LOCUS       NZ_CP066091             9294 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Citrobacter koseri strain FDAARGOS_1029 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP066091
VERSION     NZ_CP066091.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357198
            Assembly: GCF_016128275.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Kerrigan,L., Long,C., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Kerrigan,L., Long,C., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.11.1
            Genome Coverage                   :: 1371.54x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,426
            CDSs (total)                      :: 4,307
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 27 of 55
            Pseudo Genes (incomplete)         :: 26 of 55
            Pseudo Genes (internal stop)      :: 12 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            Pseudo Genes (short protein)      :: 1 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066091.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1029"
                     /culture_collection="ATCC:BAA-895D-5"
                     /db_xref="taxon:545"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA:VA"
                     /collected_by="ATCC"
     gene            join(9245..9294,1..328)
                     /locus_tag="I6H86_RS21950"
                     /old_locus_tag="I6H86_21950"
     CDS             join(9245..9294,1..328)
                     /locus_tag="I6H86_RS21950"
                     /old_locus_tag="I6H86_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(390..689)
                     /locus_tag="I6H86_RS21955"
                     /old_locus_tag="I6H86_21955"
     CDS             complement(390..689)
                     /locus_tag="I6H86_RS21955"
                     /old_locus_tag="I6H86_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(679..942)
                     /locus_tag="I6H86_RS21960"
                     /old_locus_tag="I6H86_21960"
     CDS             complement(679..942)
                     /locus_tag="I6H86_RS21960"
                     /old_locus_tag="I6H86_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(1248..1667)
                     /locus_tag="I6H86_RS21965"
                     /old_locus_tag="I6H86_21965"
     CDS             complement(1248..1667)
                     /locus_tag="I6H86_RS21965"
                     /old_locus_tag="I6H86_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2070..3755
                     /locus_tag="I6H86_RS21970"
                     /old_locus_tag="I6H86_21970"
     CDS             2070..3755
                     /locus_tag="I6H86_RS21970"
                     /old_locus_tag="I6H86_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3765..4022
                     /locus_tag="I6H86_RS21975"
                     /old_locus_tag="I6H86_21975"
     CDS             3765..4022
                     /locus_tag="I6H86_RS21975"
                     /old_locus_tag="I6H86_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            4107..4256
                     /locus_tag="I6H86_RS21980"
                     /old_locus_tag="I6H86_21980"
     CDS             4107..4256
                     /locus_tag="I6H86_RS21980"
