LOCUS       NZ_CP066100             2746 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-E1 plasmid pEhoE1-6, complete
            sequence.
ACCESSION   NZ_CP066100
VERSION     NZ_CP066100.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747518
            Assembly: GCF_022023895.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022023895.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:19:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,541
            CDSs (total)                      :: 5,425
            Genes (coding)                    :: 5,289
            CDSs (with protein)               :: 5,289
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 45 of 136
            Pseudo Genes (incomplete)         :: 93 of 136
            Pseudo Genes (internal stop)      :: 16 of 136
            Pseudo Genes (multiple problems)  :: 17 of 136
            Pseudo Genes (short protein)      :: 1 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066100.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-E1"
                     /isolation_source="surface water"
                     /db_xref="taxon:158836"
                     /plasmid="pEhoE1-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-08-04"
                     /collected_by="Public Health Ontario"
     gene            complement(17..391)
                     /locus_tag="I9464_RS27600"
                     /old_locus_tag="I9464_27565"
     CDS             complement(17..391)
                     /locus_tag="I9464_RS27600"
                     /old_locus_tag="I9464_27565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            720..908
                     /locus_tag="I9464_RS27605"
                     /old_locus_tag="I9464_27570"
     CDS             720..908
                     /locus_tag="I9464_RS27605"
                     /old_locus_tag="I9464_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(956..1183)
                     /locus_tag="I9464_RS27610"
                     /old_locus_tag="I9464_27575"
     CDS             complement(956..1183)
                     /locus_tag="I9464_RS27610"
                     /old_locus_tag="I9464_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225622915.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRACMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            <2473..2574
                     /locus_tag="I9464_RS27615"
                     /old_locus_tag="I9464_27580"
                     /pseudo=""
     CDS             <2473..2574
                     /locus_tag="I9464_RS27615"
                     /old_locus_tag="I9464_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     Typing          1657..2152
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.97"
                     /coverage="82.0"
                     /evalue="0.0"
ORIGIN
        1 gaactggtag acacctttat ccctgaagat gtttgaagag aaaacggcct atttcatagg
       61 tcgtaaagtc agggtttagc tctttatcat ccatccctac agcgctccct gcctcgatca
      121 ataccttctt catgttgcta actttaagcc cgtctttggt aaccaagatc tttccttttg
      181 gaagcttatg aatcacgtaa tccttgaagg aatgtgtgtc cttatctcca ccaggcaaag
      241 aaaagccctg acctaataac gattttaatg aattattctc ttcaattaag ttcaggacaa
      301 catcatcaac cgtcatgcca aagtcattca cgtactcttt taggctctca atagttgatt
      361 tctttagtct tacgtcagac ataccattca tattattatt accccaccgc tctaatttga
      421 agaaataata caacaaaata caaattatac aaataaatat tgttctgaat acaacaagcg
      481 gtctgtaggt gcgatgataa ctatgccgca ccgccagatt gaacataaca tccgttgcgt
      541 gcaccgcctg agaggctctc agggcgtttc tacgacctca cctgcaaaag aacacatctt
      601 accccctgaa aaccgctcag aaacgcgctg atgatttctg cgtacctgca tggctatgca
      661 ctcaagtgaa tgaagtaaga tttcagtctg cgatcaccat aaaaccggga aaaagccgta
      721 tgacaaaaca ggaatcagcc gcactgaaca tggcaaaatt tatccgggcg cagtcgctgc
      781 tgctgctcga aaaactcgac gtgctcgatc ttgatgacga ggcagccgac tgtgagcgaa
      841 tgcacgaaca ggcagaagcg ctttatcaga agctgagcgc acgcttcggt atacaggacg
      901 gcgaataaag agacgaaaag aacgctcact tagacgaaaa gcgtatccag ggcaactatg
      961 tatatccacc gtgtacatag tcatgcctga cgctttcgag caggtcgagc atgcaggcac
     1021 gacgagcttg attggtatcg gacacaatat ggcgatacgc ctcaaacata acagcacccg
     1081 catcaccgcc cgaacggcga agcatagcgg aaatcatccg cacaaagagc agacgttcat
     1141 cggggcaaag gttcataagt tctcctggtg aaaaaccaaa catgaaagca cccatctatt
     1201 aacgtcagct gtgaacgctt cattagaaga aaataggtaa acccgggaaa aagatccaaa
     1261 cgttaacatc acaggtaaac agaaacgtcg ggtcgatcgg gaaattcttt cccggacggc
     1321 gcggggttgg gcaagccgca ggcgcgtcag tgcttttagc gggtgtcggg gcgcagccct
     1381 gaaccagtca cgtagcgcta gcggagtgta tactggctaa acttcagaga tgccaaggaa
     1441 gggacaggat atgttttgta gcaccgccac cacggtggcg atactccaaa acgcctggtc
     1501 agggcacagc cccgacaccc cagagccgac tgtaggtcac tggacgaacg cggcggaatg
     1561 atgtcggatg acgtcatttt gcggcgtttg ccctatcctg catcgtatta aaaaatgcag
     1621 ctatggcgcg tacggtgcca aagataagcc cggagatatt ccgcttcctc gctcactgac
     1681 tcgctccgct cggtcgttcg gctgcggcga gcggtaccgg cttactggcg gggcggaaat
     1741 ttcctggcat cttccaggaa gaagcttaac agggaagcaa gaaggccacg gagaagccgt
     1801 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
     1861 ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct ccctcgtgcg
     1921 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc
     1981 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     2041 acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcgt cttgagtcca
     2101 acccggaaag acacgactta acgccattgg cagcagccat tggtaagagg attcacggag
     2161 cagggtagta gagtgcttct acagagttct tgaagtggcg gcctgactgc ggctacactg
     2221 gaaggacagt tttggtatct gcgctccgtg aaagccagtt accttcggaa gaaagagttg
     2281 gtagctcttg attccggcaa acgaaccacc gttggtagcg gtggtttttt tgtttacagg
     2341 acatgagatt acgacgagag taaaaggatc acaagaagat ccggctgttt accagtgatt
     2401 tggcttcagt attggtttcc gcctagcggg gccttatacg taaaaattcc acacctgact
     2461 tatcacacag gggagctccg caagtaccag agagcactga atagcaggcc acggaaatgt
     2521 cttggattcc ggcaaccttc agtggtcttt gctgagctaa gacaggcggc ttagcgttgc
     2581 acttcgaact tgaatccgcc cagaagcatt cagcagaagt cgctaacggt ttgcttgcaa
     2641 atgctatcaa taatgctttg cctaaagcag gaataaaaaa gccacctaca cggtggcttt
     2701 ttttctgtcc gggaactagc tgaattgaac ctaaaatccc ggccac
//
