LOCUS       NZ_CP066099             5112 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-E1 plasmid pEhoE1-5, complete
            sequence.
ACCESSION   NZ_CP066099
VERSION     NZ_CP066099.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747518
            Assembly: GCF_022023895.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022023895.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:19:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,541
            CDSs (total)                      :: 5,425
            Genes (coding)                    :: 5,289
            CDSs (with protein)               :: 5,289
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 45 of 136
            Pseudo Genes (incomplete)         :: 93 of 136
            Pseudo Genes (internal stop)      :: 16 of 136
            Pseudo Genes (multiple problems)  :: 17 of 136
            Pseudo Genes (short protein)      :: 1 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066099.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-E1"
                     /isolation_source="surface water"
                     /db_xref="taxon:158836"
                     /plasmid="pEhoE1-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-08-04"
                     /collected_by="Public Health Ontario"
     gene            complement(join(4642..5112,1..828))
                     /locus_tag="I9464_RS27570"
                     /old_locus_tag="I9464_27535"
     CDS             complement(join(4642..5112,1..828))
                     /locus_tag="I9464_RS27570"
                     /old_locus_tag="I9464_27535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            1523..1789
                     /locus_tag="I9464_RS27575"
                     /old_locus_tag="I9464_27540"
     CDS             1523..1789
                     /locus_tag="I9464_RS27575"
                     /old_locus_tag="I9464_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072050235.1"
                     /translation="MSVYCVSYDLNQAGQNYNALYDELKKSPGWCHPLDSTWLISTGET
                     AQQLSDRLRRHLDNNDTLLVIGVTKEYAGWLTQDTWDWMRTDI"
                     /name="hypothetical protein"
     gene            complement(2573..2995)
                     /locus_tag="I9464_RS27580"
                     /old_locus_tag="I9464_27545"
     CDS             complement(2573..2995)
                     /locus_tag="I9464_RS27580"
                     /old_locus_tag="I9464_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3232..3435)
                     /locus_tag="I9464_RS27585"
                     /old_locus_tag="I9464_27550"
     CDS             complement(3232..3435)
                     /locus_tag="I9464_RS27585"
                     /old_locus_tag="I9464_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_197377787.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELGVDRENGK"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3851..3964
                     /locus_tag="I9464_RS27590"
                     /old_locus_tag="I9464_27555"
     CDS             3851..3964
                     /locus_tag="I9464_RS27590"
                     /old_locus_tag="I9464_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(4201..4569)
                     /locus_tag="I9464_RS27595"
                     /old_locus_tag="I9464_27560"
     CDS             complement(4201..4569)
