LOCUS       NZ_CP066090             5601 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Citrobacter koseri strain FDAARGOS_1029 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP066090
VERSION     NZ_CP066090.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357198
            Assembly: GCF_016128275.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Kerrigan,L., Long,C., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Kerrigan,L., Long,C., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.11.1
            Genome Coverage                   :: 1371.54x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,426
            CDSs (total)                      :: 4,307
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 27 of 55
            Pseudo Genes (incomplete)         :: 26 of 55
            Pseudo Genes (internal stop)      :: 12 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            Pseudo Genes (short protein)      :: 1 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066090.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1029"
                     /culture_collection="ATCC:BAA-895D-5"
                     /db_xref="taxon:545"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA:VA"
                     /collected_by="ATCC"
     gene            join(5341..5601,1..249)
                     /locus_tag="I6H86_RS22140"
     CDS             join(5341..5601,1..249)
                     /locus_tag="I6H86_RS22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_024131073.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGLSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            256..468
                     /locus_tag="I6H86_RS21910"
                     /old_locus_tag="I6H86_21910"
     CDS             256..468
                     /locus_tag="I6H86_RS21910"
                     /old_locus_tag="I6H86_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(845..973)
                     /locus_tag="I6H86_RS22175"
     CDS             complement(845..973)
                     /locus_tag="I6H86_RS22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1136..1360
                     /locus_tag="I6H86_RS21915"
                     /old_locus_tag="I6H86_21915"
     CDS             1136..1360
                     /locus_tag="I6H86_RS21915"
                     /old_locus_tag="I6H86_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1344..1610
                     /locus_tag="I6H86_RS21920"
                     /old_locus_tag="I6H86_21920"
     CDS             1344..1610
                     /locus_tag="I6H86_RS21920"
                     /old_locus_tag="I6H86_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1680..2030)
                     /locus_tag="I6H86_RS21925"
                     /old_locus_tag="I6H86_21925"
     CDS             complement(1680..2030)
                     /locus_tag="I6H86_RS21925"
                     /old_locus_tag="I6H86_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2096..2641)
                     /locus_tag="I6H86_RS21930"
                     /old_locus_tag="I6H86_21930"
     CDS             complement(2096..2641)
                     /locus_tag="I6H86_RS21930"
                     /old_locus_tag="I6H86_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197758.1"
                     /translation="MFFKRHLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2667..3032)
                     /locus_tag="I6H86_RS21935"
                     /old_locus_tag="I6H86_21935"
     CDS             complement(2667..3032)
                     /locus_tag="I6H86_RS21935"
                     /old_locus_tag="I6H86_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226331160.1"
                     /translation="MRNKKRNSEGGGVMRERTNIMLDIGLKQSLQRLSNKTKMSLSDLI
                     NQRLSGSLSVEERIGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINE
                     MMVLKEYFRAKFYDEIK"
                     /name="hypothetical protein"
     gene            complement(3355..3513)
                     /locus_tag="I6H86_RS22205"
     CDS             complement(3355..3513)
                     /locus_tag="I6H86_RS22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4153..4329