                     /old_locus_tag="I6H86_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5737..7701
                     /locus_tag="I6H86_RS21985"
                     /old_locus_tag="I6H86_21985"
     CDS             5737..7701
                     /locus_tag="I6H86_RS21985"
                     /old_locus_tag="I6H86_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_012000836.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAACLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            7701..8432
                     /locus_tag="I6H86_RS21990"
                     /old_locus_tag="I6H86_21990"
     CDS             7701..8432
                     /locus_tag="I6H86_RS21990"
                     /old_locus_tag="I6H86_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            8439..8969
                     /locus_tag="I6H86_RS21995"
                     /old_locus_tag="I6H86_21995"
     CDS             8439..8969
                     /locus_tag="I6H86_RS21995"
                     /old_locus_tag="I6H86_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8997..9176)
                     /locus_tag="I6H86_RS22000"
                     /old_locus_tag="I6H86_22000"
     CDS             complement(8997..9176)
                     /locus_tag="I6H86_RS22000"
                     /old_locus_tag="I6H86_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..8755
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4360..4938)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7702..8429
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 aagttccctg tatcgggata tggcgaaagg ccgcgtatca taccgtacgg aagctgatgg
       61 cggccgggtg gtggatacca gcgagcttat ccgggtgtac ggtgagttaa gacaggatga
      121 gactcacgag cgggacgagt tgagactgtc gggtgagact gaaaaaatgg cctccgacgc
      181 gttgatcgcg gagataaaag cactcaggga ggaggtggcc gggctacggc aggaaatgca
      241 gtccatgaga ctgctggaac acaaaccgga gacagcagag accccggccg gaacaccgcg
      301 ccggtggtgg ccgtggggaa aacgctgacg cgctgtgctt gtccaaccgg cgacggccac
      361 gcaatgaatt gcgtccccgt caccggttat tagagaacaa tatggggatt ctggcgctgg
      421 ccgaccacgg ccagaacccg gacttttctg gtctggtcat caacatcata gaggacatgc
      481 tgtcccatca gactgatccg gcgaacgtcc tcgccatagc gtgagtccac ttcatagagt
      541 cgcgggaagt cggccagttt gtccgccgca gcgagaaccc ggtcggtaac gttctgcgca
      601 tcccacaggg atgaattttc cgcgatatag tcaaacagcg tcgttacatc ctcgatggct
      661 ccctgcttcc attccacgtc atacggcatc gcgcagttcc tgataacggc catgcaggcg
      721 ctggcggagt tgttccgtgg tcatacccgg acgcgtgtca gcacgggcgg cggtcacttt
      781 ggcgcgaacc tgatcttcat actctgcctg ggccagttct tcgttagcca gtcgctgcaa
      841 cagcgcagct ttgtccgggc caccttccgg cagtatgcgg ggcaggcttt cagccagttc
      901 ccgaagaaag ttggcgtttt cgtagtgtgt agtgttgctc attgcggagc ctcccgggtt
      961 aatgcggcaa tatgaaggaa gtttagcagg gggcggtctg aaagaatatg gtcgtgtcag
     1021 tcaggctgat gaagcagaaa gtcggtaaaa aagggcttac ggcatccatt ttacgtcaaa
     1081 acatatgcca tgcagttgcg tgcataggca tgcattagga taaaatttat cgggcgcgtt
     1141 tccggcagtt ttccgggggg gttgttgcct gttttatccc gtagccgccg gaaacgccct
     1201 gagcccgtct gagcggtgcg cgtaatgacg cgttatggta aatccactta ctgtctgcct
     1261 tcatagccat cgagataaac agctgaaatg gtgtcagcca gtagccttga ttttttcggt
     1321 gtgaggtctt tgctgcggta atcggtaccc gcaaattgtg ttgtgccttg gagtgagttt
     1381 aaaaattcct gactgtgaaa ctgctgaatg cgttgctgca catcactccg tgattttccg