                     /locus_tag="I9464_RS27595"
                     /old_locus_tag="I9464_27560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     Typing          3557..3964
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 cttctggttt tttgcaaaat tttgtattga actaaataaa ttttttaaat tatcatcatc
       61 tgtttttata tttccaaatg gaaaatcagg tgcgacctca tcgaggtaaa tatcattaat
      121 ctggaatgct ggaagtattt tttttagcca agccttaaaa tcctcagact catttattaa
      181 cttcatggta attgctgtaa acatcccttc attagaacca gaaaggaata cgatattgtt
      241 aaaccaatta actactcgtt ttgaatgctc accattaaaa ccagcaacaa ccgaaacaaa
      301 tggaacatcc tcccttgttt tttgaataat cccttctttg ataaaaaggt ttgactcact
      361 aaaagccgcc ttatttacct caatagaaag accatctctt tcaaagagaa tggtttctct
      421 tgattttggt gtgtagtaca accattcttc ttttattttc ccctgaaaaa tagacaatcc
      481 atatctatat ttaaccccat ctgaaataaa agtcatttcc atttcagatg gtttatttgg
      541 tccatcctca tcaagcaaaa acggtattac gttctttgta atgctacttt caatagcttg
      601 cacggaatgc aaaacaatat ctttcattaa agaaattgtt ttcatgagat tagacttccc
      661 gctagcgtta gccccaaata tcagagcaga tttcagtatc cgaacatcac tcactgaaac
      721 cacgttagcc aagtttatgt cgtctttcgc ggatgacacc gccaacatgc tcatattttg
      781 agcatccata aaggaaaatg cgttagaaag actaaaatta accatcataa gaacacccct
      841 tacgccatcc ttaaacaaca aaatgagtaa atattgacaa aatcaggact taaggatagc
      901 atacaagcgt tctaaaacaa caggtgcgtt taatagatag cacgttatgg aacacttgtt
      961 aaaaaaagct aaatgcaggg ggtctatgag attttttcta taccaaatgg ttcctttagc
     1021 cgcagttgtc agctcagcag tgctagcttt acactcggtt tcaggatggg gatggtttct
     1081 ctttgtagca tttatgctca gcggagctga tacagtgaag aaacatctag gaaatgacgc
     1141 atgaaaggag aaaaacgacg ttttttgaag taagtgggtt agcctgtacc accagaacta
     1201 acccgtaaca aaccatacac aaatgcggca cttgagtacc acctcaagag cttgataaag
     1261 ccagccacaa gatttgttgg ttgctacagt acaattttga tagcaaaaat caacactcat
     1321 gtggcgaaaa ataggaaatg caccatgcag acagacgagt ctcaggcata catttttcgc
     1381 ccttatatca cagtaaaggg caaacggatc acccgtccaa acggtgggat gttcaaaatt
     1441 ccaattaatc gtgaaaagaa gaagtaagtt gtgactgcag ccctaacggg ctgccccaca
     1501 tttgattaaa gaggatggtt ctatgtcagt ttactgtgtc tcttatgacc tgaatcaagc
     1561 aggtcaaaac tacaatgcac tatatgatga gttaaaaaaa tcgccggggt ggtgccaccc
     1621 tttagattcc acatggctaa tatcaaccgg agaaacagcg caacaactgt cggatcgttt
     1681 gcgtaggcat ctggacaata atgacacctt gcttgtgatt ggtgtaacca aagaatacgc
     1741 aggatggctc acccaagaca catgggattg gatgagaaca gatatataga tataaagccc
     1801 tgcggggctt tttttacgtc tgtagctggt gtggatttaa cataaccccc taattgtacg
     1861 caccgctaaa acggcctcag ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc
     1921 acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg agtatgcacg
     1981 caagtgcata gcgcgaaaat tcacgccatt cagccagggt aagtcctgtt tccggcgttc
     2041 ttcccatccg aaaacggtga acggggacgc aatttattgc gtggccgtga agcgttgccg
     2101 gcgcagccgg aaaaccggtt ttcagcacgt tcagaaatcg ggttcgtcga cggccggcaa
     2161 tcacgttatg gcaagtcgcc ggacgactca ccacacgatt caccggcgaa tcttcactag
     2221 agtcgtaccc agagtcgccg agtctaaaaa acaaccaaac ggggagtcgt gtctagagtc
     2281 gggtcgttat taacttattg tttttatagt cctgagtcgt taccgactct atgacgactc
     2341 gccggggact ctatgacgac tcgtcataga gtcggatcta tataaataaa atttaaaaaa
     2401 cgaaatttca ggaaagtaac ccactgatat taaatacaaa tctgcaaaat gttaattttg