                     /locus_tag="I6H86_RS21940"
                     /old_locus_tag="I6H86_21940"
     CDS             4153..4329
                     /locus_tag="I6H86_RS21940"
                     /old_locus_tag="I6H86_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4319..4663
                     /locus_tag="I6H86_RS21945"
                     /old_locus_tag="I6H86_21945"
     CDS             4319..4663
                     /locus_tag="I6H86_RS21945"
                     /old_locus_tag="I6H86_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4872..>5306
                     /locus_tag="I6H86_RS22145"
                     /pseudo=""
     CDS             4872..>5306
                     /locus_tag="I6H86_RS22145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3987..4067
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.095"
                     /coverage="98.0"
                     /evalue="6.71e-19"
     Typing          complement(3405..3802)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          4..584
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.42e-98"
     Typing          4654..5600
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgttcgcca gcctgagcgg ggtgttgtgg tatcagggga gcctgatagc gtcaaatctg
       61 gccgaaatcg accggcagaa cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg
      121 acgtacctgg aagacagcaa cggacgtttt ctggtgctgc cgaaggggac ggccgtagac
      181 aggacgcaga gctggacggt cgggaacggg ctgtcgaagc agaacgcgct gagactggtg
      241 aaggagtaag tgccgatgac cgagctggaa aaacagttgc tgagcgcatt agagcagcta
      301 cagcaggact actcgaaaag gctggacgag tgggagagcg ccttcgggga atggcggaaa
      361 atgtcagggc ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt
      421 cagcaggtgg aacgcttgag cgggcaactg cagcgtttga gccggtagtg cagcgtcatg
      481 agatggccgt ggccgcagaa cgggcgcacg agcaacggca gcacgaaaag gaactgacgg
      541 aagcgcgttc ccggcagcga agctatgacg gtccgtcgct gggctgaaat gcaggaaaac
      601 agggttctca tcgggcattt acgctgaaac cggatgtcat tcacctggct gcatggctat
      661 gcagccaggt aaaatttatc tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg
      721 ttttacctga ctgccacccc gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt
      781 gttatgttta atcagcgtga acggcagata caaaaaaagc cccggagggc ttttaactac
      841 tgacttattt ttttgaagat ttgctgggtt tcaatttgtc agtatttccc ggcgtttttt
      901 tattgtcacg ctggcgttta tcaggttccc ctgttgaatc ggcaattcgt ttcggacccg
      961 gcttaagacc cataatcgtt atcctttcaa tagttaagag gaaagccctc aagctagata
     1021 tattgaccgc gttacggatt atcaagaggg gctaacaaga ttattttttg ttcttttagg
     1081 ttgattgtgg tactgttgct atacatgtat agcaaatggt gttcgggagg tttatatgct
     1141 tgctatccgg ttatctgatg agattgagtc ccgtctggac tcgctggcga agcaaaccgg
     1201 cagaacaaag acgttttatg cgcgggaagc aatactggcg catctggaag acctggagga
     1261 ttattatctt tcagcagaaa ctgctgcacg cgttcgccgt ggtgatgaag cagtgcattc
     1321 gtctgaagac gtgaggaagt cacttggtct ggacgattaa ctattccgat cgggcgctca
     1381 aatcgttacg caagatggac aaacagaacg cacgacggat tgtggatttt atgagtttac
     1441 gcattgcagt tgctgccgat cctcgccagt cagggaagcc gctcaaaggt gagctgggcg
     1501 agttctggcg ctatcgggtg ggagattatc gcgttctgtg tgagatccga gatgacgagc
     1561 ttgttatcct tgccgccacg attggacatc gccgcgaagt ttacgactga agcccgctat
     1621 gcgggctttt ttgatgccga caacaggtat tatggtaaat actcgactaa ctacgtgttc
     1681 tactgtttgt cggttttagt taaccagcag gttgtcttaa cgataatgta gccgttattt
     1741 ttaactaata aggtcatatt gttaacgtca ggcataataa aagacattat ggcctcagag
     1801 ttatcatcag aagcagctct ctgcattact acttcgattc ccttttcttt gaatctataa
     1861 gtatccagtt gcctattaaa cttagaaaga cctacaacca tacttagttt gttatcttta
     1921 acagtaagag atgcttcctg agtaccatta catcggtagt aagcggtgta accgtcagca
     1981 aaagatcgac cggggaatat aaatgctagg aacgcaatta accaatacat agttgagaat
     2041 cttggtatta cacatttcac tatcctctct ttcatttttc gttcaccata ctttgttatc
     2101 tgatttttaa tttttctatt ttataaacca agtccctacc aaaaaggcga aaattatcct
     2161 caaataataa taattgttta agaacatcca tgaaagcagc tgcttcaccc tcagttatgg
     2221 gagctactct atataacata taacctgagt taagggtttc ttttatactt tcttcaatgc
     2281 tcattataaa tattagtgga ttatcatttt tcttccccca tgggtttcta attaatcctt