     1441 gtcgctttat cctttgcgcc catatcccag aactgccgga aaaaaggcac catcattcgt
     1501 gttgtgtcag ctttctgggt ggaaaaattg ggatcaaaac cgtcatctga agcatatacg
     1561 taatgccggg tatgctgttc cacgctgccc tttggagcac aggcagtgag agttgctgtc
     1621 gctgttatca tggcgataag acacagtttg ctgccggaac gtctcatgag cgtctcctta
     1681 cctttaattc ttttttctca ttgagttata tatccgcagg ctatcttatc atcagtttct
     1741 gaccgtgttc agtgaccgaa caacccgaac cagacccccc gtttcttttc ctgtcccgga
     1801 gggggctttc tggtatctca tataatgatt tcagtaaaac atactgaggc cggagaacgg
     1861 cgccgtttgt gctggttttt gttgcatctg gaggggattg cgcatggtgt gagtggctgt
     1921 ttttttgatc attttcacaa aaacgcaaat ttacagactt gacatgaaaa acaaggcagc
     1981 gtaattttta tactgtatat aaaaccagtg gttatatgta cagtatttta ttttagttta
     2041 ttgttttaaa agtcaaagag gaaaagtata tgagtggcgg agacggacga ggtccgggta
     2101 attcaggtct gggacacaat ggtggtcagg ccagtgggaa tgtgaacggt acatcaggta
     2161 aaggtggccc ttcatcaggt ggtggtacgg atccaaacag cgggccgggc tggggtacga
     2221 cgcatacacc taacggggat attcataact ataacccggg ggagtttggt aacggcggga
     2281 gtaaacccgg tggtaatggc ggtaacagcg gcaatcatag cgggagctct ggtggcggac
     2341 agtcttcggc caccgcgatg gccttcggtc tgcctgccct ggccactccg ggggctgaag
     2401 gactggcttt atccgtttcc ggcgatgcgt tgtcggccgc tgttgctgat gtgctggctg
     2461 ccctgaaagg tccgtttaag tttggtctgt gggggattgc gatttacgga gtgctgcctt
     2521 ctgagatagc aaaagatgat ccgaaaatga tgtcaaaaat cgtgacgtca ttgccggcag
     2581 acacggtaac ggagacgccg gtcagctccc tgccgctgga ccaggcgacg gtcagcgtca
     2641 ctaaacgtgt ggcggatatt gtgaaggacg agcggcagca tattgcggtt gtcaccggcc
     2701 ggccaatgag tgttcctgtg gtggatgcga aaccgacaaa acgtccgggg gtattcagtg
     2761 tgtcgattcc gggtctcccg tctctgcagg tgagcgtacc taaaggtgtt ccggccgcga
     2821 aagccccgcc aaaaggcatt attgctgaaa aaggtgattc acgtccggct ggttttacgg
     2881 ccggtggtaa ctcccgtgag gccgttattc gtttcccgaa agagagcgga cagaaaccgg
     2941 tctatgtgtc ggtgacggat gttcttactc cggcacaggt aaaacagcgt ctggaggaag
     3001 aaaagcgtcg ccagcaggca tgggacgctg ctcacccgga agaggggctg aaaagagagt
     3061 atgataaagc gaaagctgag ctggatgctg aagataaaaa tattgcgacc ttaaacagtc
     3121 gcattgcatc gacagagaag gcgatccccg gtgcaagggc tgctgttcag gaagccgata
     3181 aaaaggtaaa agaggcagag gcaaataaag atgattttgt gacttataac ccgcctcatg
     3241 aatatggctc cgggtggcag gatcaggttc gctatcttga taaggatatt cagaatcaga
     3301 atgagaaatt aaaagctgcc cagacatctt taaacgaaat gaatgaatcc ttatccaggg
     3361 ataaggctgc gctttccggg gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg
     3421 atgcagaaaa taaattaaat gaggaaaaga aaaaacctcg taagggaact aaagattacg
     3481 gccatgatta ttttcctgat cccaagactg aagatattaa ggggttggga gagttgaaag
     3541 agggtaaacc taaaacccct aaacaaggtg gtggtggtaa gcgggcccga tggtatggtg
     3601 ataaaaagcg taaaatttat gaatgggatt cccagcacgg tgagcttgaa gggtaccgcg
     3661 ccagtgatgg cgaacacctc ggggcattcg atccaaaaac gggtaagcag gttaaagggc
     3721 cggatccaaa acgaaacatt aaaaaatatc tttaagaggt aaatatgggg cttaaattac
     3781 atattcattg gtttgataag aaaaccgaag agtttaaagg tggtgaatac tcaaaagact
     3841 tcggtgatga tggttctgtc attgaaagtc tggggatgcc tttaaaggat aatattaata
     3901 atggttggtt tgatgttgaa aaatcatggg tttcgatatt acagccacac tttaaaaatg
     3961 taatcgatat tagtaaattt gattactttg tatcctttgt ttaccgggat ggtaactggt