     2461 cgtggggtgt gggtattttt agtggtgagg agcgttcaga gatctgacga tttttgggaa
     2521 ggttcaggaa ctccccggga tggccggaga ggaaaacgga agcatgacgg ggtcaggatt
     2581 ttttccagaa tttccaccac ggggaatggg gtggtgaaga aggttcgtgc ttgtgctcca
     2641 gaagctgcat agcctgacga agggaatcaa tatgctcatc tcgagcctta acagcccctt
     2701 taagaccatc gttttcagct tcaagtaaag cgattctatg ttgtaaatca tgtaaaccaa
     2761 cgctgttaac acctgtagat tctgcgtcac tttttacatc atattttaca gtatcgatga
     2821 caccatcaag agaaccaaac actctaataa gctctgaagt atcaataact ttaatagcct
     2881 taccgtgctt tacaacagag gtgtaactta acttcccact gttaatcatc ttgtaaattg
     2941 tggggcgact aaccttaaca agccgtgccg cttcactaat agacactctg gccatcagtt
     3001 accatcctgt aattggggtc gtctcagaat tcggaaaata aagcacgcta gcggttgatc
     3061 tgtcaggttg aagcctgaga ggccgagcgc agatcgtcag aaaaggcgaa aaacgatcct
     3121 aatctgacgc aacataggtg gggtgcctga cgcccggttg aggcgtactt caactggaca
     3181 ccattccaga aagaccaagc atggcatggc ctgccgctgt cttaccgtgc tttatttccc
     3241 gttttctcta tcgaccccta attcatccag tctcgaggac agtgtacgga aaagatgctc
     3301 cgcatcatcg tggagtttct cacaaaggtc ggcctcgaca tcgagagcaa gctcgttaag
     3361 cttctccagc aggagcagag actgattctg gatgaattta gccatgttca gcgctgtttt
     3421 ctgttgtttc gtcataagaa gcctcagagc gtcagtgctc agtgtaaaac cagtctcttt
     3481 tccgcttcct cgctcactca gtcgctacgc tccggtcgtt cggctgcggc gagcggcaag
     3541 accattctta taaaagctcg agaaaaccga gcgatcaagg taggaggaaa agaggaggcc
     3601 gcgcagcggc cgtttttcca taggctccgc ccccctgaca agcatcacag aaacggacgc
     3661 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat acctatcatt tctgataggc
     3721 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgccat tccgctgtta
     3781 tggccgcgtt tatctcattc cacgccagac actcagttcc gggtaggcag ttcgctccaa
     3841 gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta
     3901 tcgtcttgag tccaacccgg aaagacatgc aaaagcgcca ctggcagcag ccactggtaa
     3961 ctgatttaga ggagttagtc ttgaagttat gcgccggtta aggctaaact gaaagaacaa
     4021 gttttggtta ctgcgctcct ccaagccagt taccttggtt ctaagagcgc caactcttgg
     4081 aaccttcgaa aaaaccgccc gccaaggcgg ttttttcgtt tacagagcga gagattacga
     4141 cgagaacaca gtgatctcaa gaagatcttg cctcaacata cagtacaatc gtaactaagc
     4201 ctatttcctg ctcttccaaa accgatgatc cttatcaaaa aaaactatat aaaacacatt
     4261 atcctttaaa aaaccagcaa cccttctttt ctggcctcct atatttttta taacacccca
     4321 gttctcatca actgatattt catcagggca tttaaaatca ttaactgctg aatctggaaa
     4381 tcgagtgtag ttagtaaaaa taccatcaga gctgactttt gttatcccat tagctgtaag
     4441 ataaacaagc ttttcaatta aatctggcaa actgccgtca accctccaag aatcaataga
     4501 ctgaccaata tcatcgacgt tatgaaaata tctaaaacta acagaaataa atctatctgt
     4561 agttttcata atgctctttg aggattttgt ttcctctttc ctcgaagagc tacgaagacc
     4621 gcggcctctc tttgacataa atcatccctc aggcataagt ttttcaaatt catctaaatt
     4681 atcaaacaat gggatagcat caaatgcacc agataaataa tcatgcccat acgatgattt
     4741 taaagttcgc tgcttctctt tatactcaac caaagaatag agatgactac ttcctgattt
     4801 tatatcttta tcaacaaacc aaatctggtc acgtctaaaa caatcagtgt tcattagatt
     4861 aacatcctga acagcagcta ttatttggct aagcccctcg ctttgcgcat ggaatattct
     4921 aaacaaaaac ttagacaaaa gggtgtgaaa cttagaatca aactcatcaa ctaacaataa
     4981 gttaccgttt atcatggcat tgtagattgg accaagaaga taaactatct ttttagtacc
     5041 ctcagactca aaatcaatag gtactgaaat ctcaccgcta gctgtttttt tcactactct
     5101 aagagaaaaa ac
//
