatatga agggatttat aaatgtaccg gttgcggtaa agaagtaact atcgcaggcg
     3721 ggaaaacaat cccaccacaa aaccaccacc aacatacccc cgcgcaaggg aacattcgtt
     3781 ggcaattgtt tgtttatgct cagtgaggta tagaaaatgg cagatcgtcg accactcaac
     3841 ccacacgttg tcgttcctaa accaggcggc ggaactcaac ctcgtgaccg taacaatgac
     3901 ggaagggtca gaggaaaacg ctcagatgca ggaaagccac gaaaataaca gcaagccgcc
     3961 agcctggcgg cttttttata tctgcagctg atactgattt accataacat ccgttgggcg
     4021 caccgctgaa acggggttgg ctgcgtttcc ggtgccagac gcgagaaagt ggcgacagac
     4081 atgacgaaaa aacaccagaa acgcagccgc tggatattac actgatgcat agtcatgcag
     4141 ccagatgcat ggcgcgtaaa gtcacactaa aatcagcgcg aacgccttaa aaaccgacaa
     4201 atgcttctcc agccagtccc acagctccgc cgccagatac aggcagttaa gcaatgattc
     4261 aataagattt aacagcttca tttcccgctc ctgaataagt cgtgaccttt ttgatcacga
     4321 acctatttag cgggagaagt aagagcccct cttcgtgcgg gttgtcagct gcctggatgg
     4381 tttaaagtgt cggcccgcgg taggttttct gcggctggcg ctccagctcc agtgttttct
     4441 gatactcatt ctccctctgc cggagctcga tggcattcag ttcccggaca agcggcgcag
     4501 ctgctcgctt aaactcagca cctgcgtgct caagtccgac acgcgctcgt tcagcaccgc
     4561 gttctcccgt tgcataagcc cacacatgct ccgccattcc gcgaaggcgc tctcccattc
     4621 gtccagcctt tgcgagtagt cctgctgtag ctgctctaat gcgctcagca actgcttttc
     4681 cagctctgtc attttccgct actcctgttt caggcggatc catgtcgtcc cgtcgtaggg
     4741 gatggcttcg gtttgcatcc ccggcggcag gataagaaac c
//