     4021 aagcaaaagt tatcaaggat gatttgaaat acagacttcc ggtgttcagg gatgaatacc
     4081 ggggttttta aatacaggga aggtatatga aaaaggtaaa ggttgttttt ctttttatat
     4141 tgattttttc aggctttctt ctggtcgcat gtcaggcaaa ctatatccgg gatgttcagg
     4201 gtggaacggt ggcaccatcg tcctcctctg aactgacggg gatcgcggtt cagtagaaaa
     4261 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga
     4321 aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacc
     4381 gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccggact
     4441 ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg cgaactccca
     4501 gttaccaatg gctgctgcca gtggcgtttt gcgtgctttt ccgggttgga ctcaagtgaa
     4561 cagttaccgg ataaggcgca gcagtcgggc tgaacggggg gttcttgctt acagcccagc
     4621 ttggagcgaa cgacctacac cgagccgaga taccagagtg tgagctatga gaaagcgcca
     4681 cacttcccgc aagggagaaa ggcggaacag gtatccggga aacggcaggg ttggaacagg
     4741 agagcgcaag agggagcgat tcatcggaaa cggtgatgat ctttaagtcc tgtcgggttt
     4801 cgccactcct gactgattca tggttgagcc accggctccc acagatgcac cgaaaaagcg
     4861 tctgtttatg tgaactcagt caggagggcg gagcctatgg aaaaacgcca cctgcgtggc
     4921 cttgccgttt tgcctcacat gttagtcccc tgcttattca cggaatctgt gggtaacttt
     4981 gcatgtgtcc gcagcgcccg ccgcagtctc acggccggag cgtagcgacc gagtgagcga
     5041 ggaggcgcaa tttccccggc acctacaaga cggacaccgg acgcgctgat gtggcatatt
     5101 gtcccctgat taagtcttta tattccctga taataaatta ccaggatagg tgaatcatat
     5161 cggtttttcg ctgaacattt tacgttaacc tatctctgtt gcttttttct ccttacggag
     5221 aaatacaggt catccccgcg agggcgtggc tgacctcagg gggatatccc ctgcttacgc
     5281 tggctggacg ccaccgacgc acggtctctc cccctctttc tggctccgtc cggctggacg
     5341 ccggccacgc cagaaattca cccggccccc gtcaggctca cgcctgacca cccacaaaaa
     5401 tccacggtgc tggacgcacc tggcgaagca gcaaagctgc tcctgatttt gcgggtggtc
     5461 agccgctcgc tatggcgaac tctgacgggg gccggaacac cggaaaagac cgcactgggg
     5521 tgccgccttt tcctctcaca cgctggggcg tgctcgttgt tcctccgggg ctgcgccttt
     5581 tccgcatggg gcggaaagag gctggcctcc gtggtgaaag ctgaacgcaa tcaccacgga
     5641 cgtgaccgcc gccgccatgc cttggggcat gtccgcggct tgtgcagcgg gggctgcaca
     5701 tcacgccggc aaaccatcgt ctgaaggaga gagaacatgt ttaatacgga ttcgcttgcc
     5761 tggaagtggc acattccgga gctggcccgg cagatttatc cgtgggtcag ctggctgtac
     5821 caggagtatt acggtctggc cggggctttc ctagttatcg gcctgttttt cggtctggct
     5881 gaactttggg ccggacggat gcgttttgtg gggctgagtc ataaggagcg acgggagtat
     5941 gccgatcgct ataagcgcga taataaacgg ccagcgccat tttcggtgct ggcctcgctg
     6001 gattcgcccg ggcggatggt cagggcacgt cgacatgtct ggaactacgc gtactggctg
     6061 accggcgcgg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc
     6121 tggggagaaa ccgtcctgcg tccggacctc tacgcaaacg cagattatta cctgctgcag
     6181 ccggctaacc tgttcgggat tgcctggtgc gcgggcgggc tggctgtcgg gattgtgttg
     6241 gcgcggctgc tggcgttcct gctgcttgac ggtcattttg cggctgctga cgaggcggtg
     6301 aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggacaggaga gatgaccgac
     6361 gtgcgccacc tgcatttcgg ggagccggta ccggttaatg cgctggccga tttttcctcg
     6421 gagcaggccc ggcaggagca ggcggtgttt ctgggtaagg atgagcaggg caagccggtg
     6481 ctggttccgc gtgatacctg gcgcaagacc aacattcaga tcctcggcct gcccgggagc
     6541 ggcaagagcg tgatggccac caacgcgctc attcgctgcg tacgcgattt cggggacgcg
     6601 gtggtgtact tcgaccccaa aggggacgca tgggcgccgc acgtcttccg ggcgcactgt