     2341 cgatgtactc aggtcctttg cgtcctattg cctgcgcaat attttttatt tcatctgcgt
     2401 gagggctttg ttgtacagct ttgtttatgc ctgcttgagt gattttcaga gactctacac
     2461 cgtgtatgtt gtatgagata aaatgtaata aattaagatg taatgcaatt aatccatttt
     2521 tgtttacatc tgagtatttg gataaaaaac gtaatgaagt gttaactctc ccttgaagcc
     2581 taacaattgg gagtgctgcc ttgtatattt ccttctccgt taaatgccgt ttaaaaaaca
     2641 taattctccg cctccgtata gcgtgtctat tttatttcgt cgtaaaactt agctctgaaa
     2701 tattccttta gaaccatcat ttcattaata attgaatcta tctcatccat tttttcttgg
     2761 tattctttta atggtgaagg gagcggtaac tcaatatttg aaaagtgatt ggcgagatag
     2821 ggatcaaggt ttctcatcca ttcaggcttt tcgccaatgc gttcttcaac tgaaagtgaa
     2881 cctgataatc tctggttaat aagatcactt agactcattt tggttttatt cgaaagccgt
     2941 tgtaaggatt gctttaatcc tatatctagc atgatatttg ttctttctct cattacccct
     3001 cccccctcgc tattgcgttt tttgttgcgc acaaagctac cattgcgcat taagaaacgc
     3061 aatgaaaaag gagggaggga gtggtttgta catgatgtgc agtgcgctga gggcatcagt
     3121 ggcgggtgtt tttccgtggg atggatggag ctccggggat aacagaactg acgatcattt
     3181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct cttgcactgt aaacgaaaaa
     3241 accgcctggg gaggcggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccgggt
     3301 aactgggtat acgagaccgc agataccaaa actgttcttt cagtgtagcc gcagttaggc
     3361 acccacttca agacctctca atatctctct cgtatatatc cagttaccaa tggctgctgc
     3421 cagtggcgtt tttgcgtgtc cgtccgggtt ggactcaaga tgatagttac cggaaagggc
     3481 gcagcagtcg ggctgaacgg ggggttcgtg catacagccc agcttggagc gaacgaccta
     3541 aaccgagccg agataccagc agcgtgagct atgagaaagc gccacgcttc ccgaaaggga
     3601 aaaaggcgga acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3661 ccgccggaga gaaatcaccg gcatctttaa gtcctgtcgg gtttcgtcct cctctgactt
     3721 gagcgtccat ttctgtgatg ctcgtcaggg gaggcggagc ctatggaaaa acggccacgc
     3781 cgcatccttt tctccggcac tttctgattt gagcgccttt gctttatccc gttatccgtg
     3841 agccggtcac gcccgccgca gccgaacggc aggagcgcag cgaccgagtg agcgaggagg
     3901 cgtcatttca tctgtccgtg catggtattg cggacgctcc tgagccacat atcacacctg
     3961 ccacatgagg cactttcatg aatcgcgctc tcatgtcagc atagtaagcc agtatacaca
     4021 gcgtaaacgc tgtgactggt tcagggctgc gccccgaaac ccgctaacac cactgacgcg
     4081 ctggcgcttg tccaacgccg gagcgacccg gaaaacattt ccgtgccgtc cggcgtttgc
     4141 gaaggaggga taatgcagaa gccgaagaag aagccgctcg tcgtaaaaaa aagatcctgg
     4201 catccggcag tgatggcaaa tctgatgcgt caggcacagg aggaagatta ccgccgggag
     4261 cagatggcag agcttgagcg ttcagcccgg tcacattttg cggatatcgg aggagaggat
     4321 ggcggttaag cgtacccgtt ttttaggtat ccgggtgacc gatggggagt accagcagct
     4381 gctggagcgc tgcaatggaa ggcaactggc agtgtggatg cgggaaacct gtctcgatac
     4441 gcgcccggcg cggtcgttgc ggctgccgtc catcgatccg gttttgctgc gccagcttgc
     4501 cgggatgggg aacaacctca accagatcgc ccggaaaatt aacggcggcc agtggtcagg
     4561 cgctgaccgg gttcaggtgg tggccgcgct gatggccatc gatgccggac tcgagcggct
     4621 gcggcatgcc gtgctggaaa agggggcaga cgatgatcgt taagtttcat ccgcgagggc
     4681 gaggcggcgg tgccgggccg gtggattatc tgctggggaa agaccgccag cgcgacgggg
     4741 ccatcgtcct gcagggaaag ccggaggagg tccgggagct tatcgacgcc tcgccctacg
     4801 ccaaaaagta cacctccggc gtcctgtctt ttgctgaaca ggatttaccg cccggccagc
     4861 gtgaaaagct gatggcgagc ttcgagcggg ttctgatgcc cggactcgat aaagaccagt
     4921 acagcgtgct gtgggttgaa caccgggaca aggggcggct ggaactgaat tttctggtac
     4981 cgaacacgga actgctgacc ggcaggcgtc tccagccgta ctacgaccgg gcagaccgtc
     5041 cgcgcatcga tgcgtggcag accgtggtga acgggcgact ggggctgcac gacccgaacg
     5101 caccggagaa ccggcgcgcg ctggtgacgc catcttcgtt acccaaaacg aagcaggaag
     5161 ccgcagaggc tattacgcgg ggcttactgg ccctcgcctc gtcaggggag cttaaaacgc
     5221 gtcaggacgt cactgaggcg ctggaaagcg caggttttga ggtcgtgcgc accacaaaga
     5281 gcagcatcag cattgccgac ccggacggag ggcgaaacat ccgacttaag ggagccatct
     5341 atgaacagtc ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag
     5401 agtaccggcg aaatgctgaa agccgcattc agcgagcacg agaagtctgt cagagcggaa
     5461 ctgagcgaaa gcgggaagag aatcagcgcc gccatccgcg cccacgagca gggtatgagc
     5521 gcggccatgc agtcgaaccg cctgagcgtg atgcgtatgg tcgggcggac atggctgacc
     5581 atcacgatgg tgtcaggact g
//