     6661 ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc
     6721 gatctcgacc agtacgcgct gaaaaacctg ctgattgccg ggcttaacct gggcgagacc
     6781 ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag
     6841 cagtttccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc gctgccggag
     6901 gccctgaaaa aggacgtgaa ggggttgatc actaggctgg aaaacgtcgc tgacctgagc
     6961 gtcctgcaga cggacagtgg catcgatgtg gcggggatta tcaacggcgg cggctgcctg
     7021 tacgttatcg gctcgatgga cgacgaggcg gtgatccgtg tgcaaaaaat gctgtttgcc
     7081 cgctgcgcgc aaatcatcat tgcgcgggac gagttccggg agtggccaca cgtcagcgtg
     7141 atgctcgatg aaattaaata tctgctgtcg aagtatgtac tgaacgcact ggggacgctg
     7201 cgcagccggg actgcaatct gttgctcgcg caccagtcgc tgggcgattt cgggcagtgc
     7261 gggcaggatc tgcccgctga atttgtgaaa accacggtac tggataacac gccgatccgc
     7321 tggttttacc gggcggcctg cctggagtcc gcgcagtggg cggcggggca gaccggggaa
     7381 atcagggtgg acgtggagcg ccgccgtgcc agccgggagg cggggaacgt ggagcatatc
     7441 agcggggaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcaa
     7501 cacctgccgg acgggttcgc ggtcatgacc gggctgggtg tggcccggat ggcgttcagc
     7561 agtccgctgc gggttgagcg ccgggaaatc ccgttgcagc gtttcccggt gctggcgaaa
     7621 accgatcccc tggcggagtt ccggcccccg gccgacggcg gcgggccgaa agcggatgat
     7681 gattttgcgg ggatttactg atggcgctgg agctgtacaa cgtcagccac gcgaaacgac
     7741 aggcgcggaa cgcggagaaa acccggctga ccctgcgctg gttgcgggaa gagctgtgtt
     7801 ccacggcgga gctggttgcg cggcggctgg ggattgcggc ggtgcagccg gtctaccgct
     7861 tcctcgacag cctggtggcg aaagggctgc tggtccggac caaatatccg gtggacggcc
     7921 gtcaggtcag cgtgtggggg ctgacgccgc atggcgtggc gttctcgttc gacgaggacg
     7981 aaccgctggc ggatgttatt ccgtttcagc cgtcaagggt gtcggcagca catctgccgc
     8041 accggctggc cgtgcaatcc ctgcgccttg atatggaagc cagaggggcc accggctggc
     8101 gcattctgca ccgtctgagt cttaaaggca tgaaagtgcc ggacgccctg gcggagctgg
     8161 acggtaaaac ggtggcgttt gaggtggagc gcaccgtcaa gagccgtcgc cggtatcagg
     8221 aagtggtgtc cgggtatctg ttcaaccgca aagctaacgg catcgatgaa atctggtaca
     8281 tctgcccgga ccgggcgacg caggtcaggg tgcagcgggc aatcctgtcg gtggatgaaa
     8341 tcgtgaaccc acagaccggc gaggcccgca aaacggcaga gctggatcgt gaacggttgt
     8401 ttgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac atggtattta
     8461 ttgaagacgc ggaggaccgg aacggcgagc gctgcatcat gccgaccagc gagcatctgg
     8521 aagatatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt gtcgaccggg
     8581 cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc aacagcgacg
     8641 aaaaagccac gtactttttc actggtaaaa atccgcaatg ggaacggaac aaacccacag
     8701 gaatgaagct gtgtgattac ctctatcgca aggtggagcg gttcgccatc gagaacggtg
     8761 tgcaggtggt tgatgccggg aagtgctcac cggagcagat taacgcgatg attgacgtgg
     8821 tgagtgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag ctgatgcggg
     8881 aatataacag ccgctatttt aacgtgccgg tacctgaact gccggaggac aaacctgccc
     8941 gccggtggtg gccgttcggc aaaaaataaa tgccagcacc ggccatgccg gtgctgttat
     9001 tcctccccga gcttatcccg catcacctgc cagagtgttt ccgcatcctc gtgtagccgc
     9061 tcgcacatgt cggccggggt atccatatcc agctcattca gtttatcgag caatagcagc
     9121 gactgctcct taataaattt agccatattg agggctgttt tttcctgttt tgtcatgatt
     9181 ctttccttcc gggcatggtg tgcgcctgtc aggtatactg tctcaaaaat agcggggtga
     9241 gacaatggcg tgggtgacgg tgagacaggc gacggaactg acgggtaaag cccg
//
